• 한국산림과학회지
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• 제102권3호
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• pp.446-454
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• 2013

본 연구에서는 환경부 멸종위기종 2급인 나도승마 서식지의 생물학적 인자와 입지환경 조건을 조사하여, 중요치 및 다양도 지수에 의해 서식지 임분의 구조적 특성을 밝히고 요인별 비교분석 하여 환경요인과의 관계를 고찰하였다. 나도승마 자생지의 향 분석 결과 방위는 북 또는 북동으로 분포하고 있었으며, 경사는 15~40o로 경사에 대해서는 크게 영향을 받지 않는 것으로 조사 되었다. 또한 해발 600 m 이상 지역에서부터 나도승마 개체군의 분포가 급격히 증가하고 있음을 보여 주고 있었고, 상대광도는 평균 3.79%로 나타났다. 대부분의 개체군이 계곡부와 바위틈에 많이 자생하고 있어서 강우 시 토양유실이 우려되는 곳에 많이 서식하는 경향을 보이고 있었고 상당수 개체군은 등산로와도 인접해 있어 인위적인 훼손이 우려되고 있다. 나도승마의 주된 생육공간내 A층의 토양을 분석한 결과 pH 4.45에서 4.89(평균 4.68)로 강산성으로 분석되었다. 모든 조사구들은 전형적인 천연활엽수 지역이었으며, 신갈나무군집에서 나도승마의 출현확률이 가장 높은 것으로 보아 신갈나무가 나도승마 서식에 가장 큰 영향을 미치는 수종으로 해석되어진다.

• 환경영향평가
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• 제26권6호
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• pp.508-523
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• 2017

본 연구는 매노천 생태하천복원을 위한 생태적수질정화비오톱 시스템과 생태어도(Fish-way) 등의 적용 대상지를 중심으로 생태하천 복원 전 후를 모니터링하였다. 어류는 복원전 서식이 확인되지 않았던 생태적수질정화비오톱 습지에서 총 11종 191개체가 창출복원된 것으로 조사되었다. 특히 복원목표종인 돌마자와 참종개가 생태적수질정화비오톱 습지에서 서식함이 모니터링되어, 미소서식처와 건전한 Fish-way가 창출되었음을 확인할 수 있었다. 양서류는 복원후 활동성이 높은 3차 조사시기에 습지와 그 주변에서 다수의 참개구리 서식으로 복원되었음이 확인되었다. 포유류는 수달이 습지와 Fish-way를 서식영역으로 활용하는 것이 확인되어 환경부 멸종위기 제1급이자 천연기념물인 수달 서식지가 복원된 것으로 조사되었다. 식물상의 경우 조사지역에 출현하는 관속식물은 복원 전 총 7과 13종, 복원직후 15과 19종, 복원후 총 22과 33종으로 증가되었다. 복원후 식생은 달뿌리풀 군락 등의 다양한 생태계의 기초생산자이자 수질정화에 기여하는 식재종인 정수식물군락이 형성된 것으로 조사되었다. 수질 모니터링 결과, 평균적으로 BOD 64.3%, T-N 47.2%, T-P 80.7%의 처리효율을 나타내었다. 생태계를 교란하는 제한요인(limiting factor)이 되는 비점오염원이 성공적으로 처리됨으로써 I, II등급수질에 서식하는 목표종이 창출적으로 복원되었다.

• 한국콘텐츠학회논문지
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• 제21권5호
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• pp.488-498
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• 2021

본 연구는 유튜브를 활용한 기초조리실습교과목에 플립드러닝 교수학습방법을 적용하여 학습 전과 후에 따른 효과를 파악하고 학습과정을 통해 학습자의 주관적인 인식을 분석하여 교육과정이 적절히 진행되고 있는지에 대해 연구하고자 한다. 조사기간은 2020년 08월 01일부터 09월 10일까지 진행되었으며, Q방법론의 연구 설계에 따라 Q표본 선정, P표본 선정, Q소팅, 코딩과 리쿠르팅, 결론 및 논의로 총 5단계로 구분하여 진행하였다. 분석결과 제 1유형(N=5) : 선행학습 효과(Prior Learning effect), 제 2유형(N=7) : 시뮬레이션실습효과(Simulation practice effect), 제 3유형(N=3) : 자기효능감 효과 (self-efficacy effect)로 각각 고유의 특징을 가진 유형으로 분석되었다. 결과적으로 유튜브를 활용한 기초조리실습과목의 플립드러닝 교수학습방법을 적용함으로서 적극적인 학습자들에게는 수업의 흥미유발, 자신감 상승 등의 긍정적인 효과가 나타났으나 일부 학습자의 경우 수업운영방식의 시스템이해 부족, 타 과목에 비해 실습회수 부족 등은 추후 해결되어야 할 방안으로 사료된다.

• 한국가정과교육학회지
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• 제24권1호
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• pp.21-36
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• 2012

본 연구는 학교교육에서 여고생을 대상으로 한 식생활교육의 방향을 알아보기 위해 대구 경북지역 1, 2학년 여자고등학생 409명을 대상으로 영양지식, 식습관, 식생활교육 수업에 대한 만족도, 가정교과의 식생활교육이 실생활에서 도움을 주는 정도에 대하여 자기기입식 설문조사로 실시되었다. 영양지식 점수는 9.2/15.0점으로 계열, 학년, 형제 수, 성적과 유의적 상관관계를 나타내었다(p<0.05). 아침식사(85.8%), 적절한 식사속도(82.9%) 항목의 점수는 높았으나 병원성 미생물(43.0%), 열량영양소(40.3%), 당뇨병 식이관리(21.3%) 항목은 매우 낮은 정답률을 보였고, 쌀의 조리원리와 병원성 미생물에 대한 항목은 잘 모르겠다고 응답한 학생이 각각 51.8%, 49.1%로 가장 높았다. 식습관 점수는 3.3/5.0점으로 계열, 부의 직업, 경제적 수준과 유의적인 차이를 보였다(p<0.05). 식품구매 시 영양표시 및 유통기한, 원산지 확인 항목의 점수가 가장 높았으나(3.86/5.00점) 싱겁게 먹기 항목은 2.93/5.00점으로 가장 낮았다. 식생활교육 수업에 대해서는 42.1%가 만족하고 있었으나 8.3%가 불만족한 것으로 조사되었고, 불만족한 가장 큰 이유는 수업시간이 적다가 8.3%로 가장 높았다. 실험 및 실습수업을 가장 선호하였으며, 식생활교육 만족도 점수는3.39/5.00점, 식생활교육이 실생활에 도움을 주는 정도도 3.39/5.00점으로 대체로 낮았고, 음식 만들기(3.81점), 위생적이고 안전한 식생활(3.71점), 건강한 생활(3.68점) 항목점수가 높았다. 식생활교육 수업에 대한 만족도가 높을수록 실생활에 도움이 되는 정도, 영양지식, 식습관의 점수가 유의적으로 높았으며(p<0.01), 영양지식과 식습관 점수가 높을수록 실생활에서 도움이 많이 된다고 생각하였고(p<0.001), 영양지식 점수가 높을수록 식습관 점수가 높은 것으로(p<0.001)로 조사되었다. 따라서 여고생을 대상으로 한 가정교과의 식생활교육은 학부모와 연계하여 실생활에서의 활용도를 높이고, 학생의 진로와 요구를 반영한 효과적인 수업전략 및 식생활교육 시간 확보 등으로 식생활교육 만족도를 높이기 위한 교사의 적극적인 활동이 요구되었다.

• 한국가정과교육학회지
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• 제12권2호
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• pp.81-93
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• 2000

This study is designed to identify the current condition of male students ostracized as the black ship (Wangtta in Korean) in middle schools and the effects of the Guardian Angle program on the decrease in Wangtta. 428 frist graders of two junior high schools were devided into two groups, a test group (n=195) and a control group (n=233). SPSS/PC+ program was used for analysis of the data, including frequency analysis, ANOVA and x(sup)2-test. The outcome of research can be summed up as the following 74.7% of the subjects thinks that there is Wangtta going on in there classes. Wangtta often comes to pass during the recesses (74.2%) and lunch times (51.6%). Those who are easily exposed to Wangtta are most likely to be both snobbish and pedantic. Those who lead Wangtta turn out to be good at fighting (56.4%) and dominant in the hegemony of the class depending on the way of ignoring or not playing with the victims(69.3%) In the case of bearing witness to Wangtta. 36.9% of the subjects try to overlook the case. Now let me take a look at the effect of the guardian angle program on the decrease in Wangtta. The program has a significant effect on the decrease (p<0.05) in the number and the phenomena of Wangtta perceived by students in both advance and post tests. The program makes a significant difference in the aspects of students’ behavior in relation to Wangtta in a desirable way (p<.01) Though the sociality index of the victims doesn’t show any significant difference statistically, it exerts a generally positive effect.

• 대한지역사회영양학회지
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• 제10권6호
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• pp.920-929
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• 2005

This study was aimed at knowing the recognition of teachers' nutrition knowledge and nutrition education at day-care centers in Seong-nam. Teachers were all female, most of whom were in the 20, and their careers were less than 3 years. And $76.2\%$ of the teachers graduated from high school and junior college. The score of the nutrition knowledge was average $14.3\pm2.2$ (out of 20). It shows that they are lack of general information of nutrition. As the teachers had higher education, they scored higher nutrition knowledge levels (p < 0.01). It also says that there was meaningful interrelationship between the nutrition knowledge and the number of children they had (p < 0.05). $93\%$ of the teachers said that nutrition education should be given when children are three years old, at least and most of the teachers thought that nutrition education is necessary and should be taught in a separate course. $50.4\%$ of the teachers thought that nutrition education for early childhood should be taught by a nutritionist, but $35.2\%$ of them thought that the teachers themselves should be in charge of it. About $60\%$ of the teachers thought that 'Cooking Activity for Early Childhood' course should be established and it is desirable that the nutrition education should be in the area of cooking activity for childhood at day-care centers. The teachers thought that dietary habit is the most important subject in nutrition education and they got more information from the internet rather than in the class related to nutrition. (Korean J Community Nutrition 10(6) : $920\∼929$, 2005)

• Journal of Nutrition and Health
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• 제40권1호
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• pp.89-96
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• 2007

This study was performed to weigh the average meal portion sizes served for preschoolers by kindergarten teacher. The subjects were 53 teachers from 8 kindergartens, which are random sampled by meal service number. Using the weighing method assessed the meal portion sizes of food items at lunch. The data was complied by performing $\chi^2-test$ using SPSS WIN 11.0. The result was as followed: 98.0% of teacher agreed with the meal service because of 'better food habit and table etiquette'(68.0%), 'health promotion with balanced diet'(22.0%), 'owing to extending school time'(6.0%) and 'demand of parents'(2.0%). Preschooler eat lunch at class (84.9%) and meal serving size was decided by teacher (79.2%). Teachers thought that they know very well about portion size 3.8%, 96.2% of teacher thought that they don't know much about portion size. Portion size were not significantly different by food tray types but there was much different (almost 100%) compared with minimum and maximum within dishes. Most average portion size was not met dietary reference intake except cooked rice, soups and fish cutlet. Working experience effected on portion size. More served, more working experienced of teacher. For example Bulgogi was served 26.8 g by teacher who has over 6 years working experience compared with 2-6 years (20.4 g) and less than 2 years (17.1 g) (p < 0.01). Spinach portion size was significantly different by teacher's working experience (p < 0.01). Portion size were not significantly different by preschooler's age. The reference of dietary for preschooler was different by age, but teachers served meal by their experience. According to the results of this study, it is necessary to educate meal portion size for kindergarten teacher who take charge in meal serving. To provide guidance to teacher about reasonable portion sizes for preschoolers, teacher need to take nutrition education about meal service and child nutrition in college. This study would be useful to those who plan meals for preschoolers and to researchers studying dietary intakes of preschooler.

• IMMUNE NETWORK
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• 제10권3호
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• pp.99-103
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• 2010

Background: Glycogen synthase kinase $3{\beta}$ ($GSK3{\beta}$) is a ubiquitous serine/threonine kinase that is regulated by serine phosphorylation at 9. Recent studies have reported the beneficial effects of a number of the pharmacological $GSK3{\beta}$ inhibitors in rodent models of septic shock. Since most of the $GSK3{\beta}$ inhibitors are targeted at the ATP-binding site, which is highly conserved among diverse protein kinases, the development of novel non-ATP competitive $GSK3{\beta}$ inhibitors is needed. Methods: Based on the unique phosphorylation motif of $GSK3{\beta}$, we designed and generated a novel class of $GSK3{\beta}$ inhibitor (GSK3i) peptides. In addition, we investigated the effects of a GSK3i peptide on lipopolysaccharide (LPS)-stimulated cytokine production and septic shock. Mice were intraperitoneally injected with GSK3i peptide and monitored over a 7-day period for survival. Results: We first demonstrate its effects on LPS-stimulated pro-inflammatory cytokine production including interleukin (IL)-6 and IL-12p40. LPS-induced IL-6 and IL-12p40 production in macrophages was suppressed when macrophages were treated with the GSKi peptide. Administration of the GSK3i peptide potently suppressed LPS-mediated endotoxin shock. Conclusion: Collectively, we present a rational strategy for the development of a therapeutic GSK3i peptide. This peptide may serve as a novel template for the design of non-ATP competitive GSK3 inhibitors.

• 한국동물위생학회지
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• 제30권1호
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• pp.51-66
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• 2007

The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.81-88
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• 2012

Histone deacetylases (HDACs) are enzymes involved in the remodelling of chromatin, and have a key role in the epigenetic regulation of gene expression. Histone deacetylase (HDAC) inhibitors are emerging as an exciting new class of potential anti-cancer agents. In recent years, a number of structurally diverse HDAC inhibitors have been identifi ed and these HDAC inhibitors induce growth arrest, differentiation and/or apoptosis of cancer cells in vitro and in vivo. However, the underlying molecular mechanisms remain unclear. This study aimed at investigating the anti-tumor activity of various HDAC inhibitors, IN-2001, using T47D human breast cancer cells. Moreover, the possible mechanism by which HDAC inhibitors exhibit anti-tumor activity was also explored. In estrogen receptor positive T47D cells, IN-2001, HDAC inhibitor showed anti-proliferative effects in dose-and time-dependent manner. In T47D human breast cancer cells showed anti-tumor activity of IN-2001 and the growth inhibitory effects of IN-2001 were related to the cell cycle arrest and induction of apoptosis. Flow cytometry studies revealed that IN-2001 showed accumulation of cells at $G_2$/M phase. At the same time, IN-2001 treatment time-dependently increased sub-$G_1$ population, representing apoptotic cells. IN-2001-mediated cell cycle arrest was associated with induction of cdk inhibitor expression. In T47D cells, IN-2001 as well as other HDAC inhibitors treatment significantly increased $p21^{WAF1}$ and $p27^{KIP1}$ expression. In addition, thymidylate synthase, an essential enzyme for DNA replication and repair, was down-regulated by IN-2001 and other HDAC inhibitors in the T47D human breast cancer cells. In summary, IN-2001 with a higher potency than other HDAC inhibitors induced growth inhibition, cell cycle arrest, and eventual apoptosis in human breast cancer possibly through modulation of cell cycle and apoptosis regulatory proteins, such as cdk inhibitors, cyclins, and thymidylate synthase.