Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Effect of porcine reproductive and respiratory syndrome virus on pigs with pleuropneumonia - Studies on serology and proportion of porcine subpopulation of peripheral blood -

돼지생식기호흡기증후군 바이러스가 흉막폐렴에 미치는 영향 - 혈청학적 및 백혈구아군 분포율에 관한 연구 -

  • Jeong, Hyun-Kyu (Dodram Pig Farmer's Co-operative) ;
  • Park, Yong-Ho (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Han, Jeong-Hee (Department of Veterinary Pathology, School of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University)
  • Published : 2007.03.30
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Abstract

The purpose of this study was to evaluate the effect of a subsequent infection of porcine reproductive and respiratory syndrome(PRRS) virus to pigs with A pleuropneumonia. Twenty three 7-week-old commercial pigs were infected intratracheally with PRRS virus and/or A pleuropneumoniae serotype 5. Serum antibody titers were examined by an enzyme-linked immunosorbent assay(ELISA) and proportion of porcine leukocyte subpopulations in peripheral blood was examined by flow cytometry. In this experiment, antibodies against PRRS virus and A pleuropneumoniae were detected at 2 weeks and 1 week postinfection and the number of antibody positive pigs were gradually increased. And in proportion to leukocyte subpopulations in peripheral blood of pigs infected with A pleuropneumoniae compared with pigs administrated with saline, the proportion of PoCD4 and N cells were increased(P<0.1). Furthermore, in proportion to leukocyte subpopulations in peripheral blood of pigs infected with PRRS virus followed by A pleuropneumoniae compared with pigs administrated with saline, the proportion of MHC class II, PoCD4 and B cells were significantly increased(P<0.1). The results indicated that dual infection with PRRS virus and A pleuropneumoniae induced the stronger immune responses associated with macrophages and Th cells in pigs than single infection with PRRS virus or A pleuropneumoniae.

Keywords

References

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