• 제목/요약/키워드: optimal growth condition

검색결과 543건 처리시간 0.024초

재래식 메주로부터 효모의 분리, 동정 및 배양조건

  • 이종수;이성훈;권수진;안철;유진영
    • 한국미생물·생명공학회지
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    • 제25권5호
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    • pp.435-441
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    • 1997
  • Forty seven strains of yeast were isolated from traditional Meju and were identified as Saccharomyces spp. (7 strains), Zygosaccharomyces spp. (7 strains), Kluyveromyces spp. and Hansenula spp. (each 5 strains), Rhodotorular spp. (8 strains), Candida spp. (12 strains), Pichia spp. and Debaryomyces spp. from results of their microbiological characteristics. The optimal medium for growth of all the yeasts was YM media and the optimal initial pH of the medium was 6.0. The optimum temperature for growth was 30$circ$ and among them, Sacch. exiguus OE-5, Sacch. cerevisiae OE-16, Sacch. kluyveri C-1 strains were thermotolerant yeasts which could grow at 40$circ$C.

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A Prediction of Nutrition Water for Strawberry Production using Linear Regression

  • Venkatesan, Saravanakumar;Sathishkumar, VE;Park, Jangwoo;Shin, Changsun;Cho, Yongyun
    • International journal of advanced smart convergence
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    • 제9권1호
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    • pp.132-140
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    • 2020
  • It is very important to use appropriate nutrition water for crop growth in hydroponic farming facilities. However, in many cases, the supply of nutrition water is not designed with a precise plan, but is performed in a conventional manner. We proposes a forecasting technique for nutrition water requirements based on a data analysis for optimal strawberry production. To do this, the proposed forecasting technique uses linear regression for correlating strawberry production, soil condition, and environmental parameters with nutrition water demand for the actual two-stage strawberry production soil. Also, it includes predicting the optimal amount of nutrition water requires according to the heterogeneous cultivation environment and variety by comparing the amount of nutrition water needed for the growth and production of different kinds of strawberries. We suggested study uses two types of section beds that are compared to find out the best section bed production of strawberry growth. The dataset includes 233 samples collected from a real strawberry greenhouse, and the four predicted variables consist of the total amounts of nutrition water, average temperature, humidity, and CO2 in the greenhouse.

Y$Ba_{2}$$Cu_{3}$$O_{x}$단결정의 용융온도 및 유지시간에 따른 물리적 특성 변화 (Physical properties with melting temperature and holding time for $YBa_{2}$$Cu_{3}$$O_{x}$ single crystal)

  • 박병삼;한상철;한영희;정년호;윤희중;김경진;오제명;성태현
    • 한국초전도저온공학회:학술대회논문집
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    • 한국초전도저온공학회 2003년도 추계학술대회 논문집
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    • pp.93-95
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    • 2003
  • YBa$_2$Cu$_3$O$_{x}$ (Y123) single crystal was grown by TSMG (top seeded melt growth) method. Physical properties of Y123 single crystal were dependent on the shape and distribution of Y2BaCuOx (Y211) in it and on the oxygenation temperature and the holding time. In this work, It was investigated to an optimal oxygenation condition and a dependence of melting temperature and holding time on physical properties. The optimal oxygenation condition was found that it was heat-treated for 30 hours at 45$0^{\circ}C$, Also it was found that the critical current density and the size of Y211 were increased with the melting temperature and the holding time.e.

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버들송이의 균사배양조건 및 최적 접종량 설정 (Condition of mycelial culture and inoculum volume of spawn on cultivation of Agrocybe cylindracea)

  • 이기권;유영진;최규환;정종성
    • 한국버섯학회지
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    • 제10권1호
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    • pp.21-28
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    • 2012
  • 본 연구는 버들송이버섯의 균사 생육 최적조건을 구명하여 액체종균을 제조하고 또한 톱밥종균의 영양원을 선발하기위하여 시험을 수행하였고, 선발된 종균에 대한 종균 접종량을 설정하여 병재배에 적합한 조건을 구명하고자 하였다. 버들송이버섯의 톱밥종균은 미송톱밥(70%)+밀기울(30%)의 비율, 포플러톱밥(80%)+옥수수가루(20%) 조건으로 배합할 때 균사 생육이 양호하였다. 액체종균에 대한 영양원 선발 및 첨가량은 백설탕 1.0~1.5%, yeast extract $0.7g/{\ell}$, 콩가루 $0.17g/{\ell}$, 무기물은 $MgSO_4{\cdot}7H_2O\;0.3g/{\ell}$, $KH_2PO_4\;0.5g/{\ell}$, $K_2HPO\;1.2g/{\ell}$가 최적 조건이었다. 이때 선발된 배지의 접종량은 액체 및 톱밥종균을 $25m{\ell}/850m{\ell}$$20{\sim}25g/850m{\ell}$로 접종 할 때 자실체 발생이 양호하였다.

Culture conditions for mycelial growth of Poria cocos

  • Jo, Woo-Sik;Park, Ju-Ri;Oh, So-Ra;Kang, Min-Gu;Kim, Woo-Hyun;Park, Seung-Chun
    • 한국버섯학회지
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    • 제14권1호
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    • pp.6-13
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    • 2016
  • This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.

Effects of dietary protein and energy levels on growth and body composition of juvenile turbot (Scophthalmus maximus L.) reared under optimal salinity condition

  • Cho, Sung-Hwoan;Lee, Jong-Kwan;Lee, Jong-Ha;Lim, Young-Soo;Lim, Han-Kyu;Lee, Jung-Ho;Lee, Sang-Min
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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    • pp.239-241
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    • 2003
  • Several nutrients in feed are needed for growth and maintenance of fish and protein among them is probably the most important factor influencing its growth and determining feed cost due to high Proportion. Besides, dietary energy level is critical because protein source in the feed was utilized as energy source when the feed deficient in energy was fed to fish, whereas when the feed excess in energy was fed to fish, feed consumption decreased and resulted in growth reduction due to lack of necessary other nutrients for the normal growth (Lovell 1989). (omitted)

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Effect of surface roughness on the quality of silicon epitaxial film grown after UV-irradiated gas phase cleaning

  • Kwon, Sung-Ku;Kim, Du-Hyun
    • 한국결정성장학회지
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    • 제9권5호
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    • pp.504-509
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    • 1999
  • In-situ cleaning and subsequent silicon epitaxial film growth were performed in a load-locked reactor equipped with Hg-grid UV lamp and PBN heater to obtain the smooth and contaminant-free underlying surface and develop low-temperature epitaxial film growth process. The removals of organic and native oxide were investigated using UV-excited $O_2$ and $NF_{3}/H_{2}$, and the effect of the surface condition was examined on the quality of silicon epitaxial film grown at temperature as low as $750^{\circ}C$. UV-excited gas phase cleaning was found to be effective in removing the organic and native oxide successfully providing a smooth surface with RMS roughness of 0.5$\AA$ at optimal condition. Crystalline quality of epitaxial film was determined by smoothness of cleaned surface and the presence of native oxide and impurity. Crystalline defects such as dislocation loops or voids due to the surface roughness were observed by XTEM.

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Phellinus baumii으로부터 세포외 다당체 생산의 최적화 (Optimal Conditions of Mycelial Growth and Exopolysaccharide Production in Submerged Culture of Phellinus baumii)

  • Hwang, Hye-Jin;Kim, Sang-Woo;Yun, Jong-Won;Park, Jang-Won
    • 생명과학회지
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    • 제14권1호
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    • pp.51-56
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    • 2004
  • 장수상황버섯(Phellinus baumii)의 균사체 성장 및 세포외 다당체(exopolysaccharides) 생산을 위한 액체배양의 최적 배지 및 배양조건에 관한 실험을 수행한 결과, 최적배양 온도와 초기 pH는 각각 3$0^{\circ}C$와 5.0으로 결정되었다. 탄소원의 경우, cellobiose와 maltose가 균사체 성장에는 양호하였으나 저조한 세포외 다당체 생산을 보였으며, 반면에 fructose 와 mannitol 은 상대적으로 높은 수율의 세포외 다당체 생산과 균사체 성장 또한 양호하였다. 최적 질소원으로는 yeast extract가 균사체 성장뿐만 아니라 세포외 다당체 생산에 효과적인 것으로 결정되었다. 최적 배지의 조성은 fructose 20 g/L, yeast extract 20 g/L 및 $CaCl_2$ 0.55 g/L이었으며, 최적 배양조건 하에서 5-L 교반 발효조를 운전한 결과, 최대 균사체 성장(17.43 g/L)과 최대 세포외 다당체 생산(3.6 g/L)을 얻을 수 있었다. 회수된 세포외 다당체는 glycoprotein이었으며 그 조성은 amino acid분석 결과 주로 arginine (14.1%)과 glycin (12.0%)이었으며, cabohydrate의 경우는 mannose (48.7%)와 arabinose (38.4%)이 주성분이었다.

꽃송이버섯(Sparassis crispa)의 균사생장 최적화 (Optimal condition for mycelial growth of Sparassis crispa)

  • 서상영;유영진;정기태;류정;고복래;최정식;김명곤
    • 한국버섯학회지
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    • 제3권2호
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    • pp.45-51
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    • 2005
  • 농촌진흥청 응용미생물과와 농가에서 수집한 꽃송이버섯 균주의 배양적 특성을 조사하였다. 기본배지는 감자 추출물을 이용한 PBA, PDA, PDM 배지에서 균사생장이 양호한 경향이었고, 특히 대나무톱밥 추출물이 함유된 PBA 배지에서 균사생장 및 밀도가 가장 좋았다. 꽃송이버섯 균사의 최적 배양온도는 $25^{\circ}C$이었으며, $30^{\circ}C$ 이상에서는 균사생장이 전혀 이루어지지 않았다. 최적 pH 조건은 모든 균주가 pH 5.0에서 균사생장 및 밀도가 양호하였다. 대부분 균주가 pH 4.0에서 균사생장은 좋았으나 밀도가 현저히 낮은 경향이었지만, SC12 균주는 pH 4.0에서도 균사생장이 양호하였다. 탄소원은 단당류인 glucose, fructose, mannose, xylose와 이당류인 maltose에서 균사생장이 양호하고 밀도가 높았다. 이중 가장 경제적인 탄소원 glucose를 1.0~1.5% 농도로 처리하였을 때 균사생장이 양호하였다. 질소원은 threonine, peptone, glycine, glutamine 그리고 valine을 첨가한 배지에서 균사생장이 양호하고 밀도가 높았으며, 질소원 peptone을 0.3% 처리하였을 때 균사생장 및 밀도가 가장 좋았다.

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환경조건에 따른 Aphanizomenon flos-aquae (Cyanophyceae) 균주의 성장 반응 및 독소 생성 (Response of Growth and Toxigenicity to Varying Temperature and Nutrient Conditions in Aphanizomenon flos-aquae (Cyanophyceae))

  • 류희성;신라영;이정호
    • 한국물환경학회지
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    • 제33권5호
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    • pp.538-545
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    • 2017
  • The purpose of this study is to investigate growth response and toxigenicity under various temperature and nutritional conditions, in order to understand the physioecological characteristics of Aphanizomenon flos-aquae, which is a bloom-forming cyanobacterium in the Nakdong River. The strain was inoculated into media under combinations of four temperatures (4, 12, 21, $30^{\circ}C$) and three nutrients (modified CB medium, P-depleted CB medium, N-depleted CB medium) for 28 days. The algae-inhibition tests were performed to assess the potential allelopathic effects of the strains' filtrates on the growth of four algae strains (Microcystis aeruginosa, Aulacoseria ambigua f. spiralis, Aphanizomenon flos-aquae, Scenedesmus obliquus). Toxin production of a strain was measured by Enzyme-Linked ImmunoSolbent Assay (ELISA). The optimal growth temperature (Topt) of strains was $19.9^{\circ}C$ ($18.3-21.2^{\circ}C$), and the temperature range for growth was from $-0.3^{\circ}C$ to $34.3^{\circ}C$. Specific growth rate (${\mu}$) in modified CB medium varied from 0.10 to $0.16day^{-1}$, and the maximum growth rate (${\mu}_{max}$) was $0.17day^{-1}$. Although growth curves under N-existed and N-depleted conditions were almost the same, growth under N-depleted condition was relatively slowed (${\mu}=0.09$ to $0.14day^{-1}$), with a decreased maximum cell density. However, growth under the P-depleted condition was restricted for all temperatures, Two stains of Aphanizomenon flos-aquae were confirmed as not producing toxins, because saxitoxin and cylindrospermopsin were not detected by ELISA. The exudates or filtrates from the Aphanizomenon flos-aquae (DGUC003) resulted in significant inhibition of algal growth on the Aulacoseira ambigua f. spiralis (DGUD001) and Aphanizomenon flos-aquae (DGUC001) (p < 0.01).