Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Culture conditions for mycelial growth of Poria cocos

  • Jo, Woo-Sik (Gyeongbuk Province Agricultural Technology Administration) ;
  • Park, Ju-Ri (Gyeongbuk Province Agricultural Technology Administration) ;
  • Oh, So-Ra (Gyeongbuk Province Agricultural Technology Administration) ;
  • Kang, Min-Gu (Gyeongbuk Province Agricultural Technology Administration) ;
  • Kim, Woo-Hyun (Gyeongbuk Province Agricultural Technology Administration) ;
  • Park, Seung-Chun (College of Veterinary medicine Kyugpook National University)
  • Received : 2016.02.29
  • Accepted : 2016.03.24
  • Published : 2016.03.31
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Abstract

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.

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References

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