• Journal of the Optical Society of Korea
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• 제19권1호
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• pp.69-73
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• 2015

The mesothelium is an essential lining for maintaining the normal homeostasis of the closed body cavity and a central component of pathophysiologic processes. The mesothelium has been known as the end target for asbestos which induces asbestos-related lung diseases. Malignant mesothelioma (MM) is a rare and fatal neoplasm predominantly due to asbestos exposure. Adaptation of an advanced and reliable technology is necessary for early detection of MM because it is difficult to diagnose this disease in its early stages. Optical coherence tomography (OCT) provides cross-sectional images of micro-tissue structures with a resolution of $2-10{\mu}m$ that can image the mesothelium with a thickness of ${\sim}100{\mu}m$ and, therefore, enable investigation of early development of MM. The mesothelium is typically located at the pleura and tunica vaginalis of the scrotum. In this study, we developed animal window models in the above two anatomical sites to visualize mesothelial layers within the mesothelium. OCT images at the two locations were also acquired.

• Journal of the Optical Society of Korea
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• 제18권5호
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• pp.551-557
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• 2014

In this study, we demonstrated multimodal nonlinear optical (NLO) microscopy integrated simultaneously with two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS) in order to obtain targeted cellular and label-free images in an immunofluorescence assay of the atherosclerotic aorta from apolipoprotein E-deficient mice. The multimodal NLO microscope used two laser systems: picosecond (ps) and femtosecond (fs) pulsed lasers. A pair of ps-pulsed lights served for CARS (817 nm and 1064 nm) and SHG (817 nm) images; light from the fs-pulsed laser with the center wavelength of 720 nm was incident into the sample to obtain autofluorescence and targeted molecular TPEF images for high efficiency of fluorescence intensity without cross-talk. For multicolor-targeted TPEF imaging, we stained smooth-muscle cells and macrophages with fluorescent dyes (Alexa Fluor 350 and Alexa Fluor 594) for an immunofluorescence assay. Each depth-sectioned image consisted of $512{\times}512$ pixels with a field of view of $250{\times}250{\mu}m^2$, a lateral resolution of $0.4{\mu}m$, and an axial resolution of $1.3{\mu}m$. We obtained composite multicolor images with conventional label-free NLO images and targeted TPEF images in atherosclerotic-plaque samples. Multicolor 3-D imaging of atherosclerotic-plaque structural and functional composition will be helpful for understanding the pathogenesis of cardiovascular disease.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2014년도 추계학술대회 논문집
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• pp.186-186
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• 2014

In this study, corrosion behaviors of Ti-xNb alloys for biomaterials. The Ti-xNb binary alloys contained from 10 wt. % to 50 wt. % contents were manufactured by vacuum arc-melting furnace. Microstructures of the alloys were examined by optical microscope (OM) and field emission scanning electron microscope (FE-SEM). In order to identify the phase constitutents of the Ti-xNb alloys, X-ray diffractometer (XRD) with a Cu K radiation was used. The corrosion behaviors were investigated using potentiostat (Model2273,EG & GCo, USA) in NaCl solution at $(36.6{\pm}1.0)^{\circ}C$.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2012년도 춘계학술발표회 논문집
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• pp.254-254
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• 2012

The purposed of this work was to determine surface charateristics of anodized Ti-30Nb-xTa alloys with Ta content. Samples were prepared by arc melting, followed by followed by homogenization for 12 hr at $1000^{\circ}C$ in argon atmosphere. The electrolyte for anodization treatment was prepared by mixing 465ml $H_2O$ with 35M $H_3PO_4$ and anodized at 180V to 220V. The microstructures of the alloys were examined by X-ray diffractometer (XRD) and optical microscopy (OM). Surface characteristics of anodized Ti-30Nb-xTa alloys was investigated by potentiodynamic test and potentiostatic in 0.9% Nacl solution at $36.5{\pm}1^{\circ}C$. It was observed that the changed ${\alpha}$ phase to ${\beta}$ phase with Ta content.

• Current Optics and Photonics
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• 제8권2호
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• pp.192-200
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• 2024

Wheat stripe rust, caused by Puccinia striiformis, has reduced winter wheat yield globally for ages. In this work, multi-spectral Mueller matrix imaging with 37 measurements using the method of double rotatable quarter-wave plates was used to investigate wheat stripe rust. Individual Mueller matrix measurements were performed on incident monochromatic light with nine bands in the range of 430 to 690 nm. As a result, it was found that the infected area absorbed linearly polarized light and was sensitive to circularly polarized light in the spectral domain. Both linear depolarization and linear diattenuation images distinguished between wheat stripe rust and healthy tissue. The responsiveness of stripe rust to polarized light reveals the potential of using polarization imaging to detect plant diseases. This further suggests that the multi-spectral Mueller matrix imaging system provides us with an alternative approach to agricultural disease detection.

• 한국의학물리학회지:의학물리
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• 제10권1호
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• pp.9-15
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• 1999

혈액에서의 산소 포화도는 Hb와 HbO$_2$의 광학 스펙트라에서 흡수계수의 차이에 의해서 측정할 수 있다. 본 연구는 하지 조직에서 발생하는 말초혈관계 질환진단을 위하여 파장이 660nm 와 940nm의 LED를 이용하여 산소포화도를 측정하는 장치를 개발하였다. 산소측정장치는 광 탐촉자와 광 신호처리부, LED 구동회로, 컴퓨터와 인터페이스로 구성하고, 데이터의 수집과 분석을 위한 프로그램 을 개발하였다. 구현된 산소측정장치에 대한 임상적인 평가를 위하여 하지조직에서 운동 부하에 따른 실험을 하여 생체조직내 생리적변화에 따른 산소량의 변화를 측정하고, 기기의 성능을 평가하였다. 실험결과 생체조직내의 산소포화도는 광원과 검출기의 간격에 따라 측정이 가능함을 보여 주었다.

• The Plant Pathology Journal
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• 제32권4호
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• pp.363-370
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• 2016

Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker disease in kiwifruit. Antibacterial activity of plant essential oils (PEOs) originating from 49 plant species were tested against Psa by a vapor diffusion and a liquid culture assays. The five PEOs from Pimenta racemosa, P. dioica, Melaleuca linariifolia, M. cajuputii, and Cinnamomum cassia efficiently inhibited Psa growth by either assays. Among their major components, estragole, eugenol, and methyl eugenol showed significant antibacterial activity by only the liquid culture assay, while cinnamaldehyde exhibited antibacterial activity by both assays. The minimum inhibitory concentrations (MICs) of estragole and cinnamaldehyde by the liquid culture assay were 1,250 and 2,500 ppm, respectively. The MIC of cinnamaldehyde by the vapor diffusion assay was 5,000 ppm. Based on the formation of clear zones or the decrease of optical density caused by these compounds, they might kill the bacterial cells and this feature might be useful for managing the bacterial canker disease in kiwifruit.

• 정보처리학회논문지B
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• 제10B권5호
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• pp.509-514
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• 2003

본 논문은 흉부를 촬영한 CT(Computed Tomography) 영상을 이용하여 기관지 내부를 시각화(visualization)하기 위해 가상기관지경(virtual bronchoscopy)을 구현하였다. 실제 광학내시경은 침습(invasive)검사로 환자가 불편함을 감수해야하며 검사 전에 사전 준비 작업이 필요하고 절개, 감염, 출혈 등의 부작용을 수반하는데 비해 가상내시경은 CT 나 MRI 등과 같은 스캐너의 단면 영상을 사용하여 구현한다. 가상기관지경의 항해경로 결정을 위해 CT 단면 원천영상에서 기관지(trachea wall)만을 분리하였고, 분리된 기관지 영상을 이용하여 중앙축 변환(MAT : .Medial Axis Transformation)을 통해 구해진 좌표값을 가상 카메라의 운행 경로로 사용하였다. 원근투영(perspective projection) 및 볼륨 데이터의 표면을 렌더링하기 위해 마칭큐브(marching cube) 알고리즘을 사용하였다. 기관지질환은 기관지 내부의 염증(inflammation) 이나 폐암(lung cancer)으로 기인하여 기관지 통로가 좁아지는 기관지 협착증, 기관지 확장증 및 기관지암으로 분류된다. 가상기관지경은 기관지 내부의 질환 위치와 질환 정도를 정성적으로 파악 할 수 있으며 기관지질환의 진단과 교육에 사용될 수 있다.

• 대한원격탐사학회지
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• 제23권5호
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• pp.409-420
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• 2007

Since 1988, pine wilt disease has spread over rapidly in Korea. It is not easy to detect the damaged pine trees by pine wilt disease from conventional remote sensing skills. Thus, many possibilities were investigated to detect the damaged pines using various kinds of remote sensing data including high spatial resolution satellite image of 2000/2003 IKONOS and 2005 QuickBird, aerial photos, and digital airborne data, too. Time series of B&W aerial photos at the scale of 1:6,000 were used to validate the results. A local maximum filtering was adapted to determine whether the damaged pines could be detected or not at the tree level from high resolution satellite images, and to locate the damaged trees. Several enhancement methods such as NDVI and image transformations were examined to find out the optimal detection method. Considering the mean crown radius of pine trees, local maximum filter with 3 pixels in radius was adapted to detect the damaged trees on IKONOS image. CIR images of 50 cm resolution were taken by PKNU-3(REDLAKE MS4000) sensor. The simulated CIR images with resolutions of 1 m, 2 m, and 4 m were generated to test the possibility of tree detection both in a stereo and a single mode. In conclusion, in order to detect the pine tree damaged by pine wilt disease at a tree level from satellite image, a spatial resolution might be less than 1 m in a single mode and/or 1 m in a stereo mode.

• 한국광학회지
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• 제20권1호
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• pp.53-56
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• 2009

타원법을 사용하여 인간 중간엽 줄기세포의 광학상수와 두께의 영상정보를 정량적으로 구하였다. 영상 타원계를 이용하여 in vitro 환경에서 세포의 핵(nucleus)과 세포체(cell body)의 타원상수 $\Delta$, $\Psi$를 2차원 영상정보 형태로 구한 다음 각 화소별로 타원상수들을 수치해석적 역방계산하여 굴절률과 두께의 영상정보를 얻었다. 세포의 핵 영역과 세포체 영역에서 줄기세포의 두께와 굴절률 값을 2차원 영상정보 형태로 제시하였다.