• Title/Summary/Keyword: open-chain

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Molecular Cloning and Nucleotide Sequence of Amaranthus viridis Homologue of the H -Transporting ATPase Gene (비름에서 ATP 가수분해효소와 상동성을 가지는 유전자의 클로닝)

  • 한규웅
    • Journal of Life Science
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    • v.6 no.1
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    • pp.1-5
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    • 1996
  • Using differential hybridization, a cDNA clone was isolated fortuitously from Amaranthus viridis and sequenced. This nucleotide sequence exhibited 55.1% identity with vma6 which encodes the 36-kD subunit of the vacuolar proton transporting ATPase in Saccharmoyces cerevisiae. The predicted open reading frame encodes a protein of 221 amino acid sequence with a calculated molecular weight of 25,452 and reveals high levels of similarity with subunit D polypeptide of vacuolar H -ATP(e.g., 48.5, 52.1 and 49.3% identity to the vacuolar 36-kD chain of yeast, vacuolar 32-kD polypeptide IV of human and vacuolar 28-kD protein of bovine chromaffin granules, respectively). The hydropathy index computation revealed that this predicted protein is a peripheral protein. These results indicated that the predicted protein may play a sturctural role in the vaculor H -ATPase as does gamma subunit in V-type ATPase.

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Redesign and Evaluation of a Chickpea Harvester

  • Golpira, H.
    • Journal of Biosystems Engineering
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    • v.40 no.2
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    • pp.102-109
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    • 2015
  • Purpose: Slow manual harvesting of rain-fed chickpeas cultivated in fallow fields in developing countries have encouraged the design of a mechanical harvester. Methods: A tractor-pulled harvester was built, in which a modified stripper header detached pods from an anchored plant and a chain conveyor transferred material. The stripper harvester was redesigned to use: 1) the maneuverability of tractor-mounted frames, 2) the adaptability of floating headers, and 3) the flexibility of pneumatic conveyors. Results: A mobile vacuum conveyor, which was an innovator open system, was designed for the dilute phase transferring mode for both grain and material other than grain. A centrifugal fan transferred harvested material to a cyclone separator that settled harvested material in a grain tank 1 m high. The machine at the spot work rate of $0.42ha{\cdot}h^{-1}$ harvested chickpea pods equal to the output of 16.6 farm laborers. Conclusion: The low cost and reasonable projected purchase price are the advantages of the concept. Additionally, the shattering loss reduction confirms the feasibility of the prototype chickpea harvester for commercialization.

Synthesis, Protonation Constants and Stability Constants for Transition Metal ions(II) of 1, 15-Bis(2-Hydroxybenzyl)-2, 6, 10, 14-Tetraazapentadecane (1, 15-Bis(2-Hydroxybenzyl)-2, 6, 10, 14-Tetraazapentadecane 리간드합성, 양성자 해리상수 및 전이금속에 대한 안정도 상수결정)

  • Kim, Sun-Deuk;Kim, Jun-Kwang;Lee, Woo-Sik
    • Analytical Science and Technology
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    • v.13 no.6
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    • pp.810-814
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    • 2000
  • The open-chain hexadentate $N_4$, $O_2$ ligands 1, 15-bis(2-hydroxybenzyl)-2, 6, 10, 14-tetraazapentadecane (BSATPD. 4HCl) has been synthesized as its tetrahydro-chloride salt and characterized by EA, IR, NMR, and Mass spectrum. Its protonation constants ($logK_n{^H}$) and stability constants ($logK_{ML}$) for $Cu^{2+}$, $Ni^{2+}$, $Co^{2+}$ and $Zn^{2+}$ ions were determined in aqueous solution by potentiometry and compared with those of analogous $N_4$, $O_2$ ligands contain ethtylenic spacers or propylenic spacers, which make six-membered chelate rings between the aliphatic nitrogen atoms.

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A study on the detection method of environment toxic gases by using electrical signal (전기적 신호처리에 의한 환경유해물질 검출연구)

  • Chon, Y.K.;Sun, J.H.;Lee, T.S.
    • Proceedings of the KIEE Conference
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    • 1999.07e
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    • pp.1997-2000
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    • 1999
  • 본 연구에서 제시하는 기공세라믹(Porous Ceramic)에 의한 누설전류 측정법은 기공 사이즈가 일정한 Open Pore Cell내에서 도전성 물질 및 이온화된 물질이 기공 사이에 침투되었을 때 외부에서 전계를 가하므로 써 이들 이온화 된 물질이 chain처럼 배열되어 전기적 병렬회로를 구성시켜 미세한 누설전류를 흐르게 한다. 이 누설전류법에 의한 도전성분 검출을 여러 환경 배출가스에 대한 모의실험을 실시한 결과 기공세라믹인 센서 자체의 누설전류는 가스 온도 150 ($^{\circ}C$) 이상에서 급격한 변화를 보이고 200($^{\circ}C$)에서 센서 자체의 전류치와 가스를 주입하였을 때의 전류치와는 상당한 격차를 두고 변화됨을 알 수 있었다. 그리고 공장연돌이나 자동차 배기관에서 방출되는 가스 중 HC, CO, NO, $CO_2$, $SO_2$, $N_2$ 등에 대한 센서 특성이 각각 달리 나타남을 알 수 있었다.

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Biomechanical for rehabilitation of the knee (슬관절 재활을 위한 생역학)

  • Kim Tae-Sook;Kim Sik-Hyun;HwangBo Gak
    • The Journal of Korean Physical Therapy
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    • v.12 no.2
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    • pp.239-247
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    • 2000
  • Knowledge of the anatomy and biomechanics of the knee is critical fur successful rehabilitation fellowing knee injury and surgery. Biomechanics of both the tibiofemoral and patellofemoral joints must be considered. The purpose of this paper is to provid framework for rehabilitation of the knee by reviewing the biomechanics of the tibiofemoral and patellofemoral joints. This will include discussion of the relevant arthrokinematics as well as the effects of open and closed chain exercises. The implications for rehabilitation of the knee will be highlighted.

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Cloning and Expression of a cDNA AAPT3 Encoding Aminoalcoholphosphotransferase Isoform from Chinese Cabbage

  • Kim, Kwang-Soo;Park, Jong-Ho;Cho, Sung-Ho
    • Animal cells and systems
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    • v.8 no.2
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    • pp.105-109
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    • 2004
  • Aminoalcoholphosphotransferase catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP-aminoalcohol such as CDP-choline or CDP-ethanolamine. Previously we suggested the presence of possible isoforms of this enzyme from Chinese cabbage roots and now report the cDNA cloning and expression analysis of AAPT3 encoding a third isoform of aminoalcoholphosphotransferase (AAPT3). AAPT3 contains an open reading frame of 1,176 bp coding for a protein of 392 amino acids. It shares 96 and 95% identity with Chinese cabbage AAPT1 and AAPT2, respectively, at the deduced amino acid level. The results from reverse transcriptase-polymerase chain reaction analysis indicate that expression of AAPT3 is up-regulated by low temperature as well as AAPT1 and AAPT2.

Isolation and Characterization of Cinnamoyl CoA Reductase Gene from Codonopsis lanceolata

  • Kim Ha-Na;In Jun-Gyo;Lee Eun-Kyung;Yoon Jae-Ho;Lee Mee-Sook;Bae Hyeun-Jong;Yang Deok-Chun
    • Plant Resources
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    • v.8 no.3
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    • pp.181-187
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    • 2005
  • A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.${\times}$aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.

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KT의 M2M 추진현황

  • Hwang, Yeong-Heon
    • Information and Communications Magazine
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    • v.27 no.7
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    • pp.10-15
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    • 2010
  • 그 동안 M2M 시장은 각 분야에서 IT를 융합하다가 자연발생적으로 형성되기 시작하면서, 복잡한 Supply Chain과 Long-Tail적 사업 특성을 가져 크게 활성화되지는 못하였다. 하지만 최근 무선기술의 발달, 글로벌 시장의 성장, 비용의 하락으로 인해 새로운 전기를 맞고 있다. 이에 따라 M2M 시장에 대해 KT는 M2M 인프라 구축과 제공, M2M 시장 창출과 활성화, 정부의 M2M 관련 사업에 대한 적극적인 참여 등을 기본방향으로 하여 추진 중이다. 특히 시장 창출과 활성화를 위해 저가 범용의 M2M 모듈을 개발하고, 요금상품을 재구성하는 증 비용 절감과 함께 M2M 플랫폼을 직접 구축, 외부 사업자에 제공하여 진입장벽을 낮추며, Open Ecosystem을 통해 관련 사업주체 간에 상호 Win-Win할 수 있는 사업환경을 만들어 나가고 있다. 본 원고는 종합 통신 사업자로서의 KT가 공공의 이익과 기업의 영리를 함께 고려하면서 M2M 사업을 진행해나가는 내용을 다루고 있다.

Isolation of the Phosphoribosyl Anthranilate Isomerase Gene (TRP1) from Starch-Utilizing Yeast Saccharomycopsis fibuligera

  • Park, Eun-Hee;Kim, Myoung-Dong
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1324-1327
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    • 2015
  • The nucleotide sequence of the TRP1 gene encoding phosphoribosyl anthranilate isomerase in yeast Saccharomycopsis fibuligera was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 759 bp, including the stop codon, encoding a 252 amino acid residue. The deduced amino acid sequence of Trp1 in S. fibuligera was 43.5% homologous to that of Komagataella pastoris. The cloned TRP1 gene (SfTRP1) complemented the trp1 mutation in Saccharomyces cerevisiae, suggesting that it encodes a functional TRP1 in S. fibuligera. A new auxotrophic marker to engineer starch-degrading yeast S. fibuligera is now available. The GenBank Accession No. for SfTRP1 is KR078268.

Heterogeneous Chain-mail Model for CPU-based Volume Deformation (CPU 기반의 볼륨 변형을 위한 다형질 Chainmail 모델)

  • Lee, Sein;Kye, Heewon
    • Journal of Korea Multimedia Society
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    • v.22 no.7
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    • pp.759-769
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    • 2019
  • Since a surgery simulation should be able to represent the internal structure of the human body, it is advantageous to adopt volume based techniques rather than polygon based techniques. However, the volume based techniques induce large computation to deform heterogeneous volume datasets such as bones and muscles. In this study, we propose a new method to deform volume data using multi-core CPUs. By improving previous studies, the proposed method minimizes unnecessary propagation operations. Moreover, we propose an efficient task-partitioning method for volume deformation using multi-core CPUs. As a result, we can simulate the deformation of heterogeneous volume data at an interactive speed without special hardware.