Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Cloning and Expression of a cDNA AAPT3 Encoding Aminoalcoholphosphotransferase Isoform from Chinese Cabbage

  • Kim, Kwang-Soo (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University) ;
  • Park, Jong-Ho (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University) ;
  • Cho, Sung-Ho (Department of Biological Sciences and Institute of Molecular Cell Biology, College of Sciences, Inha University)
  • Published : 2004.06.01
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Abstract

Aminoalcoholphosphotransferase catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP-aminoalcohol such as CDP-choline or CDP-ethanolamine. Previously we suggested the presence of possible isoforms of this enzyme from Chinese cabbage roots and now report the cDNA cloning and expression analysis of AAPT3 encoding a third isoform of aminoalcoholphosphotransferase (AAPT3). AAPT3 contains an open reading frame of 1,176 bp coding for a protein of 392 amino acids. It shares 96 and 95% identity with Chinese cabbage AAPT1 and AAPT2, respectively, at the deduced amino acid level. The results from reverse transcriptase-polymerase chain reaction analysis indicate that expression of AAPT3 is up-regulated by low temperature as well as AAPT1 and AAPT2.

Keywords

References

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