• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.510-515
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• 2006

The putative EPA synthesis gene cluster was mined from the entire genome sequence of Shewanella oneidensis MR-1. The gene cluster encodes a PKS-like pathway that consists of six open reading frames (ORFs): ORFSO1602 (multi-domain beta-ketoacyl synthase, KS-MAT-4ACPs-KR), ORFSO1600 (acyl transferase, AT), ORFSO1599 (multi-domain beta-ketoacyl synthase, KS-CLF-DH-DH), ORFSO1597 (enoyl reductase, ER), ORFSO1604 (phosphopentetheine transferase, PPT), and ORFSO1603 (transcriptional regulator). In order to prove involvement of the PKS-like machinery in EPA synthesis, a 20.195-kb DNA fragment containing the genes was amplified from S. oneidensis MR-1 by the long-PCR method. Its identity was confirmed by the methods of restriction enzyme site mapping and nested PCR of internal genes orfSO1597 and orfSO1604. The DNA fragment was cloned into Escherichia coli using cosmid vector SuperCos1 to form pCosEPA. Synthesis of EPA was observed in four E. coli clones harboring pCosEPA, of which the maximum yield was 0.689% of the total fatty acids in a clone designated 9704-23. The production yield of EPA in the E. coli clone was affected by cultivation temperature, showing maximum yield at $20^{\circ}C$ and no production at $30^{\circ}C$ or higher. In addition, production yield was inversely proportional to glucose concentration of the cultivation medium. From the above results, it was concluded that the PKS-like modules catalyze the synthesis of EPA. The synthetic process appears to be subject to regulatory mechanisms triggered by various environmental factors. This most likely occurs via the control of gene expression, protein stability, or enzyme activity.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2007년도 정기학술발표대회 논문집
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• pp.495-498
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• 2007

최근 분양가 상한제 시행 ${\cdot}$ 분양원가 공개 등으로 건설 사업비에 대한 사회각층의 관심이 고조되고 있으나, 사회기본시설에 대한 민간투자법 ${\cdot}$ 분양가 상한제 등에서 제시하고 있는 원가체계(구성항목)가 상이하여 혼선이 예상된다. 현재까지 원가관리 및 비용 산정 등에 관련된 많은 연구결과물이 제시되었으나, 지속적인 DB축적 불가 ${\cdot}$ 원가체계의 표준화 미비 ${\cdot}$ 활용방안 제시 미비 등으로 연구개발 이후 사장되고 있는 실정이다. 따라서 본 연구에서는 기 수행된 공사의 실적자료를 효율적으로 수집, 관리하고 건설생산 단계에서 활용할 수 있도록 양질의 정보 형태로 가공한 후 DB구축을 통하여 원가정보 활용방안 제시를 목적으로 한다. 궁극적으로는 발주자의 사업에 대한 이해와 설득을 통한 사업수주능력을 극대화하고, 차후 원가관리 업무에 유용한 도구개발의 자료로 활용할 수 있다.

• Human Ecology Research
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• 제55권2호
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• pp.167-180
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• 2017

This study makes suggestions on the contents of home economics to form adolescents' values of a healthy marriage and family. For this objective, family-related contents of high school home economics textbooks between Korea and the U.S. were comparatively analyzed using a qualitative interpretation method, based on the elements of a healthy family. Obtaining nine types of high school technology & home economics textbooks (2009 revised curriculum), two types of high school home economics science textbooks (2009 revised curriculum), and five types of the U.S. home economics textbooks for grade 10 to 12, contents elements, described contents, pictures, illustration, and activity tasks of the contents were examined. This study discussed the implications of family-related content in home economics high school textbooks of Korea as well as presented suggestions to be used as basic data for the development of subject content. The results of the study can be summarized as follows. In the results of analyzing the family-related contents in home economics textbooks of both countries, Korean home economics textbooks emphasized the contents meeting a democratic and gender-equal family relationship, maintenance & creation of healthy family life culture, rational resource management, and support for children's growth & development. However, U.S. home economics textbooks focused on elements such as economic stability of family, stable food, clothing, and shelter, open conversation, support of children's growth & development, rational resource management, sharing family roles, healthy citizenship, and harmony between work and family.

• Human Ecology Research
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• 제56권3호
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• pp.247-262
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• 2018

This study investigates the process of PCK formation for 2017 novice teachers in Busan. This study was conducted parallel to quantitative research using the H-PCK measurement tool as well as qualitative research methods through an open questionnaire and after-interview. Consequently, the H-PCK level of novice teachers was 3.51. The knowledge of perspective on home economics education (KP) was 3.87, knowledge of home economics curriculum (KC) was 3.37 and the knowledge of home economics instructional strategies (KI) was 3.39. Study participants reported that curriculum knowledge, curriculum content knowledge, and teaching strategy knowledge were formed through preparation for appointment; however, it was not possible to judge if PCK was formed for the restructuring ability or the reconstruction ability. Knowledge related to curriculum content was learned through teacher training, internet teacher community, and internet information materials. Knowledge of teaching strategies was learned through 1-2 teaching consulting or peer scholarship. It is necessary to revise the university curriculum (such as expansion of curriculum education, expansion of experience in teaching practice, and actual case study) order to form PCK. Second, it should be developed and operated various training programs for the formation of home economics education curriculum knowledge. Third, it is necessary to organize a nationwide system to support the mentor. In the future, it is necessary to study PCK analysis for each unit and topic as well as to explain methods that can be provided to field teachers.

• 한국실내디자인학회논문집
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• 제29호
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• pp.281-288
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• 2001

It shows 21st century as a networking society that is moving up the concept of global town and being industrialized with regional and personal connections. Individual's original idea and variety of 21th century may be respected by such environmental change, and age (Literary, human axis, technology axis, nature axis, kaining farming axis are central keyword) of culture that cultural capacity of individual becomes important fetters that create added value is forecasted to become. The moaning for space that is unemployment enemy by change of conceptional environment about cultural life and agreeable quality of life by development of Information-Communication technology may be required newly. As it does interaction along with development of science technique and Information-Communication technology, the future environment of various that complex! Is predicted to form network environment (existence space, cyberspace) newly. Human central interest is risen in technology balance hereupon, and these characteristic escaped in physical system that do with functional special quality and require human central and sensitive interaction. First, if examine about phenomenon by Digitize and chance aspect that is risen in 21th century, Digitize is time that action occurs fusion Tuesday that is various and Blur phenomenon of city·space, and the period to collapse the border between several individual. Second, importance more than man-centered and sensitive aspect of functional physical system is risen by digital age with development of technology medium, and as the five digital senses showed up, it suggests a sensual of the times and therefore the interest and direction get set up for the sensual sides of consumers. Third, special quality is, medium enemy by that digital space connotes meaning disk floret, variability, transparency, space red of extensity etc.. to burn and is digitalised, can be risen by symbolic, original individualities and emotional communication's the importance is required sensitivity enemy who is sympathy horn by fusion anger of individual. Technology of new media may open direction of new communication through interface that did not enjoy so far is going to supply new means that can express own to human

• 한국조경학회지
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• 제13권2호
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• pp.13-26
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• 1985

^x The enviroment defines the living conditions for people and has at the same time the possibility to create new environment. In Korea, where the rate of urbanization reached more than 50% in 1970′s the priority has been put on the economic development and administrative function. Under the circumstance, visual environmental field was dealt lightly and it resulted in undesirable environment. The techniques of Mordern Arts (Montage, Depeysment, Tromp L′oeil, P.O.P Art, etc.) helped Super Graphics appear in the urban areas. Environmental Art has been expended into the public space and people came to recognize the Arts as the "Street Art" or "Street as Gallery". Super Graphics has four types 1) Resident′s Super Graphics ; Minority groups came to maintain social equality and rights, in cooperation with each other. Such maintenance required general urbanites to form communities which gave birth to the community art, Mural Painting. 2) Environmental Super Graphics ; Beauty has come to be stressed in order to improve the quality of urban lives in the course of inescapable urban development. Instead of renewal of all established construction conservation oriented renewal was encouraged. 3) Super Graphics as Population Arts ; In the 1960′s artists repulsed the establishments in an efforts to open new phase independent from the expressional in the arts. They recognized the relationship between painting, society and the public in different angle and tried to describe all living space on canvas. 4) Super Graphics as Advertisement ; Super Graphics functions as efficient media to deliver images to the urbanites. Super Graphics as media plays the role for political propaganda and commercial advertisements according to their purposes. In Korea, especially, it is required to introduce the environmental Super Graphics. But it is desirable to introduce Super Graphics with Korean culture and sense of beauty. Designers themselves are also required to have responsibility to improve the quality of urban culture.

• 지능정보연구
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• 제9권3호
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• pp.193-205
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• 2003

본 논문에서는 인터넷 뱅킹의 사용자 인증에 있어 더 강인성(Robustness)을 갖춘 인증 시스템을 위해서 생체의 특징을 이용해 신분을 증명 또는 인증하는 생체인식 기술 중 지문이나 장문, 정맥, 홍채를 이용한 인식과 같이 장비에 접촉해야만 것과 달리 거부감이 없고, 별도의 전문 장비를 필요로 하지 않아 일반 대중들에 쉽게 접근할 수 있는 얼굴인식을 이용해 인증 시스템의 설계 및 구현을 제안한다. 얼굴인식 알고리즘은 얼굴 특징을 분석하는 방식에 따라 PCA (Principal Component Analysis), ICA (Independent Component Analysis), FDA (Fisher Discriminant Analysis) 등이 발표되어 있다. 이들 중 가장 기본적인 알고리즘이라 할 수 있는 PCA를 이용해 얼굴 특징을 분석하고 암호화된 형태의 생체 데이터를 전달해 분석한 결과를 원격지에 신속하고 정확하게 송수신할 수 있는 인터넷 뱅킹에서의 사용자 인증을 위한 얼굴인식 시스템의 설계 방법을 제안한다.

• 한국건설순환자원학회논문집
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• 제7권4호
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• pp.333-340
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• 2019

레드머드(Red mud)는 보크사이트(Bauxite)를 원료로 알루미나를 제조하는 공정에서 발생하는 산업부산물이다. 한국의 경우 베이어프로세스 공정을 통하여 Al2O3 1톤을 생산할 경우 함수율 50%의 레드머드가 약 2톤이 발생된다. 강알칼리성의 레드머드를 중화시킴으로서 재활용을 증대시킬 수 있고 보관 및 관리 측면에서 환경부하를 저감시킬 수 있다. 더욱이 재활용하기 위해 가열 및 분쇄 공정이 필요없는 액상화 레드머드에 대한 관심이 증대되고 있다. 따라서 본 논문에서는 질산 및 황산의 첨가량에 따른 액상화 레드머드의 pH 변화를 검토한 후 중화시킨 액상화 레드머드의 첨가량에 따른 시멘트 페이스트의 압축강도 특성을 비교 검토하였다. 그 결과 질산 및 황산으로 중화시킨 액상화 레드머드의 압축강도는 중화하지 않은 액상화 레드머드와 비교하여 상대적으로 높게 나타났다.

• 한국콘텐츠학회논문지
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• 제17권9호
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• pp.619-636
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• 2017

본 연구는 수퍼비전을 통한 수퍼바이저와 수퍼바이지의 관계형성에 대한 내러티브적 묘사이다. 즉, 수퍼바이저 A의 삶과 수퍼바이지 B의 삶 속에서 맺고 있는 그들의 관계를 있는 그대로 기술 및 설명하였다. 본 연구로부터 얻은 결론은 진솔성과 개방성은 인간중심 상담의 핵심요소이며, 특히 수파바이저의 무조건적인 수용에 대한 노력은 수직관계 형성으로 '은폐' 방법을 사용하기 쉬운 수퍼바이지의 태도를 더욱 개방적으로 유도할 수 있었다. 이것은 수퍼비전의 관계는 상하관계라는 기존의 시각에 새로운 관점을 제시한다. 수퍼비전에 초보 수퍼바이지들을 적극적으로 참여 할 수 있도록 수퍼바이저들이 먼저 수용하고 신뢰할 수 있는 분위기와 라포(rapport)를 형성한다면, 수퍼비전의 관계는 수직에서 수평으로 더욱 진전될 수 있음을 본 연구를 통하여 알 수 있었다.

• 한국전기전자재료학회논문지
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• 제26권3호
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• pp.246-251
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• 2013

Screen printing is commonly used to form the front/back electrodes in silicon solar cell. But it has caused high resistance and low aspect ratio, resulting in decreased conversion efficiency in solar cell. Recently the plating method has been combined with screen-printed c-Si solar cell to reduce the resistance and improve the aspect ratio. In this paper, we investigated the effect of light induced silver plating with screen-printed c-Si solar cells and compared their electrical properties. All wafers were textured, doped, and coated with anti-reflection layer. The metallization process was carried out with screen-printing, followed by co-fired. Then we performed light induced Ag plating by changing the plating time in the range of 20 sec~5min with/without external light. For comparison, we measured the light I-V characteristics and electrode width by optical microscope. During plating, silver ions fill the porous structure established in rapid silver particle sintering during co-firing step, which results in resistance decrease and efficiency improvement. The plating rate was increased in presence of light lamp, resulting in widening the electrode with and reducing the short-circuit current by shadowing loss. With the optimized plating condition, the conversion efficiency of solar cells was increased by 0.4% due to decreased series resistance. Finally we obtained the short-circuit current of 8.66 A, open-circuit voltage of 0.632 V, fill factor of 78.2%, and efficiency of 17.8% on a silicon solar cell.