• Journal of Aquaculture
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• v.9 no.2
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• pp.125-132
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• 1996

A study was carried out to test sex ratio of the progeny that were induced by the artificial fertilization between normal female and gynogenetic diploid eggs and sex-reversed gynogenetic diploid male sperm. Sex reversed gynogenetic diploid males were produced successfully in gynogenetic diploid female by immersion treatment with $17{\alpha}$-methyltestosterone at the concentration of 10 pub from $13.5{\pm}0.22$ mm to $57.6{\pm}5.1$ mm in total length. In $F_1$ progeny test, matings between normal females and sex-reversed gynogenetic males were not produced all female, but matings between gynogenetic diploid females and sex-reversed gynogenetic males were produced all female populations in six out of eight groups and two lots produced $96.9\%$ and $95\%$ female. These data showed that all-female populations were produced by crossing between gynogenetic diploid female and sex reversed gynogenetic diploid male.

• Journal of Aquaculture
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• v.9 no.4
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• pp.445-452
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• 1996

Endogeneous activities of ${\beta}-galactosidase$-like enzyme in various tissues from several finfishes and shellfishes were examined by histochemical analysis based on X-gal staining and by fluorimetric measurement using 4-methylumbelliferyl-${\beta}$-D-galactoside (4-MUG). Species used in this study were 3 freshwater fishes, mud loach (Misgurnus mizolepis), common carp (Cyprinus carpio) and tilapia (Oreochromis niloticus) ; 3 marine fishes, olive flounder (Paralichthys olivaceus), stone flounder (Kareius bicoloratus) and marbled sole (Limanda yokohamae) ; and 4 shellfishes, abalone (Haliotis discus hannai), Pacific oyster (Crassoskra gigas), pearl oyster (Pinctada fucata martensii) and ark shell (Anadara broughtonii). The activities of ${\beta}-galactosidase$-like enzyme in all finfishes examined were significantly different among species, with the wide variations between tissues in a species. In general, the tissues such as kidney, intestine and liver were ones which showed the significantly higher values in 4-MUG fluorimetry and deeper staining patterns in X-gal analysis compared to other tissues. On the other hand, serum and muscle revealed the significantly lower activities than others did, regardless of species. Shellfishes were also found to have endogenous activities of ${\beta}-galactosidase$-like enzyme which were significantly varied depending on both species and organs in a species. Hepatopancreas from all shellfishes examined showed the deepest pattern in X-gal staining and also the highest value in 4-MUG analysis, while activities of ${\beta}-galactosidase$-like enzyme in adductor muscles and mantle muscles from all shellfish species in this study except Pacific oyster were negligible : Pacific oyster had the significant activity of this enzyme in muscle tissues. Putative endogenous lacZ fragment was amplified from both finfishes and shellfishes by polymerase chain reaction (PCR). The molecular size of PCR products was about 510 bp, and there was no difference in size among species examined.

• Culinary science and hospitality research
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• v.19 no.4
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• pp.243-255
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• 2013

The purpose of this study is to examine the menu selection attributes in Italian restaurants located in Jeonju area and analyze their customers' using patterns and favorite menu items in each menu section. The results are as follows. First, in terms of significance of choosing a menu item in Italian restaurants, the most important factors are defined as health management factors, service management factors, food management factors, and menu management factors. After the verification of satisfaction, there are significant differences in the health management factors. Second, in terms of Italian restaurant visiting patterns, the recognition level of Italian food was positive, number of visits per month was averagely once or twice a month, major companions were family members, main purpose was to having meals, and information of the food was acquired by word of mouth. Third, in each menu section, the most popular dishes were the one using mozzarella cheese in the antipasto, asparagus cream soup in the soup, carbonara in the pasta, the pizza topped with mozzarella cheese, salami, black olive, and tomato sauce in the pizza, fresh mozzarella cheese tomato salad in the salad, beef tenderloin steak and pan-fried halibut in the main dish, fresh fruits and tiramisu in the dessert. For future studies, there should be various researches and analyses for significant factors when selecting menu by each segment and group from the entire sample.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.10
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• pp.1265-1273
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• 1999

PIV(Particle Image Velocimetry) velocity field measurements inside the snout of a1/10 scale model of the Zn plating process were carried out at the strip speed $V_s=1.5m/s$. Aluminum powder particles ($1{\mu}m$) and atomized olive oil ($3{\mu}m$) were used as seeding particles to simulate the molten Zinc flow and deoxidization gas flow, respectively. A pulsed Nd:Yag laser and a $2K{\times}2K$ high-resolution CCD camera were synchronized for the PIV velocity field measurement. From flow visualization study, it is found that the liquid flow in the Zn pot is dominantly governed by the uprising flow caused by the rotating sink roll, with its effect on the steel strip inside the snout largely diminished by installing of the snout. The deoxidization gas flow in front of the strip inside the snout can be characterized by a large-scale vortex rotating clockwise direction formed by the moving strip. In the rear side of the strip, a counter-clockwise vortex is formed and some of the flow entrained by the moving strip impinges on the free surface of molten zinc. The liquid flow in front of the strip is governed by the flow entering the snout, caused by the spinning sink roll. Just below the free surface a counter-clockwise vortex is formed near the snout wall. The moving strip affects dominantly the flow behind the strip inside the snout, and large amount of the liquid flow follows the moving strip toward the sink roll. The thickness of the flow following the strip is very thin in the front side due to the uprising flow, however thick boundary layer is formed in the rear side of the strip. Its thickness is increased as moving downstream toward the sink roll. Inside the snout, the deoxidization gas flow above the free surface is much faster than the liquid flow in the zinc pot. Due to the larger influx of the flow following the moving strip in the rear side of the strip, higher percentage of imperfection can be anticipated on the rear surface of the strip.

• Research in Plant Disease
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• v.22 no.1
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• pp.50-54
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• 2016

In early June 2014, leaf spot symptoms were observed on black chokeberry (Aronia melanocarpa) in Yesan-gun and Geumsan-gun, Chungcheongnam-do in Korea. The initial symptoms on leave surfaces were brown small-circular spots with a yellow halo lesion, and gradually the small spots were fused, all of infected leaves dropped eventually. A fungus were isolated from the initial lesion, and cultured on potato dextrose agar. Colony color on upper surface of plate varied from olive gray to charcoal gray. Size of conidia mostly extend to $19-50{\times}5-9{\mu}m$ in nature and $20-59{\times}8-13{\mu}m$ in culture, with 3-8 transverse septa and usually no longitudinal septum or only 1 longitudinal septum in 1-3 of the transverse compartments, and also have a short or long beak. Pathogenicity was investigated using wounded or unwounded black chokeberry and apple leaves. After 7 days of inoculation, leaf spots were similar to the symptoms naturally occurred in the field. On the basis of mycological characteristics, pathogenicity, and ITS rDNA sequence analysis, this fungus was identified as Alternaria mali. This is the first report of Alternaria leaf spot on black chokeberry caused by A. mali in Korea.

• 한국해양학회지
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• v.29 no.3
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• pp.217-227
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• 1994

From northeast to southwest, discontinuous sand ridges distribute on the western continental shelf of Korean Peninsular. The dimension of sand ridges is 3 to 21 m high, 3.1 to 6.8 km wavelength and 9-64 km long with 0.5 steep slope. they are probably originated and reformed by the intensity of tidal current according to the sea level rise. The characteristics of sand ridges revealed in study area are summarized as follows: (1) The sand ridges line up with the long axes of the tidal current ellipses, indicating a tidal control. (2) these are composed of two sedimentary sequences on the 3.5 kHz seismic profiles and core sediments. The upper sequence characterized by prolonged type is covered with thin veneer of massive fine sand(Mz, 2-3$\phi$) with Olive Gray(5Y 5/2). The lower sequence is characterized by internal reflector type with parallel and discontinuous. It consists of sandy mud or muddy sand(Mz, 5-7$\phi$) with laminar structures. the parallel internal reflectors are truncated on the slope of sand ridges. (3) Asymmetrical sand waves are superimposed on the sand ridges, and facing to the crest. However, symmetrical sand waves lie on the crest. Sand ridges having characteristics above is originated by scouring of tidal current, covered with coarase relict sediments, and modified by sadware.

• Korean Journal of Environmental Biology
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• v.37 no.2
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• pp.129-135
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• 2019

Juvenile Paralichthys olivaceus (mean weight 685.3±36.7 g) were raised in different and closed recirculating bio-floc system (control, bio-ball, and shelter) for 7 months. The water environment such as water temperature, dissolved oxygen, salinity, pH, and alkalinity according to the difference of closed recirculating system remained stable during the rearing period. No significant changes were observed in dissolved inorganic nitrogen such as ammonia, nitrite, and nitrate were observed in different closed recirculation system. The final weights according to the difference of closed recirculating were 1,524 g (control), 1,674 g (bio-ball), and 1,630 g (shelter). The survival rate was higher than 98%, and the final FCRs (Feed coefficient ratio) were 1.2, 1.1, and 1.2. The results of this study indicated high growth and survival rate in all systems.

• Research in Plant Disease
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• v.16 no.1
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• pp.74-80
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• 2010

Emulsification characters were investigated for COY (Cooking oil and Egg Yolk Mixture) and control efficacies of COY was enhanced by supplemented Sulfur wp. against paprika powdery mildews. Amount of water added to one egg yolk and 100 ml olive oil affected tbe homogenization of mixtures to emulsion profoundly; those premixes with 5~10 ml water were too concentrated to be homogenized, hence not dispersible in water; those with 15~20 ml water were homogenized, but tended to flocculation and not readily dispersible in water, suggesting the instability of emulsion; and those with 50 or 100 ml water were homogenized well and was readily dispersible in water to stable emulsion over long period of time. It was further confirmed that those fruits sprayed with not-fully emulsified COY revealed the oily membranes or blotches on their surfaces, whereas the ones with fully emulsified COY revealed normal clean surfaces. Treatment of COY either alone or in supplementation with Sulfur wettable powder (sulfur wp) were also effective in suppressing the recurrence of powdery mildew signs, wbich had disappeared in three days of foliar application. Still, the COYs supplemented with sulfur wp (COY+sulfur wp) at 1,000x or 5,000x were significantly effective in suppression against the symptomlsign recurrence over COY or sulfur wp alone.

• The Journal of Internal Korean Medicine
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• v.29 no.3
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• pp.730-741
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• 2008

Objective : To examine the efficacy of CY, the improvement of atopy dermatitis(AD)-like lesions on the rostral back of the NC/Nga mice, which took CY orally and were treated with a chemical substance called DNFB, the inhibition of ear swelling, and the inhibition of inflammatory response of the ear tissue of the mice were observed and compared, and the inhibition of the serum IgE count and the IL-4 and IFN-${\gamma}$ count produced by CD4+ T cells of the lymph node were observed and compared. Materials and Methods : For measurement of ear thicknesses, 0.15% DNFB $25{\mu}l$ mixed with acetone/olive oil(3:1) was applied to the right ear and dorsal skin of the NC/Nga mice 5 times at an interval of 7 days. Ear thickness was measured every day over a five-week period after the first application of DNFB. The total serum IgE count was measured with ELISA by taking blood samples 24 hours after the fifth application of DNFB. To measure the IL-4 and IFN-${\gamma}$ count, the lymph node was cut off, and the CD4+ T cells were purified and stimulated to activate the T cells, and then the IL-4 and IFN-${\gamma}$ count was measured with ELISA. Results : Continuous oral administration of CY improved the AD-like skin lesions on the rostral back and inhibited the ear swelling in NC/Nga mice treated with DNFB and inhibited the inflammatory response in the NC/Nga mice treated with DNFB NC/Nga mice. Continuous oral administration of CY did not significantly inhibit the serum IgE count and failed to significantly reduce the IL-4 count generated after TCR stimulation in the CD4+ T cells of the NC/Nga mice treated with DNFB. Continuous oral administration of CY significantly reduced the IFN-${\gamma}$ count generated after TCR stimulation in the CD4+ T cells of the NC/Nga mice treated with DNFB.

• Journal of fish pathology
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• v.18 no.2
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• pp.133-146
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• 2005

A bacteriological survey in maricultured fish farms was conducted in Korea from 2002 to 2004. A total number of 166 Vibrio isolates were collected from diseased fishes, including olive flounder (133 isolates), black rock fish (8 isolates), red sea bream (6 isolates), shrimp (5 isolates), black sea bream (4 isolates), abalone (3 isolates) and other fishes (7 isolates). All isolates were phenotypically characterized and then groups were obtained using the traditional biochemical test. Representative isolates of each group were genotypically characterized with sequencing the 16S rRNA genes or 16S-23S intergenic space genes. Above 14 species of Vibrio were identified as V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) and others Vibrio sp. (24 strains) by combining of biochemial and genetic characteristics.