• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.556-557
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• 2001

This study was performed to improve the efficency of production of biosudactant which were produced by newly screened MNNGCN-Methyl-N-Nitro- Nitrosoguanidine) mutagenized P. aeruginosa EMS1. A culture grown exponentially for $30^{\circ}C$ in trypic soy brotb is adjusted to pH. MNNG is added and incubated in water bath shaker at about 250 ${\sim}$300rpm. After 20 min, is dilutecl into colded trypic soy broth and centrifugation. The cell pellet is resuspended in 50$m{\ell}$ of trypic soy broth. Cultures are grown at $30^{\circ}C$ overnight. cetyltrimethylammonium bromide-metbylene blue agar plate selected dark blue halo colony. Peanut oil, Castor oil, Olive oil, and so on were compared as carbon source of surface tension and emulsifying activity.

• Journal of the Korean Home Economics Association
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• v.42 no.10 s.200
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• pp.105-113
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• 2004

This research was designed to investigate the preferred textiles for the jackets of college students. 105 subjects that majored in fashion design were surveyed. The subjects selected three favorite materials, according to the seasons, among 120 samples presented in a swatch book and their frequency of choice was tallied. The extent of the subjects' preference was compared by season and gender The results of this study were as follows: 1. College students preferred dark Grayish Brown plain variation wool Crossbred foremost as a textile for the spring and fan season. 2. White plain cotton Plisse was the most preferred material for summer. 3. Olive Gray cotton Corduroy was the most preferred textile for winter. 4. For thickness and weight, the textiles chosen for winter were thicker and heavier than those chosen for other seasons. 5. For fiber content, natural fiber such as wool were preferred for all seasons. 6. For the type of fabrics weave, a plain variation weave was preferred for spring and fall, twill was preferred for winter and plain weave was preferred for summer. 7. Collage students preferred gray and brown tones for the spring, fall and winter season. For color characteristics, solid fabrics were generally preferred and check patterns were especially preferred for the winter season. 8. Girl students generally preferred lighter and thinner fabrics than those chosen by boys.

• The Korean Journal of Nuclear Medicine
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• v.23 no.1
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• pp.63-69
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• 1989

In order to develop technetium-99m-labelled human albumin microsphere (HAM) for lung scintigraphy, various experimemts such as preparation and fractionation of HAM, establishment of optimal labelling conditions, determination of radiochemical purity, stability test and biodistribution of $^{99m}Tc-HAM$ were carried out. HAM was prepared from the suspension of 1ml aqueous human serum albumin (25%) in 130 ml of olive oil at $130\sim135^{\circ}$ with vigorous stirring. The resulting HAM was fractionated with microsieve to get the desired particle size $(15\sim50\mu)$ and autoclaved for sterilizaiton. The HAM particles were treated with stannous chloride and the pH of the suspension was adjusted to $3.0\sim3.5$ with phosphate buffer. After freeze-drying the contents of single reaction vial containing 5 mg of HAM and 0.2 mg of $SnCl_2$ it was reacted with $Na^{99m}TcO_4$. The labelling yield was higher than 99.5% and the stability of $^{99m}Tc-HAM$ was high enough to maintain 99.1% of radiochemical purity up to 24 hours. Lung and liver uptake in mice was found to be 94% and 0.9%, respectively. Excellent rabbit and human lung scans were also obtained.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.12 no.1
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• pp.7-12
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• 2006

The EU regulation of overall migration which is the cornerstone of the limitation to ensure that plastic materials and articles intended to contact with foodstuffs are fit for the intended application, was reviewed. In Council Directives, it was established in detail what simulants, contact times and temperatures are to be used in migration tests performed under standardized conditions. In some cases, conventional conditions for substitution test was provided, because of technical reasons. Moreover, a reduction factor ranging from 2$\sim$5 may be applied depending on the foods, because olive oil which is simulant for fatty foods, is severe solvent compared with most fatty food.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.200-200
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• 1996

간재생 및 만성 간질환모델에 대한 인진호 추출분획의 간장 약효검색 방법 1. 간세포 재생능력에 대한 효과 : SD계 수컷 흰쥐(체중 300-350g)를 마취하에서 복부정중선을 따라 개복후 간의 중앙엽 및 좌측엽을 절제하여 봉합하고 수술조작을 끝낸 추 4시간, 익일부터 7일간 오후 5시에 약물을 경구투여하고 마지막투여 후 20시간 절식, 부검하여 간재생률 및 BSP 정체율을 측정하였다. 2. $CCl_4$ 만성간장해 모델에 대한 효자 SD계 수컷 흰쥐(체중 200-250g)에 $CCl_4$ : olive oil 40%용액을 0.5 $m\ell$/kg b.wt. 용량으로 6일간 결구투여 한 후 7일째부터 매일 시험약물을 $CCl_4$ 혼합액 투여 4시간전 및 4시간후에 2회 경구투여하였으며 이 조작을 8일간 하고 마지막 투여 24시간 후에 부검하여 혈액내 ALT 및 AST치, 간조직내 total protein, triglyceride 및 hydroxyproline 양을 측정하였으며, 간중량도 측정하였다. 3. 약물투여 음성대조 : 1% CMC-Na 용액(10$m\ell$/kg b.wt.) 양성대조 : silymarin(25mg/kg), DDB(37.5mg/kg) 인진호추출분획 : KP(180mg/kg), PS-1(300mg/kg), PS-2(300mg/kg), EE (500mg/kg ), HH(640mg/kg), BE(1500mg/kg)

• Environmental Mutagens and Carcinogens
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• v.22 no.4
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• pp.324-330
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• 2002

Bisphenol A (BPA) is a monomer widely used in the manufacturing polycarbonate plastics or epoxy resin, and xenobiotics recently known as endocrine disrupting chemical. In this paper, to assess the effects of bisphenol A on immunopathological parameters (body weight, organ weight, hematological parameters, cellularity and surface marker) in mice, ICR female mice were orally exposed to BPA dissolved in olive oil as concentrations of 100, 500, 1000 ㎎/㎏/day b.w. 5 days a week for 30 days (subacute exposure). Liver - and kidney weight was significantly increased as dose-dependent manner, but body- , spleen- and thymus- weight didn't changed. In hematological parameters, WBC and MCHC were lowered but HCT and MCV were siginificantly enhanced. There was no significant differences in peritoneal macrophages number of the mice exposed to BPA. However, number of splenocytes of spleen, CD3/sup +/ and CD4/sup +/ cell in splenocytes, CD4/sup +/ and CD8/sup +/ cell in thymocytes were decreased at the mice subacutely exposed to BPA. In addition, BPA decreased expression of B7-1 and B7-2 on macrophages. Therefore, these results showed BPA may affect hematological parameters, cellularity and surface marker of immunocytes.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.193-199
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• 2010

An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the $sll0396{\Omega}$ mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin ${\beta}$- and ${\alpha}$-subunits and lacked the 30- and 32-kDa linker polypeptides, and northern blot analysis revealed that the transcription of the 1.4-kb cpcBA gene encoding the phycocyanin ${\beta}$- and ${\alpha}$-subunits was lower in the mutant. The Sll0396 protein has a DNA-binding motif and shares homology with known response regulators. Our results indicate that Sll0396 plays a regulatory role in the transcription of the phycocyanin genes during phycobilisome synthesis.

• Journal of fish pathology
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• v.33 no.1
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• pp.91-95
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• 2020

Fish bacterial diseases have spread and caused serious problem for cultured marine fish in Korea. The important bacterial disease affecting mariculture such as olive flounder (Paralichthys olivaceus) are caused by Edwardsiella tarda, Vibrio scophthalmi and Streptococcus parauberis. For the bacterial disease protection in aquaculture industry, the water treatment is needed in aquaculture system. During the last decades atmospheric pressure non-thermal plasma in contact with liquids have received a lot of attention of environmental and medical application. In this study, we determined the disinfectant effect in seawater and three major fish bacterial disease pathogens by using low frequency plasma treatment. Three fish bacteria (E. tarda, V. schophthalmi, S. parauberis) were not detected within 16 min, 150 min and 270 min of 20 L, 500 L and 1 ton seawater post low frequency plasma treatment, respectively. Three major fish bacterial disease pathogens were not detected within 2 min after the low frequency plasma treatment, suggesting that the low frequency plasma possess disinfectant effectiveness.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.555-564
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• 2019

Biodiesel is produced through the transesterification process in the presence of alcohol and a catalyst that catalyzes the conversion of triglycerides to esters and glycerol compounds. A more optimal product conversion can be achieved using enzymes, such as lipase. Lipase is reported to be produced in osmophilic yeasts due to the low water content in their natural habitats. Wild forest honey is one of the osmophilic natural habitats in Indonesia. However, lipase-producing yeast has not been reported in the Indonesian honey. In this study, we screened the lipase-producing yeasts isolated from wild forest honey collected from Central Sulawesi. The production profile and activity of lipase were determined at different pH values and temperatures. One promising yeast was isolated from the honey, which was identified as Zygosaccharomyces mellis SG 1.2 based on ITS sequence. The maximum lipase production (24.56 ± 1.30 U/mg biomass) was achieved by culturing the strain in a medium containing 2% olive oil as a carbon source at pH 7 and 30℃ for 40 h. The optimum pH and temperature for lipase activity were 6 and 55℃, respectively. The enzyme maintained 80% of its activity upon incubation at 25℃ for 4 h. However, the enzyme activity decreased by more than 50% upon incubation at 35 and 40℃ for 2 h. This is the first study to report the lipase producing capability of Z. mellis. Further studies are needed to optimize the enzyme production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.4
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• pp.431-434
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• 2014

Cytogenetic analysis was conducted to obtain basic information for chromosome manipulation of starry flounder Platichthys stellatus. Nuclear surface area and volume of erythrocyte were $7.60{\pm}0.93{\mu}m^2$ and $12.80{\pm}1.75{\mu}m^3$, respectively. The haploid DNA content of the species was 0.66 pg/haploid cell which correspond to 93% of olive flounder Paralichthys olivaceus. A karyotype analysis was also carried out with the species using conventional staining and Ag-NOR banding techniques. It was consisted of 48 acrocentric chromosomes and inter-sex or intra-individual polymorphism was not detected in all specimens analyzed. The NOR regions, appearing a terminal position of the short arm of the smallest acrocentric pairs.