• Korean Journal of Soil Science and Fertilizer
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• v.42 no.5
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• pp.355-363
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• 2009

Double cropping system of oilseed crops introduced rape and castor bean was studied in the newly reclaimed tidal land of Korea. Surface soil EC to reach at 50% of growth reduction to the tallest height of crops was estimated $4dS\;m^{-1}$ for castor bean and $6dS\;m^{-1}$ for rape by logarithmic function. The castor bean cultivated during rainy summer much more suffered serious growth and yield reduction by flooding damage in the Hwaong and Yeonsangang reclaimed lands having low soil conductivity and finer textured soil than in the Iweon reclaimed land having higher soil conductivity and coarse sandy textured soil. Rape cultivated during dry winter-spring much more suffered serious growth and yield reduction by high soil salinity come from re-salting process. 50% yield reduction was estimated at $2.0\;2.5dS\;m^{-1}$ of surface soil EC by logarithmic function. Consequentially, it was concluded that oilseed production for energy by double cropping system of rape and castor bean might be possible under good controling soil salinity below $4dS\;m^{-1}$ for castor and $3dS\;m^{-1}$ for rape in the newly reclaimed land of Korea.

• BMB Reports
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• v.40 no.2
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• pp.247-260
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• 2007

Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.227-234
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• 2012

We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiation-related genes were highly expressed at 10 DAF, whereas energy-related genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.371-379
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• 2010

Crimson clover (Trifolium incarnatum), hairy vetch (Vicia villosa) and rye (Secale cereale) are common leguminous cover crops. Because they contain water-soluble allelopathic substances that show a variable level in tissue depending on growth stage of the plants, the weed inhibition effects are dependent on the growth stage of cover crops. This study investigated the allelopathic effects of crimson clover, hairy vetch and rye on soybean, radish, oilseed rape and lettuce seeds germination and seedling growth. We used extract that were prepared from the shoots of crimson clover, hairy vetch and rye at different growth stages, vegetative growth stage, flowering stage, and fruiting stage. Applications of aqueous extracts from three growth stage of cover crops strongly affected to oilseed rape and lettuce seeds germination but the treatment resulted in a slight inhibition of the germination in soybean and radish. Radical length was more sensitive to aqueous extracts than seed germination rate. Especially, three cover crops extracts at vegetative growth stage highly inhibited seedling root growth of oilseed rape and lettuce by over 80% and 90% respectively. Furthermore, the $GR_{50}$ values were lowest in the treatment of extracts from vegetative growth stage and the level of phenolics was decreased by the order of vegetative growth stage, flowering stage and fruiting stage.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.277-283
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• 2010

Rape (Brassica napus L.) plants are one of the major oilseed crops. The main components of rapeseed are oil (35 to 47%) and protein (15 to 32%). For the biodiesel production, the development of a new variety of rape plant with high biomass and/or oleic acid contents is required. In order to determine the optimum dose of gamma-ray irradiation, the rape seeds of cvs. Hanra (Hr), Youngsan (Ys), Tammi (Tm), and Tamra (Tr) were irradiated with a 100~4,000 Gy dose range of gamma-rays. Considering the growth factors, the optimum doses were determined to be within the range of 600~1,000 Gy for the selection of useful mutant lines. Six-hundred and eighty-eight (688) $M_2$ mutant lines were obtained from 600~1,000 Gy gamma-ray-irradiated $M_1$ plants through selfing. The growth characteristics, leaf shape, early flowering, and flower color were all investigated. The selected mutant numbers of early flowering, leaf shape, and flower color were 34, 52, and 3 from the four cultivars, respectively. These mutant lines will be used for the development of a new variety of rape plant with high biomass and oleic acid contents.

• Journal of Biosystems Engineering
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• v.43 no.4
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• pp.311-319
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• 2018

Purpose: The aim of this study is to develop a small combine for harvesting miscellaneous cereal crops. Methods: A prototype small combine was designed and constructed. Its specifications and basic performance were investigated. Results: The prototype small combine for harvesting miscellaneous cereal crops was designed and constructed to reflect similar specifications as those of the conventional combine. The prototype small combine comprises a diesel engine with the rated power/speed of 22.0 kW/2,600 rpm, three-stage primary and two-stage speed range transmission shifts, and a double acting threshing part. The maximum travel speeds of the prototype combine are approximately 0.72 m/s, 2.50 m/s, 0.30 m/s at the low, high speed range shifts in the forward direction, and while traversing in the reverse direction, respectively. The minimum radius of turning was approximately 1.50 m. In a static lateral overturning test, the prototype combine overturned neither to the right nor to left on a $30^{\circ}$ slope. The results of an oilseed rape harvesting test included the maximum operating speed of 0.32 m/s, the grain loss ratio of approximately 9.0%, and the effective field capacity of approximately 10.3 a/h. Additionally, among the outputs in grain outlet, the whole grains, damage grains, and materials other than grain (MOG) ratios accounted for 97.4%, 0.0%, and 2.6%, respectively. Conclusions: The prototype small combine for harvesting miscellaneous cereal crops indicates good driving ability and stability. The results of the oilseed rape harvesting test reveal that the harvesting performance must be enhanced such that the separating and cleaning parts are more suitable for each type of crop, thus reducing grain loss and foreign substances among the outputs in grain outlet. An improved small prototype combine could be used effectively to mechanize the harvesting of miscellaneous cereal crops in small family farms or semi-mountainous areas.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.2
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• pp.75-80
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• 2019

Oilseed rape is known to crop having low nitrogen use efficiency (NUE) but requires high levels of N fertilizer. NUE is associated with N remobilization from source to sink organ, consequently affects seed yield. Remobilization of leaf N is also related to transport of C/N metabolites in phloem. However, interaction between seed yield and phloem transport was not fully documented. In response to seed yield, N and C metabolites and their transport into seed from bolting to pod filling stage investigated in two contrasting genotypes (Capitol and Pollen) cultivated under ample (HN) or limiting nitrate (LN) supply. Seed yield was significantly reduced in N limitation and its reduction rate was much lower in Capitol than in Pollen compared to HN treated plants. Amino acid and protein content was higher in Capitol than in Pollen at bolting stage. They gradually decreased during plant development but not significant between two cultivars and/or two treatments. Glucose, fructose and sucrose content were 1.8-,1.6- or 1.25-fold higher in LN condition than in HN condition, respectively. Amino acid and sucrose content in phloem were largely higher in Capitol than in Pollen under LN condition. These results indicate that the higher seed yield might be related to greater transport ability of amino acid and sucrose in phloem under LN condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.2
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• pp.109-115
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• 2023

Ammonium (NH₄⁺) serves as a nitrogen source, but its elevated levels can hinder plant growth and production. Excess NH₄⁺ with α-ketoglutarate is assimilated into glutamate, a precursor of proline and glutathione (GSH). This study aimed to investigate the effects of excessive NH₄⁺ on the regulation of proline and GSH synthesis. Detached leaves from oilseed rape (Brassica napus L.) were fed with 0, 50, 100, 500, and 1000 mM NH₄Cl for 16 h. As the NH₄⁺ concentrations increased, the leaves exhibited progressive wilting and yellowing. Furthermore, total carotenoid and chlorophyll concentrations declined in response to all NH₄⁺ treatments, with the lowest levels observed in 1000 mM NH₄⁺ treatment. Hydrogen peroxide (H₂O₂) concentration showed a minor increase at low NH₄⁺ concentration (50 and 100 mM) treatments but a significant increase at high NH₄⁺ (500 and 1000 mM), which was consistent with the localization of H₂O₂. Amino acid concentrations increased with increasing in NH₄⁺ concentration, while the protein concentration displayed the opposite trend. Proline and cysteine concentrations exhibited a gradual increase in response to increasing NH₄⁺ concentrations. However, GSH concentrations rose only in the 50 mM NH₄⁺ treatment and decreased in the 500 and 1000 mM NH₄⁺ treatments. These results indicate that excessive NH₄⁺ is primarily assimilated into proline, while GSH synthesis is adversely affected.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.72-79
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• 2021

The increasing use of deicing salts has caused various environmental problems, including crop damage along the motorway where deicing salts are sprayed during winter. Deicing salts used on roads have been reported to negatively affect crops, but little information is known about their impact on crops. A seedling emergence assay was conducted to evaluate the effects of deicing salts on crops using oilseed rape (Brassica napus) as a model plant. We tested five chloride deicing salts consisting of NaCl, CaCl2, or MgCl2 and 1 non-chloride deicing salt (SM-3) at a range of concentrations (25, 50, 100, 200, and 400 mM), and untreated control. Regardless of deicing salts, they significantly delayed and reduced seedling emergence of oilseed rape with increasing salt concentration. Non-linear regression analysis of seedling emergence with a range of salt concentrations by fitting to the log-logistic model revealed that the chloride deicing salts reduced seedling emergence more than the non-chloride deicing salt SM-3. The GR50 value, the concentration causing 50% seedling emergence, of SM-3 was 47.1 mM, while those of the chloride deicing salts ranged from 30.7 mM (PC-10) to 37.5 mM (ES-1), showing approximately 10 mM difference between non-chloride and chloride deicing salts. Our findings suggest that seedling emergence assay is a useful tool to estimate the potential damage caused by deicing salts on crops.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.2
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• pp.66-70
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• 1998

Four introduced forage rape (Brassica napus) cultivars, 'Akela', 'Ramon', 'Sparta', and 'Velox' and a leading rapeseed cultivar, 'Hallayuchae' were grown at nitrogen (N) rates of 0, 100, 250, 300, 350, and 400 kg/ha to (i) select forage rape cultivars adapted best to Cheju area, and (ii) determine the optimum N rate for the best cultivars. Days from seeding to flowering across the cultivars increased 190 to 195 days as N rate increased from 0 to 400 kg/ha. Average days to flowering of six cultivars ranged from 182 to 198 days. Plant height increased as N rate increased up to 300 kg/ha and then decreased with a further increase in N rate and ranged from 159 to 174 cm among the cultivars. The optimum N rate for the greatest dry matter yield of five cultivars ranged from 222 to 258 kg/ha. Sparta showed the greatest dry matter yield (35.79 Mg/ha), followed by Akela, Hallayuchae, Velox, and Ramon. As N rate increased, crude protein content linearly increased but crude fiber content declined curvilinearly. Akela and Sparta had higher protein content than the other cultivars did. The forage cultivars had lower crude fiber content than the oilseed cultivar Hallayuchae did. Our results demonstrated that Sparta was best adapted to Cheju area and the optimum N rate for Sparta was about 220kg/ha.