• 한국환경과학회지
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• 제12권2호
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• pp.119-124
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• 2003

An effective grafting method for jujube nursery trees was developed to shorten the operation time, improve the percentage of "takes" and shoot emergence, and form the better graft union. Out of 7 grafting methods the splice, modified scion Ono graft and the bark graft showed relatively short operation time during the grafting operation comparing to the modified rootstock Ono graft or the chip budding. Among these methods, the bark graft demonstrated 100% "takes", but the modified scion Ono graft or the standard Ono graft showed 58.3% of "takes". The percentages of the vegetative shoot emergence were 100% fir the bark graft, 70% f3r the modified scion Ono graft and lower emergence percent for the rest grafting methods. The union tissue formation for the modified scion Ono graft, the bark graft, or the whip-and-tongue graft was significantly larger than the standard Ono graft or the splice graft. In a close examination of the cross sectional areas of the graft union formation through the microscope, the bark graft was superior, the standard Ono graft was inferior and rest of the grafting methods were intermediate.ing methods were intermediate.

• 농업과학연구
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• 제4권1호
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• pp.94-104
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• 1977

수도재배(水滔栽培)에 기계이앙(機械移秧)을 실시(實施)하기 위(爲)하여 조생통일외(早生統一外) 3개(固) 품종(品種)을 4월(月) 16일(日)부터 10일(日) 간격(間隔)으로 3회(回) 상자육묘(箱子育苗)하고 40일묘(日苗)를 이앙기(移秧機)로 이양(移秧)하였던 바 묘(苗)의 생육(生育)과 기계이앙후본답(機械移秧後本畓)에서의 수도(水滔)의 생육(生育)과 수량(收量)을 조사(調査)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 40일묘(日苗)의 초장(草長)과 엽수(葉數)는 관행육묘(慣行育苗)에 비(比)해 상자육묘구(箱子育苗區)에서 현저(顯著)히 짧은 경향(傾向)이었으며 상자육묘(箱子育苗)의 경우 물못자리 상자육묘구(箱子育苗區)의 초장(草長) 및 엽수(葉數)가 밭못자리구(區)보다 현저(顯著)히 길었다. 2. 40일묘(日苗)를 단근(斷根)하여 발근력(發根力)을 시험(試驗)한 결과(結果) 상자육묘구(箱子育苗區)의 발근력(發根力)은 관행육묘구(관行育苗區)의 발근력(發根力)에 비(比)해 현저(顯著)히 낮았다. 한편 같은 상자육묘구(箱子育苗區)에서는 물못자리 상자육묘구(箱子育苗區)의 묘(苗)의 발근력(發根力)이 밭못자리에 비(比)해 높은 경향(傾向)이었다. 3. 본시험(本試驗)에 공시(供試)된 유일계(流一系) 품종(品種)은 상자육묘(箱子育苗)의 경우 못자리에서 25~30일(日) 경(頃) 부터 잎의 상부가 황변(黃變) 내지 황적색(黃赤色)으로 변(變)하고 생육(生育)이 거의 정지(停止)되는 현상(現像)을 나타내었다. 4. 상자육묘(箱子育苗)의 기계이앙구(機械移秧區)는 관행육묘이앙구(慣行育苗移秧區)에 비(比)해 활착(活着)이 5~7일(日) 이상(以上) 지연되었으며 출수기(出穗期)도 품종(品種)에 따라 차이(差異)가 있으나 대체로 5~7일(日) 지연되었다. 5. 정조수량(正粗收量)은 유신(維新)의 관행재배구(慣行栽培區)에 비(比)해 모두 약간의 감수(減收)를 보였는데 기계이앙구내(機械移秧區內)에서는 5월(月) 26일(日) 이앙(移秧)의 경우 통일(統一)의 수량(收量)이 가장 높았고 6월(月) 15일(日)의 수량(收量)은 밀양(密陽) 15호(號)가 가장 많았으며 기계이앙재배(機械移秧栽培)를 위(爲)한 품종(品種)의 선택(選擇)은 중요(重要)한 것으로 인정(認定)된다. 6. 수도이앙기(水滔移秧機)로는 $3.3m^2$당(當) 이앙주수(移秧株數)를 73주(株)부터 108주(株)까지 조정(調整)이 가능(可能)하며 파종밀도(播種密度)가 균일(均一)하면 결주율(缺株率) 1% 미만(未滿)의 작업정도(作業精度)로 인력이앙작업(人力移秧作業)에 비(比)하여 2조(條)의 경우 5~6배(倍)의 이앙작업능률(移秧作業能率)을 올릴 수 있으므로 단작지대(單作地帶)에서는 실용화(實用化)가 용역(容易)하리라 믿는다. 7. 이모작지대(二毛作地帶)에서는 이앙시기(移秧時期)가 한정(限定)되어 있으므로 적절(適切)한 품종(品種)의 선택(選擇)과 상자묘(箱子苗)의 육묘기술(育苗技術)이 개발(開發)된다면 수량(收量)은 감소(減少)없이 이앙작업(移秧作業)의 기계화(機械化)가 무난(無難) 할 것으로 전망(展望)된다.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.269-276
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• 2008

Field resistance is defined as the resistance that allows effective control of a parasite under natural field condition and is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative trait loci (QTLs) conferring the resistance for races and blast nursery screening in japonica rice cultivars were detected and mapped using SSR markers. QTL analysis was carried out in 190 RILs population from the cross between Suweon365 (moderately resistant) and Chucheong (highly susceptible). Twelve QTLs against nine blast races inoculated were detected on chromosomes 1, 2, 4, 6, 7, 11 and 12. They explained from 5.1% to 34.9% of total phenotypic variation. Eight QTLs against blast nursery screening in four regions for three years were detected on chromosomes 1, 2, 4, 11 and 12. The phenotypic variation explained by each QTL ranged from 4.3% to 37.7%. Three chromosome segment substitution lines (CSSLs) of $BC_2F_6$ by backcross method were developed to transfer the QTLs into the susceptible cultivar Chucheong as a recurrent parent. A CSSL4-1 containing two QTLs qLB6.2 and qLB7 against blast races showed to the reaction of 6 to 7 at blast nursery in two regions for two years. The CSSL4-2 and CSSL93 containing QTLs, qLB11.2 and qLB12.1 of the resistance against leaf blast in blast nursery screening, respectively, had enhanced the resistance for blast nursery screening across two regions and in two years.

• Journal of Forest and Environmental Science
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• 제38권4호
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• pp.249-255
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• 2022

The study was conducted to establish a method for the proliferation of hybrid poplar (P. alba × P. glandulosa) clone Bongwha1, an excellent biomass species. It was found that to collect the cuttings of Bonghwa1, it was necessary to use the main stem rather than the axillary branch. Stem growth by green-wood cuttings showed a tendency to decrease as the length of the collected cuttings increased, but the survival rate was low. Therefore, modified leafy stem cutting was attempted to increase the survival rate of the cuttings. In the modified leafy stem cutting method, 4 leaves were included in the cuttings, and especially, cuttings were performed using cuttings containing 2-4 cm xylem parts. Leafy stem cutting increased root growth and the number of stems, as well as the survival rate of hybrid poplar clone Bongwha1 compared to green-wood cuttings. The root growth of the leafy stem cutting poplar was better as there was more xylem part. Using two-year-old nursery stocks, the leafy stem cutting was used to produce about 66 cuttings. This study is expected to contribute to the mass propagation of high-quality nursery stocks.

• 한국작물학회지
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• 제28권3호
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• pp.386-389
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• 1983

참깨 이식방법의 확립을 위하여 육묘이식방법에 대한 일연의 시험을 실시하여 얻은 결과는 다음과 같다. 1. 참깨솎음묘의 이식은 묘의 측근발생이 적을 뿐만 아니라 솎음할 때 뿌리가 절단되어 활착이 잘 안되고 생육이 부진하여 활용성이 없었다. 2. 참깨육묘이식은 이식시 뿌리의 절단이 적은 육묘방법(종이 pot, 비닐 pot, 연상 및 개량상)으로 했을 때 활착은 문제되지 않았으나 묘의 생육이 개량상 육묘 즉, 비닐위에 상토를 3cm로 하여 4방 5cm로 파종함으로 지상부 생육이 왕성하고 측근발생도 많을 뿐 아니라 노력도 절감되었다. 3. 6월 10일 파종 6월 30일 이식은 6월 15일 직파에 비해 개화기가 4일이상 빨랐으며 비닐 pot육묘나 개량상육묘는 주당삭수도 많아 수량이 비닐 pot 육묘가 10% 개량상육묘는 18% 증수하였다. 4. 참깨육묘에 적합한 상토는 흙, 모래, 퇴비를 1:1:1의 객적비로 하는 것이었다. 5. 상토의 깊이는 3cm정도로 하고 묘대시비량은 질소, 인산, 칼리를 4-2-4g/$m^2$로 하는 것이 적합하였다.

• 한국작물학회지
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• 제33권1호
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• pp.54-58
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• 1988

인삼종자의 자연상태에서의 배 생장 및 및 발아능력을 구명하기 위하여 파종직후 미숙배 상태의 종자를 묘상에 파종하였던 바 그 결과를 요약하면 다음과 같다. 1. 파종후 80일에는 관행개갑종자와 미숙배 종자직파구의 배율간에 유의차가 없었고 100일후에는 관행개갑종자에 비해 미숙배 종자직파구의 배율이 현저히 증가되었다. 2. 미숙배 종자 직파구의 파종 100일후 종자 자연개갑율은 관행개갑구 종자의 개갑율과 유의차가 인정되지 않았다. 3. 미숙배 종자직파구의 2개년 평균 발아율은 약 57%로서 관행개갑종자 추파구의 종자발아율과 유의차를 나타내지 않았다. 4. 발아 전엽후 묘삼의 경장, 경직경, 엽장 및 엽폭 모두 관행개갑종자 추파구와 미숙배 종자직파구간에 유의차가 인정되지 않았다.

• 한국약용작물학회지
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• 제20권5호
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• pp.365-371
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• 2012

This study was carried out to know the characteristics of flowering and bearing fruit, the optimum period, regions and methods for seed harvesting, the optimum temperatures for seed storage and germination, and the optimum period for sowing at nursery bed and seedling transplanting of Valeriana fauriei Briquet. The flowering and bearing fruit of Valeriana fauriei was developed from the before-year root. Optimum period for seed harvest of Valeriana fauriei was from late July to middle August, and optimum areas were the high elevated areas over 500 m above the sea level as Jinbu-myeon, Pyeongchang-gun, Gangwon-do. Using of net-bag for seed harvesting was the effective method to gather the full ripe seed, and bagging of net-bag was necessary from the season of middle May that was the flowering middle-stage. Germination rates don't show the difference among the different temperatures of storage as approximately 41% at $-20^{\circ}C$, $2^{\circ}C$ or $20^{\circ}C$ of seed storage temperatures. The optimum temperature range was in $15{\sim}30^{\circ}C$ for seed germination at nursery bed. The optimum period for seed sowing at nursery bed was the late February, and the optimum period for seedling transplanting was the middle April.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.52-52
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• 2018

Apple (Malus domestica) is one of the most economically important fruits in Korea. But virus infection has decreased sustainable production of apple and caused the serious problems such as yield loss and poor fruit quality. Virus or viroid infection including Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV) and Apple scar skin viroid (ASSVd) has been also reported in Korea, furthermore, its damages and economic losses have increased constantly. In our research, we tried to survey virus infection for commercial nursery trees of major apple cultivars, especially dwarfing rootstocks 'M.9' and 'M.26' as well as scions. Trees were collected from 11 locations which have produced a great amount of apple nursery stocks in Korea. Infection degree was investigated in apple cultivars, 'Hongro' and 'Fuji' using RT-PCR method. In the scion of cultivar 'Hongro', infection ratio of ACLSV, ASPV and ASGV were 100%, 81.8% and 100% respectively. In the rootstock of cultivar 'Hongro', infection ratio of ACLSV, ASPV, ASGV and ApMV were 90.9%, 81.8%, 100% and 9.1% respectively. In the scion of cultivar 'Fuji', infection ratio of ACLSV, ASPV and ASGV were 81.8%, 90.9% and 100% respectively. In the rootstock of cultivar 'Fuji', infection ratio of ACLSV, ASPV, ASGV and ApMV were 81.8%, 90.9%, 100% and 9.1% respectively. Infection of ASSVd was not detected in both cultivars. From our results, it was found that most of apple rootstocks and scions had multiple infections by apple viruses which have caused economic damage in fruit production.

• 한국약용작물학회지
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• 제25권4호
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• pp.238-243
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• 2017

Background: The production method of ginseng seedlings for ginseng cultivation is very important to ensure healthy rooting system as well as high quality, and yield of the resultant plants. This study was carried out to compare the growth characteristics of 2-year-old ginseng plants that were produced from seedlings grown in self soil nursery (SSN), nursery soil (NS) or hydroponic culture (HC). Methods and Results: The shading prop used was composed of four-layered 4 polyethylene (blue 3 + black 1) shade screen. The management of main field was done by inserting oil cake (1,200 kg/10 a) and then allowing Sudan grass to grow for a year. Seedling transplantation was carried out on April 6. Root growth was measured on October 25. Root weight was observed to be excellent at 6.0 g, following SSN transplantation. Root length was 21.2 cm for HC seedlings, but these plants had a physiological disorder (i.e., rusty root), in 83.5% plants of this treatment. The ratio of PD/PT (protopanaxadiol saponins / protopanaxatriol saponins) was higher in NS seedlings. Plant analysis revealed that Fe content was lower in HC seedlings with high rustiness. The growth of 2-years-old ginseng was different following these varying seedling cultivation methods, but seedlings from NS were not different from those grown in SSN. Conclusions: For the propagation of 2-year-old ginseng plants, NS seedlings may be a good substitute for SSN seedlings.

• 한국양봉학회지
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• 제33권4호
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• pp.251-260
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• 2018

Instrumental insemination of honey bee is an attractive alternative to natural mating in breeding program as it allows mating crosses between desirable queen and specific drone. However, nursery condition that the queen is kept before and after insemination is major factor affected to the performance of instrumentally inseminated queen. In this study, we evaluated the influences of three different nursery-conditions of push-in cages, mini nuclei and normal colonies on number of spermatozoa stored in the spermatheca, body weight, onset of ovipositon and performance of instrumentally inseminated Apis cerana queen. Our results demonstrated that instrumentally inseminated queens kept in mini nuclei and in normal hives showed no significant difference in queen's weight (159.8 and 166.2mg, respectively), number of spermatozoa in spermatheca ($2.02{\times}10^6$ and $2.76{\times}10^6$, respectively), proportion of queen supersedure (33.3 and 66.7% queen survival at 11 months after oviposition, respectively) and brood production, compared to naturally mated queens. In contrast, instrumentally inseminated queens kept in push-in cages showed significant difference of those above data in comparison to queens mated naturally. Our results suggested that instrumentally inseminated queens could be kept in mini nuclei containing about 1.000 attendant bees to have desirable performance of queen whereas the push-in method should be practiced for the purpose of using queen in the length of time less than 7 months.