Do, Thi Huyen;Dao, Trong Khoa;Nguyen, Khanh Hoang Viet;Le, Ngoc Giang;Nguyen, Thi Mai Phuong;Le, Tung Lam;Phung, Thu Nguyet;Straalen, Nico M. van;Roelofs, Dick;Truong, Nam Hai
Asian-Australasian Journal of Animal Sciences
/
v.31
no.5
/
pp.738-747
/
2018
Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials. Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information non-redundant sequence database. Here the BLASTX hits were further processed by the Metagenome Analyzer program to statistically analyze the abundance of taxa. Results: Microbial community in the rumen is defined by dominance of Bacteroidetes compared to Firmicutes. The ratio of Firmicutes versus Bacteroidetes was 0.36:1. An abundance of Synergistetes was uniquely identified in the goat microbiome may be formed by host genotype. With regard to bacterial lignocellulose degraders, the ratio of lignocellulolytic genes affiliated with Firmicutes compared to the genes linked to Bacteroidetes was 0.11:1, in which the genes encoding putative hemicellulases, carbohydrate esterases, polysaccharide lyases originated from Bacteroidetes were 14 to 20 times higher than from Firmicutes. Firmicutes seem to possess more cellulose hydrolysis capacity showing a Firmicutes/Bacteroidetes ratio of 0.35:1. Analysis of lignocellulolytic potential degraders shows that four species belonged to Bacteroidetes phylum, while two species belonged to Firmicutes phylum harbouring at least 12 different catalytic domains for all lignocellulose pretreatment, cellulose, as well as hemicellulose saccharification. Conclusion: Based on these findings, we speculate that increasing the members of Bacteroidetes to keep a low ratio of Firmicutes versus Bacteroidetes in goat rumen has resulted most likely in an increased lignocellulose digestion.
Present studies were carried out to get a basic information for biological control of soybean rod borer, Grapholitha glycinivorella Matsumura, causing main injury in soybean cultivation in Korea. 1474 native strains were cultivated in field to evaluate the pod borer damage. Pubescence density and color, maturity group, seed coat color, aphid and general leaf damage and seed damage by pod borer were investigated, and determined among their relationships. As another basic study for ecological control of insect, 6 leading varieties were planted on different planting date with 3 levels of plant spacing, on which pod borer damage and some related agronomic characters were studied. The results obtained one summarized as follows: Average injury of soybean pod borer was $5.2\%$ ranging $0-38\%$ in 1474 strains planted on 20 May. Non-significant correlations were found between pod borer damage and pubescence density and color, while pod borer damage closely related with maturity groupand aphid damage at $5\%$ level ofttests. Most severe damage was found in green seed coast color and maturity group V. Late and sparse plantings tend to have more damages to the pod borer than those of early and dense plantings. Pod setting date and period of pod maturing seemed related with pod borer damage. Bongeui and Chungbuk-baik cultivars were resistance to soybean pod borer, while Clark and Kumkang-daerip were susceptible in field tests.
The main objective of this study is to analyze problems of the Country of Origin Labeling(COOL) in small live fish(SLF) trade by applying the asymmetric information theory which is called ${\ulcorner}$Lemon Market Theory${\lrcorner}$. The purpose of importing SLF is to enhance fishing household income and import substitution effect as well. SLF importation is quite different in its nature from importing general fish and fish products for final/direct consumption. SLF are imported for the purpose of domestic aquaculture and naturalization where domestic production/supply of SLF is less than domestic demand for or such species are not native to Korean waters. Information asymmetric problems arise even in SLF trade as the same way in general goods and services. However, the information asymmetry issues in SLF trade are much more complex than non-living goods because SLF are traded in the live state. To alleviate such problems the Korean government initiated the general COOL scheme to imported SLF. However, many experts argues that such policy on SLF would not be appropriate because of SLF's very nature. Applying the lemon market theory, we can analyze how information discovery schemes are able to signal correct information to SLF trading parties and to result in more symmetric information in SLF trade markets. This research carried out a case study about small live eel(SLF) trade and its farming. The results showed that applying the COOL to small live eels just right upon coming into fish farms tends to increase substantially fish farmer's income and at the same time to lower unnecessary transaction costs. In particular, such transaction costs by imposing simply the general COOL on SLF may easily outweigh its benefits. For instance, to resolve the problems, the Korean ministry of agriculture and forestry(KMAF) has developed a quite different COOL rules from the general ones and has applied them to imported live cattle and medium/small livestocks. The KMAF's differentiated COOL policy on some imported livestocks may be a good case which can be applied to imported SLF. In addition to the differentiated COOL on SLF, Sanitary and Phytosanitary (SPS) and traceability system would play crucial complementary roles in alleviating information asymmetry problems in SLF trade. Advanced fisheries tend to strengthen their SPS system rather than to adopt the general comprehensive COOL schemes into imported SLF trades and domestic market exchanges.
In March 2016, the computer Go program, AlphaGo, defeated Sedol Lee, a Korean professional Go player of 9-dan rank. This victory by AlphaGo shows the rise in popularity of artificial intelligence (AI). Not only was this game a testament to machine performance, it was the type of game that extended the Turing test. When the interrogator cannot differentiate between human being and machine, the machine has passed the test. This article examines the interactions between AI and human beings and studies the social nature of intelligence through the AlphaGo case. Collins insists that knowledge or intelligence is social and embodied, and the interrogators in the Turing test can identify the difference between native members and non-members through their knowledge only. Applying this concept, AlphaGo, as subject A of this test, fulfilled its role of stirring up the classical "truth of human." Meanwhile, Lee as subject B, played to speak the truth by revealing his own qualities. Here, it is also important role that interrogators judge what it is. Many spectators, as interrogators, have intervened to confirm the border between human beings and machines by using their embodied and social knowledge.
The purpose of this study was to approach to the internal meanings included in the Happening and the culture of hippies, by analysing the basic mental conditions in the process of the Happening. And this study was composed of the concept and the development of Happening, the characteristics of the hippies that related in the midst of the happening's background, and the formativeness between these factors and hippies'fashion. Since the happening a genre of fine arts expression attended the New School for social research in New York in 1954, Allan Kaprow direct-influenced by John Cage used the word 'Hapening'first, practicing '18 Happenings in 6 parts'at the Rueben gallery in 1959. Kaprow's 18 Happenings was one of the earliest opportunities for a wider public to attend the live events that several artists had performed more privately for various friends. Despite the very different sensibilities and structures of artist's works, artists were all thrown together by the press under the general heading of 'Happening', following Kaprow's 18 Happenings. Being considered as the root of the Happening 'Expression of Sound'of John Cage was the discovery of the exisiting thing- the Happening. Most artists were to be deeply influenced by Cage's theories and attitudes-that is, his sympathy for Zen Buddihism and oriental philosophy-and by reports of the Black Mountain events. These events would directly reflect contemporary painting and stemmed from the Futurists, Dadaists and Surrealists. And Happening's development background was based on the culture of hippies. Swinging London had been under the sway of psychedelic drugs and utopian visions of 'hippie'wave sweeping in from Califonia. This wave, which affected solid middle-class youth first and formost, began in Haight Ashbury in San Francisco. Without dwelling on the hippie movement here, it is worth nothing that it resulted from the convergence of several undercurrents : consciousness-expending drugs, the anti-Vietnam war developments, the impact of English pop groups on American music and the rise of protest songs, and finally the beatnik tradition of non-conformism. Hippie culture and its pursuit of love, peace and psychedelia was the antitheses of 1960s main street fashion. The media gave everyone with long hair the label of 'hippie', but it was always a very loose collage of attitudes and styles. The rejection of sexual taboos was conveyed by the hippie's refusal to wear. Although the bold exposure of body raised controversies because it went against the existing moral values, it has a significant implications. Psychedelics brought mind-expansion and the possibillity that modern technology (light show, synthesized electronic sounds), new fabrics or colors, and LSD could be utilized to provide an escape route from the dreariness of modern life. During the 1960s, traditional costumes, many of which had never been seen outside their native regions, became sought after and adopted in the West, initially by the young, who wanted to demonstrate their solidarity with cultures uncontaminated by mass industry. The most ardent proponents of such folk costumes were the hippies. Hippies dress was sometimes decribed as 'anti-fashion', produced by a patchwork of ragged cast-offs and flamboyant accessories, of outmoded Western dress and time-honored ethnic garments all combined, modified and permutated into variety of personal statements. 'Flower Power'became a reality. From the results of this study, we can see the expanding trend of the influence and the concept of the sew art genre 'Happening'in the formativeness as well as the fine arts field.
In order to investigate the degree of grain shedding of red rices collected in Korea, breaking tensile and breaking bending strengths of 269 varieties; 82 Korean and 100 foreign red rices and 87 Korean cultivars including 26 native varieties, 30 Japonica-Indica hybrids and 31 Japonica type varieties, were measured at harvest time. Also, histological characteristics of abscission region between spikelet and pedicel were observed. The breaking tensile and breaking bending strengths of both Korean and foreign red rices were weaker than those of Japonica-Indica hybrids which were known as easily shedding varieties in Korea. Abscission layers were observed in the majority of Korean red rices except for 5 varieties, and those of all varieties belonging to both long grain types and 'Sare's were cracked completely at harvest time, while those of round grain types were not cracked except one variety. The breaking tensile and breaking bending strengths of the tested varieties having cracked abscission layer were weaker than those of non-cracked ones. Both strengths of breaking tensile and breaking bending were positively correlated with diameter of supporting zone and thickness of sclerenchyma tissue surrounding central vascular tissue of suporting zone at 0.1% significant level, respectively.
This study was performed in order to simplify the operation and minimize stress of donor and be readily available in the field with low cost and high quality embryos using the Direct Embryo Collection (DEC). Donors, at random stages of the estrous cycle, received a CIDR. 7 days later, 200 mg FSH was treated with 40, 30, 20, 10 mg FSH levels in declining doses twice daily by intramuscular injection for 4 days. On the 3rd day administration of FSH, 25 mg $PGF_2{\alpha}$ was administered and CIDR was withdrawn. After FSH injections were complete, donors were artificially inseminated twice at 12 hr intervals. The donor cattle received 250 ${\mu}g$ GnRH at time of 1 st insemination and embryos were recovered 8 days after the 1st insemination. Embryo collection from superovulated donors was performed to flushing by non-surgical methods of 3-way, 2-way and DEC (l-way). The average number of recovered embryos were 11.25${\pm}$0.63, 12.5${\pm}$0.65 and 11.75${\pm}$0.48 from operations of 3-way, 2-way and DEC methods, respectively. There were no significant differences among the embryo collection methods. Also, The average number of transferable embryos were 6.25${\pm}$0.48, 7.25${\pm}$0.48 and 7.25${\pm}$0.63 from each embryo collection procedures. The number of transferable embryos was no differences among the 3-way, 2-way and DEC methods, respectively. Meanwhile, the ratio of transferable embryos for all recovered embryos from DEC methods was higher as 61.7 % than 55.6 %, 58 % from methods of 3-way, 2-way. And the flushing solution required for recovering embryos by DEC method was significantly lower as 0.28${\pm}$0.32 1 than 1.8${\pm}$0.12 1, 1.75${\pm}$0.10 1 from 3-way, 2-way methods (p<0.05). Also, the time required for recovering embryos by DEC methods was significantly lower as 27${\pm}$2 min than 51${\pm}$3, 45${\pm}$2 min, respectively (p<0.05). In conclusion, these results suggest that DEC method for embryo collection may be effectively used for production of in vivo embryos using less flushing solution and, it might be effectively available in the field compared to conventional embryo recovery methods using 3-way or 2-way balloon catheter.
Kim, Seok-Joong;Jin, Jae-Soon;Kim, Dong-Man;Kim, Kil-Hwan
Korean Journal of Food Science and Technology
/
v.24
no.3
/
pp.193-198
/
1992
The inhibitory effects of radish juice on the mutagenicities of cigarette smoke condensate (CSC), methanol extract of charred part of fried or broiled saury pike (MECS) and 2-aminofluorene (2-AF) were examined by the use of Ames assay toward Salmonella typhimurium TA 98 strain. Radish juice exhibited inhibition percentage of about 100, 100 and 87 on the mutagenicities of CSC, two kinds of MECS and 2-AF, respectively. Except for juices of cabbage and leek, radish juice has inhibited more effectively the mutagenicity of CSC than other fruit or vegetable juices studied. Inhibitory effect of radish juice might be originated from the components with molecular weight above 50,000 and decreased sharply in 5 min by heat treatment at $100^{\circ}C$, but hardly changed at low and moderate storage temperatures such as $4^{\circ}C,\;10^{\circ}C,\;25^{\circ}C\;and\;35^{\circ}C$ for about 2 weeks. Precipitate obtained from ammonium sulfate saturation from 30 to 80% had inhibitory effect on the mutagenicity of CSC. Extracts from 3 bands of non-denaturing gel of $30{\sim}80%$ amnonium sulfate precipitate have exhibited the inhibitory effects on the mutagenicity of CSC.
Journal of the Korean Association of Oral and Maxillofacial Surgeons
/
v.34
no.5
/
pp.509-517
/
2008
DNA damage accumulates in cells as a result of exposure to exogenous agents such as benzopyrene, cigarette smoke, ultraviolet light, X-ray, and endogenous chemicals including reactive oxygen species produced from normal metabolic byproducts. DNA damage can also occur during aberrant DNA processing reactions such as DNA replication, recombination, and repair. The major of DNA damage affects the primary structure of the double helix; that is, the bases are chemically modified. These modification can disrupt the molecules'regular helical structure by introducing non-native chemical bonds or bulky adducts that do not fit in the standard double helix. DNA repair genes and proteins scan the global genome to detect and remove DNA damage and damage to single nucleotides. Direct reversal of DNA damage, base excision repair, double strand break. DNA repair are known relevant DNA repair mechanisms. Four different mechanisms are distinguished within excision repair: direct reversal, base excision repair, nucleotide excision repair, and mismatch repair. Genetic variation in DNA repair genes can modulate DNA repair capacity and alter cancer risk. The instability of a cell to properly regulate its proliferation in the presence of DNA damage increase risk of gene mutation and carcinogenesis. This article aimed to review mechanism of excision repair and to understand the relationship between genetic variation of excision repair genes and head and neck cancer.
Kim Chan-Soo;Song Gwan-Pil;Moon Myong-Ok;Kang Young-Jae;Byun Gwang-Ok;Kim Moon-Hong
Korean Journal of Agricultural and Forest Meteorology
/
v.7
no.3
/
pp.201-210
/
2005
This study was conducted to prepare efficient and systematic measures for nature conservation and management in Beomseom Islet, and to provide for basic data to investigate the process of vegetation change in the future. The vegetation of Beomseom Islet was classified to six vegetation type; i.e., Miscanthus sinensis community, Pinus thunbergii community, Iythophyte vegetation, evergreen broad leaf community, shrub community, and Pseudosasa japonica community. The size of each vegetation type was 40,230 $m^2$ ($23.3\%$) for shrub community, 39,366 $m^2$($22.8\%$) for Iythophyte vegetation, 30,012 $m^2$ ($17.4\%$) fur Pinus thunbergii community, 29,853 $m^2$ ($17.3\%$) for Miscanthus sinensis community, 5,564 $m^2$ ($3.2\%$) for evergreen broad leaf community, and 3,325 $m^2$ ($1.9\%$) for Pseudosasa japonica community. The area of non-vegetated sea cliff Bone that composed of bare rocks is 24,246 $m^2$($14.1\%$). We estimated that these distribution patterns of vegetation were the result of various environmental factors such as the steepness of slope and shallowness of soil as well as the cultivation of exotic plants causing disruption of native vegetation.
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