• Title/Summary/Keyword: non-classical

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Proteomic Analysis to Identify Tightly-Bound Cell Wall Protein in Rice Calli

  • Cho, Won Kyong;Hyun, Tae Kyung;Kumar, Dhinesh;Rim, Yeonggil;Chen, Xiong Yan;Jo, Yeonhwa;Kim, Suwha;Lee, Keun Woo;Park, Zee-Yong;Lucas, William J.;Kim, Jae-Yean
    • Molecules and Cells
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    • v.38 no.8
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    • pp.685-696
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    • 2015
  • Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.

Color comparison between non-vital and vital teeth

  • Greta, Delia Cristina;Colosi, Horatiu Alexandru;Gasparik, Cristina;Dudea, Diana
    • The Journal of Advanced Prosthodontics
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    • v.10 no.3
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    • pp.218-226
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    • 2018
  • PURPOSE. The aim of this study was to define a color space of non-vital teeth and to compare it with the color space of matched vital teeth, recorded in the same patients. MATERIALS AND METHODS. In a group of 218 patients, with the age range from 17 to 70, the middle third of the buccal surface of 359 devitalized teeth was measured using a clinical spectrophotometer (Vita Easyshade Advance). Lightness ($L^*$), chromatic parameters ($a^*$, $b^*$), chroma ($C^*$), hue angle (h) and the closest Vita shade in Classical and 3D Master codifications were recorded. For each patient, the same data were recorded in a vital reference tooth. The measurements were performed by the same operator with the same spectrophotometer, using a standardized protocol for color evaluation. RESULTS. The color coordinates of non-vital teeth varied as follows: lightness $L^*$: 52.83-92.93, $C^*$: 8.23-58.90, h: 51.20-101.53, $a^*$: -2.53-24.80, $b^*$: 8.10-53.43. For the reference vital teeth, the ranges of color parameters were: $L^*$: 60.90-97.16, $C^*$: 8.43-39.23, h: 75.30-101.13, $a^*$: -2.36-9.60, $b^*$: 8.36-39.23. The color differences between vital and non-vital teeth depended on tooth group, but not on patient age. CONCLUSION. Non-vital teeth had a wider color space than vital ones. Non-vital teeth were darker (decreased lightness), more saturated (increased chroma), and with an increased range of the hue interval. An increased tendency towards positive values on the $a^*$ and $b^*$ axes suggested redder and yellower non-vital teeth compared to vital ones.

Generalized complex mode superposition approach for non-classically damped systems

  • Chen, Huating;Liu, Yanhui;Tan, Ping
    • Structural Engineering and Mechanics
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    • v.73 no.3
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    • pp.271-286
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    • 2020
  • Passive control technologies are commonly used in several areas to suppress structural vibrations by the addition of supplementary damping, and some modal damping may be heavy beyond critical damping even for regular structures with energy dissipation devices. The design of passive control structures is typically based on (complex) mode superposition approaches. However, the conventional mode superposition approach is predominantly applied to cases of under-critical damping. Moreover, when any modal damping ratio is equal or close to 1.0, the system becomes defective, i.e., a complete set of eigenvectors cannot be obtained such that some well-known algorithms for the quadratic eigenvalue problem are invalid. In this paper, a generalized complex mode superposition method that is suitable for under-critical, critical and over-critical damping is proposed and expressed in a unified form for structural displacement, velocity and acceleration responses. In the new method, the conventional algorithm for the eigenvalue problem is still valid, even though the system becomes defective due to critical modal damping. Based on the modal truncation error analysis, modal corrected methods for displacement and acceleration responses are developed to approximately consider the contribution of the truncated higher modes. Finally, the implementation of the proposed methods is presented through two numerical examples, and the effectiveness is investigated. The results also show that over-critically damped modes have a significant impact on structural responses. This study is a development of the original complex mode superposition method and can be applied well to dynamic analyses of non-classically damped systems.

Experimental Study on Structural Behavior of Tapered non-compact Frame with Snug-tightened Conditions (밀착조임 볼트체결에 따른 판폭두께비가 큰 변단면 프레임의 구조성능에 관한 실험적 연구)

  • Chung, Kyung-Soo;Jeon, Bae-Ho;Park, Man-Woo;Do, Byung-Ho
    • Journal of Korean Society of Steel Construction
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    • v.24 no.3
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    • pp.257-265
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    • 2012
  • Current trends in steel construction include using tapered, non-compact sections to minimize the use of excess material as much as possible by choosing the cross-sections instead of the classical approach of using prismatic members. In addition, snug conditions, especially the end-plate type, have the advantage of incurring less construction costs and shorter assembly times as opposed to full pre-tensioned conditions. On the other hand, it is important to predict the collapse of the PEB system due to over-loading. Large-scale tests of tapered steel portal frames with non-compact sections were conducted. The primary test parameters included the bolt connection method and the loading condition (vertical and horizontal load). The test results on initial stiffness and load capacity were investigated. Furthermore, comparisons between the analytical and experimental data for load-displacement curves were initiated. In addition, we evaluated the applicability of a snug bolt for the PEB frame in the field.

Development and validation of a non-linear k-ε model for flow over a full-scale building

  • Wright, N.G.;Easom, G.J.;Hoxey, R.J.
    • Wind and Structures
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    • v.4 no.3
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    • pp.177-196
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    • 2001
  • At present the most popular turbulence models used for engineering solutions to flow problems are the $k-{\varepsilon}$ and Reynolds stress models. The shortcoming of these models based on the isotropic eddy viscosity concept and Reynolds averaging in flow fields of the type found in the field of Wind Engineering are well documented. In view of these shortcomings this paper presents the implementation of a non-linear model and its evaluation for flow around a building. Tests were undertaken using the classical bluff body shape, a surface mounted cube, with orientations both normal and skewed at $45^{\circ}$ to the incident wind. Full-scale investigations have been undertaken at the Silsoe Research Institute with a 6 m surface mounted cube and a fetch of roughness height equal to 0.01 m. All tests were originally undertaken for a number of turbulence models including the standard, RNG and MMK $k-{\varepsilon}$ models and the differential stress model. The sensitivity of the CFD results to a number of solver parameters was tested. The accuracy of the turbulence model used was deduced by comparison to the full-scale predicted roof and wake recirculation zone lengths. Mean values of the predicted pressure coefficients were used to further validate the turbulence models. Preliminary comparisons have also been made with available published experimental and large eddy simulation data. Initial investigations suggested that a suitable turbulence model should be able to model the anisotropy of turbulent flow such as the Reynolds stress model whilst maintaining the ease of use and computational stability of the two equations models. Therefore development work concentrated on non-linear quadratic and cubic expansions of the Boussinesq eddy viscosity assumption. Comparisons of these with models based on an isotropic assumption are presented along with comparisons with measured data.

Evaluation of Buckling Strength of Non-structured Plates by Using the Deformation Energy (변형에너지에 기반한 비정형 판부재의 좌굴강도 평가기법)

  • Zi, Goangseup;Kim, Hong-hyun;Ahn, Jin-young;Oh, Min-Han
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.3
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    • pp.102-113
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    • 2017
  • A new index for the buckling strength of non-structured plates is proposed. The external work or the deformation energy caused by the external loads or the boundary displacement controled by a load parameter is calculated along an equilibrium path of the member under consideration. If the second variation of the energy with respect to the parameter loses its positiveness, it defined as the limit of the stability. In contrast to the current method given in codes where the stability limit is evaluated by using only representative internal forces, the evaluation of the stability limit is always consistent even with the change of the distribution of the internal forces on the boundary. If the elasticity is concerned, the result from this proposed approach becomes identical to that from the classical methods.

Research on optimization of traffic flow control at intersections (교차로 교통 흐름 제어 최적화에 관한 연구)

  • Li, Qiutan;Song, Jeong-Young
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.3
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    • pp.15-24
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    • 2022
  • At present, there are few studies on signal control of pedestrian traffic flow and non-motor traffic flow at intersections. Research on the optimization scheme of mixed traffic flow signal control can coordinate and control the overall traffic flow of pedestrians, non-motor vehicles and motor vehicles, which is of great significance to improve the congestion at intersections. For the traffic optimization of intersections, this paper starts from two aspects: channelization optimization and phase design, and reduces the number of conflict points at intersections from spatial and temporal right-of-way allocation respectively. Taking the classical signal timing method as the theoretical basis, and aiming at ensuring the safety and time benefit of traffic travelers, a channelization optimization and signal control scheme of the intersection is proposed. The channelization and phase design methods of intersections with motor vehicles, non-motor vehicles and pedestrians as objects are discussed, and measures to improve the channelization optimization of intersections are proposed. A multi-objective optimization model of intersection signal control was established, and the model was solved based on NSGA-II algorithm.

An Operations and Management Framework for The Integrated Software Defined Network Environment (소프트웨어 정의 네트워크 통합 운영 및 관리 프레임워크)

  • Kim, Dongkyun;Gil, Joon-Min
    • Journal of Digital Contents Society
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    • v.14 no.4
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    • pp.557-564
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    • 2013
  • An important research challenge about the traditional Internet environment is to enable open networking architecture on which end users are able to innovate the Internet based on the technologies of network programmability, virtualization, and federation. The SDN (Software Defined Network) technology that includes OpenFlow protocol specifications, is suggested as a major driver for the open networking architecture, and is closely coupled with the classical Internet (non-SDN). Therefore, it is very important to keep the integrated SDN and non-SDN network infrastructure reliable from the view point of network operators and engineers. Under this background, this paper proposes an operations and management framework for the combined software defined network environment across not only a single-domain network, but also multi-domain networks. The suggested framework is designed to allow SDN controllers and DvNOC systems to interact with each other to achieve sustainable end-to-end user-oriented SDN and non-SDN integrated network environment. Plus, the proposed scheme is designed to apply enhanced functionalities on DvNOC to support four major network failure scenarios over the combined network infrastructure, mainly derived from SDN controllers, SDN devices, and the connected network paths.

A Preliminary Study of Near Real-time Precision Satellite Orbit Determination (준 실시간 정밀 위성궤도결정을 위한 이론적 고찰)

  • Bae, Tae-Suk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.27 no.1
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    • pp.693-700
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    • 2009
  • For real-time precise GPS data processing such as a long baseline network RTK (Real-Time Kinematic) survey, PPP (Precise Point Positioning) and monitoring of ionospheric/tropospheric delays, it is necessary to guarantee accuracy comparable to IGS (International GNSS Service) precise orbit with no latency. As a preliminary study for determining near real-time satellite orbits, the general procedures of satellite orbit determination, especially the dynamic approach, were studied. In addition, the transformation between terrestrial and inertial reference frames was tested to integrate acceleration. The IAU 1976/1980 precession/nutation model showed a consistency of 0.05 mas with IAU 2000A model. Since the IAU 2000A model has a large number of nutation components, it took more time to compute the transformation matrix. The classical method with IAU 2000A model was two times faster than the NRO (non-rotating origin) approach, while there is no practical difference between two transformation matrices.

Vibration and Stability Analysis of a Multi-stepped Shaft System of Turbo Compressor (터보 압축기 다단 회전축계의 진동 및 안정성 연구)

  • Seo, Jung-Seok;Kang, Sung-Hwan;Park, Sang-Yoon;An, Chang-Gi;Song, Ohseop
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.24 no.8
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    • pp.583-591
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    • 2014
  • The mathematical modeling on the free vibration and stability of a multi-stepped shaft of turbo compressor is performed in this study. The multi-stepped shaft is modeled as a non-uniform Timoshenko beam supported by anisotropic bearings. It is assumed that the shaft is spinning with constant speed about its longitudinal axis and subjected to a conservative axial force induced by front and rear impellers attached to the shaft. The structural model incorporates non-classical features such as transverse shear and rotary inertia. A structural coupling between vertical and lateral motions is induced by Coriolis acceleration terms. The governing equations are derived via Hamilton's variational principle and the equations are transformed to the standard form of an eigenvalue problem. The implications of combined gyroscopic effect, conservative axial force, bearing stiffness and damping are revealed and a number of pertinent conclusions are outlined. In this study analytical results are compared with those from ANSYS finite element analysis and experimental modal testing.