• Title/Summary/Keyword: non-animal test

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Effect of Dietary Supplementation with Processed Sulfur on Meat Quality and Oxidative Stability in Longissimus dorsi of Pigs

  • Kim, Ji-Han;Noh, Ha-Young;Kim, Gyeom-Heon;Hong, Go-Eun;Kim, Soo-Ki;Lee, Chi-Ho
    • Food Science of Animal Resources
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    • v.35 no.3
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    • pp.330-338
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    • 2015
  • The effects of dietary supplementation of processed sulfur in pigs according to the level provided during the fattening phase were examined. The pigs were divided into three groups: control (CON), non-sulfur fed pigs; T1, 0.1% processed sulfur fed pigs; T2, 0.3% processed sulfur fed pigs. Physicochemical and sensory properties, as well as meat quality and oxidative stability of the Longissimus dorsi muscle were investigated. The feeding of processed sulfur did not affect moisture and protein contents (p>0.05). However, the crude fat content of T2 was significantly decreased compared to CON (p<0.05), while the pH value of T2 was significantly higher than those of both CON and T1 (p<0.05). Cooking loss and expressible drip of T2 were also significantly lower than that of CON (p<0.05). The redness of meat from T1 was significantly higher than both CON and T2 (p<0.01). During storage, lipid oxidation of the meat from sulfur fed pigs (T1 and T2) was inhibited compared to CON. Examination of omega-3 polyunsaturated fatty acids revealed T2 to have significantly higher content than CON (p<0.05). In the sensory test, the juiciness and overall acceptability of T2 recorded higher scores than CON. This study demonstrated that meat from 0.3% processed sulfur fed pigs had improved nutrition and quality, with extended shelf-life.

Skin Irritation and Sensitization Studies on a Disinfectant Composed of 4-Chloro-m-cresol, Didecyl Dimethyl Ammonium Chloride, and Glutaraldehyde for the Bactericide (4-Chloro-m-cresol, Didecyl Dimethyl Ammonium Chloride 그리고 Glutaraldehyde로 구성된 살균 소독제에 대한 피부 자극성 및 감작성 시험에 관한 연구)

  • Chung, Hee-Sik;Kim, Suk;Chung, Myung-Sun;Jung, Won-Chul;Kim, Dong-Hyeok;Lim, Jeong-Ju;Shon, Ho-Yeong;Kim, Moo-Suk;Kim, Gon-Sup;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • v.22 no.2
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    • pp.110-115
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    • 2007
  • This test was performed to evaluate the skin irritation and sensitization of Creocomplex, disinfectant, Containing 10% 4-chloro-m-cresol, 10% didecyl dimethyl ammonium chloride, and 10% glutaraldehyde. In primary skin irritation test, rabbits were dermally treated with Creocomplex for 24 hrs. The disinfectant did not induce any adverse reactions such as erythema and edema on intact skin sites, but on abraded skin sites, some rabbits showed very slight erythema and edema 24hr after topical application. So, the disinfectant was classified as a practically non-irrifating material based on the score 0.13 of primary irritation index. In the skin sensitization test, guinea pigs were sensitized with intradermal injection of 0.1ml Creocomplex for 24 hr. After 1 week, Creocomplex was treated on the site of injection, and challenged 2 weeks later. Creocomplex did not induce any allergic reactions. Therefore, 10% Creocomplex was graded as a weak material from 0 in both sensitization score· and rate. From results of the present study, it is suggested that 10% Creocomplex does not cause contact irritation and sensitization.

Estimation of Daily Milk Yields from AM/PM Milking Records

  • Lee, Deukhwan;Min, Hongrip
    • Journal of Animal Science and Technology
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    • v.55 no.6
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    • pp.489-500
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    • 2013
  • Daily milk yields on test days were estimated using morning or afternoon partial milk yields collected by official agencies and the accuracy of the estimates was determined. Test-day data for milk yields consisted of 3,156,734 records of AM/PM partial milking measurements of 255,437 milking Holstein cows from 3,708 farms collected from December 2008 to April 2013. A linear regression model (LRM) was applied to estimate daily milk yields using alternate AM/PM milk yield records within lactation stages, milking intervals, and parities on every daily milk yield. The alternate statistical approach was a non-linear hierarchical model (NHM) in which Brody's growth function was implemented by reflecting an animal's physiological milk production cycle. When compared with LRM, daily milk yields predicted by the NHM were assumed to be functionally related to day in milk (or lactation) stage, milking intervals, and partial milk yields. Since the results were in terms of accuracies based on comparisons of different statistical models, accuracies of estimates of daily milk yields by NHM were close to those determined by the LRM. The average of these accuracies was 0.94 for AM partial milk yields and 0.93 for PM partial milk yields for first calving cows. However, the accuracies of AM/PM milk yield estimations from cows under a calving stage higher than the first parity were 0.96 and 0.95, respectively. Correlations between the estimated daily milk yields and the actual daily milk yields ranged from 0.96~0.98. These accuracies were lower for unbalanced AM/PM milking intervals and the first calving cows. Overall, prediction of daily milk yields by NHM would be more appropriate than by LRM due to its flexibility under different milk yield-related circumstances, which provides an idea of the functional relationship between milking intervals and days in milk with daily milk yields from statistical viewpoints.

Quality Assessment of Beef Using Computer Vision Technology

  • Rahman, Md. Faizur;Iqbal, Abdullah;Hashem, Md. Abul;Adedeji, Akinbode A.
    • Food Science of Animal Resources
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    • v.40 no.6
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    • pp.896-907
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    • 2020
  • Imaging technique or computer vision (CV) technology has received huge attention as a rapid and non-destructive technique throughout the world for measuring quality attributes of agricultural products including meat and meat products. This study was conducted to test the ability of CV technology to predict the quality attributes of beef. Images were captured from longissimus dorsi muscle in beef at 24 h post-mortem. Traits evaluated were color value (L*, a*, b*), pH, drip loss, cooking loss, dry matter, moisture, crude protein, fat, ash, thiobarbituric acid reactive substance (TBARS), peroxide value (POV), free fatty acid (FFA), total coliform count (TCC), total viable count (TVC) and total yeast-mould count (TYMC). Images were analyzed using the Matlab software (R2015a). Different reference values were determined by physicochemical, proximate, biochemical and microbiological test. All determination were done in triplicate and the mean value was reported. Data analysis was carried out using the programme Statgraphics Centurion XVI. Calibration and validation model were fitted using the software Unscrambler X version 9.7. A higher correlation found in a* (r=0.65) and moisture (r=0.56) with 'a*' value obtained from image analysis and the highest calibration and prediction accuracy was found in lightness (r2c=0.73, r2p=0.69) in beef. Results of this work show that CV technology may be a useful tool for predicting meat quality traits in the laboratory and meat processing industries.

Immunomodulatory Response Induced by Ginseng

  • Kumar, Ashok
    • Journal of Ginseng Research
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    • v.27 no.3
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    • pp.115-119
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    • 2003
  • There has been continuing interest in the development of synthetic and natural compounds that modify the immune response particularly for the treatment of AIDS and cancer. During the past fifty years, numerous scientific studies have been published on ginseng. Modem human studies have investigated preventive effect of ginseng on several kinds of cancer, its long term immunological effect on HIV patients, its effect on cell mediated immune functions in healthy volunteers. Similarly non clinical studies on animal model system have studied the chemopreventive action of ginseng on cancer and immunological properties of ginseng. The precise mechanism of action of ginseng, however, not clearly understood. Considering its wide-ranging therapeutic effects, this study is being undertaken to elucidate the general mode of action of ginseng, especially to test our hypothesis that its biological action may be mediated by the immune system.

Development of the Endocrine Disruptor Experiment in Teaching Program for Teenagers

  • Yeo, Min-Kyeong;Lim, Eun-Hee
    • Journal of Environmental Science International
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    • v.17 no.8
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    • pp.827-831
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    • 2008
  • We investigated the education process of an environmental experiment in middle and high school. The major objective was that students understand about biological toxicity of endocrine disruptor. We used bisphenol A in environmental experiment to educate them about the effects of endocrine disruptor. The test animal was zebrafish (Danio rerio, wild type) which is very easy to use in the school experiment class. We educated the high school students (n=25) in Suwon through this teaching program. Finally we analyzed the effect of research education between two groups, education students (test) and non-education students (control). This experiment showed there were some positive effects on environmental reorganization of students. They understood the affect of endocrine disruptors in fish and man. Specifically, understanding the importance of preservation of ecosystem increased in the test-education group.

Studies on the Survival Rate of Bisected Porcine Embryos and Immature Oocytes (돼지 분할 수정란 및 미성숙란의 생존율에 관한 연구)

  • 김상근;이명헌;서길웅
    • Korean Journal of Animal Reproduction
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    • v.19 no.2
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    • pp.129-134
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    • 1995
  • This study was carried out to investigate on the survival rate and in vitro developmental rate of bisected porcine embryos and immature oocytes by manipulator and micropipette. Bisected embryos and immature oocytes cultured for 1∼5 days in TCM 199 medium with 20% FCS. Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The survival rate of bisected porcine embryos and oocytes were 26.1%, 22.7%, respectively. The survival rate of bisected embryos and oocytes was significantly lower than that of non-bisection embryos(62.5%). 2. The survival rate of bisected porcine embryos in cultured for 12, 24, 48, 72 hrs with 20% FCS+TCM-199 medium were 26.9%, 19.2%, 19.2% and 11.5%, respectively. 3. The in-vitro developmental rate with and without zona pellucida of bisected porcine embryos by micromanipulator were 30.8%, 25.0%, respectively.

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Studies on Effects of Cryoprotectants in the Medium on the Survival Rate of Rapidly Frozen Porcine Bisected Demi-Embryos (돼지 분할 수정란의 급속동결 융해후 생존율에 관한 연구)

  • 오원진;김상근
    • Korean Journal of Animal Reproduction
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    • v.18 no.1
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    • pp.31-37
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    • 1994
  • This study carried out to investigate the effects of cryoprotectants in the medium on the survival rate of rapidly frozen porcine bisected demi-embryos. The porcine bisected demi-embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 3$0^{\circ}C$ water bath. Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows : 1. The survival rates of without-zona pellucida embryos and 2 blastomeres porcine embryos were 10.0 and 7.1%, respectively. The rate of unfrozen blastomeres (20.00%) was significantly higher than that of non-frozen oocytes. 2. The survival rates of with and without-zona pellucida of bisected porcine embryos by micromanipulator were 20.0 and 14.3%, respectively. 3. The developmental rates of with and without-zona pellucida of bisected poricine embryos by micromanipulator were 13.3 and 7.1%, respectively.

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Effects of Feeding Non-Salt Diet on the Induced Molting in Laying Hens (무염 사료의 급여가 유도환우에 미치는 영향)

  • Hong, E.C.;Na, J.C.;You, D.C.;Kim, H.K.;Chung, W.T.;Lee, H.J.;Kim, I.H.;HwangBo, J.
    • Korean Journal of Poultry Science
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    • v.34 no.4
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    • pp.279-286
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    • 2007
  • This study was conducted to induce molting with DDGS and non-salt diet and compare the effect of feeding molting and fasting molting on the performance, egg quality, and visceral organs in laying hens for animal welfare. One-hundredeight 62-wk-old White Leghorn hens that egg production was over 80% and average weight was $1.8{\pm}0.1kg$ were used in this study. Treatments were control(non-molt treatment), feeding molt treatment(DDGS, non-salt diet), and fasting molt treatment. The four treatments were administered to three replicate group of nine hens wherein each group. All treatment groups were fed the basal diet(CP 15%, ME 2,700 kal/kg) for two weeks as the adaptation period. Test Periods were 28 days at all treatments. Egg production decreased for 18 days to be 0% at feeding molting treatment, and for 17 days to be 0% at non-salt feeding molting treatment. Egg production stopped for 6 days at fasting molting treatment. Egg production restarted after 12 days molt at feeding molting treatment, while after 16 days at fasting molting treatment. On the egg quality was improved at molting treatments (p<0.05) except egg yolk. Egg shell tissue was crowded at molting treatment to compare to control. Liver weights, heart weight, and oviduct weight of laying hens decreased at molting treatments(p<0.05). Finally, feeding molting might could be replaced fasting molting on the welfare and further studies were needed about molting program.

The Change in Quality Characteristics of Hanwoo in Home Meal Replacement Products under Different Cooking and Freezing Methods

  • Kim, Honggyun;Park, Dong Hyeon;Hong, Geun-Pyo;Lee, Sang-Yoon;Choi, Mi-Jung;Cho, Youngjae
    • Food Science of Animal Resources
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    • v.38 no.1
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    • pp.180-188
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    • 2018
  • The market size of home meal replacement (HMR) products has been gradually growing worldwide, even in Korea. In Korean HMR products, meat is the most important food ingredient compared with rice and vegetables. Therefore, this study aimed to evaluate changes in physiochemical and sensory aspects of beef under different preparation processes. For preparing four treatments, beef eye of round (ER) added with salt and sugar (treatment 1) and that without salt and sugar (treatment 2) were mixed with rice and frozen at $-50^{\circ}C$. Beef ER without salt and sugar was also topped onto the rice and frozen (treatment 3), and that was topped onto the rice and precooled before freezing (treatment 4). Physiochemical analyses included cooking and drip losses, shear force, color, salt soluble protein, and sensory attributes were tested. The results showed significantly higher drip loss and total loss in beef ER samples 1 and 2, which were mixed with rice, compared to beef ER samples 3 and 4, which were not mixed with rice. A significantly higher discoloration was also observed in beef ER samples 1 and 2, compared to that in samples 3 and 4. In the partial least squares regression (PLSR) analysis, beef ER sample 4 (precooled before freezing) was highly related to sensory attributes, such as flavor, overall acceptability, and juiciness, and far from non-preferred shear force. As a result, beef ER in HMR sample 4 was the most preferable to the sensory panel, and it had the most desirable physicochemical analysis outcomes.