• Title/Summary/Keyword: new strain

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Improved Genetic Algorithm-Based Damage Detection Technique Using Natural Frequency and Modal Strain Energy (고유진동수와 모드변형에너지를 이용한 향상된 유전알고리즘 기반 손상검색기법)

  • Park Jae-Hyung;Ryu Yeon-Sun;Yi Jin-Hak;Kim Jeong-Tae
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.19 no.3 s.73
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    • pp.313-322
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    • 2006
  • In the genetic algoricm (GA) based damage detection methods using vibration of structures, the selection of modal properties is important to improve the accuracy of damage detection. The objective of this study is to improve the accuracy of damage detection using natural frequency and modal strain energy, The following approaches are used to achieve the goal. First, modal strain energy is formulated and a new GA-based damage detection technique using natural frequency and modal strain energy is proposed. Next, to verify the efficiency of proposed technique, damage scenarios for free-free beam are designed and vibration modal tests of the target structure are conducted. Finally, the feasibility of the proposed technique is verified in comparison with other GA-based damage detection technique using natural frequency and mode shape.

Gibberellins Production and Identification of Endophytic Fungi Isolated from Aquatic Plant in Fresh Water (담수에 자생하는 수생식물에서 분리된 내생균류의 지베렐린 생산과 동정)

  • You, Young-Hyun;Kang, Sang-Mo;Choi, Yu-Mi;Lee, Myung-Chul;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.43 no.1
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    • pp.71-76
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    • 2015
  • Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) ($GA_1$, 1.2 ng/100 mL; $GA_4$, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.

Isolation of an acid-tolerant Leuconostoc mesenteroides LM3 from Kimchi (김치에서 산내성을 가진 Leuconostoc mesenteroides LM3의 분리)

  • 사금희;백상규;윤혜선;강경희;정진국;김일섭;문혜연;김사열;유춘발
    • Journal of Life Science
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    • v.12 no.6
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    • pp.812-820
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    • 2002
  • In order to understand stress response of Leuconostoc mesenteroides against lactic acid, a new Leuconostoc sp. which has acid tolerance was isolated from various Kimchi samples. And it identified as Leuconostoc mesenteroides LM3 by comparing its fatty acid composition with reference strain. Its growth pattern was investigated by adding a given concentration of lactic acid at the lag phase to the stationary phase. In the DeMan, Rogosa, Sharpe (MRS) media containing over 0.4% (final v/v) lactic acid, this strain slowed slowly After exposure of the stationary phase cells to 4% of lactic acid for 60 min, this strain could survive, whereas a reference strain, Leuconostoc mesenteroides KCTC3505, showed no survival. And changes of trehalose concentration, the activity of trehalase and ATPase in the growth of Leuconostoc mesenteroides LM3 after addition of 0.6% (final v/v) lactic acid were investigated : After exposure to lartic acid, trehalose concentration in this strain was increased in comparison with no treatment, but its trehalase activity was not changed. And its ATPase activity was constant, and intracellular pH was almost constant. This result meant Leuconostoc mesenteroides LM3 should have a tolerance against lactic acid. It remains to further study the mechanism of this acid tolerance.

Plasticity and Fracture Behaviors of Marine Structural Steel, Part III: Experimental Study on Failure Strain (조선 해양 구조물용 강재의 소성 및 파단 특성 III: 파단 변형률에 관한 실험적 연구)

  • Choung, Joon-Mo;Shim, Chun-Sik;Kim, Kyung-Su
    • Journal of Ocean Engineering and Technology
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    • v.25 no.3
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    • pp.53-65
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    • 2011
  • This is the third of several companion papers dealing with the derivation of material constants for ductile failure criteria under hydrostatic stress. It was observed that the ultimate engineering stresses and elongations at fracture from tensile tests for round specimens with various notch radii tended to increase and decrease, respectively, because of the stress triaxiality. The engineering stress curves from tests are compared with numerical simulation results, and it is proved that the curves from the two approaches very closely coincide. Failure strains are obtained from the equivalent plastic strain histories from numerical simulations at the time when the experimental engineering stress drops suddenly. After introducing the new concept of average stress triaxiality and accumulated average strain energy, the material constants of the Johnson-Cook failure criterion for critical energies of 100%, 50%, and 15% are presented. The experimental results obtained for EH-36 steel were in relatively good agreement with the 100% critical energy, whereas the literature states that aluminum fits with a 15% critical energy. Therefore, it is expected that a unified failure criterion for critical energy, which is available for most kinds of ductile materials, can be provided according to the used materials.

Genetic variation of local varieties and mutants groups induced by gamma ray in Hypsizigus marmoreus (느티만가닥버섯의 재래종과 감마선 돌연변이체들의 유전적 변이)

  • Kim, Jong-Bong;Yu, Dong-Won
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.181-186
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    • 2014
  • This research was carried out to analyze the genetic variation of 18 wild strain, 2 breed varieties and 20 mutants of Hypsizygus marmoreus by random amplification of polymorphic DNA(RAPD). Also, 3 strains of Lyophyllum decartes and 1 strain of Lyophyllum shimeji were used. These mushrooms were collected from korea, china, Taiwan and Japan. Spores of H. marmoreus JV-2 strain were irradiated by gamma ray for mutagenesis. 40 kind of primers were used for this reaserch. Number of reaction primer were 31. Electrophorectic patterns of RAPD showed genetic variation. In phylogenetic tree, they were divided into seven group. Discriminative differences were observed between wild strain and mutants in H. marmoreus. These results might suggest that these primers and gamma ray irradiation of spores were useful tools for developing new strain for mushroom.

Halobacillus blutaparonensis sp. nov., a Moderately Halophilic Bacterium Isolated from Blutaparon portulacoides Roots in Brazil

  • Barbosa Deyvison Clacino;Bae Jin-Woo;Weid Irene Von Der;Vaisman Natalie;Nam Young-Do;Chang Ho-Won;Park Yong-Ha;Seldin Lucy
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1862-1867
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    • 2006
  • A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated $M9^T$, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain $M9^T$ had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-$C_{15:0}$ and iso-$C_{15:0}$ were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $M9^T$ belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain $M9^T$ with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21% and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain $M9^T$ (=ATCC BAA-$1217^T$, =CIP $108771^T$, =KCTC $3980^T$) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.

The Impact of Job Strain, Life Satisfaction, and the Division of Household Labor on Fertility Rates across OECD Countries (직무긴장, 삶의 만족도, 그리고 가사노동 분배가 OECD 국가들의 출산율에 미치는 영향)

  • Jeon, Seung Bong
    • The Journal of the Korea Contents Association
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    • v.20 no.8
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    • pp.251-261
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    • 2020
  • This study aims to suggest a new perspective that can account for variations in fertility rates across OECD countries. Most previous literature has highlighted the influence of government policies on fertility rates. This study focuses the role of job strain, unequal division of household labor, and life satisfaction on fertility rates. These factors are related to work-life balance, and play a crucial role in understanding variations in fertility rates across OECD countries. Using fuzzy set qualitative comparative research analysis (fsQCA), this study tests whether fertility rates can be explained by differences in the levels of job strain, gender equality at home, and life satisfaction across countries. The results are as follows: First, high fertility-countries show low levels of job strain, equal division of household labor, high levels of life satisfaction, and high levels of GDP. Second, a high level of GDP is not crucial for achieving high fertility rates. This study suggests that changes in working conditions and organizational culture are required to increase the fertility rate in Korea, since this can influence work-life balance, life satisfaction and equal division of household labor.

Bending ratcheting behavior of pressurized straight Z2CND18.12N stainless steel pipe

  • Wang, Lei;Chen, Gang;Zhu, Jianbei;Sun, Xiuhu;Mei, Yunhui;Ling, Xiang;Chen, Xu
    • Structural Engineering and Mechanics
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    • v.52 no.6
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    • pp.1135-1156
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    • 2014
  • The ratcheting effect greatly challenges the design of piping components. With the assistance of the quasi-three point bending apparatus, ratcheting and the ratcheting boundary of pressurized straight Z2CND18.12N stainless steel pipe under bending loading and vertical displacement control were studied experimentally. The characteristics of progressive inelastic deformation in axial and hoop directions of the Z2CND18.12N stainless steel pipes were investigated. The experiment results show that the ratcheting strain occurs mainly in the hoop direction while there is less ratcheting strain in the axial direction. The characteristics of the bending ratcheting behavior of the pressure pipes were derived and compared under load control and displacement control, respectively. The results show that the cyclic bending loading and the internal pressure affect the ratcheting behavior of the pressurized straight pipe significantly under load control. In the meantime, the ratcheting characteristics are also highly associated with the cyclic displacement and the internal pressure under displacement control. All these factors affect not only the saturation of the ratcheting strain but the ratcheting strain rate. A series of multi-step bending ratcheting experiments were conducted under both control modes. It was found that the hardening effect of Z2CND18.12N stainless steel pipe under previous cyclic loadings no matter with high or low displacement amplitudes is significant, and the prior loading histories greatly retard the ratcheting strain and its rate under subsequent loadings. Finally, the ratcheting boundaries of the pressurized straight Z2CND18.12N stainless steel pipe were determined and compared based on KTA/ASME, RCC-MR and the experimental results.

Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil

  • Long, Dongyan;Tang, Xianjin;Cai, Kuan;Chen, Guangcun;Shen, Chaofeng;Shi, Jiyan;Chen, Linggui;Chen, Yingxu
    • Journal of Microbiology and Biotechnology
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    • v.23 no.8
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    • pp.1123-1132
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    • 2013
  • The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.

Dynamic Analysis of Plates using a Improved Assumed Natural Strain Shell Element (개선된 자연변형률 쉘 요소를 이용한 판의 진동해석)

  • Lee, Won-Hong;Han, Sung-Cheon;Park, Weon-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.6
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    • pp.2284-2291
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    • 2010
  • In this paper, we investigate the vibration analysis of plates, using an 8-node shell element that accounts for the transverse shear strains and rotary inertia. The forced vibration analysis of plates subjected to arbitrary loading is investigated. In order to overcome membrane and shear locking phenomena, the assumed natural strain method is used. To improve an 8-node shell element for forced vibration analysis, the new combination of sampling points for assumed natural strain method was applied. The refined first-order shear deformation theory based on Reissner-Mindlin theory which allows the shear deformation without shear correction factor and rotary inertia effect to be considered is adopted for development of 8-node assumed strain shell element. In order to validate the finite element numerical solutions, the reference solutions of plates are presented. Results of the present theory show good agreement with the reference solution. In addition the effect of damping is investigated on the forced vibration analysis of plates.