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Isolation of an acid-tolerant Leuconostoc mesenteroides LM3 from Kimchi

김치에서 산내성을 가진 Leuconostoc mesenteroides LM3의 분리

  • 사금희 (경북대학교 대학원 미생물학과) ;
  • 백상규 (경북대학교 대학원 미생물학과) ;
  • 윤혜선 (경북대학교 대학원 미생물학과) ;
  • 강경희 (경북대학교 대학원 미생물학과) ;
  • 정진국 (경북대학교 대학원 미생물학과) ;
  • 김일섭 (경북대학교 대학원 미생물학과) ;
  • 문혜연 (경북대학교 대학원 미생물학과) ;
  • 김사열 (경북대학교 대학원 미생물학과) ;
  • 유춘발 (대구대학교 식품공학과)
  • Published : 2002.12.01

Abstract

In order to understand stress response of Leuconostoc mesenteroides against lactic acid, a new Leuconostoc sp. which has acid tolerance was isolated from various Kimchi samples. And it identified as Leuconostoc mesenteroides LM3 by comparing its fatty acid composition with reference strain. Its growth pattern was investigated by adding a given concentration of lactic acid at the lag phase to the stationary phase. In the DeMan, Rogosa, Sharpe (MRS) media containing over 0.4% (final v/v) lactic acid, this strain slowed slowly After exposure of the stationary phase cells to 4% of lactic acid for 60 min, this strain could survive, whereas a reference strain, Leuconostoc mesenteroides KCTC3505, showed no survival. And changes of trehalose concentration, the activity of trehalase and ATPase in the growth of Leuconostoc mesenteroides LM3 after addition of 0.6% (final v/v) lactic acid were investigated : After exposure to lartic acid, trehalose concentration in this strain was increased in comparison with no treatment, but its trehalase activity was not changed. And its ATPase activity was constant, and intracellular pH was almost constant. This result meant Leuconostoc mesenteroides LM3 should have a tolerance against lactic acid. It remains to further study the mechanism of this acid tolerance.

이 연구는 주변의 김치들을 수거하여 젖산에 내성을 가진 Leuconostoc mesenteroides을 선택배지를 이용하여 분리하고, 지방산 조성을 분석하여 동정한 결과, Leuconostoc mesenteroides LM3균주로 동정했다. 이어서 그의 성장패턴을 관찰하고, 유도기와 지수성장기에서 젖산을 첨가한 결과 성장저해가 일어났음을 확인했으며, 젖산의 최종농도가 0.4%이상인 배지에서는 성장속도가 느려짐을 알았다. 그리고 정지기시작단계에서 고농도의 젖산을 단시간 처리하였을 때, 대조균주가 생존하지 못하는 4%농도에서도 Leuconostoc mesenteroides LM3군주가 생존하는 것을 확인하여 산에 대한 내성이 뛰어남을 알 수 있었다. 이에 trehalose 농도의 변화, 그리고 trehalase와 ATPase 활성을 측정하였고, 이를 통해 젖산에 의한 세포내의 변화를 관찰하였다. 그 결과, 각 성장시기별로 0.6% 젖산을 첨가했을 때 Leuconostoc mesenteroides LM3균주가 trehalose 축적률이 높아졌으나 trehalase활성엔 큰 변화가 없었다. ATPase 활성은 감소하는 경향을 보였으나 시기별 활성은 일정하였고, 세포내 pH를 측정한 결과 Leuconostoc mesenteroides LM3균주는 pH 6으로 일정하게 유지되었다.

Keywords

References

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