• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.312-316
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• 2004

Five strains, producing bacterial thermostable L-arabinose isomerase, were isolated from Korean soil samples obtained from compost under high temperature circumstances. Among these strains, the CBG-Al showed the highest L-arabinose isomerase activity at $60^\circ{C}$ and was selected as a D-tagatose producing strain from D-galactose. This strain was identified as Geobacillus thermodenitrificans based on the 16S rRNA analysis, and biological and biochemical characteristics. The isolated strain was aerobic, rod-shaped, Gram-positive, nonmotile, and an endospore-forming bacterium. No growth was detected in culture temperature below $40^\circ{C}$. The maximum growth temperature and maximum temperature of enzyme activity were $75^\circ{C}$ and $65^\circ{C}$, respectively. In metal ion effects, $Ca^{2+}$ was the most effective enzyme activator with the reaction rate by 150%. In a 5-1 jar fermentor with 3-1 MY medium, L-arabinose isomerase activity was growth-associated and pH decreased rapidly after the initial logarithmic phase.

• Transactions of Materials Processing
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• v.22 no.8
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• pp.456-462
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• 2013

Compression tests were conducted at the various temperatures and strain rates to investigate void formation and microstructures behavior of a 1.9wt%C ultrahigh carbon steel used in forged workrolls. The microstructure, grain size and volume fraction of cementite were determined using specimens deformed in the temperature range from 800 to $1150^{\circ}C$ and strain rates from 0.01 to 10/s. It was found from the microstructural analysis that the grain size is larger at higher temperatures and lower strain rate deformation conditions. In addition, a higher volume fraction of cementite was measured at lower temperatures. The brittle blocky cementite was fractured at $800^{\circ}C$ and $900^{\circ}C$ regardless of strain rate. As a result, numerous new micro voids were formed in the fragmented blocky cementite. It was also found that local melting can occur at temperatures of more than $1130^{\circ}C$. Therefore, the forging temperature should be controlled between $900^{\circ}C$ and $1120^{\circ}C$. The temperature rise, which depends on the anvil stroke and velocity, was estimated through cogging simulation to find the appropriate forging temperature and to prevent local melting due to plastic work.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.1015-1018
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• 2006

In this paper, new topology optimization technique is proposed. It mainly uses the strain energy distributions induced by the mode shapes associated with natural frequencies of the structure and so we can implicitly consider the dynamic characteristics of the structure in the topology optimization process. The strain energy to be minimized is employed as the objective function and the initial volume of structures is adopted as the constraint function. The resizing algorithm devised from the optimality criteria method is used to update the hole size of the cell existing in each finite element. The cantilever beam problem is adopted to test the proposed techniques. From numerical test, it is found to be that the optimum topology of the cantilever produced by the proposed technique has a hugh increase of natural frequency value and the technique is very effective to maximize the fundamental frequency of the structure.

• Smart Structures and Systems
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• v.1 no.4
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• pp.385-404
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• 2005

The paper presents a model to calculate reinforcement strain using measured crack width in members under applied tension, flexure, and/or shear stress. Crack mapping using a new type of distributed coaxial cable sensors for health monitoring of large-scale civil engineering infrastructure was recently proposed and developed by the authors. This paper shows the results and performance of such sensors mounted on near surface of two flexural beams and a large scale reinforced concrete box girder that was subjected to cyclic combined shear and torsion. The main objectives of this health monitoring study was to correlate the sensor's response to strain in the member, and show that magnitude of the signal's reflection coefficient is related to increases in applied load, repeated cycles, cracking, and reinforcement yielding. The effect of multiple adjacent cracks, and signal loss was also investigated. The results shown in this paper are an important step in using the sensors for crack mapping and determining reinforcement strain for in-situ structures.

• International Journal of Naval Architecture and Ocean Engineering
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• v.11 no.2
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• pp.671-678
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• 2019

In this paper, the low cycle fatigue failure and ratcheting behavior, as well as their interaction of AH32 steel were experimentally investigated under uniaxial cyclic loading. The effects of mean stress, stress amplitude and stress ratio on the low cycle fatigue life and ratcheting strain were discussed. It was found that the ratcheting strain increased while the fatigue life decreased with the increase of mean stress and stress amplitude, and the increasing stress ratio would result in smaller ratcheting and larger fatigue life. Two kinds of failure modes, i.e. low cycle fatigue failure due to crack propagates and ratcheting failure due to large plastic strain will take place respectively. Based on the experimental results, considered the effect of ratcheting on fatigue life, a model with the maximum stress and ratcheting strain rate was proposed. Comparison with the experimental result showed that the new model provided a good prediction for AH32 steel.

• Korean Journal of Optics and Photonics
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• v.22 no.1
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• pp.1-4
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• 2011

A new fabrication technique of long-period fiber gratings with periodically surface-etched structures is proposed. Transmission characteristics of the periodically surface-etched long-period fiber gratings are improved by changing strain because of variation of coupling strength between the core and the cladding modes. The sensitivities of the periodically surface-etched long-period fiber gratings to strain, torsion, and ambient refractive index were measured. The strain and the torsion sensitivities were measured to be $-0.033\;dB/\mu\varepsilon$ and -1.30 nm m/rad, respectively. The ambient index sensitivity was measured to be -31.33 nm/RIU from a range from 1.33 to 1.42.

• Journal of Microbiology
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• v.43 no.6
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• pp.499-502
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• 2005

Methylophaga sp. strain SK1 is a new restricted facultative methanol-oxidizing bacterium that was isolated from seawater. The aim of this study was to characterize the electron carriers involved in the methanol oxidation process in Methylophaga sp. strain SK1. The gene encoding cytochrome $c_L$ (mxaG) was cloned and the recombinant gene was expressed in Escherichia coli $DH5\alpha$ under strict anaerobic conditions. The recombinant cytochrome $c_L$ had the same molecular weight and absorption spectra as the wild-type cytochrome $c_L$ both in the reduced and oxidized forms. The electron flow rate from methanol dehydrogenase (MDH) to the recombinant cytochrome $c_L$ was similar to that from MDH to the wild-type cytochrome $c_L$. These results suggest that recombinant cytochrome $c_L$ acts as a physiological primary electron acceptor for MDH.

• KSBB Journal
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• v.16 no.4
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• pp.370-375
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• 2001

For the production of new exo-biopolymers from microorganisms, an exe-biopolymer producing bacterial strain was isolated from the composter used in composting of organic wastes. Bacteriological properties of this strain and physicochemical properties of producing exo-biopolymer were investigated. The isolated strain was identified as Enterobacter sp. through its morphological, cultural and physiological characteristics. The results of color reactions, CPC (cetyl pyridinium chloride) precipitation and infra red absorption spectral analysis indicated that this exo-biopolymer was presumed as an acidic polysaccharide with uronic acid. This polysaccharide was identified as hetero-polysaccharide consisting of galactose, mannose and galacturonic acid by gas chromatography, and the molecular weight of exopolysaccharide purified by gel chromatography were about 370,000 daltons. The polysaccharide solutions(0.50-2.0%, w/v) exhibited non-Newtonian flow behavior with pseudoplastic property and showed the ability of gel formation at above 1.5% (w/v) of polysaccharide concentration.

• Structural Engineering and Mechanics
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• v.24 no.2
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• pp.137-154
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• 2006

The reliable pushover analysis of RC structures requires a realistic prediction of moment-curvature relations, which can be obtained by utilizing proper constitutive models for the stress-strain relationships of laterally confined concrete members. Theoretical approach of Mander is still a single stress-strain model, which employs a multiaxial failure surface for the determination of the ultimate strength of confined concrete. Alternatively, this paper introduces a simple and practical failure criterion for confined concrete with emphasis on introduction of significant modifications into the two-parameter Drucker-Prager model. The new criterion is only applicable to triaxial compression stress state which is exactly the case in the RC columns. Unlike many existing multi-parameter criteria proposed for the concrete fracture, the model needs only the compressive strength of concrete as an independent parameter and also implies for the influence of the Lode angle on the material strength. Adopting Saenz equation for stress-strain plots, satisfactory agreement between the measured and predicted results for the available experimental test data of confined normal and high strength concrete specimens is obtained. Moreover, it is found that further work involving the confinement pressure is still encouraging since the confinement model of Mander overestimates the ultimate strength of some RC columns.

• Steel and Composite Structures
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• v.29 no.4
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• pp.465-481
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• 2018

The use of external carbon-fiber-reinforced polymer (CFRP) laminates is one of the most effective techniques existing for the confinement of circular concrete specimens. Currently, several researches have been made to develop models for predicting the ultimate conditions of this type of confinement. As most of the major existing models were developed based on limited experimental database. This paper presents the development of new confinement ultimate conditions, strength and strain models, for concrete cylinders confined with CFRP composites based on a statistical analysis of a large existing experimental database of 310 cylindrical concrete specimens wrapped with CFRP. The database is used to evaluate the performance of the proposed and major existing strength and strain models. Based on the two different statistical indices, the coefficient of determination ($R^2$) and the Root Mean Square Error (RMSE), the two proposed confinement ultimate conditions presents a good performance compared to the major existing models except the models of Lam and Teng (2003) and Youssef et al. (2007) which have relatively similar performance to the proposed models.