Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characterization of Methylophaga sp. strain SK1 Cytochrome $c_L$ Expressed in Escherichia coli

  • Kim, Hee-Gon (Department of Biomaterials Engineering, Chosun University) ;
  • Phan, Trongnhat (Department of Biomaterials Engineering, Chosun University) ;
  • Jang, Tae-Sa (Department of Chemistry, Chosun University) ;
  • Koh, Moon-Joo (Department of Environmental Engineering, Chosun University) ;
  • Kim, Si-Wouk (Department of Environmental Engineering, Gwangju University)
  • Published : 2005.12.01
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Abstract

Methylophaga sp. strain SK1 is a new restricted facultative methanol-oxidizing bacterium that was isolated from seawater. The aim of this study was to characterize the electron carriers involved in the methanol oxidation process in Methylophaga sp. strain SK1. The gene encoding cytochrome $c_L$ (mxaG) was cloned and the recombinant gene was expressed in Escherichia coli $DH5\alpha$ under strict anaerobic conditions. The recombinant cytochrome $c_L$ had the same molecular weight and absorption spectra as the wild-type cytochrome $c_L$ both in the reduced and oxidized forms. The electron flow rate from methanol dehydrogenase (MDH) to the recombinant cytochrome $c_L$ was similar to that from MDH to the wild-type cytochrome $c_L$. These results suggest that recombinant cytochrome $c_L$ acts as a physiological primary electron acceptor for MDH.

Keywords

References

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