• Title/Summary/Keyword: new purity

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Isolation and Purification of Lectin from Phaseolus radiatus (녹두로부터 렉틴 성분의 분리 정제)

  • 정시련;홍승수;전경희
    • YAKHAK HOEJI
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    • v.27 no.3
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    • pp.221-227
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    • 1983
  • New lectins, lymphoagglutinating lectins from mung beans (MBLA) have been isolated and purified. Mung beans crude extracts were made with 0.15M NaCl and these were purified through anionic exchange chromatography. Four fractions were obtained from DEAE Sephadex A-50 by salt gradients elution. Lectin activity, enzyme activity, protein assay, identification of purity by polyacrylamide gel electrphoresis and immunochemical studies were carried out with these four fractions. Through these results, it can be suggested that 0.2M fraction is newly found potent MBLA. There were some relationships with MBLA and L-PHA but no similarities were observed between MBLA and E-PHA.

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Synthesis of Sulfide-based Phosphors by Multiply Combined Methods Based Upon Inorganic Chemistry

  • Kakihana, Masato;Petrykin, Valery
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.121-124
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    • 2009
  • We developed a panel of innovative solution-based approaches for synthesis of multicomponent sulfide phosphor materials. The application of these synthesis strategies allowed for preparation of high-purity and high-brightness $BaAl_2S_4:Eu$ and $Ba_2SiS_4:Eu$ phosphors and led to the discovery of new phosphors suitable for application in the LED-based lighting including $Sr_2ZnS_3:Eu$, $CaBaSiS_4:Eu$ and $(Ba,Sr)_2ZnS_3:Eu$.

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Radiosynthesis of 125I-labeled 2-cyanobenzothiazole: A new prosthetic group for efficient radioiodination reaction

  • Mushtaq, Sajid;Choi, Dae Seong;Jeon, Jongho
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.3 no.1
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    • pp.44-51
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    • 2017
  • Herein we report an efficient radiolabeling method based on a rapid condensation reaction between N-terminal cysteine and 2-cyanobenzothiazole (CBT). Radioiodination of 2-cyano-6-hydroxybenzothiazole 2 was carried out using chloramine-T to give $^{125}I$-labeled CBT ([$^{125}I$]1) with a high radiochemical yield ($90{\pm}6%$ isolated yield, n=3) and radiochemical purity (>99%). To evaluate the radiolabeling efficiency of $^{125}I$-labeled CBT, model compounds, L-cysteine and N-terminal cysteine conjugated cRGD peptide were reacted with [$^{125}I$]1 under mild conditions. The radiolabeling reactions rapidly provided the $^{125}I$-labeled products [$^{125}I$]5 and [$^{125}I$]6 with excellent radiochemical yields and radiochemical purity. Therefore, we demonstrate that [$^{125}I$]1 will be a useful prosthetic group for radioactive iodine labeling of N-terminal cysteine bearing biomolecules.

Fluorescent Blue Materials for Efficient Organic Light-Emitting Diode with High Color Purity

  • Choi, Kyung-Sun;Lee, Chan-Hyo;Lee, Kwan-Hee;Park, Su-Jin;Son, Seung-Uk;Chung, Young-Keun;Hong, Jong-In
    • Bulletin of the Korean Chemical Society
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    • v.27 no.10
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    • pp.1549-1552
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    • 2006
  • We report a new series of blue dopants composed of both electron donating and electron accepting moieties in one molecule, based on nalidixic acid. The EL device derived from the dopant exhibits pure blue light emission (0.15, 0.14) The current efficiency is estimated to be 3.88 cd/A at 100 $cd/m^2$, which shows remarkable enhancement, compared to that of the host itself (2.5 cd/A at 100 $cd/m^2$) under the same conditions. These results demonstrate that the incorporation of a proper guest into the host in a guest-host doped system improves not only the purity of the fluorescent blue emission but also elevates its quantum efficiency, thus improving the OLED performance.

A New large-scale Pre-purification for Peroxidase from Plant Cell Cultures (식물세포 배양으로부터 Peroxidase 대량 정제를 위한 전처리 공정 개발)

  • 표상현
    • KSBB Journal
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    • v.15 no.4
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    • pp.342-345
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    • 2000
  • A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced.

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Synthesis of High purity $TiO_2$ Powder from Elemental Titanium Particles (금속티타늄 분말로부터 고순도 $TiO_2$ 미분말 합성)

  • 조태환;송기세;이용민;송종택
    • Journal of the Korean Ceramic Society
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    • v.30 no.3
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    • pp.222-228
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    • 1993
  • In order to obtian TiO2 fine powder of high purity, the new method which is different from the sulfate process and the chloride one was employed. TiO2 was syntehsized by the reaction between elemental titanium particles and H2O2 solutiosn at 30~7$0^{\circ}C$, and then TiO2 powder was characterized using XRD, SEM, TEM, DTA and FT-IR. It was found that the initial reaction rate was fast at a high temperature due to the high generation of activated oxygen associated with thedecomposition of H2O2. However, the reaction was slowly proceeded at a low temperature due to slow decomposition of H2O2. In this experimental range, the optimum temeprature was ocnsidered to be about 5$0^{\circ}C$. The primary particles of the hydrous titanium dioxide (TiO2 gel) before aging were spherical, and their mean sizes were about 50nm. The similar shapes and sizes were observed with calcinatin at 40$0^{\circ}C$. The FT-IR spectrum of Ti-OH in the TiO2 gel powder appeared at around 3380cm-1, 1630cm-1 and 530cm-1. This gel powder was crystallized into the anatase type TiO2 at 300~40$0^{\circ}C$.

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Study on the development of small-scale hydrogen production unit using steam reforming of natural gas (천연가스 개질 방식 중소형 고순도 수소제조 장치 개발 연구)

  • Seo, Dong-Joo;Chue, Kuck-Tack;Jung, Un-Ho;Park, Sang-Ho;Yoon, Wang-Lai
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.06a
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    • pp.720-722
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    • 2009
  • This work is mainly focused at developing the hydrogen production unit with the capacity of 20 $Nm^3/h$ of high purity hydrogen. At present steam reforming of natural gas is the preferable method to produce hydrogen at the point of production cost. The developed hydrogen production unit composed of natural gas reformer and pressure swing adsorption system. To improve the thermal efficiency of steam reforming reactor, the internal heat recuperating structure was adopted. The heat contained in reformed gas which comes out of the catalytic beds recovered by reaction feed stream. These features of design reduce the fuel consumption into burner and the heat duty of external heat exchangers, such as feed pre-heater and steam generator. The production rate of natural gas reformer was 41.7 $Nm^3/h$ as a dryreformate basis. The composition of PSA feed gas was $H_2$ 78.26%, $CO_2$ 18.49%, CO 1.43% and $CH_4$ 1.85%. The integrated production unit can produce 21.1 $Nm^3/h$ of high-purity hydrogen (99.997%). The hydrogen production efficiency of the developed unit was more than 58% as an LHV basis.

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Isolation and Characterization of Chondroitin Sulfates from the Byproducts of Marine Organisms

  • Im, A-Rang;Sim, Joon-Soo;Park, You-Mie;Hahn, Bum-Soo;Toida, Toshihiko;Kim, Yeong-Shik
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.872-877
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    • 2009
  • By-products of marine organisms including salmon, skate, flatfish, and yellow goosefish were investigated to search for new source of chondroitin sulfate (CS). Agarose gel electrophoresis with chondroitinase depolymerization showed that purified chondroitin sulfate did not contain any other glycosaminoglycans. 1H-nuclear magnetic resonance (NMR) spectra were acquired to confirm the structure and purity. The average molecular weight ranging from 22 to 64 kDa was determined by high performance size exclusion chromatography. Disaccharide compositions and purities were determined by strong anion exchange-high performance liquid chromatography (SAX-HPLC) after chondroitinase ABC depolymerization. SAX-HPLC data exhibited that the purity was from $81.7{\pm}1.3$ to $114.2{\pm}2.5%$ and the yield was from 1.3 to 12.5%. All analytical results indicate that salmon cartilage, skate cartilage, and yellow goosefish bone could be promising sources of CS to substitute shark cartilage CS in commercial neutraceuticals.

Aesthetic Characteristics of the Ballerina Look Presented in Modern Fashion - Focused on the Design Since 1990 - (현대패션에 표현된 발레리나 룩의 미적 특성 -1990년 이후 디자인을 중심으로-)

  • 김선영
    • Journal of the Korean Society of Costume
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    • v.54 no.6
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    • pp.41-52
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    • 2004
  • The objective of this study is to contemplate both the formation and the aesthetic characteristics of the ballerina look represented in modern fashion by analyzing a ballet costume. The results of this study could be summarized as the following: the ballerina look is formed by ballet costume itself with being stick to or exposing human body line, or by casual dress mixed with a ballet costume; the materials consist of both the major materials for a ballet costume such as silk, chiffon, tulle, lace, or organza, and the usual materials for casual dress: a variety of color such as transparent, pastel etc. is also used with typically used colors like white and black: decoration is basically composed of ruffle, drape, gather and the distinctive accessory like toe shoes, ribbon tape, ankle warmer is used to show a feature of a ballet costume These formative characteristics of the ballerina look presented in modern fashion implies illusory, pure, and hybridity. traits. First, illusion of the ballerina look not only implicitly expresses a womans wish to experience a ballerina's fantastic world, but also recreates woman herself into a ballerina on a stage. Second, purity of the ballerina look makes design look feminine and smooth, which is expressed with girlish taste through materials and colors. Third, hybridity of the ballerina look suggests a standard of a new trend, which is active style, by adding comfortableness and flexibleness to romantic femininity.

Effect of Mo Addition on the Sinterability and Mechanical Properties of TiB$_2$-Fe Cermets (TiB$_2$-Fe 서메트의 소결성 및 기계적성질에 미치는 Mo첨가의 영향)

  • 최덕순
    • Journal of the Korean Ceramic Society
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    • v.36 no.5
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    • pp.471-477
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    • 1999
  • Fe and Fe-Mo binder were used to produce TiB2 based cermet by a pressureless sintering. The densification behaviour of TiB2-Fe-Mo cermet during liquid-phase sintering in argon was studied in relation to binder phase charactertics. The effects of Mo addition and sintering condition on the sintering behaviour and mechanical properties were also investigated. TiB2-based cermets with Fe-Mo binder composition showed a better sinterability than the cermets with only Fe binder. In TiB2-Fe-Mo cermet higher densities in the wide temperature range were obtained and also fully densified sintered cermet were obtained at 1873K The enhancement in the densification phenomenon of TiB2-Fe-Mo system can be explained by improved liquid phase wettability associated with the roles of Mo components as solute atoms. When Fe-Mo binders were used cermets with a finer grain size and enhanced mechanical properties wereproduced and new phases such as Fe2B and Mo2FeB2 were observed in the sintered cermet. The highest bending strength was obtained from the 20vol% Fe-Mo cermet and these hardness-fracture toughness combination in the wide binder compositions is better than that of TiB2-Fe cermet. In order to improve mechanical properties microstructure control with high purity powders is desirable because high purity powders prevent the formation of Fe2B and Mo2FeB2 phase which comsume the ductile binder phase.

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