• Proceedings of the Korean Society for Quality Management Conference
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• 1998.11a
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• pp.253-258
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• 1998

Staggered nested designs are the most popular class of unbalanced nested designs in practical fields. The most important features of the staggered nested design are that it has a very simple open-ended structure and each sum of squares in the analysis of variance has almost the same degrees of freedom. Based on the features, a class of unbalanced nested designs which is generalized of the staggered nested design is proposed. Some of the generalized staggered nested designs are shown to be more efficient than the staggered nested design in estimating some of variance components and their linear combinations.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.313-317
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• 2015

Due to the increase in incidence of infection of Mycobacterium tuberculosis complex (MTC), it is imperative that a rapid diagnosis accompanies the handling of MTC. This is due to the three to eight weeks it takes to culture Mycobacteria, and the lack of sensitivity of microscopic examination of AFB. Recently, nested PCR has been used to detect and diagnose mycobacteria. It is especially useful in complementing diagnosis by histological extra pulmonary. After culturing all the specimens and practicing the nested PCR, we did comparison analysis between nested PCR and culture. There were 76 specimens, 31 of which were positive. Of the 31 positive specimens in culturing, only 22 were positive in nested PCR. Of the 45 negative specimens, 36 were negative in nested PCR. As a result, Sensitivity was 71% and specificity was 80%. Furthermore, the positive predictive value was 71% and negative predictive value was 80%. These results indicate that nested PCR based techniques are sensitive, specific, and rapid methods for the detection of MTC.

• Journal of Korean Society on Water Environment
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• v.24 no.6
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• pp.817-822
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• 2008

In order to identify various Cryptosporidium species in environment, nested PCR-RFLP and DNA sequencing method were used. The sensitivity of nested PCR-RFLP based on 18s rRNA gene was shown to 1 oocyst. Therefore, we applied nested PCR-RFLP method to environmental samples. As a result, only 4 samples out of 8 samples confirmed as Cryptosporidium parvum by standard method of Cryptosporidium were identified as Cryptosporidium parvum by nested PCR-RFLP and DNA sequencing method. The rest of 4 samples among 8 samples were identified as Cryptosporidium muris, Cryptosporidium bailey. Therefore, in addition to standard method of Cryptosporidium, supplementary verification through nested PCR-RFLP and DNA sequencing should be needed to give more accurate information about risk of Cryptosporidium.

• Research in Plant Disease
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• v.11 no.1
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• pp.48-55
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• 2005

Cylindrocarpon destructans is a soil-borne plant pathogenic fungus causing root rot on ginseng and trees. Rapid and exact detection of this pathogen was practiced on ginseng seedlings by nested PCR using speciesspecific primer set. The second round of PCR amplification by Dest 1 and Dest 4 primer set formed 400 bp of species-specific fragment of C. destructans from the product of first round of amplification by ITS 1 and ITS 4 primer set. In the PCR sensitivity test based on DNA density, nested PCR detected to the limit of one fg and it meant the nested PCR could detect up to a few spores of C. destructans. Also, nested PCR made it possible to detect the pathogen from ginseng seedlings infected by replantation on artificial infested soil. Our nested PCR results using species-specific primer set could be utilized for diagnosis of root rot disease in ginseng cultivation.

• Research in Plant Disease
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• v.21 no.2
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• pp.74-81
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• 2015

The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation Sequencing of A. citrulli KACC10651, and the nested-PCR primer set (Ac-ORF 21F/Ac-ORF 21R) were selected by checking of specificity to A. citrulli with PCR assays. The selected nested-PCR primer amplified the 140 bp DNA only from A. citrulli strains, and detection sensitivity of the nested PCR increased 10,000 times of $1^{st}$ PCR detection limit (10 ng genomic DNA/PCR). The nested PCR detected A. citrulli from the all samples of seed surface wash (external seed detection) of the artificially inoculated watermelon seeds with $10^1cfu/ml$ and above population of A. citrulli while the nested PCR could not detected A. citrulli from the mashed seed suspension (internal seed detection) of the all artificially inoculated watermelon seeds. When the naturally infested watermelon seeds (10% seed infested rate with grow-out test) used, the nested PCR detected A. citrulli from 2 seed samples out of 10 replication samples externally and 5 seed samples out of 10 replication samples internally. We believe that the nested-PCR developed in this study will be useful method to detect A. citrulli from the Cucurbitaceae seeds.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.269-272
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• 2005

무선 네트워크 이동성 기술에 대한 연구가 수년간 진행되어 오면서 Mobile Network에 PAN(Personal Area Network)과 유사한 형태의 Nested Mobile Network에 대한 관심이 높아지고 있으며, 이러한 Nested Mobile Network에서의 경로최적화(Route Optimization : RO) 기술에 대한 연구가 활발하게 진행되고 있다. NEMO(NEtwork MObility)의 RO를 위해 제안된 논문 중에 ORC(Optimized Route Cache Protocol)에 대한 제안이 있었다.[1] NEMO Basic Support가 표준안으로 채택되면서 연구 대상에서 거론되지 않고 있지만, 복잡한 이동성 기술인 Nested Mobile Network상의 RO를 위해 다시 검토해 볼 수 있을 것이다. 또한 동일 저자에 의해 제안된 Nested Mobile Network 내부에 Ad-hoc Routing 알고리즘인 OLSR(Optimized Link State Routing Protocol)을 적용한 제안이 발표되었다.[2] 본 논문에서는 ORC와 Nested Mobile Network상의 OLSR Scheme을 적용하여 RO를 위한 방안을 제안하고자 한다.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.3-6
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• 2005

Mobile Network의 이동성에 대한 연구가 활발하게 진행되어 오면서, 이동네트워크의 복잡한 모델인 Nested Mobile Network에 대한 연구가 부각되고 있다. 이 Nested Mobile Network에 대한 연구에는 네트워크 내에 존재하는 노드와 외부의 CN(Correspondent Node)과의 패킷전송에서 경로최적화를 하기 위한 방법 등에 대한 연구가 진행되어 오고 있으며, Nested Mobile Network에서 Pinball routing 문제 등으로 인해 경로최적화가 이루어지지 못하고, 이 문제가 일반적인 Nested Mobile Network에서 패킷사이즈의 길이를 지나치게 길어지게 함으로써 전송지연을 발생시키는 것이다, 본 논문에서는 반복적인 CoA(Care-of-Address)의 치환과정을 통해 Nested Mobile Network 상의 Pinball routing 문제를 해결하기 위한 효율적인 방안을 제안하고자 한다.

• Journal of the Korean Data and Information Science Society
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• v.19 no.4
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• pp.1059-1071
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• 2008

In a balanced factorial design with a nested factor where crossed factors as well as a nested factor exist simultaneously, powers of the rank transformed FR statistic for testing the main, nested and interaction effects are superior to those of the parametric F statistic. In heavy tailed distributions such as exponential and double exponential distributions, powers of the FR statistic show much higher level than those of the F statistic. Further powers of the F and FR statistic for testing the main effect show the highest level in an absolute size as compared with powers of the F and FR statistic for testing the nested and interaction effects. However powers of the FR statistic for testing the nested and interaction effects rather than the main effect are greater in a relative size than powers of F statistic for the all population distributions.

• Journal of the Korean Statistical Society
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• v.29 no.1
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• pp.63-80
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• 2000

We consider the panel data regression model with nested error componets. In this paper, the several Lagrange Multipler tests for the nested error component model are derived. These tests extend the earlier work of Honda(1985), Moulton and Randolph(1989), Baltagi, et al.(1992) and King and Wu(1997) to the nested error component case. Monte Carlo experiments are conducted to study the performance of these LM tests.

• Journal of the Korean Statistical Society
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• v.30 no.4
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• pp.541-550
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• 2001

This paper considers a panel data regression model in which the disturbances follow a nested error components with serial correlation. Given this model, this paper derives several Lagrange Multiplier(LM) testis for the presence of serial correlation as well as random individual effects, nested effects, and for existence of serial correlation given random individual and nested effects.