• Fashion & Textile Research Journal
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• v.24 no.6
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• pp.801-811
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• 2022

In this study, we constructed a database of dyeing concentrations of all reactive dyes used for dyeing linen according to the thickness of the linen fabric and attempted to improve the reproducibility of the dyeing process for the commercialization of linen fabric materials. Linen fabric is a natural cellulose material that is comfortable and suitable for eco-friendly trends. It is a typical summer material with excellent breathability and thermal conductivity, quick moisture absorption and dissipation, and a cool touch. Dyeability and fastness were evaluated depending on the thickness of the linen fabric using a monochlorotriazine (MCT) reactive dye and bifunctional yellow, red, and blue dyes. All three colors of the MCT reactive dye and bifunctional dyes exhibited a darker shade as the fiber thickness of the marker increased. Fastness to washing was excellent at grades 4-5 or higher, regardless of the color of the dye and the number of linen fabrics. Although some color-fastness differences were noted, with the color change occurring most frequently with blue color, the fastness variations with dye type were mostly similar. The results of this study are expected to facilitate the selection of reactive dyes according to the thickness of the linen fabric based on the basic data from the laboratory, which will aid in the mass production of linen fabric and benefit the fashion industry.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.253-261
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• 2014

In this study, we investigated several chemical and physical characteristics of 4 weed seed fibers; Hemistepta lyrata (HEMLY), Imperata cylindrica var. koenigii (IMPCK), Metaplexis japonica (METJA) and Typha latifolia (TYPLA). In chemical composition, there were 74 (TYPLA)-88.5% (METJA) of holocellulose, 17 (IMPCK)-24% (METJA) of lignin, 0.22 (METJA)-4.2% (IMPCK) of ash, 2.2 (HEMLY)-7.8% (IMPCK) of hot water extractives and 0.4 (IMPCK)-6.3% (TYPLA) of solvent extractives. Alpha-cellulose proportion to holocellulose was similar among weed seed fibers as 45-48%. The crystallinity index (CI) of raw seed fibers was 53.2 (TYPLA)-65.9% (HEMLY). However, CI of the chemical treated fibers (EDA fibers) was a little increased and showed 61.1 (IMPCK)-71.8% (METJA). The maximum thermal decomposition temperature (MTDT) of the raw seed fibers were 312, 321.8, 331.5 and $341.6^{\circ}C$ in METJA, TYPLA, HEMLY and IMPCK, respectively. But the MTDT of the EDA fibers were 327, 327, 341.7 and $360.0^{\circ}C$ in HEMLY, TYPLA, METJA and IMPCK, respectively. Taken together, they showed a similar or better characteristics compared to the reported or commercial natural fiber resourses. Accordingly, they seem to be practically applicable as renewable resources for a new natural fibers.

• Applied Biological Chemistry
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• v.18 no.3
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• pp.109-116
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• 1975

In order to utilize the agricultural waste products for animal feeds, two high xylanase producing mold strains were selected from various sources of samples. The optimum conditions of xylanase production and the characteristics of the mold enzyme were investigated,and summarized as follows. 1. Two Aspergillus niger strains (experimental No. 1701 and 430) showed the high xylanase activity. 2. The highest xylanase production was obtained at pH 5.0-6.0 in two days. 3. Xylanase production in strain 1701 was increase with the addition of carboxy methyl cellulose, $NH_4H_2PO_4$ and corn steep liquor as carbon sources and natural nutrients, as respectively, while the other carbon, nitrogen, phosphate sources, natural nutrients and minerals gave no remarkable effect. In the strain 430, the enzyme procuction was not effected with the above substrate sources. 4. Maximum xylan hydrolysis reaction with the crude enzyme extract (33.3% v/v) was obtained in the 2% substrate concentration at pH 5.0 and $60^{\circ}C$ in three hours in both strains. 5. Maximum xylan hydrolysis rate was 95% at the optimum conditions for xylanase activity.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.477-485
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• 1998

To underatand in vitro regulation of differentiation, the effects of growth regulators and nitrogen source on metabolism of cell wall polysaccharides in suspension culture of kidney bean (Phaseolus vulgaris L.) were investigated. The suspension cells (cell clusters) were directly induced from the epicotyl segments of the seedlings, which were cultivated in MS medium supplemented with 1.0mg/L of 2,4-D and 0.5 mg/L of kinetin. When compared with cell wall sugar contents of the epicotyl segments, the cellulose content of the suspension-cultured cells decreased; while the pectin and hemicellulose content increased; suggesting increases of rhamnogalacturonan I and arabinogalactan IIduring the dedifferentiation, respectively, The effects of growth regulators(2,4-D, 1.0mg/L and kinetin, 0.5mg/L) and nitrogen source (potasium nitrate, 19.0mg/L and ammonium nitrate, 16.5 g/L) in the medium on the proliferation and the turnover of the cell wall polysaccharides were investigated for 30 days. In the medium with growth regulators and without nitrogen source, the proliferation rate was extremely high (16 folds). Growth regulators and nitrogen source increased the pectin content. Analysis of neutral sugar composition of pectin fraction showed that nitrogen source enhanced rhamnose level remarkably, suggesting that rhamnogalacturonan I was the one most likely synthesized. In hemicellulose fraction, growth regulators reduced arabinose level, suggesting that arabinogalactan II was degraded. And nitrogen source reduced galactose level, suggesting that xyloglucan was also degraded.

• The Korean Journal of Zoology
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• v.27 no.2
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• pp.103-116
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• 1984

The structural gene of rabbit hemoglobin was cloned into Pst I site of pBR322 in E. coli. The complementary DNA (cDNA) was synthesized from rabbit globin mRNA with avian myeloblastosis viral reverse transcriptase, and then RNA was destroyed at pH 11. The double stranded cDNA was synthesized with both Klenow fragment of E. coli DNA polymerase I and reverse transcriptase and then the hairpin loop was opened with Sl nuclease. Double stranded cDNA was subsequently tailed with dCTP and annealed to dGMP-tailed vector DNA. After transformation and initial screening of appropriate clones by plasmid size, the cloned colonies were identified by in situ colony hybridization using by plasmid size, the cloned colonies were identified by in situ colony hybridization using $[^32P]$-labeled cDNA probes and characterized the inserts with restriction endonucleases. The expression of cloned globin gene was investigated by standard radioimmunoassay using rat anti-rabbit Hb serum as primary antibody and goat antirat IgG serum as secondary antibody. The result suggested that the chimeric proteins (the part of $\\beta$-lactamase from the vector pBR322 and globin from rabbit) were supposedly produced in E. coli and the product had the antigenic determinant of rabbit hemoglobin.

• Journal of Life Science
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• v.29 no.3
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• pp.382-393
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• 2019

Wound care is a health industry concern affecting millions worldwide. Recent increase in metabolic disorders such as diabetes comes with elevated risk of wound-based complications. Treatment and management of wounds are difficult practices due to complexity of the wound healing process. Conventional wound dressings and treatment applications only provide limited benefits which are mainly aimed to keep wound protected from external factors. To improve wound care, recent developments make biopolymers to be of high interest and importance to researchers and medical practitioners. Biopolymers are polymers or natural origin produced by living organisms. They are credited to be highly biocompatible and biodegradable. Currently, studies reported biopolymers to exhibit various health beneficial properties such as antimicrobial, anti-inflammatory, hemostatic, cell proliferative and angiogenic activities which are crucial for effective wound management. Several biopolymers, namely chitosan, cellulose, collagen, hyaluronic acid and alginic acid have been already investigated and applied as wound dressing agents. Different derivatives of biopolymers have also been developed by cross-linking with other molecules, grafting with other polymers, and loading with bioactive agents or drugs which showed promising results towards wound healing without any undesired outcome such as scarring and physiological abnormalities. In this review, current applications of common biopolymers in wound treatment industry are highlighted to be a guide for further applications and studies.

• Korean Journal of Ecology and Environment
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• v.54 no.3
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• pp.199-208
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• 2021

Environmental DNA (eDNA) metabarcoding is effective method with high detection sensitivity for evaluating fish biodiversity and detecting endangered fish from natural water samples. We compared the richness of operational taxonomic units(OTUs) and composition of freshwater fishes according to filters(cellulose nitrate filter vs. glass fiber filter), extraction kits(DNeasy2^® Blood & Tissue Kit vs. DNeasy2^® PowerWater Kit), primer sets (12S rDNA vs. 16S rDNA), and PCR methods (conventional PCR vs. touchdown PCR) to determine the optimal conditions for metabarcoding analysis of Korean freshwater fish. The glass fiber filter and DNeasy2^® Blood & Tissue Kit combination showed the highest number of freshwater fish OTUs in both 12S and 16S rDNA. Among the four types, the primer sets only showed statistically significant difference in the average number of OTUs in class Actinopterygii (non-parametric Wilcoxon signed ranks test, p=0.005). However, there was no difference in the average number of OTUs in freshwater fish. The species composition also showed significant difference according to primer sets (PERMANOVA, Pseudo-F=6.9489, p=0.006), but no differences were observed in the other three types. The non-metric multidimensional scaling (NMDS) results revealed that species composition clustered together according to primer sets based on similarity of 65%; 16S rDNA primer set was mainly attributed to endangered species such as Microphysogobio koreensis and Pseudogobio brevicorpus. In contrast, the 12S rDNA primer set was mainly attributed to common species such as Zacco platypus and Coreoperca herzi. This study provides essential information on species diversity analysis using metabarcoding for environmental water samples obtained from rivers in Korea.

• Clinical and Experimental Reproductive Medicine
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• v.10 no.2
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• pp.1-11
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• 1983

${\beta}$-Glucuronidase from bull seminal plasma was partially purified by $(NH_4)_2SO_4$fractionation, two successive DEAE-cellulose columns, isoelectric focusing (pH 4 to 6) and Gel filtration on Sephadex G-200. Only one form of ${\beta}$-glucuronidase was obtained by isoelectric focusing at pH 5.13. Highly purified ${\beta}$-glucuronidase had specific activity of 34 units/mg protein and showed one major and some minor contaminants by disc gelk electrophoresis. The enzyme showed maximum activity at pH 5.2 and at $48^{\circ}C$. The enzyme was completely inhibited by 1,4 saccharo-${\alpha}$-lactone (5 mM). Albumin and 0.15 M NaCl increased the ${\beta}$-glucuronidase activity. Km of ${\beta}$-glucuronidase using phenolphthalein mono-${\beta}$-glucuronic acid as substrate was 2.9 mM and Vmax was $0.8{\mu}$mole/min. The enzyme appeared to be a glycoprotein by its binding to concanvalin·A. Rabbit and human sperm-acrosomal extracts and seminal plasma showed high ${\beta}$-glucuronidase activity.

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.156-164
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• 2013

Actinomycetes produce diverse secondary metabolites which have the primary importance in medicine, agriculture and food production, and key to this is their ability to interact with other organisms in natural habitats. In this study, we have investigated the taxonomical and functional diversity of actinomycetes in fecal sample of rhinoceros beetle larvae (Allomyrina dichotoma L.) by using culture-dependent and -independent approaches. For the culture-independent approach, the community DNA was extracted from the sample and 16S rRNA genes of actinomycetes were amplified using actinomycetes-specific PCR primers. Thirty-seven clones were classified into 15 genera and 24 species of actinomycetes. For the culture-dependent approach, 53 strains were isolated from larval feces, of which 27 isolates were selected based on morphological characteristics. The isolates were classified into 4 genera and 14 species, and 24 isolates (89%) were identified as the genus Streptomyces. Many of the representative isolates had antimicrobial activities against plant pathogenic fungi and Gram-positive bacteria. In addition, most of the isolates (78%) showed biochemical properties to hydrolyze cellulose and casein. The results demonstrated that diverse and valuable actinomycetes could be isolated from insect fecal samples, indicating that insect guts can be rich sources for novel bioactive compounds.

• Journal of the Korea Institute of Building Construction
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• v.16 no.3
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• pp.201-209
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• 2016

This study is to examine experimentally on the engineering properties of fiber reinforced concrete using kenaf(KN) fiber and another organic fibers for comparing test, and propose the usable method of KN fiber as an natural fiber in the concrete industry. It is to select 4 contents(0, 0.3, 0.6 and $0.9kg/m^3$) of KN fiber and 4 organic fibers (Jute, Cellulose, Polypropylene and Nylon). For this study, it is to perform various tests including slump, air content, plastic and drying shrinkage, flexural and tensile strength, carbonation depth for the fiber reinforced concrete according to contents of KN fiber and 4 organic fibers. The results of this study are as follows : In case of KN fiber contents $0.6kg/m^3$, it shows the effective results from increasing concrete strength including flexural and tensile, from decreasing plastic and drying shrinkage, carbonation depth. Also KN fiber is confirmed having excellent performances by comparing with test results of another organic fibers as same contents $0.6kg/m^3$. Therefore, considering concrete test results, cost and environment, KN fiber is proposed as the optimum contents in the range of $0.6kg/m^3$ and an effective fiber materials, and needs to keep up these study on the site application.