• 한국환경복원기술학회지
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• 제14권6호
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• pp.1-15
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• 2011

We recently witnessed increasingly more natural disturbances on forested land. On the other hand, only limited number of nursery grown tree species are available for restoration projects. At the same time, so little on the disturbance resistance of species has been studied that a selection of right species for right site condition becomes very difficult. This study is a compilation of the site specificities and disturbance resistance of each species native to South Korea. Each species was surveyed for 11 items related to site specificities, and 12 items related to disturbance resistance including the resistance to snow damage. This study was conducted for 161 tree species from 44 families : 21 evergreen conifer species, 2 deciduous conifer species, 18 evergreen broad-leaved species, 118 deciduous broad-leaved species, and 2 monocotyledon species. This study suggests that native species in South Korea show resistance to all types of natural disturbances except drought.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2000년도 제17차 정기총회 및 학술발표
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• pp.85-87
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• 2000

To obtain the genetic information of Korean native livestock, the karyotyping of Korean native chick were performed by high-resolution banding technique. The chromosomes were prepared from lymphocyte culture and early embryos with 200 Korean native chick which have been raised at National Livestock Research Institute. There were no significant difference between Korean native chick and Leghorn in the number of chromosomes and chromosomal morphological pattern. Using high resolution banding technique, the yield of G-bands of prophase is much greater than that can be obtained by International System for Standardzed Avian Karyotypes(ISSAK, 1999). The G-band landmarks of Korean native chick were similar to those of ISSAK and Leghorn except some macrochromosomes. chromosome Z and 3 had C-band variants with heteromorphic patterns on distal and centromeric site. The proportion of constitutive heterochromatin, the heterochromatin ratio of Korean native chick was significantly more than that of Leghorn in all chromosomes.

• 한국가금학회지
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• 제38권3호
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• pp.191-195
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• 2011

The objectives of this study are to identify specific functional genes which are related with growth and protein structure of the pectoral muscle of Korean native chicken. Pectoral muscle was isolated from three Korean native chickens (KNC, red brown, 12 months old, 2.41 ${\pm}$ 0.24 kg) and three Cornish chickens (16 month old, 2.76 ${\pm}$ 3.0 kg). The subtraction cDNA library was prepared in PCR4 Blunt-TOPO vector. The DNA sequence homology was compared with other breeds and species in GenBank. A clone NDS-81 was found to be unique for the DNA sequence homology with UBX family. Their partial sequence has high homology (98%) with chicken UBX domain D. Chicken UBX domain has chicken (93%), cattle (68%), dog (67%), mouse (64%) and, human (63%) nucleotide sequence homology. Several regions were mutated from T in chicken to C or G in the NDS-81 clone. The first site is LAD in chicken, but it was expressed as (L)RM in clone NDS-81. In this site, amino acids were changed from Ala to Arg, and from Asp to Met. The second site was changed from ER (Arg) in chicken to ED (Asp) in clone NDS-81. They are both containing functional side chains and play an important role in binding other proteins. Therefore, the clone NDS-81 could be a different candidate gene for the UBX family gene and could related with pectoral muscle structure of Korean native chicken.

• 한국환경생태학회지
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• 제24권4호
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• pp.493-499
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• 2010

자생 초본식물의 보존과 임간재배에 필요한 기초자료를 얻고자, 가리왕산과 중왕산 지역의 아고산대에 분포하는 천연활엽수림을 중심으로 주요 자생 초본식물의 분포와 입지인자를 조사하고 상호관련성을 분석하였다. 초본식물 분포조사에서는 136종의 초본식물이 확인되었으며, 개별꽃의 상대우점치가 10.8%로 가장 높게 나타났다. 그 밖에 주요 식물종은 벌깨덩굴, 현호색, 참나물, 대사초, 단풍취, 큰개별꽃, 얼레지, 곰취, 만주족도리풀, 피나물, 노루오줌 등 이었다. 노루귀와 단풍취; 참나물과 두메투구꽃; 두메투구꽃과 풀솜대; 벌깨덩굴과 큰개별꽃, 등의 종간에는 매우 높은 정의 상관이 인정되었다. 주요 식물종의 유집분석 결과, 7개의 그룹으로 나뉘어졌다. 주요 자원식물종이 선호하는 입지인자들이 밝혀졌다. 곰취, 단풍취, 박쥐나물, 얼레지, 참나물 등은 해발고가 높을수록 많이 분포하였고, 곰취는 토심이 깊을수록 많이 분포하는 것으로 나타났다. 얻어진 결과는 평창군 아고산 천연활엽수림의 보전과 주요 지원식물의 임간재배에 활용할 수 있을 것이라 사료된다.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1325-1329
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• 2006

Structural modeling of $\beta$-galactosidase from L. lactis ssp. lactis 7962 has shown that the residues Cys-472 and His-509 are located in the wall of the active-site cavity. To examine the functions of Cys-472 and His-509, we generated five site-specific mutants: Cys-472-Ser, Cys-472-Thr, Cys-472-Met, His-509-Asn, and His-509-Phe. $\beta$-Galactosidase substituted at Cys-472 with Met or His-509 with Phe had <3% of the activity of the native enzyme when assayed using ONPG as substrate. The other mutants Cys-472-Ser, Cys-472-Thr, and His-509-Asn had ca. 10-15% of the native enzyme activity. The V$_max$ values of the five mutated enzymes were lower (60-7,000-fold) than that of native enzyme. These results show that the catalytic ability of $\beta$-galactosidase is significantly affected by mutations at Cys-472 or His-509.

• Molecules and Cells
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• 제20권3호
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• pp.325-330
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• 2005

Ancient cattle bones were excavated from archaeological sites in Jeju, Korea. We used molecular genetic techniques to identify the species and establish its relationship to extant cattle breeds. Ancient DNA was extracted from four sources: a humerus (Gonae site, A.D. 700-800), two fragments of radius, and a tooth (Kwakji site, A.D. 0-900). The mitochondrial DNA (mtDNA) D-loop regions were cloned, sequenced, and compared with previously reported sequences of various cattle breeds (9 Asian, 8 European, and 3 African). The results revealed that these bones were of the breed, Bos taurus, and a phylogenetic tree indicated that the four cattle bones formed a monophyletic group with Jeju native black cattle. However, the patterns of sequence variation and reports from archaeological sites suggest that a few wild cattle, with a different maternal lineage, may have existed on Jeju Island. Our results will contribute to further studies of the origin of Jeju native cattle and the possible existence of local wild cattle.

• 한국가축번식학회지
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• 제8권2호
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• pp.87-91
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• 1984

This study was conducted to determine the optimum time and the insemination site of frozen in Cheju native cows, and 660 cows first service inseminated with rectovaginal methods by 3 skillful technicians as 3 districts near Cheju City and Sogwipo City areas obtained the following results. Optimum time for insemination was 16-20 hours after the onset of estrus and 80.6% of 67 conceived to first insemination. In the rectovaginal technique used widely at present conception rate for the palpation of ovaries showed 6.6% (index) higher than that of non-palpation. Conception rate of the insemination site; the intra-uterine horn, intra-uterus, just inside the cervix and depositing in mid-cervix were 62.5, 78.4, 74.6, and 80.9% respectively, however, there was no difference in conception rate between cervical and uterine deposition of forzon semen except for the intra-uterine horn.

• 한국임상수의학회지
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• 제16권2호
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• pp.486-491
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• 1999

One day to one week-old 3 female Korean native calves were referred to the Veterinary Teaching Hospital, Chonbuk National University, with atresia ani. The authors performed radiographic and ultrasonographic diagnosis with physical examination, general hematology and blood chemical examination as investigation. The atresia ani were type I, II, III in each 3 calves. In abdominal radiography and ultrasonography, there were ventral displacement of descending colon(gas and feces filled) and typical enlargement of the blind end of colon in all cases. Operation site was determined by radiographic and ultrasonographic findings in these cases. Surgical treatment, two cases were translocation of the colon to the body wall exiting as a colostomy and one case was corrected by making a circumcision through the skin covering the site of the anus.

• 한국산림과학회지
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• 제87권3호
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• pp.459-465
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• 1998

이 연구(硏究)는 자생(自生) 초본식물(草本植物)의 보존(保存)과 임한재배(林閒裁培)에 필요한 기초자료(基礎資料)를 얻고자, 오대산(烏臺山)의 동대산, 두로봉, 상왕봉, 비로봉을 잇는 아고산대(亞高山帶)를 중심으로 자생(自生)하는 초본식물(草本植物)의 분포(分布)와 입지인자(立地因子)를 조사(調査)하고 상호관련성(相互關聯性) 분석(分析)하였다. 단풍취, 큰개별꽃 및 참나물은 관엽수혼효림(關葉樹混淆林)에 주로 분포(分布)하며, 요강나물, 대사초, 오리방풀 및 나비나물은 갭에 주로 분포(分布)하는 것으로 나타났다. 사면(斜面) 방위별(方位別) 분석(分析)에서는 곰취, 진범, 박쥐나물 및 넓은잎외잎쑥은 유의차(有意差)가 인정(認定)되었다. 넓은잎외잎쑥은 남향 사면에 많이 분포(分布)하며, 곰취, 진범 및 박쥐나물은 북향이나 서향에서 많이 분포(分布)하였다. 토양수분(土壤水分) 조건별(條件別) 분석(分析)에서는 진범과 넓은잎외잎쑥에서 유의차(有意差)가 인정(認定)되었다. 넓은잎외잎쑥은 건조한 입지(立地)에 많이 분포(分布)하며, 진범은 토양수분 조건이 좋은 곳에 많이 분포(分布)하였다. 수관울폐도(樹冠鬱閉度)와의 상관관계(相關關係)에서는 요강나물, 대사초, 오리방풀, 나비나물 등이 부(負)의 상관(相關)을 보였다. 진범, 참나물, 벌깨덩굴, 풀솜대 및 큰개별꽃 등은 다소 습한 숲그늘을 선호(選好)하고, 송이풀, 요강나물 및 넓은잎외잎쑥 등은 햇빛이 잘 드는 숲 가장자리나 개활지(開豁地)를 선호(選好)하는 것으로 나타났다.