• The Journal of the Korea Contents Association
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• v.10 no.11
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• pp.68-79
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• 2010

With the social infrastructure being deteriorated, there is a growing need to introduce the asset management to social infrastructure management in order to increase their value and save budget. Social infrastructure asset management is a new concept of facility management in response to these demands. It is defined as a procedure for collecting and analysing facility maintenance data and for making and practicing an economically optimized management plan. Detailed survey work of asset management business is analyzed in order to derive a strategy for asset management information. The contents of IIMM of New Zealand and the asset management definition of the FHWA of the United States, and representative facility management systems of Korea are analysed. The role between organizations and the relationship between business and organization were analyzed. Information required for asset management and for existing facility management systems is compared with business of asset management. In this thesis, three development strategies are suggested. The first one is to develop core business of asset management while excluding duplicated development. The second one is to divide system's structure into three layers. And the last one is to share information by interfacing asset management systems with existing facility management systems.

• Journal of Ginseng Research
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• v.35 no.4
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• pp.471-478
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• 2011

Ginseng, the root of Panax ginseng, is one of the oldest herbal medicines. It has a variety of physiological and pharmacological effects. Recently, we isolated a subset of glycolipoproteins that we designated gintonin, and demonstrated that it induced transient change in intracellular calcium concentration $([Ca^{2+}]_i)$ in cells via G-protein-coupled receptor signaling pathway(s). The previous method for gintonin isolation included multiple steps using methanol, butanol, and other organic solvents. In the present study, we developed a much simple method for the preparation of gintonin from ginseng root using 80% ethanol extraction. The extracted fraction was designated edible gintonin. This method produced a high yield of gintonin (0.20%). The chemical characteristics of gintonin such as molecular weight and the composition of the extract product were almost identical as the gintonin prepared using the previous extraction regimen involving various organic solvents. We also examined the physiological effects of edible gintonin on endogenous $Ca^{2+}$-activated $Cl^-$ channel activity of Xenopus oocytes. The 50% effective dose was $1.03{\pm}0.3\;{\mu}g$/mL. Finally, since gintonin preparation through ethanol extraction is easily reproducible, gintonin could be commercially applied for ginseng-derived functional health food and/or drug following the confirmations of in vitro and in vivo physiological and pharmacological effects of gintonin.

• The Journal of the Korea Contents Association
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• v.12 no.1
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• pp.68-79
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• 2012

Korea was followed by a rapid increase in road construction investment in late 1980s with the help of strong economic conditions. As a result of such investment, it will be expected that there must be a rapid increase in demand for maintenance or rehabilitation of the facilities after 2010. We are developing asset management information systems for enhancing user's satisfaction and innovating the facility management techniques within a limited budget. We suggested the assessment items for the Level of Service for tunnel facility's operation and maintenance(O&M), in this study. We have surveyed for the importance of assessment items by considering both user's viewpoints and facility management agency's viewpoints. We came to realize the relative importance of the items based on this survey using the Analytic Hierarchy Process(AHP) method proposed by Satty. The relative importance of assessment items in terms of the user's viewpoint was identified in descending order such as safety, mobility, accessibility, convenience, O&M efficiency, comfort, rural revitalization, environment, and information. Also, the importance of the items in terms of the management agency's viewpoint was identified in descending order such as safety, mobility, accessibility, environment, rural revitalization, convenience, O&M efficiency, information, and comfort. It means that safety of tunnel facilities is more important than mobility, although which is the basic function of the road facility, both in agency's and user's concern. Therefore, the agencies have to pay more attentions and efforts to ensure safety of tunnel facilities when they establish the investment plans for the tunnel facility O&M.

• Korean Journal of Veterinary Service
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• v.41 no.1
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• pp.29-39
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• 2018

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

• Korean Journal of Poultry Science
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• v.38 no.2
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• pp.89-96
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• 2011

The outbreak of Newcastle disease occurred for the first time at a commercial chicken farm near Ulaanbaatar, Mongolia in August 2010. Newcastle disease virus (NDV) obtained from infected chickens in Mongolia was characterized by biological and molecular biological approches. Mongolian NDV isolate killed all of chicken embryos within 60 h in the mean death time assay, indicating virulent for chicken. A genomic region of 695 nts between nts 1055 of the M gene and 508 of the F gene was amplified by RT-PCR and sequenced. The deduced amino acid sequence of the F protein cleavage site was $^{112}RRQKRF^{117}$, which is a typical sequence of velogenic strains of NDV and is agreement with the result of the MDT assay. The sequence of the partial F gene (nts 47 to 435) was used for genotyping by phylogenetic analysis. The phylogenetic analysis showed that the Mongolian isolate was of genotype VII within class II of NDV. Further phylogenetic analysis on the genotype VII strains revealed that the isolates placed in a genetic sublineage of VIId and most closely related with velogenic strains of NDV circulating in Far-east Asian region especially China, suggesting the introduction of velogenic NDV into Mongolia from neighboring countries.

• Journal of Ginseng Research
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• v.34 no.3
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• pp.246-253
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• 2010

Ginsenoside $Rg_3$ ($Rg_3$), one of the active ingredients in Panax ginseng, attenuates NMDA receptor-mediated currents in vitro and antagonizes NMDA receptors through a glycine modulatory site in rat cultured hippocampal neurons. In the present study, we examined the neuroprotective effects of $Rg_3$ on 24-hydroxycholesterol (24-OH-chol)-induced cytotoxicity in vitro. The results showed that $Rg_3$ treatment significantly and dose-dependently inhibited 24-OH-chol-induced cell death in rat cultured cortical neurons, with an $IC_{50}$ value of $28.7{\pm}7.5\;{\mu}m$. Furthermore, the $Rg_3$ treatment not only significantly reduced DNA damage, but also dose-dependently attenuated 24-OH-chol-induced caspase-3 activity. To study the mechanisms underlying the in vitro neuroprotective effects of $Rg_3$ against 25-OH-chol-induced cytotoxicity, we also examined the effect of $Rg_3$ on intracellular $Ca^{2+}$ elevations in cultured neurons and found that $Rg_3$ treatment dose-dependently inhibited increases in intracellular $Ca^{2+}$, with an $IC_{50}$ value of $40.37{\pm}12.88\;{\mu}m$. Additionally, $Rg_3$ treatment dose-dependently inhibited apoptosis with an $IC_{50}$ of $47.3{\pm}14.2\;{\mu}m$. Finally, after confirming the protective effect of $Rg_3$ using a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay, we found that $Rg_3$ is an active component in ginseng-mediated neuroprotection. These results collectively indicate that $Rg_3$-induced neuroprotection against 24-OH-chol in rat cortical neurons might be achieved via inhibition of a 24-OH-chol-mediated $Ca^{2+}$ channel. This is the first report to employ cortical neurons to study the neuroprotective effects of $Rg_3$ against 24-OH-chol. In conclusion, $Rg_3$ was effective for protecting cells against 24-OH-chol-induced cytotoxicity in rat cortical neurons. This protective ability makes $Rg_3$ a promising agent in pathologies implicating neurodegeneration such as apoptosis or neuronal cell death.

• Journal of Digital Convergence
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• v.12 no.10
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• pp.201-209
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• 2014

The purpose of this study is to analyze subjective and objective factors for the successful operation of One-Person Creative Firms and find significant variables in accordance with the sales and net profit that are representing business performances. Additionally, we were trying to find that what One-Person Creative Firms have had a government assistance need the supports in order to achieving a good performance in sales and net profit. In the result of this study, the sales volume is related that two(2) subjective(Judgment, Planning) and four(4) objective factors(Patent Application, Patent Registration, Business Duration, Initial Capital). The net profit is related that Challenging Spirits in subjective factors and Run-up to the Business, Business Duration, Initial Capital in objective factors. Ultimately, these factors are important variables for the sales and net profit in relationship both the positive(+) and negative(-). This is an exploratory nature of the study rather than the rigorous hypothesis testing. Therefore, a rigorous hypothesis test study model to derive a more detailed study is needed based on the results of this study in the future.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.3
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• pp.233-240
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• 1994

This study was conducted to compare the endocrine milieu, and pregnancy rates in In Vitro Fertilization and Embryo Transfer(IVF-ET) program employing combined with gonadotropin releasing hormone agonist(GnRH-a) and pergonal(LH 75lU+FSH 75lU) when either human chorionic gonadotropin(HCG) or progesterone were used for luteal phase support. A total number of 40 IVF-ET treatment cycles were prospectively studied. Ovarian hyperstimulation method was modified ultrashort protocol using GnRH-a. All patients started Decapeptyl at menstrual cycle day # 2, and HMG was started at # 3 days. When leading follicle was ${\geqq}$18mm or at least two follicles were ${\geqq}$14mm in diameter, HCG 10000lU intramuscularly was injected. After 36 hours HCG administration, oocytes were retrieved as usual guided by transvaginal ultrasound. Embryo were transfered 36-48 hours later. The patient's cycles were prospectively randomized to receive HCG(20cycles) or Progesterone (20cycles) for luteal support. The progesterone group received 25mg 1M starting from the day of ET. The HCG group received 1500IU 1M. on days 0, +2, +5 after ET. Estadiol($E_2$) and Progesterone($P_4$) were measured on the day of oocyte aspiration, ET day, and every 6 days thereafter. Results were follows as; 1. Estradiol, progesterone and LH levels on the day of HCG trigger, retrieved oocytes and number of transfered embryo were not significantly different in both groups. 2. On the day of aspiration and embryo transfered day, $E_2$, $P_4$ level were significantly higher in progesterone group than HCG group(p<0.01). 3. $E_2$, $P_4$ level on 6 days after ET were significantly higher in progesterone group than HCG group(p<0.01). But, $P_4/E_2$ ratio was not different in both groups. 4. $E_2$, $P_4$ level 12 days after ET were decreased abruptly in both groups and higher hormonal level appeared in HCG group(P<0.01). 5. The total pregnancy rate in the HCG group was 40% (8/20) and in the progesterone group 15%(3/20). 6. Comparing the pregnant and nonpregnant cases progesterone group was not different the hormonal status. In HCG group, pregnant cases appeared in higher $P_4$, $P_4/E_2$ ratio than nonpregnanct cases(P<0.01).

• Korean Journal of Materials Research
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• v.12 no.9
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• pp.750-760
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• 2002

In this study, three solders, Sn-37Pb, Sn-3.5Ag, and Sn-3.8Ag-0.7Cu were screen printed on both electroless Ni/Au and OSP metal finished micro-via PCBs (Printed Circuit Boards). The interfacial reaction between PCB metal pad finish materials and solder materials, and its effects on the solder bump joint mechanical reliability were investigated. The lead free solders formed a large amount of intermetallic compounds (IMC) than Sn-37Pb on both electroless Ni/Au and OSP (Organic Solderabilty Preservatives) finished PCBs during solder reflows because of the higher Sn content and higher reflow temperature. For OSP finish, scallop-like $Cu_{6}$ /$Sn_{5}$ and planar $Cu_3$Sn intermetallic compounds (IMC) were formed, and fracture occurred 100% within the solder regardless of reflow numbers and solder materials. Bump shear strength of lead free solders showed higher value than that of Sn-37Pb solder, because lead free solders are usually harder than eutectic Sn-37Pb solder. For Ni/Au finish, polygonal shaped $Ni_3$$Sn_4$ IMC and P-rich Ni layer were formed, and a brittle fracture at the Ni-Sn IMC layer or the interface between Ni-Sn intermetallic and P-rich Ni layer was observed after several reflows. Therefore, bump shear strength values of the Ni/Au finish are relatively lower than those of OSP finish. Especially, spalled IMCs at Sn-3.5Ag interface was observed after several reflow times. And, for the Sn-3.8Ag-0.7Cu solder case, the ternary Sn-Ni-Cu IMCs were observed. As a result, it was found that OSP finished PCB was a better choice for solders on PCB in terms of flip chip mechanical reliability.

• Journal of Oriental Neuropsychiatry
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• v.30 no.3
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• pp.165-175
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• 2019

Objectives: This study was conducted to investigate the emotional characteristics of the patients within one year after stroke using the Core Seven Emotions Inventory-Short Form. Methods: It analyzed the medical records of 39 stroke patients who had visited W University hospital and completed the Core Seven-Emotions Inventory-Short Form (CSEI-S), the K-MBI, the PWI-SF, and the HADS. Patients data were analyzed by descriptive analysis, frequency analysis, the Mann-Whitney U test, and the nonparametric test of the CSEI according to the period of stroke onset in patients and Spearman's correlation analysis using the SPSS (Statistical Package for the Social Science, IBM, United States of America) Version 23.0. Results: First, in the case of depression, all subjects were in the danger group, although more than half of the joy, anger, thinking, sadness and fear were included in the normal group in the shortening of the key seven-point scale. Secondly, in measuring the social and psychological stress and hospital anxiety and depression, social and psychological stress were indicated to be a potential danger group, while anxiety and depression were shown to be a healthy group. Thirdly, the emotional characteristics were explored according to the sub-factor of the shortening of the key seven-figure emotional scale after being classified within two months, between two and six months and over six months depending on the duration of the outbreak. As a result, statistically significant, Joy was the highest score over six months and the lowest score between two and six months. On the other hand, the case of depression was depicted to be significantly lower at over six months, and was highest between two and six months. Finally, the correlations on each scale were statistically significant. Conclusions: This study provided that the CSEI-S can be used to simply to measure the emotions of patients according to the period of the onset of stroke in the clinical scene.