Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the rapid detection of foot-and-mouth disease virus

구제역바이러스 신속진단을 위한 pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) 진단법

  • Lim, Da-Rae (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Yu-Ri (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Sun-Young (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Kim, Hye-Ryung (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Min-Ji (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Ku, Bok-Kyung (Foot-and-Mouth Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Nah, Jin-Ju (Foot-and-Mouth Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Ryoo, So-Yoon (Foot-and-Mouth Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Wee, Sung-Hwan (Foot-and-Mouth Disease Research Division, Animal and Plant Quarantine Agency) ;
  • Jeon, Hyo-Sung (M Monitor Inc.) ;
  • Kim, Ji-Jeong (M Monitor Inc.) ;
  • Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, Hyeong-Woo (Institute of Research and Development, Scorpiogen Co., Hankyong National University) ;
  • Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)
  • 임다래 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박유리 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박선영 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 김혜령 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 박민지 (경북대학교 수의과대학 & 수의전염병제어센터) ;
  • 구복경 (농림축산검역본부 구제역진단과) ;
  • 나진주 (농림축산검역본부 구제역진단과) ;
  • 유소윤 (농림축산검역본부 구제역진단과) ;
  • 위성환 (농림축산검역본부 구제역진단과) ;
  • 전효성 ((주)엠모니터) ;
  • 김지정 ((주)엠모니터) ;
  • 전보영 (연세대학교 보건과학대학 임상병리학과) ;
  • 이형우 (스콜피오진(주)) ;
  • 박최규 (경북대학교 수의과대학 & 수의전염병제어센터)
  • Received : 2017.12.08
  • Accepted : 2018.01.24
  • Published : 2018.03.30
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Abstract

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

Keywords

References

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