• 한국산림과학회지
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• 제79권3호
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• pp.316-321
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• 1990

본(本) 실험(實驗)은 송피함량(松皮含量)을 달리한 배양토(培養土) 0, 25, 50, 75%에 균근균(菌根菌)을 접종(接種)하여 처리(處理)에 따른 균근형성(菌根形成) 및 식물생장(植物生長)에 미치는 영향(影響)을 구명(究明)코자 실시(實施)되었으며 결과(結果)는 다음과 같다. 1. 균근(菌根) 접종묘(接種苗)가 비접종묘(非接種苗)에 비(比)해 세근(細根), 균근수(菌根數), 개체생장율(個體生長率), 엽면적(葉面積) 및 묘고(苗高)가 증가(增加)하였다. 2. 송피함량(松皮含量)에 따른 균근형성율(菌根形成率)은 25%구(區)가 높았으며 50% 및 70%구(區)에서는 감소(減少)하였다. 3. 동일송피(同一松皮) 함량구(含量區)에서는 균근접종구(菌根接種區)가 무접종구(無接種區)보다 묘고(苗高) 및 엽(葉), 경(莖), 근건중(根乾重)이 증가(增加)하였다.

• 한국산림과학회지
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• 제77권4호
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• pp.361-370
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• 1988

본(本) 연구(硏究)는 해송묘목(海松苗木)에 모래밭버섯(Pisolithus tinctorius) 균(菌)을 인공(人工) 접종(接種)한 후(後) 두개의 혼합토(混合土)와 6개의 질소수준(窒素水準)으로 처리(處理)된 실생묘(實生苗)의 생장(生長)과 균근(菌根) 형성(形成) 그리고 양료흡수관계(養料吸收關係)를 추정(推定)키 위(爲)해 실시(實施)되었다. 1) $50-150{\mu}g/ml$ N로 시비(施肥)된 vermiculite에서 자란 실험묘(實生苗)는 pinnate type과 cluster-like type이 잘 발달(發達)하였으나, 사질괴토(砂質壞土)는 monopodial, cluster-like type 그리고 pinnate type 이 발달(發達)하였다. 2) 균근형성(菌根形成)의 과정시비수준(適正施肥水準)은 50 또는 $150{\mu}g/ml$로 균근형성(菌根形成)이 각각(各各) $86.4({\pm}3.14)%$%와 $73.0({\pm}7.21)%$였다. 그러나 질소수준(窒素水準)이 증가(增加)하면 균근형성(菌根形成)이 감소(減少)하였다. 3) $450{\mu}g/ml$N로 시비(施肥)된 실생묘(實生苗)는 순(純) 회수율(同化率)(NAR)과 개체생장율(個體生長率)(CGR)이 생장초기(生長初期)에는 감소(減少)하였으나 질소시비(窒素施肥)가 멈춘 8월(月) 이후(以後)에는 다시 증가(增加)하였다. 4) 실생묘(實生苗)의 무기양료(無機養料) 흡수(吸收)는 질괴토(砂質壞土) 및 무접종구(無接種區)에 비해 vermiculite와 균근처리구(菌根處理區)에서 증가(增加)하였다. 그리고 회수율(回收率)은 $350{\mu}g/ml$ 질소수준(窒素水準)까지는 질소시비(窒素施肥) 증가(增加)에 따라 점진적(漸進的)으로 증가(增加)하였으나 $450{\mu}g/ml$ 질소수준(窒素水準)에서는 도리어 흡수(吸收)가 제한(制限)되었다.

• 한국환경복원기술학회지
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• 제5권2호
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• pp.53-62
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• 2002

The objectives of this study were to understand the tolerance mechanism of woody plants to water stress and tolerance changes in relation to mycorrhizal formation. Lespedeza cyrtobotrya Miq. commonly used for erosion control in slopes were raised from seeds and transplanted to 120 plastic pots. Sixty pots received the top soil of a Fraxinus americana forest, while remaining 60 pots received the autoclaved top soil. The forest soil contained 1,200 spores per 100g of arbuscular endomycorrhizal fungus, mostly Glomus sp. The plants were raised outside with regular supply of water and mineral nutrients. Two kinds of water deficit treatment and a control were started at the middle of July : cyclic water deficit treatment with 3 cycles of sequential water stress at the point of xylem water potential of about -0.6, -0.6, and -1.7 MPa and recovery, and non-cyclic water deficit treatment with single water stress at about -1.5 MPa. The non-stressed plants received plenty of water throughout the period. In late August the plants were harvested for measurements of dry weight, N, P, carbohydrate contents, net photosynthesis and superoxide dismutase(SOD) activities. Both cyclic and non-cyclic water deficit treatments reduced dry weight by 60% and 40%, respectively, and reduced nitrogen absorption, while increased SOD activities. Water-stressed plants also showed increased carbohydrate contents in the leaves and lowered stomatal conductance. Mycorrhizal inoculation resulted in an average of 40% infection of roots and 2-3 times increase in P absorption in water-stressed as well as non-stressed plants. Mycorrhizal formation also increased shoot-root ratio. The results that SOD activities of water-stressed plants with mycorrhizal infection were significantly lower than those of non-mycorrhizal plants suggest the possibility of improvement of water-stressed condition by mycorrhizal formation. It was concluded that endomycorrhizal formation increased tolerance of Lespedeza cyrtobotrya seedlings to water stress.

• 한국산림과학회지
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• 제59권1호
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• pp.121-142
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• 1983

Recently mycorrhizal research has been one of the most fast-growing research areas in modern plant science and microbiology. The application potential of mycorrhizal techniques to agriculture and forestry is enormous in view of the ubiquitous nature of mycorrhizae and known benefits of mycorrhizae to host plants. Unfortunately, very few scientists in Korea are currently involved in mycorrhizal research. When a team of American plant pathologists visited Korea in September 1982 to participate in the Korea-U.S.A. Joint Seminar on Forest Diseases and Insect Pests, they were surprised by the principal author's statement that there was no single research project on mycorrhizae sponsored by Korean government or any scientific institutions. The author initiated a few years ago a research project on the ecology of tree mycorrhizae with a foreign financial support. Major areas of interest were survey of ectomycorrhizae in relation to soil fertility, taxonomic distribution of mycorrhizae among woody plants, identification of ectomycorrhizal fungi, and growth response of woody plants to artificial inoculation. In spite of the enormous application potential of mycorrhizae to agronomic plants, the subject of mycorrhizae has not been recognized by Korean agronomists, foresters or pathologists. The purpose of this review rather written in Korean is to introduce the techniques of mycorrhizal research to Korean scientists and to urge them to participate in challenging new scientific field which might bring us a remarkable increase in crop productivity and tree growth through manipulation of this unique symbiosis. In this review, following topics were discussed in the same order: introduction; brief history of mycorrhizal research; morphology and classification of mycorrhizae; distribution of mycorrhizae in plant kingdom and in soil profile; physiology of mycorrhizae (functions, mineral nutrition, mycorrhizal formation); interaction of mycorrhizae with soil-born plant pathogens. mycorrhizae in nitrogen-fixing plants; application of mycorrhizal techniques to nursery practices (isolation, culture, inoculation, and response); prospect in the future.

• 한국산림과학회지
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• 제94권3호통권160호
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• pp.146-152
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• 2005

훼손된 비탈면의 조림에 균근균의 이용가능성을 타진하기 위하여, 땅찌만가닥버섯(Lyophyllum shimeji)의 배양학적 특성 및 인공접종에 의한 참싸리(Lespedeza cyrtobotrya)에서의 균근 형성 특성을 조사하였다. L. shimeji는 MP (1% malt extract, 0.1% peptone, 1% glucose, 1.5% agar)배지에서 균사 생장이 가장 왕성하였으며, 균사 최적 배양 온도 및 pH는 각각 $25^{\circ}C$ 및 pH 6 이었다. 인공접종에 의한 참싸리에서의 균근 형성 특성을 조사한 결과, L. shimeji는 Hartig net을 형성하는 외생균근의 특성을 나타내었으며, 균근 접종 실생 묘목 뿌리의 생장율은 대조구에 비하여 우수하였다. 균근 접종 실생 묘목을 비탈면에 이식하였을 때의 생존율 및 건중량은 각각 62% 및 850 mg/본 이었으나, 균근을 접종하지 않은 실생 묘목의 생존율 및 건중량은 각각 11% 및 430 mg/본 이었다.

• 한국토양비료학회지
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• 제36권5호
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• pp.311-322
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• 2003

공생균근은 식물 뿌리에 의한 인산 등 무기염류의 흡수를 촉진시키지만 토양의 인산 농도 가 너무 낮거나 높으면 공생균근 형성이 억제되는 것으로 알려져 있다. 본 연구는 화산회토양에서 감귤 뿌리의 공생균근 형성에 적합한 유효인산 수준을 구명하기 위하여 수행하였다. 중과석을 인 공급원으로 하여 인산 시용량을 $0-1000mg\;kg^{-1}$으로 달리한 토양에서 공생균근균 접종과 비접종 처리로 나누어 탱자 유묘를 화분에 심어 유리온실에서 5개월 동안 재배하였다. 탱자 유묘의 공생균근 형성율은 인산 무시용구(유효인산 함량 $0.73mg\;kg^{-1}$)에서 33%이었는데, $50mg\;kg^{-1}$ 시용구(유효인산 함량 $2.6mg\;kg^{-1}$)에서 43%로 증가되었지만 $200mg\;kg^{-1}$ 시용구(유효인산 함량 $8.3mg\;kg^{-1}$)에서는 34%로 무시용구와 비슷하였고 그 이상 인산 시용량이 증가함에 따라 형성율은 오히려 감소하였다. 공생균근균의 접종으로 초장, 생체중 및 건물중 등으로 측정된 탱자 유묘의 생장은 비접종 처리구와 비교하여 모든 인산 처리 수준에서 증가되었으며, 그 증가량은 공생균근 형성율에 비례하였다. 그리고 탱자 유묘의 인산을 비롯한 무기양분 흡수량도 모든 인산 처리 수준에서 공생균근 형성율과 높은 정의 상관관계를 보였다.

• Mycobiology
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• 제33권1호
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• pp.41-50
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• 2005

Results from an innovative approach to improve remediation in the rhizosphere by encouraging healthy plant growth and thus enhancing microbial activity are reported. The effect of arbuscular mycorrhizal fungi (Am) on remediation efficacy of wheat, mungbean and eggplant grown in soil spiked with polyaromatic hydrocarbons (PAH) was assessed in a pot experiment. The results of this study showed that Am inoculation enhanced dissipation amount of PAHs in planted soil, plant uptake PAHs, dissipation amount of PAHs in planted versus unplanted spiked soil and loss of PAHs by the plant-promoted biodegradation. A number of parameters were monitored including plant shoot and root dry weight, plant tissue water content, plant chlorophyll, root lipid content, oxido-reductase enzyme activities in plant and soil rhizosphere and total microbial count in the rhizospheric soil. The observed physiological data indicate that plant growth and tolerance increased with Am, but reduced by PAH. This was reflected by levels of mycorrhizal root colonization which were higher for mungbean, moderate for wheat and low for eggplant. Levels of Am colonization increased on mungbean > wheat > eggplant. This is consistent with the efficacy of plant in dissipation of PAHs in spiked soil. Highly significant positive correlations were shown between of arbuscular formation in root segments (A)) and plant water content, root lipids, peroxidase, catalase polyphenol oxidase and total microbial count in soil rhizosphere as well as PAH dissipation in spiked soil. As consequence of the treatment with Am, the plants provide a greater sink for the contaminants since they are better able to survive and grow.

• 한국산림과학회지
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• 제79권3호
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• pp.309-315
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• 1990

본(本) 실험(實驗)은 배양토(培養土), 모래밭버섯버섯균(菌) 접종(接種) 및 질소시비(窒素施肥)가 1년생(年生) 해송분묘(海松盆苗)의 생장(生長)에 미치는 영향(影響)을 구명(究明)하기 위하여 실시(實施)하였다. 1. Vermiculite 구(區)에서는 사질양토구(砂質養土區)에서 보다 세근(細根) 및 균근수(菌根數)의 증가(增加)를 볼 수 있었고 묘고(苗高) 및 총건중(總乾重)이 증가(增加) 하였다. 2. 모래밭버섯균(菌) 접종구(接種區)가 무접종구(無接種區)보다 2년생(年生) 해송묘(海松苗)에 있어서 제일차(第一次) 측근수(側根數), 세근수(細根數) 및 총건중(總乾重)이 증가(增加)가 현저(顯著)하였다. 3. 2년생(年生) 해송분묘(海松盆苗) 생장(生長)은 전년도(前年度) 균근균(菌根菌) 접종(接種) 및 시비수준(施肥水準)에 크게 영향(影響)을 받았다.

• 한국균학회지
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• 제51권2호
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• pp.121-125
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• 2023

In this study, we examined the ectomycorrhiza (ECM) formed by a species of the genus Tuber, T. koreanum, with two indigenous Korean oak species, Quercus acutissima and Q. dentata. T. koreanum showed a high colonization rate for both oak species, with a higher mean colonization rate observed for Q. acutissima. The mycorrhizal characteristics of T. koreanum were described, revealing distinct morphological and anatomical differences between the ECM formed with the two host plants. This study is significant because it is the first to elucidate ECM formations of T. koreanum in indigenous oak trees. However, further studies are required to differentiate the ECM associations formed by T. koreanum from those of other Tuber species.

• Mycobiology
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• 제46권4호
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• pp.388-395
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• 2018

Radionuclides were deposited at forest areas in eastern parts of Japan following the Fukushima Daiichi Nuclear Power Plant incident in March 2011. Ectomycorrhizal (EM) fungi have important effects on radiocaesium dynamics in forest ecosystems. We examined the effect of colonization by the EM fungus Astraeus hygrometricus on the uptake of cesium (Cs) and potassium (K) by Pinus densiflora seedlings. Pine seedlings exhibited enhanced growth after the EM formation due to the colonization by A. hygrometricus. Additionally, the shoot Cs concentration increased after the EM formation when Cs was not added to the medium. This suggests that A. hygrometricus might be able to solubilize Cs fixed to soil particles. Moreover, the shoot K concentration increased significantly after the EM formation when Cs was added. However, there were no significant differences in the root K concentration between EM and non-EM seedlings. These results suggest that different mechanisms control the transfer of Cs and K from the root to the shoot of pine seedlings.