• Title/Summary/Keyword: must cell

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Revolution of Dead-Cell: Production of New Generation by Intracytoplasmic Dried-Sperm Injection in Mammal

  • Kim, Duk-Im;Kim, Chang Jin;Lee, Kyung-Bon
    • Reproductive and Developmental Biology
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    • v.39 no.3
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    • pp.69-76
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    • 2015
  • In a conventional sense, dried-spermatozoa are all dead and motionless due to the lost of their natural ability to penetrate oocytes both in vivo and in vitro. However, their nuclei are completely able to contribute to normal embryonic development even after long-term preservation in a dried state when the dried-spermatozoa are microinjected into the oocytes. In this sense, dried spermatozoa must still be alive. Thus, defining spermatozoa as alive or dead seems rather arbitrary. Several drying method of sperm including freeze-drying, evaporative/convective-drying and heat-drying were represented in this review. Although the drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genetic resources.

Complement regulation: physiology and disease relevance

  • Cho, Heeyeon
    • Clinical and Experimental Pediatrics
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    • v.58 no.7
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    • pp.239-244
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    • 2015
  • The complement system is part of the innate immune response and as such defends against invading pathogens, removes immune complexes and damaged self-cells, aids organ regeneration, confers neuroprotection, and engages with the adaptive immune response via T and B cells. Complement activation can either benefit or harm the host organism; thus, the complement system must maintain a balance between activation on foreign or modified self surfaces and inhibition on intact host cells. Complement regulators are essential for maintaining this balance and are classified as soluble regulators, such as factor H, and membrane-bound regulators. Defective complement regulators can damage the host cell and result in the accumulation of immunological debris. Moreover, defective regulators are associated with several autoimmune diseases such as atypical hemolytic uremic syndrome, dense deposit disease, age-related macular degeneration, and systemic lupus erythematosus. Therefore, understanding the molecular mechanisms by which the complement system is regulated is important for the development of novel therapies for complement-associated diseases.

Optimization of the Condition of Immobilized Photobacterium phosphoreum with Strontium Alginate (Strontium Alginate를 담체로 한 Photobacterium phosphoreum 고정화 조건의 최적화)

  • 이홍주;김현숙;정계훈;이은수;전억한
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.136-144
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    • 1999
  • Since the condition of immobilization must be optimized, it is very important to know whether and on how conditions bacterial cells retain their metabolic activity during immobilization process. A bioluminescence intensity had the maximum value when cell concentrations were between 1.0 and 1.2 measured at O.D660. The strontium alginate was used as an immobilization matrix and two independent factors for immobilization of Photobacterium phosphoreum with strontium alginate were optimized with the response surface methodology(RSM) considering degree of bioluminescence. As a result, the optimum concentration for immobilization was found to be 2.4%(w/w) for sodium alginate and 0.31M for strontium chloride, respectively. A dilution was carried out with 2.5%(w/v) NaCl solution that is an optimum environmental condition for growth of P. phosphoreum. Under the such condition of immobilization, hardness could be predicted as 4.66$\times$104N/$m^2$ and it took different time according to the volume of matrix to be immobilized completely.

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Measurement and Design Review of Contact Force by Garter Spring in Connection between Circuit Breaker and Bus Bar (차단기와 모선과의 연결시 가터스프링에 의한 접촉력의 측정 및 설계 고찰)

  • 조상순;안길영;박우진;오일성
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.10a
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    • pp.942-945
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    • 2002
  • A garter spring, which is a long. special, close-coiled extension spring with its ends joined to form a ring, is used in connection between vacuum circuit breaker and bus bar in switchgear. To carry short-time current and resist welding at the contact surface in the connection, the garter spring must transmits an uniform contact force to the contact surface through the contact chips arranged in the circumference of bus bar. In this paper, the system for measurement of the contact force by the garter spring is developed. Using the developed measurement system, the design of the connection structure including the garter spring is reviewed to obtain the uniform contact forces in all of contact chips.

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Microalgal Culture Conditions for Utilization of Flue Gas from Rice Husk Incinerator (왕겨 소각로 배연가스 이용을 위한 미세죠류 배양 조건 확립)

  • 박승제;조성호;이진석;정용섭
    • Journal of Biosystems Engineering
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    • v.24 no.1
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    • pp.9-18
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    • 1999
  • This study was performed to investigate the optimum microalgal culture conditions using flask culture and to find the feasibility of using the flue gas of the rice husk incinerator for cultivating the microalgae. The optimum initial pH of media was 4.5 for the microalgae culture, and the intermittently illuminated culture was more effective than the continuous illuminated culture. Thus, the balance between photosynthesis and formative metabolism must be considered thoroughly to cultivate microalgal cells. The optimum CO2 concentrations were in the range of 7 to 10%, and the optimum temperature was about 35$^{\circ}C$ in both the daytime and the nighttime for the culture. When flue gas of the rice husk incinerator was applied to the microalgae culture using stirred photobioreactor, the dry cell weight was 0.026 g dry biomass/hr$.$l. The results obtained in experiments indicated that the flue gas was effective for microalgae culture without any limitations.

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Temperature-Adaptive Back-Bias Voltage Generator for an RCAT Pseudo SRAM

  • Son, Jong-Pil;Byun, Hyun-Geun;Jun, Young-Hyun;Kim, Ki-Nam;Kim, Soo-Won
    • ETRI Journal
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    • v.32 no.3
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    • pp.406-413
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    • 2010
  • In order to guarantee the proper operation of a recessed channel array transistor (RCAT) pseudo SRAM, the back-bias voltage must be changed in response to changes in temperature. Due to cell drivability and leakage current, the obtainable back-bias range also changes with temperature. This paper presents a pseudo SRAM for mobile applications with an adaptive back-bias voltage generator with a negative temperature dependency (NTD) using an NTD VBB detector. The proposed scheme is implemented using the Samsung 100 nm RCAT pseudo SRAM process technology. Experimental results show that the proposed VBB generator has a negative temperature dependency of -0.85 $mV/^{\circ}C$, and its static current consumption is found to be only 0.83 ${\mu}A$@2.0 V.

Regulation of Intestinal Immune System by Dendritic Cells

  • Ko, Hyun-Jeong;Chang, Sun-Young
    • IMMUNE NETWORK
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    • v.15 no.1
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    • pp.1-8
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    • 2015
  • Innate immune cells survey antigenic materials beneath our body surfaces and provide a front-line response to internal and external danger signals. Dendritic cells (DCs), a subset of innate immune cells, are critical sentinels that perform multiple roles in immune responses, from acting as principal modulators to priming an adaptive immune response through antigen-specific signaling. In the gut, DCs meet exogenous, non-harmful food antigens as well as vast commensal microbes under steady-state conditions. In other instances, they must combat pathogenic microbes to prevent infections. In this review, we focus on the function of intestinal DCs in maintaining intestinal immune homeostasis. Specifically, we describe how intestinal DCs affect IgA production from B cells and influence the generation of unique subsets of T cell.

Phenolic Compounds in Plant Foods: Chemistry and Health Benefits

  • Naczk, Marian;Shahidi, Fereidoon
    • Preventive Nutrition and Food Science
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    • v.8 no.2
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    • pp.200-218
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    • 2003
  • Phenolic compounds in food and plant materials belong to the simple phenols, phenolic acids, coumarins, flavonoids, stilbenes, tannins, lignans and lignins, all of which are considered as secondary plant metabolites. These compounds may be synthesized by plants during normal development or in response to stress conditions. Phenolics are not distributed uniformly in plants. Insoluble phenolics are components of cell walls while soluble ones are present in vacuoles. A cursory account of phenolics of cereals, beans, pulses, fruits, vegetables and oilseeds is provided in this overview. The information on the bioavailability and absorption of plant phenolics remains fragmentary and diverse. Pharmacological potentials of food phenolics ave extensively evaluated. However, there are many challenges that must be overcome in order to fully understand both the function of phenolics in plant as well as their health effects.

A study on Optical Characteristics of Charged Toner Particle Type Display (토너입자형 디스플레이의 광학특성에 관한 연구)

  • Kim, Baek-Hyun;Park, Sun-Woo;Kim, Young-Cho
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.22 no.1
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    • pp.86-92
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    • 2009
  • We fabricated the charged particle type display using opposite-charged two toner particles. The particles were composed of polymer, colorant($TiO_2$, carbon black) and external additives(nano-sized silica and so on). In fabrication process we controlled the putting layers of this toner particles on the inner panel. To get the effect of number of layers ($1{\sim}3$), we measured the threshold, driving and breakdown voltages, reflectivity, viewing angle, and color characteristics using RT-300 anisotropic scattering system. We ascertained that these voltages are increased with increasing of layers of particles. Cell gap between electrodes must be enlarged with increment of layers and the size of particles. The lumping phenomena of particles at near of the rib observed by optical system with same CIE values.

Optimization of Chiral Dopant and Rubbing Direction in Liquid Crystal Display

  • Lee, Kyung-Jun;Park, Jun-Baek;Park, Jong-Min;Kim, Hoe-Chang;Seo, Jae-Ho;Kim, Yoo-Jin;Jung, Byung-Hwan
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08a
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    • pp.948-951
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    • 2007
  • In order to improve the performance of LCD, chiral dopant is added to liquid crystal mixture. When we decide the rubbing direction, we must consider the rotation direction of liquid crystal molecules by chiral dopant. When the rotation direction of liquid crystal molecules caused by dielectric torque decided by rubbing direction and that decided by chiral dopant are coincided, the performance of LCD would be improved along to our initial cell design intentions.

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