• Structural Engineering and Mechanics
• /
• v.14 no.2
• /
• pp.237-243
• /
• 2002

It is well known that torsionally unbalanced buildings are more vulnerable to earthquake hazards than are the regular structural systems. In this paper, a parametric investigation is presented, in order to observe the amplification in the internal forces, when increased eccentricities are used instead of the ones corresponding to the 5% accidental eccentricity. A series of five, ten-story framed and walled structures, with rather high torsional irregularity coefficients, are selected and a numerical test procedure is applied. Numerical results show that the maximum amplification in the internal forces at the most critical beams and columns at the flexible sides of the structures is about 10%. It is concluded that, more serious measures in the codes are needed in the case of this rather dangerous type of irregularity.

• Structural Engineering and Mechanics
• /
• v.23 no.2
• /
• pp.177-193
• /
• 2006

Determining the hysteretic energy demand and dissipation capacity and level of damage of the structure to a predefined earthquake ground motion is a highly non-linear problem and is one of the questions involved in predicting the structure's response for low-performance levels (life safe, near collapse, collapse) in performance-based earthquake resistant design. Neural Network (NN) analysis offers an alternative approach for investigation of non-linear relationships in engineering problems. The results of NN yield a more realistic and accurate prediction. A NN model can help the engineer to predict the seismic performance of the structure and to design the structural elements, even when there is not adequate information at the early stages of the design process. The principal aim of this study is to develop and test multi-layered feedforward NNs trained with the back-propagation algorithm to model the non-linear relationship between the structural and ground motion parameters and the hysteretic energy demand in steel moment resisting frames. The approach adapted in this study was shown to be capable of providing accurate estimates of hysteretic energy demand by using the six design parameters.

• Earthquakes and Structures
• /
• v.8 no.1
• /
• pp.205-224
• /
• 2015

A new method for designing moment resisting concrete frames failing in a global mode is presented in this paper. Starting from the analysis of the typical collapse mechanisms of frames subjected to horizontal forces, the method is based on the application of the kinematic theorem of plastic collapse. The beam section properties are assumed to be known quantities, because they are designed to resist vertical loads. As a consequence, the unknowns of the design problem are the column sections. They are determined by means of design conditions expressing that the kinematically admissible multiplier of the horizontal forces corresponding to the global mechanism has to be the smallest among all kinematically admissible multipliers. In addition, the proposed design method includes the influence of second-order effects. In particular, second-order effects can play an important role in the seismic design and can be accounted for by means of the mechanism equilibrium curves of the analysed collapse mechanism. The practical application of the proposed methodology is herein presented with reference to the design of a multi-storey frame whose pattern of yielding is validated by means of push-over analysis.

• Journal of Korean Neurosurgical Society
• /
• v.62 no.3
• /
• pp.336-343
• /
• 2019

Epilepsy surgery revealed dramatically improved seizure outcomes over medical therapy in drug-resistant epilepsy patients. Children with epilepsy, however, have multiple epileptic focuses which require multilobar resection for better seizure outcome. Multilobar resection has not only the several severe surgical complications, such as hydrocephalus and shunt-related craniosynostosis, due to intracranial volume reduction. Isolation method (disconnection surgery) was progressively studied over epileptic focus removal (resective surgery) for seizure control. This concept was first introduced for functional hemispherotomy, and its primary principle is to preserve the vital vascularized brain that is functionally disconnected from the contralateral healthy brain. Currently in most epilepsy centers, the predominant disconnection surgical methods, including functional hemispherotomy, are continually being refined and are showing excellent results. They allow the functional isolation of the hemisphere or multi-lobe, affected by severe epilepsy. This review describes recent findings concerning the indication, surgical technique, seizure outcome and complications in several disconnection surgeries including the functional hemispherotomy for refractory pediatric epilepsy.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2001.11a
• /
• pp.110-110
• /
• 2001

Histone deacetylase(HDAC), a neuclear enzyme that regulates gene trascription and the assembly of newly synthesized chromatin, has received much attention in recent literature. The explosion of activity in this field has yielded the cloning of a mammalian gene which encodes a complementary histone acetyl trasferases. Several cyclic tetrapeptide inhibitors of HDAC has been reported to affect the hyperacetylation of mammalian and plant histones. Apicidin, a natural product HDAC inhibitor recently isolated at Merck Research Laboratories, induces therapeutic applications as a broad spectrum antiprotozoal agent to multi-drug resistant malaria and a potential antitumor agnet. The biological activity of apicidin appears to be attributable to inhibition of apicocomplexan HDAC at low nanomolar concentrations.

• BMB Reports
• /
• v.51 no.9
• /
• pp.450-455
• /
• 2018

Tamoxifen (TAM) is commonly used to treat estrogen receptor (ER)-positive breast cancer. Despite the remarkable benefits, resistance to TAM presents a serious therapeutic challenge. Since several HOX transcription factors have been proposed as strong candidates in the development of resistance to TAM therapy in breast cancer, we generated an in vitro model of acquired TAM resistance using ER-positive MCF7 breast cancer cells (MCF7-TAMR), and analyzed the expression pattern and epigenetic states of HOX genes. HOXB cluster genes were uniquely up-regulated in MCF7-TAMR cells. Survival analysis of in slico data showed the correlation of high expression of HOXB genes with poor response to TAM in ER-positive breast cancer patients treated with TAM. Gain- and loss-of-function experiments showed that the overexpression of multi HOXB genes in MCF7 renders cancer cells more resistant to TAM, whereas the knockdown restores TAM sensitivity. Furthermore, activation of HOXB genes in MCF7-TAMR was associated with histone modifications, particularly the gain of H3K9ac. These findings imply that the activation of HOXB genes mediate the development of TAM resistance, and represent a target for development of new strategies to prevent or reverse TAM resistance.

• Korean Journal of Veterinary Service
• /
• v.28 no.1
• /
• pp.71-79
• /
• 2005

In this study, a total of 2,119 samples was taken from bovine feces and carcass from March 2002 to December 2003. And those were examined for the presence of enterohemorrhagic E coli O157:H7. The properties of the isolates were characterized for biochemical features, serotypes, virulence genes and antimicrobial susceptibility. Forty five strains($3.7\%$) of E coli O157:H7 were isolated from 1,208 fecal samples and were not detected in carcass using immunomagnetic separation technique and selective media. In multiplex PCR using stx1, stx2, eaeA and hlyA primers, the amplified bands at 180 bp, 255bp, 384bp and 534bp were observed, respectively. In antimicrobial susceptibility test, all isolates were susceptible to amoxicillin/clavulanic acid and cefazolin. The isolates were most resistant to sulfisoxazole($24.4\%$), followed by streptomycin($22.2\%$), tetracycline($20.0\%$). Eight strains($17.8\%$) of 45 isolates showed the multi-resistant patterns with over 3 drugs.

• Journal of Wetlands Research
• /
• v.13 no.3
• /
• pp.697-705
• /
• 2011

The aim of this study was to examine ozonation disinfection efficiency for Escherichia coli DH5alpha removal, containing the multi-resistance plasmid pB10 as well as chlorination disinfection efficiency. In addition, plasmid pB10 removal rates were estimated by ozonation and chlorination. The removal efficiency of pB10 via ozonation was about 2 to 4 times higher than chlorination. High removal efficiency of pB10 is likely due to OH radical produced during ozonation. These results suggest that integration of advanced oxidation process such as ozonation (or photocatalytic oxidation) with conventional disinfection such as chlorination may be needed for effective control of antibiotic resistant bacteria and genetic materials.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.7
• /
• pp.974-981
• /
• 2020

Sequence type 410 (ST410) of Escherichia coli is an extraintestinal pathogen associated with multi drug resistance. In this study, we aimed to investigate the horizontal propagation pathway of a high-risk clone of E. coli ST410 that produces Klebsiella pneumoniae carbapenemase (KPC). blaKPC-encoding E. coli and K. pneumoniae isolates were evaluated, and complete sequencing and comparative analysis of blaKPC-encoding plasmids from E. coli and K. pneumoniae, antimicrobial susceptibility tests, polymerase chain reaction, multilocus sequence typing, and conjugal transfer of plasmids were performed. Whole-genome sequencing was performed for plasmids mediating KPC-2 production in E. coli and K. pneumoniae clinical isolates. Strains E. coli CPEc171209 and K. pneumoniae CPKp171210 were identified as ST410 and ST307, respectively. CPEc171209 harbored five plasmids belonging to serotype O8:H21, which is in the antimicrobial-resistant clade C4/H24. The CPKp171210 isolate harbored three plasmids. Both strains harbored various additional antimicrobial resistance genes. The IncX3 plasmid pECBHS_9_5 harbored blaKPC-2 within a truncated Tn4401a transposon, which also contains blaSHV-182 with duplicated conjugative elements. This plasmid displayed 100% identity with the IncX3 plasmid pKPBHS_10_3 from the K. pneumoniae CPKp171210 ST307 strain. The genes responsible for the conjugal transfer of the IncX3 plasmid included tra/trb clusters and pil genes coding the type IV pilus. ST410 can be transmitted between patients, posing an elevated risk in clinical settings. The emergence of a KPC-producing E. coli strain (ST410) is concerning because the blaKPC-2-bearing plasmids may carry treatment resistance across species barriers. Transgenic translocation occurs among carbapenem-resistant bacteria, which may spread rapidly via horizontal migration.

• Journal of the Korean Society of Safety
• /
• v.35 no.4
• /
• pp.32-39
• /
• 2020

The fire compartments with fire-resistant construction are installed in the principal structural parts of a building in order to reduce damage in the event of a building fire. As a fire may spread through a crack in the fire compartment, the firestop with secured performance is used according to the procedure, methods, and standards specified in the detailed operation guideline. According to the current detailed operation guideline, vertical members (wall penetration) and horizontal members (floor penetration) are classified into different categories respective to each other for the classification of the firestop. Therefore, an accreditation applicant must apply for the performance test for each structure even if the wall and the floor have the same structure. Also, Grade T is used for the firestop that penetrates the fire compartment. However, in the case of foreign countries, the use of Grade F for the firestop is allowed even if it penetrates the fire compartment. The result of the precedent studies also showed that there was a significantly low possibility of fire to spread even if Grade F was applied for a metallic duct that penetrated the fire compartment. In this study, the improved scheme for the classification and performance standard of firestops was presented by analyzing the results of precedent studies regarding the firestop and domestic and overseas firestop qualification systems.