Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

The isolation and antimicrobiol susceptibility of Escherichia coli O157:H7 on bovine feces and carcass

소 분변과 도체에서 E coli O157:H7의 분리와 항생제 감수성

  • Chae Hee-Sun (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Jong-Hwa (Seoul Metropolitan Health & Environment Research Institute) ;
  • Kim Gyu-Hyeon (Seoul Metropolitan Health & Environment Research Institute) ;
  • Choi Tae-Seok (Seoul Metropolitan Health & Environment Research Institute) ;
  • Shin Bang-Woo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee Duck-Joo (Seoul Metropolitan Health & Environment Research Institute) ;
  • Lee Jung-Hark (Seoul Metropolitan Health & Environment Research Institute)
  • 채희선 (서울특별시 보건환경연구원) ;
  • 김종화 (서울특별시 보건환경연구원) ;
  • 김규현 (서울특별시 보건환경연구원) ;
  • 최태석 (서울특별시 보건환경연구원) ;
  • 신방우 (서울특별시 보건환경연구원) ;
  • 이덕주 (서울특별시 보건환경연구원) ;
  • 이정학 (서울특별시 보건환경연구원)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, a total of 2,119 samples was taken from bovine feces and carcass from March 2002 to December 2003. And those were examined for the presence of enterohemorrhagic E coli O157:H7. The properties of the isolates were characterized for biochemical features, serotypes, virulence genes and antimicrobial susceptibility. Forty five strains($3.7\%$) of E coli O157:H7 were isolated from 1,208 fecal samples and were not detected in carcass using immunomagnetic separation technique and selective media. In multiplex PCR using stx1, stx2, eaeA and hlyA primers, the amplified bands at 180 bp, 255bp, 384bp and 534bp were observed, respectively. In antimicrobial susceptibility test, all isolates were susceptible to amoxicillin/clavulanic acid and cefazolin. The isolates were most resistant to sulfisoxazole($24.4\%$), followed by streptomycin($22.2\%$), tetracycline($20.0\%$). Eight strains($17.8\%$) of 45 isolates showed the multi-resistant patterns with over 3 drugs.

Keywords

References

  1. Ostroff SM, Tarr PI, Neill MA, et aI. 1989. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J Infect Dis 160 : 994-998 https://doi.org/10.1093/infdis/160.6.994
  2. Karmali MA, Steele BT, Petrie M, et aI. 1983. Sporadic cases of haemolytic uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 1 : 619-620
  3. Iwasa M, Makino S, Asakura H et al. 1999. Detection of Escherichia coli O157:H7 from musca domestica (Diptera: Muscidae) at a cattle farm in Japan. J Med Entomol 36 : 108-112 https://doi.org/10.1093/jmedent/36.1.108
  4. 국립보건원. 1994. 감염병 발생정보 5: 131-142
  5. Louie M, Read S, Louie L, et al. 1999. Molecular typing methods to investigate transmission of Escherichia coli O157:H7 from cattle to humans. Epiderniol Infect 123 : 17-24 https://doi.org/10.1017/S0950268899002551
  6. Brees P, Morgan IM, Ackermann TW, et al. 2000. Cattle lack vascular receptors for Escherichia coli O157:H7 shiga toxins. Pans 97 : 10325-10329 https://doi.org/10.1073/pnas.190329997
  7. Thomas A, Cheasty T, Frost JA, et al. 1!m. Verocytotoxin -producing Escherichia coli, particularly serogroup O157, associated with human infections in England and Wales : 1992-4. Epiderniol Infect 117 : 1-10
  8. 강동현. 1997. 식품 병원균인 Escherichia coli O157:H7의 중요성. 한국식품위생안전성학회지 12 : 367-378
  9. Hideki, Jun K, Muneo S, et al. 2001. Prevalence and characteristics of shiga toxin-producing Escherichia coli from healthy cattle in Japan. Appl Environ Microbiol 67 : 484-489 https://doi.org/10.1128/AEM.67.1.484-489.2001
  10. 정석찬, 정병열, 윤장원 등. 1998. 쇠고기 중 Escherichia coli O157:H7 신속검출을 위한 multiplex-PCR 기법 개발. 대한수의학회지 38 : 173-181
  11. Feng P, Monday SR. 2000. Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes, Mol Cell Probes 14 : 333-337 https://doi.org/10.1006/mcpr.2000.0323
  12. Johnsen G, Wasteson Y, Heir E, et al. 2001. Escherichia coli O157:H7 in feces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999. Int J Food Microbiol 65 : 193-200 https://doi.org/10.1016/S0168-1605(00)00518-3
  13. Wang G, Zhao T, Doyle MP 1996. Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces. Appl Environ Microbiol 62 : '2567-2570
  14. Mead PS, Griffin PM. 1998. Escherichia coli O157:H7. Lancet 352 : 1207-1212 https://doi.org/10.1016/S0140-6736(98)01267-7
  15. Adwan K, Abu-Hasan N, Essawi T. 2002. Isolation and characterisation of shiga toxigenic Escherichia coli strains from northern Palestine. J Med Microbiol 51 : 332-33 https://doi.org/10.1099/0022-1317-51-4-332
  16. Hu Y, Zhang Q, Meitzler JC. 1999. Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by multiplex PCR. J Appl. Microbiol 87 : 867-876 https://doi.org/10.1046/j.1365-2672.1999.00938.x
  17. Paton A W, Paton JC. 1998. Detection and characterization of shiga toxigenic Escherichia coli by using multiplex PCR assays for stxl, stx2, eaeA, entero-hemorrhagic E. coli hlyA, $rfbo_{111}$, and $rfbo_{157}$. J Clin Microbiol 36 : 598-602
  18. National Committee for Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests-6th ed. NCCLS, Wayne, Pa
  19. Bonardi S, Maggi E, Bottarelli A, et al. 1999. Isolation of vero cytotoxinproducing Escherichia coli O157:H7 from cattle at slaughter in Italy. Vet Microbiol 67 : 203-211 https://doi.org/10.1016/S0378-1135(99)00039-5
  20. Heuvelink AE, Biggelar FL, Boer, E, et al. 1998. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 strains from dutch cattle and sheep. J Clin Microbiol 36 : 878-882
  21. Lahti E, Keskimaki M, Rantala L, et al. 2001. Occurrence of Escherichia coli O157 in finnish cattle. Vet Microbiol 79 : 239-254 https://doi.org/10.1016/S0378-1135(00)00355-2
  22. 차인호, 김용환. 1996. 동물분변에서 Escherichia coli O157:H7의 분리 및 이들 균이 생산하는 Verotoxin-2의 생물화학적 특성(1. 소와 대지의 분변에서 Escherichia coli O157:H7의 분리 및 Verotoxin-2 생산에 관여하는 파아지의 분리에 관하여). 대한수의학회지 36 : 371-378
  23. 고주언, 홍종해. 1997. 도체표면의 분변오염과 verotoxin 생성. 한국식품위생안전성학회지 12 : 78-82
  24. Leclercq A, Lambert B, Pierarad D, et al. 2001. Particular biochemical profiles for enterohemorrhagic Escherichia coli O157: H7 isolates on the ID 32E system. J Clin Microbiol 39 : 1161-1164 https://doi.org/10.1128/JCM.39.3.1161-1164.2001
  25. Meng J. Zhao S, Doyle MP, et al. 1998. Antibiotic resistance of Escherichia coli O157:H7 and O157:NM isolated from animals, food and humans. J Food Prot 61 : 1511-1514 https://doi.org/10.4315/0362-028X-61.11.1511
  26. Schroeder CM, Zhao C, Debroy C, et al. 2002. Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine and food. Appl Environ Microbiol 68 : 576-581 https://doi.org/10.1128/AEM.68.2.576-581.2002
  27. Threlfall EJ, Ward LR, Frost JA, et al. 2000. The emergene and spread of antibiotic resistance in food-borne bacteria. Int J Food Microbiol 62 : 1-5 https://doi.org/10.1016/S0168-1605(00)00351-2
  28. Vila J, Vargas M, Casals C, et al. 1999. Antimicrobial resistance of diarrheagenic Escherichia coli isolated from children under the age of 5 years from Ifakara, Tanzania. Antimicrob Agents Chemother 43 : 3022-3024
  29. Dupont H, Timsit JF, Souweine B, et al. 1996. Antimicrobial susceptibility of Escherichia coli O157 and other entero haemorrhagic Escherichia coli isolated in Italy. Clin Reanim Malad Infect 15 : 351-353