• Korean Journal of Pharmacognosy
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• v.13 no.4
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• pp.145-152
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• 1982

Three species of genus Polygonum, namely P. cuspidatum, P. sachalinense and P. ellipticum are distributed in Korea. Polygonum ellipticum Migo is a perennial herb in Polygonaceous plants. The root of the plant (Polygoni Rhizoma, 'Ho-jang') have been used as laxative, diuretic and for the treatment suppurative dermatitis in the oriental medicine. As the part of the study for the comparison of the three species in their components, the authors attempted to isolate the anthraquinones and stilbene derivative from the rhizome of P. ellipticum. The methanolic extract of dried rhizome of this plant was fractionated into ether soluble and insoluble fraction and each fraction was applied to column chromatography to isolate above mentioned components. Anthraquinone derivatives were isolated first; comp. I, mp $204{\sim}205^{\circ}$ (physcion), comp. II, mp $254{\sim}255^{\circ}$ (emodin), comp. IV, mp $191{\sim}192^{\circ}$ $(emodin-8-O-{\beta}-D-glucoside)$ and comp. III, mp $280{\sim}282^{\circ}$ $({\beta}-sitosterol-glucoside)$. They were identified by chemical properties and UV, IR and NMR spectra and by the direct comparison with authentic samples. Stilbene derivative was isolated secondly; comp. V, mp $255{\sim}256^{\circ}$ which was reported to possess antibacterial and antifungal activities.

• Journal of Korea Society of Industrial Information Systems
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• v.8 no.4
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• pp.26-30
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• 2003

The Lanczos algorithm is the most commonly used in approximating a small number of extreme eigenvalues for symmetric large sparse matrices. Global communications in MP(Message Passing) parallel computer decrease the computation speed. In this paper, we introduce the s-step Lanczos method, and s-step method generates reduction matrices which are similar to reduction matrices generated by the standard Lanczos method. One iteration of the s-step Lanczos algorithm corresponds to s iterations of the standard Lanczos algorithm. The s-step method has the minimized global communication and has the superior parallel properties to the standard method. These algorithms are implemented on Cray T3E and performance results are presented.

• Journal of Microbiology
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• v.42 no.2
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• pp.87-93
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• 2004

Twelve mecoprop-degrading bacteria were isolated from soil samples, and their genetic and phenotypic characteristics were investigated. Analysis of 16S rDNA sequences indicated that the isolates were related to members of the genus Sphingomonas. Ten different chromosomal DNA patterns were obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences from the 12 isolates. The isolates were found to be able to utilize the chiral herbicide meco-prop as a sole source of carbon and energy. While seven of the isolates were able to degrade both (R)-and (S)-mecoprop, four isolates exhibited enantioselective degradation of the (S)-type and one isolate could degrade only the (R)-enantiomer. All of the isolates were observed to possess plasmid DNAs. When certain plasmids were removed from isolates MPll, MP15, and MP23, those strains could no longer degrade mecoprop. This compelling result suggests that plasmid DNAs, in this case, conferred the ability to degrade the herbicide. The isolates MP13, MP15, and MP24 were identified as the same strain; however, they exhibited different plasmid profiles. This indicates that these isolates acquired dif-ferent mecoprop-degradative plasmids in different soils through natural gene transfer.

• Culinary science and hospitality research
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• v.12 no.4 s.31
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• pp.131-139
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• 2006

This study carried out the standardization of processing conditions in mustard powder (MP) for quality improvement and suggested a recycling scheme of mustard oil(MO). Pungent taste in MP and MO was estimated using allylisothiocyanate (AITC) content as a marker. Recovery of crude oil from mustard seed (MS) was best by the cold pressing method. Residual AITC content at $30^{\circ}C$ pressing was 0.54% and 0.42% at $230^{\circ}C$. But residual AITC contents in MOs were 92ppm, 139ppm, respectively. The residual AITC content in MP was the highest (0.54%) when the moisture content in MP was 4.5%. The residual content of volatile oil in MP and MO showed similar results. In summary, crude oil must be removed from MS using the cold pressing method.

• Bulletin of the Korean Chemical Society
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• v.10 no.4
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• pp.377-381
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• 1989

The spectroscopic properties of $FO^+$ and FO were investigated by ab initio calculation. Several different levels of theory, $MP3/6-31G^*,\;MP4/6-311G^*\;and\;CISD/6-31G^*$, were tried and compared with experimental results of FO. In the overall performance the CISD showed the best agreement. Based on these results the spectroscopic constants of $FO^+$ are predicted.

• Clinical and Experimental Pediatrics
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• v.53 no.2
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• pp.178-183
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• 2010

Purpose : The aim of this study was to identify mutations associated with macrolide resistance in Mycoplasma pneumoniae (MP) and to establish a cultural method to determine antimicrobial susceptibility. Methods : Nasopharyngeal aspirates (NPAs) were collected from 62 children diagnosed with MP pneumonia by a serologic method or polymerase chain reaction. The 23S rRNA and L4 ribosomal protein genes of MP were amplified and sequenced. To identify mutations in these 2 genes, their nucleotide sequences were compared to those of the reference strain M129. MP cultivation was carried out for 32 (28 frozen and 5 refrigerated) NPAs and M129 strain using Chanock's glucose broth and agar plate in a 5% $CO_2$ incubator at $37^{\circ}C$ and examined at 2-3 day intervals for 6 weeks. Results : Among the 62 specimens, 17 had M144V mutations in ribosomal protein L4. The A2064G mutation was observed in 1 specimen; its 23S rRNA gene was successfully sequenced. Culture for MP was successful from the M129 strain and 2 of the 5 NPAs that were refrigerated for no longer than 3 days. However, MP did not grow from the 28 NPAs that were kept frozen at $-80^{\circ}C$ since 2003. Conclusion : We found the M144V mutation of L4 protein to be common and that of domain V of 23S rRNA gene was relatively rare among MP. Studies on the prevalence of macrolide-resistant MP and the relationship between the mutations of 23S rRNA gene and ribosomal protein L4 will aid in understanding the mechanism of macrolide resistance in MP.

• The Korean Journal of Nuclear Medicine Technology
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• v.19 no.1
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• pp.72-80
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• 2015

Purpose Serum estradiol ($E_2$) measurement is requested for diagnosing menstrual cycles, ovulation induction, infertility, and menopause. $E_2$ is measured using several methods and kits including radioimmunoassay (RIA) and chemiluminescece immunoassay (CLIA). The purpose of this study was to evaluate quality of these methods and to compare them with each other. Materials and Methods Seven radioimmunoassay kits and two CLIA methods were included in the analysis. Using standard samples and patient samples, intra-assay precision, inter-assay precision, correlation between other methods, sensitivity, and recovery rate were evaluated. Results For all tested kits and methods, coefficients of variance (CVs) of intra-assay precision test were 10.9~13.6% in low-level samples and less than 10% in medium and high-level samples. CVs of inter-assay precision test were 10.8~12.3% in low-level samples and less than 10% in medium and high-level samples with all tested kits and methods. Recovery rates were $92.7{\pm}12.4%$ for SIEMENS, $101.4{\pm}18.4%$ for DIAsource, $95.1{\pm}11.5%$ for AMP, $108.4{\pm}18.5%$ for BECKMAN COULTER, $104.2{\pm}13.5%$ for BECKMAN COULTER Ultra Sensitive, $101.3{\pm}11.6%$ for CIS Bio, and $93.1{\pm}13.2%$ for MP kits. Sensitivity was 7.5, 6.2, 5.7, 6.2, 5.3, 4.5, and 5.5 pg/mL for SIEMENS, DIAsource, AMP, BECKMAN COULTER, BECKMAN COULTER Ultra Sensitive, CIS Bio, and MP kits, respectively. The measurement by MP kit was slightly higher than those by other kits in low-level samples, and the measurement by E170 was slightly higher than those of other kits in medium and high-level samples. In the measurement of standard sample for external quality control, SIEMENS kit produced relatively lower values whereas E170, Architect, and MP kits produced relatively higher values compared with other kits. Conclusion All tested kits for $E_2$ measurement have satisfactory performance for clinical use. However, correlation between kits should be considered when test kits are to be changed, because some pairs of kits do not have correlations with each other.

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.6
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• pp.1071-1083
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• 1996

This study was carried out to obtain some fundamental data for designing thermally comfortable Korean folk clothes (KFC) which were classified into 4 groups according to seasons and materials. It was conducted to find the relationship among structural, thermal, and moisture transfer properties, and then the properties of heat and mositure transfer while changing thickness of air layer of selected similar fabrics with various thickness were examined. The summarized results of this study were as follows ; 1. The relationship between the structrual and thermal properties showed high positive correlation with thickness SE thermal insulation value (TIV), porosity & TIV. 2. The relationship between the structural and mositure transfer properties showed high negative correlation with thickness & moisture vapor permeability (Mp), porosity & Mp respectively. 3. The relationship between the heat and moisture transfer properites showed high negative correlation with qmax, air permeability (Ap), Mp & TIV while showed high positive with Ap & Mp respectively. 4. Resistance to dry heat transfer according to thickness of air layer for KFC was increassed rapidly up to 2 mm and above this increasing rate was reduced. Resistance to evaporative heat transfer was remarkable changes in 6∼8 mm but slowly in 6∼8 man. 5. Mioisture permeability index (i) according to thickness of air layer for KFC showed the greatest value at 2 mm but had not difference between groups according to thickness.

• Industrial Engineering and Management Systems
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• v.14 no.4
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• pp.372-378
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• 2015

In labor-intensive enterprise, such as garment factory, assembly line is widely used as a manufacturing process for reducing costs and production time. However, for the sake of the various working capacity of worker, idle or delay may happen and influence the rear processes. If these unforeseeable delay happened continuously, it may influence the whole manufacturing process and a model, which is called limited-cycle model with multiple periods (LCMwMP), is assumed to evaluate the influence risk. In order to minimize the risk, the assignment of the workers is focused on. In this paper, we deal with an assembly line as LCMwMP model when two kinds of workers exist, whose efficiency is assumed to two different groups. We consider an optimization problem for finding an assignment of workers to the line that minimizes total expected risk, which is exchanged to expected cost by reset model of LCMwMP. First, reset model as a simple model of LCMwMP is introduced. Then, some hypotheses of the rules of the optimal worker assignment are proposed and some numerical experiments are researched assuming the processing time as Erlang distribution. Finally, the other rules on other certain conditions are discussed.

• The Plant Pathology Journal
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• v.32 no.5
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• pp.452-459
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• 2016

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-${\beta}$-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved with 1 mM IPTG for 4 h. Identity of the expressed protein was confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting. The expressed 41 kDa protein was purified under denaturing condition by affinity chromatography, reconfirmed by Western blotting and plate-trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA) before being used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-GFLV MP immunoglobulines (IgGs) and conjugated IgGs detected the expressed MP and GFLV virions in infected grapevines when used in PTA-ELISA, double antibody sandwich-ELISA, and Western blotting. This is the first report on the production of anti-GFLV MP polyclonal antibodies and application for the virus detection.