• Journal of Microbiology
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• v.40 no.3
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• pp.241-244
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• 2002

Fungi commonly encountered on monocotyledonous substrates were evaluated for their in vitro ability to produce enzymes involved in lignocellulose breakdown. Most were capable of structural polysac-charide utilization, but few produced enzymes associated with lignin breakdown. None of the mono-cotyledon-inhabiting fungi produced reactions as strongly as wood decay fungi.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.53-61
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• 1994

This study was carried out in Mt. Backdu which is located at the borderland between Korea and China, to offer the information and help the people who in order to study and utilize the northernresource plants in future. Actually, the exploration and investigation were executed only in china sideof Mt. Backdu and its outskirts.Totally, 100 families, 281 genus and 703 species were investigated. They were classified into 26 spe-cies of pteridophyta, 14 species of gymnospermae, 101 species of monocotyledons and 562 species of di-cotyledons by general taxonomic classification, and 594 medicinal plants(84.5% ), 296 ornamentalplants(38.3% ), 161 edible plants(22.9% ), 92 industrial plants, 67 honey plants and 17 forage plantsby use.

• Journal of Forest and Environmental Science
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• v.28 no.1
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• pp.8-11
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• 2012

The Kashmir Himalaya, known for its indigenous and endemic flora, also provides home to a large number of exotic plants, which exhibit a wide taxonomical and distributional stretch. Although, some preliminary studies have been carried out in the region on some aspects of its exotic flora, the inventorization and documentation of its exotic have received a little or negligible attention. There is a lot of scope of these exotic plant species in Kashmir valley, but lack of authentic identification has been as a big hurdle in their scientific management. It is in this backdrop that the present floristic study was carried out to identify, inventorize, document and characterize the exotic plant species grown in the Valley. The present study has revealed that Kashmir Valley grows 317 exotic plant species, which belong to 209 genera in 85 families. Dicotyledons are the largest group, represented by 252 species in 163 genera and 67 families. Monocotyledons comprise 52 species in 37 genera and 13 families. Gymnosperms are the smallest group, with 13 species distributed in 9 genera and 5 families. Asteraceae is the largest family containing 33 exotic species.

• Journal of Forest and Environmental Science
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• v.30 no.4
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• pp.331-341
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• 2014

The present study is an attempt to give an account of the inventory of plant species, phytosociological characteristics of vegetation and pedological characteristics of Rambi Beat Forest under Senchal East Forest Zone, Darjeeling, West Bengal, India. Its plant community were analyzed quantitatively and synthetically. The results reflect dominancy of dicotyledons over monocotyledons in the four studied sites The plant community comprising of 50 plant species belonging to 40 genera of 27 families. Ramhi beat reflected higher diversity of species. Maximum IVI value was recorded by Viola surpense (47.17) in Rambhi forest beat. The Berger parker index and evenness index were found to be highest for Viola surpense, Fragaria nubicola, Pilea umbrosa in Rambhi beat. The soil characteristics of the different pedons revealed alkaline nature of soil in Rambhi beat. Higher levels of soil organic carbon content reflect higher fertility of the soil of Rambhi beat. The response towards soil available nitrogen and phosphate were different among the ten pedons of Rambhi beat. Therefore, proper management and conservative measures needs to be implemented for conservation of bioresources in Senchel wildlife Sanctuary of West Bengal, India.

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.67-81
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• 2001

Traditionally, genetic variability is generated by an extensive crossing program, which is complemented by strict selection to identify useful new recombinants. Plant biotechnology offers many opportunities for breeders to solve certain breeding problems at the molecular level. The tissue culture methodology and the genetic modification of economically important monocotyledons have undergone a revolution in the last decade. As the production of transgenic plants is a complex procedure, including the uptake of DNA molecules into the cells, the integration of foreign nucleotide sequences into the host genomic DNA and the expression of new genes in a controlled way, and as there are still many unsolved questions, further development is necessary. The methodology opens up the possibility of introducing novel genes that may induce resistance to diseases and abiotic stresses, allow the modification of dough quality and the dietetic quality of proteins, and increase the levels of micronutrients such as iron, zinc, and vitamins. In the present review, the authors would like to summarise the most important advances in wheat transformation.

• Journal of Life Science
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• v.10 no.1
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• pp.52-60
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• 2000

Up to now, there have been done much efforts in regard to nitrate reductase activity (NRA) of dicotyledonous herbs and important crop monocotyledons, but few to wild plants having canopy structure such as Carex. The objective of the present study are to determine: a) the optimum in vivo NR assay conditions for leaf samples of Carex species, b) changes of NRA according to section within leaf and leaf ages, c) diurnal variations. Optimized assay media of each Carex species were determined. NRA of C. rostrata adapted to oligotrophic habitats is readily saturated at lower substrate concentration than those of C. distans and C. gracilis, adapted to meso- and eutrophic habitats, respectively. All Carex species investigated have higher NRA in leaves than in roots. NRA of all species showed maximal values at the middle section of each leaf and in the youngest fully expanded leaves. Compared to C. gracilis, NR in leaves of C. distans was adapted readily to the light period. On the whole, Carex showed rather delayed diurnal variation. Even if the in vivo nitrate reductase assay based on nitrite estimation does not give an accurate estimation of total nitrate reduced, it still serves as a useful tool to find out relative differences in varying environmental conditions. Additionally, in vivo RNA measurements are helpful to understand nitrate reduction and basic nitrogen metabolism of Carex species having different canopy structure.

• Korean Journal of Plant Taxonomy
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• v.50 no.2
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• pp.199-226
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• 2020

Hwangmaesan Mt. (1,108 m) is rich in forest biodiversity and is a part of the Sobaek mountain range located in Hapcheon-gun, Gyeongsang-do in Korea. Unfortunately, the biodiversity and ecosystem integrity of Hwangmaesan Mt. have recently been threatened by heavy human disturbances. We conducted thirteen field surveys between March and October of 2019 to investigate the vascular flora in Hwangmaesan Mt. Through this extensive survey, we found 628 taxa consisting of 106 families, 349 genera, 554 species, 16 subspecies, 48 varieties, and 10 forma. Based on a comparative analysis between the present findings and former flora reports, our results showed an increase by 28 percent in the number of taxa observed. Of the 628 taxa, dicotyledons made up the largest group (477 taxa, 76.0%) followed by monocotyledons (120 taxa, 19.1%). Endemic plants consisted of 16 taxa, with rare plants designated by the Korea Forest Service numbering 14 taxa. Ten taxa were listed on the Red list by the Ministry of the Environment. The floristic target species designated by the Ministry of the Environment numbered 75 taxa, whereas there were 42 taxa of invasive plants.

• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.251-257
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• 2011

Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at $25^{\circ}C$, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.37-42
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• 2013

Rice is one of the most important cereal crops as a model plant for functional genomics of monocotyledons and usually transformed using Agrobacterium tumefaciens. However, the transformation's process using previous method is still time consuming and uneconomical, low efficiency. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the infection and co-cultivation, Agrobacterium elimination, infected calli's selection steps using liquid media. We directly inoculated Agrobacterium containing a ZjLsL gene under the control of constitutive promoter into the 1- to 3-week-old rice calli derived from mature seeds. After 3 days of co-cultivation, the infected calli were transferred onto liquid media of Agrobacterium elimination and calli's selection for 3 days. The calli were transferred to calli's growth solid media for 14 days and then the calli transferred to shoot induction and root induction media. Putative transformants were initially selected on the medium containing phosphinothricin, and the PAT protein verified by PAT strip test. This method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.252-260
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• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.