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Evaluation of liquid and powdered forms of polyclonal antibody preparation against Streptococcus bovis and Fusobacterium necrophorum in cattle adapted or not adapted to highly fermentable carbohydrate diets

  • Cassiano, Eduardo Cuellar Orlandi;Perna, Flavio Junior;Barros, Tarley Araujo;Marino, Carolina Tobias;Pacheco, Rodrigo Dias Lauritano;Ferreira, Fernanda Altieri;Millen, Danilo Domingues;Martins, Mauricio Furlan;Pugine, Silvana Marina Piccoli;de Melo, Mariza Pires;Beauchemin, Karen Ann;Meyer, Paula Marques;Arrigoni, Mario de Beni;Rodrigues, Paulo Henrique Mazza
    • Animal Bioscience
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    • v.34 no.1
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    • pp.74-84
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    • 2021
  • Objective: Feed additives that modify rumen fermentation can be used to prevent metabolic disturbances such as acidosis and optimize beef cattle production. The study evaluated the effects of liquid and powdered forms of polyclonal antibody preparation (PAP) against Streptococcus bovis and Fusobacterium necrophorum on rumen fermentation parameters in ruminally cannulated non-lactating dairy cows that were adapted or unadapted to a high concentrate diet. Methods: A double 3×3 Latin square design was used with three PAP treatments (control, powdered, and liquid PAP) and two adaptation protocols (adapted, unadapted; applied to the square). Adapted animals were transitioned for 2 weeks from an all-forage to an 80% concentrate diet, while unadapted animals were switched abruptly. Results: Interactions between sampling time and adaptation were observed; 12 h after feeding, the adapted group had lower ruminal pH and greater total short chain fatty acid concentrations than the unadapted group, while the opposite was observed after 24 h. Acetate:propionate ratio, molar proportion of butyrate and ammonia nitrogen concentration were generally greater in adapted than unadapted cattle up to 36 h after feeding. Adaptation promoted 3.5 times the number of Entodinium protozoa but copy numbers of Streptococcus bovis and Fibrobacter succinogens genes in rumen fluid were not affected. However, neither liquid nor powdered forms of PAP altered rumen acidosis variables in adapted or unadapted animals. Conclusion: Adaptation of cattle to highly fermentable carbohydrate diets promoted a more stable ruminal environment, but PAP was not effective in this study in which no animal experienced acute or sub-acute rumen acidosis.

Accuracy of dies fabricated by various three dimensional printing systems: a comparative study (다양한 삼차원 프린팅 시스템으로 제작된 다이의 정확도 비교)

  • Baek, Ju Won;Shin, Soo-Yeon
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.4
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    • pp.242-253
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    • 2020
  • Purpose: The aim of this study was to compare the accuracy of dies fabricated using 3D printing system to conventional method and to evaluate overall volumetric changes by arranging the superimposed surfaces. Materials and Methods: A mandibular right first molar from a dental model was prepared, scanned and fabricated with composites of polyetherketoneketone (PEKK). Master dies were classified into 4 groups. For the conventional method, the impression was taken with polyvinylsiloxane and the impression was poured with Type IV dental stone. For the 3D printing, the standard die was scanned and converted into models using three different 3D printers. Each of four methods was used to make 10 specimens. Scanned files were superimposed with the standard die by using 3D surface matching software. For statistical analysis, Kruskal-Wallis test and Mann-Whitney U test were done (P < 0.05). Results: Compared to the standard model, the volumetric changes of dies fabricated by each method were significantly different except the models fabricated by conventional method and 3D printer of Stereolithography (P < 0.05). The conventional dies showed the lowest volumetric change than 3D printed dies (P < 0.05). 3D printed dies fabricated by Stereolithography showed the lowest volumetric change among the different 3D printers (P < 0.05). Conclusion: The conventional dies were more accurate than 3D printed dies, though 3D printed dies were within clinically acceptable range. Thus, 3D printed dies can be used for fabricating restorations.

Implant-assisted removable partial denture using digital guide surgery in partially edentulous mandible: A case report (하악 부분 무치악 환자에서 디지털 가이드 수술을 이용한 임플란트 융합 가철성 국소의치 수복 증례)

  • Kim, Taehoon;Jeong, Chang-Mo;Yun, Mi-Jung;Lee, So-Hyoun;Lee, Hyeonjong;Huh, Jung-Bo
    • The Journal of Korean Academy of Prosthodontics
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    • v.59 no.1
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    • pp.88-96
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    • 2021
  • Fixed implant prostheses or removable partial dentures are common treatment for partially dentulous patients. Recently, an implant-assisted removable partial denture (IARPD) has been introduced and is widely used. In the case of removable partial dentures using implant attachment and surveyed fixed prostheses, the consideration about parallelism of insertion path between implants and surveyed fixed prostheses is an important factor for success of IARPDs. In this case, she complained of discomfort while using the mandibular removable partial denture. Thus, an IARPD using implant surveyed fixed prostheses was fabricated. Thereafter, more implants were placed by a digital guide surgery to have the same insertion path as the existing surveyed fixed prostheses. Locator attachments were installed to the left and right premolar implants, and the left molar was able to obtain support of the removable partial denture with healing abutment. The clinical results were satisfactory on the aspect of aesthetic and masticatory function.

Synthesis of SiO2/Ag Core-shell Nanoparticles for Conductive Paste Application (SiO2/Ag 코어-쉘 나노입자의 합성 및 전도성 페이스트 적용)

  • Sim, Sang-Bo;Han, Jong-Dae
    • Applied Chemistry for Engineering
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    • v.32 no.1
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    • pp.28-34
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    • 2021
  • SiO2/Ag core-shell nanoparticles were synthesized by combining modified Stöber process and reverse micelle method using acetoxime as a reducing agent in water/dodecylbenzenesulfonic acid (DDBA)/cyclohexane reverse micells. The SiO2/Ag core-shells were studied for structure, morphology and size using UV-visible spectroscopy, XRD, SEM and TEM. The size of a SiO2/Ag core-shell could be controlled by changing the [water]/[DDBA] molar ratio (WR) values. The size and the polydispersity of SiO2/Ag core-shells increased with increase of the WR value. The resultant Ag nanoparticles exhibit a strong surface plasmon resonance (SPR) peak at 430 nm over the amorphous SiO2 nanoparticles. The SPR peak shifted to the red side with increase in nanoparticle size. Conductive pastes with 70 wt% SiO2/Ag core-shell were prepared, and the pastes were coated on the PET films using a screen-printing method. The printed paste film of the SiO2/Ag core-shell showed higher surface resistance than the commercial Ag paste in the range of 460~750 µΩ/sq.

Application of CFD to Design Procedure of Ammonia Injection System in DeNOx Facilities in a Coal-Fired Power Plant (석탄화력 발전소 탈질설비의 암모니아 분사시스템 설계를 위한 CFD 기법 적용에 관한 연구)

  • Kim, Min-Kyu;Kim, Byeong-Seok;Chung, Hee-Taeg
    • Clean Technology
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    • v.27 no.1
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    • pp.61-68
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    • 2021
  • Selective catalytic reduction (SCR) is widely used as a method of removing nitrogen oxide in large-capacity thermal power generation systems. Uniform mixing of the injected ammonia and the inlet flue gas is very important to the performance of the denitrification reduction process in the catalyst bed. In the present study, a computational analysis technique was applied to the ammonia injection system design process of a denitrification facility. The applied model is the denitrification facility of an 800 MW class coal-fired power plant currently in operation. The flow field to be solved ranges from the inlet of the ammonia injection system to the end of the catalyst bed. The flow was analyzed in the two-dimensional domain assuming incompressible. The steady-state turbulent flow was solved with the commercial software named ANSYS-Fluent. The nozzle arrangement gap and injection flow rate in the ammonia injection system were chosen as the design parameters. A total of four (4) cases were simulated and compared. The root mean square of the NH3/NO molar ratio at the inlet of the catalyst layer was chosen as the optimization parameter and the design of the experiment was used as the base of the optimization algorithm. The case where the nozzle pitch and flow rate were adjusted at the same time was the best in terms of flow uniformity.

Does dexmedetomidine combined with levobupivacaine in inferior alveolar nerve blocks among patients undergoing impacted third molar surgery control postoperative morbidity?

  • Patil, Shweta Murlidhar;Jadhav, Anendd;Bhola, Nitin;Hingnikar, Pawan;Kshirsagar, Krutarth;Patil, Dipali
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.22 no.2
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    • pp.145-153
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    • 2022
  • Background: Postoperative analgesia (POA) is an important determinant of successful treatment. Dexmedetomidine (DEX) has recently gained attention as a promising adjuvant to local anesthetics (LA). The present study aimed to evaluate the efficacy and safety of levobupivacaine (LB) as an adjuvant during inferior alveolar nerve block (IANB) in the extraction of lower impacted third molars (LITM). Methods: A prospective, randomized, placebo-controlled, triple-blind, parallel-arm, and clinical study was performed on 50 systemically healthy participants who required removal of an asymptomatic LITM. Using a 1:1 distribution, the participants were randomized into two groups (n = 25). Group L (control group) received 1.8 mL of 0.5% LB and 0.2 mL normal saline (placebo) and Group D (study group) received a blend of 1.8 mL of 0.5% LB and 0.2 mL (20 ㎍) DEX. The primary outcome variable was the duration of POA and hemodynamic stability, and the secondary variable was the total number of analgesics required postoperatively for up to 72 h. The participants were requested to record the time of rescue analgesic use and the total number of rescue analgesics taken. The area under the curve was plotted for the total number of analgesics administered. The pain was evaluated using the visual analog scale. Data analysis was performed using paired students and unpaired t-test, Mann-Whitney U test, Chi-square test, and receiver operating characteristic analysis. Statistical significance was set at P < 0.05. Results: The latency, profoundness of anesthesia, and duration of POA were statistically significant (P < 0.05). The differences between mean pain scores at 6, 12, 24, 48, and 72 h were found to be significant (each P = 0.0001). Fewer analgesics were required by participants in group D (2.12 ± 0.33) than in L (4.04 ± 0.67), with a significant difference (P = 0.0001). Conclusion: Perineurally administered LA with DEX is a safe, effective, and therapeutic approach for improving latency, providing profound POA, and reducing the need for postoperative analgesia.

Traumatic Bone Cyst in the Mandibular Symphysis : Case Reports (하악 결합 부위에 발생한 외상성 골낭 : 증례보고)

  • Sung, Minah;Lee, Nanyoung;Lee, Sangho;Jih, Myeongkwan
    • Journal of the korean academy of Pediatric Dentistry
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    • v.48 no.4
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    • pp.476-483
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    • 2021
  • Traumatic bone cyst (TBC) is an asymptomatic lesion seen most in adolescents. TBC is found incidentally on routine panoramic examinations and appears as a relatively well-demarcated unilocular radiolucency with scalloped margins. Histological examination reveals a vacant cavity of cancellous bone usually unlined or very occasionally lined with a thin connective tissue layer. The lack of lining epithelial membrane is common histological feature. The most affected site is between the mandibular canine and third molar. The involvement of the mandibular symphysis is rare. The etiopathogenesis of the TBC is unclear. TBC is treated with surgical exploration and curettage; new bone is formed in place of the lesion within 6 - 12 months of surgery. Diagnosis of TBC prior to surgical intervention has limitations in most of the cases. Both of our patients were diagnosed through radiological examination and biopsy. Neither patient had a history of trauma. After surgery, the panoramic radiograph and CBCT were used to confirm bone healing.

Comparison of Reproducibility of Linear Measurements on Digital Models among Intraoral Scanners, Desktop Scanners, and Cone-beam Computed Tomography

  • Jo, Deuk-Won;Kim, Mijoo;Kim, Reuben H.;Yi, Yang-Jin;Lee, Nam-Ki;Yun, Pil-Young
    • Journal of Korean Dental Science
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    • v.15 no.1
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    • pp.1-8
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    • 2022
  • Purpose: Intraoral scanners, desktop scanners, and cone-beam computed tomography (CBCT) are being used in a complementary way for diagnosis and treatment planning. Limited patient-based results are available about dimensional reproducibility among different three-dimensional imaging systems. This study aimed to evaluate dimensional reproducibility among patient-derived digital models created from an intraoral scanner, desktop scanner, and two CBCT systems. Materials and Methods: Twenty-nine arches from sixteen patients who were candidates for implant treatments were enrolled. Different types of CBCT systems (KCT and VCT) were used before and after the surgery. Polyvinylsiloxane impressions were taken on the enrolled arches after the healing period. Gypsum casts were fabricated and scanned with an intraoral scanner (CIOS) and desktop scanner (MDS). Four test groups of digital models, each from CIOS, MDS, KCT, and VCT, respectively, were compared to the reference gypsum cast group. For comparison of linear measurements, intercanine and intermolar widths and left and right canine to molar lengths were measured on individual gypsum cast and digital models. All measurements were triplicated, and the averages were used for statistics. Bland-Altman plots were drawn to assess the degree of agreement between each test group with the reference gypsum cast group. A linear mixed model was used to analyze the fixed effect of the test groups compared to the reference group (α=0.05). Result: The Bland-Altman plots showed that the bias of each test group was -0.07 mm for CIOS, -0.07 mm for MDS, -0.21 mm for VCT, and -0.25 mm for KCT. The linear mixed model did not show significant differences between the test and reference groups (P>0.05). Conclusion: The linear distances measured on the digital models created from CIOS, MDS, and two CBCT systems showed slightly larger than the references but clinically acceptable reproducibility for diagnosis and treatment planning.

Bioactivity enhancement of zirconia substrate by surface coating of diopside bioceramics using sol-gel method (솔젤법에 의한 다이옵사이드 생체 세라믹의 표면코팅 및 지르코니아 기판의 생체활성 증진)

  • Park, Hyunjung;Lee, Jong Kook
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.32 no.5
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    • pp.183-190
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    • 2022
  • Diopside (CaMgSi2O6) is known to have high bioactivity as well as excellent mechanical properties. In this study, we tried to improve the bioactivity of zirconia ceramics by surface coating of diopside and its bioactivity was investigated through an in vitro test. Surface coating on zirconia substrate was prepared by sol-gel method using a diopside sol which was prepared by dissolving Ca(NO3)2·4H2O, MgCl2·6H2O and Si(OC2H5)4 in ethanol with a fixed molar ratio and then hydrolysis. To examine the bioactivity of diopside coating, we examined the surface dissolution and the precipitation of new hydroxyapatite particles through in vitro test in SBF (Simulated Body Fluid) solution. Dense and thick diopside coating layers could be fabricated on zirconia substrate by sol-gel method. Also, we confirmed that they contained high bioactivity from the in vitro test, indicated the precipitation of hydroxyapatite particles after the 14 days immersion in SBF solution. In addition, we checked that the bioactivity of diopside coated layers was dependent on the repeated coating cycle and coating thickness.

A Study on the Leaching Effect and Selective Recovery of Lithium Element by Persulfate-based Oxidizing Agents from Waste LiFePO4 Cathode (과황산계 산화제에 따른 폐LiFePO4 양극재에서 리튬의 침출 효과와 선택적 회수에 대한 연구)

  • Kim, Hee-Seon;Kim, Dae-Weon;Jang, Dae-Hwan;Kim, Boram;Jin, Yun-Ho;Chae, Byung-Man;Lee, Sang-Woo
    • Resources Recycling
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    • v.31 no.4
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    • pp.40-48
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    • 2022
  • In waste lithium iron phosphate (LFP) batteries, the cathode material contains approximately 4% lithium. Recycling the constituent elements of batteries is important for resource circulation and for mitigating the environmental pollution. Li contained in the waste LFP cathode powder was selectively leached using persulfate-based oxidizing agents, such as sodium persulfate, potassium persulfate, and ammonium persulfate. Leaching efficiency and waste LFP powder properties were compared and analyzed. Pulp density was used as a variable during leaching, which was performed for 3 h under each condition. The leaching efficiency was calculated using the inductively coupled plasma (ICP) analysis of the leachate. All types of persulfate-based oxidizing agents used in this study showed a Li leaching efficiency over 92%. In particular, when leaching was performed using (NH4)2S2O8, the highest Li leaching percentage of 93.3% was observed, under the conditions of 50 g/L pulp density and an oxidizing agent concentration of 1.1 molar ratio.