• The Journal of Korean Medicine
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• v.25 no.3
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• pp.123-136
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• 2004

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won (WC) on the in vitro neuronal development and alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E/sub 18/ rat cortical cells were grown in a neurobasal medium containing B27 supplement and various concentration of WC. Initial development of growth cone was investigated by phase-contrast microscopy, while dendritic spine formation and synaptogenesis were investigated by immunocytochemistry with SynGAPα(a postsynaptic marker) and synaptophysin (presynaptic marker) antibodies. Alteration in gene expression was analyses by microarray using rat 5K-TwinChips. Results : WC suppressed the development of growth cones and WC increased the number of dendritic spines at 20 and 50㎍/mL concentration but there was no statistical significance. Instead, it significantly decreased the number at 100㎍/mL. The expression of anti-apoptosis gene Bcl2-like 1 (Bcl211) increased (Global M=0.46), while Akt1 decreased. Proapoptosis genes Bad and PDCD2 increased. The expression of hemoglobin alpha 1 (probably neuroglobin) increased (Global M=0.93). The expression of antioxidants such as catalase, heme oxygenase (HO), and PRKAG2 gene increased. The expression PKC gene increased. The expression of retinoic acid receptor alpha (RARα) increased significantly (Global M=1.0). Conclusions : These data suggest that WC trends to suppress cellular activity slightly in normoxia and increases the expression of apoptosis-, antioxidation-, oxygen capture-related genes in hypoxia, but increases Bcl111 that anti-apoptosis gene, on the other hand increases Bad, PDCD2 that pro-apoptosis genes, too..

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1975-1983
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• 2008

The neuropeptide ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH) has anti-inflammatory property by down regulating the expressions of proinflammatory cytokines. Because ${\alpha}$-MSH elicits the anti-inflammatory effect in various inflammatory disease models, we examined the therapeutic effect of oral administration of recombinant Lactobacillus casei, which secretes ${\alpha}$-MSH (L. casei-${\alpha}$-MSH), on dextran sulfate sodium (DSS)-induced colitis in Balb/c mice. Thus, we constructed the ${\alpha}$-MSH-secreting Lactobacillus casei by the basic plasmid, pLUAT-ss, which was composed of a PldhUTLS promoter and ${\alpha}$-amylase signal sequence from Streptococcus bovis strain. Acute colitis was induced by oral administration of 5% DSS in drinking water for 7 days. To investigate the effect of L. casei-${\alpha}$-MSH on the colitis, L. casei or L. casei-${\alpha}$-MSH was orally administered for 7 days and their effects on body weight, mortality rate, cytokine production, and tissue myeloperoxidase (MPO) activity were observed. Administration of L. casei-${\alpha}$-MSH reduced the symptom of acute colitis as assessed by body weight loss (DSS alone: $14.45{\pm}0.2\;g$; L. casei-${\alpha}$-MSH: $18.2{\pm}0.12\;g$), colitis score (DSS alone: $3.6{\pm}0.4$; L. casei-${\alpha}$-MSH: $1.4{\pm}0.6$), MPO activity (DSS alone: $42.7{\pm}4.5\;U/g$; L. casei-${\alpha}$-MSH: $10.25{\pm}0.5\;U/g$), survival rate, and histological damage compared with the DSS alone mice. L. casei-${\alpha}$-MSH-administered entire colon showed reduced in vitro production of proinflammatory cytokines and $NF-{\kappa}B$ activation. The ${\alpha}$-MSH-secreting recombinant L. casei showed significant anti-inflammatory effects in the murine model of acute colitis and suggests a potential therapeutic role for this agent in clinical inflammatory bowel diseases.

• Journal of KIISE:Information Networking
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• v.32 no.3
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• pp.318-326
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• 2005

This paper presents the image feature extraction method and the image classification technique to select the harmful image flowed from the Internet by grade of image contents such as harmlessness, sex-appealing, harmfulness (nude), serious harmfulness (adult) by the characteristic of the image. In this paper, we suggest skin area detection technique to recognize whether an input image is harmful or not. We also propose the ROI detection algorithm that establishes region of interest to reduce some noise and extracts harmful degree effectively and defines the characteristics in the ROI area inside. And this paper suggests the multiple-SVM training method that creates the image classification model to select as 4 types of class defined above. This paper presents the multiple-SVM classification algorithm that categorizes harmful grade of input data with suggested classification model. We suggest the skin likelihood image made of the shape information of the skin area image and the color information of the skin ratio image specially. And we propose the image feature vector to use in the characteristic category at a course of traininB resizing the skin likelihood image. Finally, this paper presents the performance evaluation of experiment result, and proves the suitability of grading image using image feature classification algorithm.

• Science of Emotion and Sensibility
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• v.12 no.1
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• pp.109-120
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• 2009

This study was carried out in order to investigate color sensation and sensibility for yellowish natural dye fabrics and reddish ones and to establish prediction models for color sensibility factors of them by color sensation and the related physical measurements focusing on 40s middle-aged people. Eight fabric stimuli which were dyed with a variety of yellowish or reddish natural dyes was subjectively evaluated in terms of color sensation and sensibility by 40s aged participants. As results, three color sensibility factors including 'Active', 'Characteristic', and 'Relax' were extracted and they were examined in respect of their relationships with color sensation and physical color properties. Color sensibility factor 'Active', the dominant factor for the naturally dyed fabrics was explained by $L^*$ and sensation 'Deep' in its predictive model and a yellowish fabric dyed with 300% solution of armur cork unmordanted was perceived the strongest in the factor. Factor 'Characteristic' was predicted by both $a^*$ and sensation 'Light' and reddish natural dye fabrics tended to be felt more strongly for it. Color sensation 'Strong' was the only predictor for factor 'Relax' in that naturally dyed fabrics with lower values for the sensation seemed to show higher 'Relax' factor and a reddish fabric dyed with safflower 125% was the highest for the color sensibility factor. These results could be utilized to design color-sensible natural dye fabrics for middle-aged people.

• Journal of Nuclear Fuel Cycle and Waste Technology
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• v.1 no.1
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• pp.49-55
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• 2013

To characterize quantitatively the transport of $^{99}Tc$ and the global fallout ($^{137}Cs$, $^{90}Sr$, and $^{239+240}Pu$) for soils in Korea, the transport parameters of a convective-dispersion model, apparent migration velocity, and apparent dispersion coefficient were estimated from the vertical depth profiles of the radionuclides in soils. The vertical profiles of $^{99}Tc$ were measured from a pot experiment for paddy soil that had been sampled from a rice-field around the Gyeongju radioactive waste repository in Korea, and the vertical depth distributions of the global fallout $^{137}Cs$, $^{90}Sr$, and $^{239+240}Pu$ were measured from the soil samples that were taken from local areas in Korea. The front edge of the $^{99}Tc$ profiles reached a depth of about 12 cm in 138 days, indicating a faster movement than the fallout radionuclides. A weak adsorption of $^{99}Tc$ on the soil particles by the formation of Tc(VII) and a high water infiltration velocity seemed to have controlled the migration of $^{99}Tc$. The apparent migration velocity and dispersion coefficient of $^{99}Tc$ for the disturbed paddy soil were 2.88 cm/y and 6.3 $cm^2/y$, respectively. The majority of the global fallout $^{137}Cs$, $^{90}Sr$, and $^{239+240}Pu$ were found in the top 20 cm of the soils even after a transport of about 30 years. The transport parameters for the global fallout radionuclides were 0.01-0.1cm/y ($^{137}Cs$), 0.09-0.13cm/y ($^{90}Sr$), and 0.09-0.18cm/y ($^{239+240}Pu$) for the apparent migration velocity: 0.21-1.09 $cm^2/y$ ($^{137}Cs$), 0.12-0.7$cm^2/y$ ($^{90}Sr$), and 0.09-0.36$cm^2/y$ ($^{239+240}Pu$) for the apparent dispersion coefficient.

• Journal of Biomedical Engineering Research
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• v.18 no.4
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• pp.457-466
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• 1997

We developed a new hearing aid telephone which helps the hearing impaired person to improve the listening ability of telephone speech. Recently, the hearing impaired person and the elderly who has hearing loss have been continuously increased and their desire for participating society as a producer has been increased also. So they strong1y want the hearing aid devices which make compensation fortheir handicap. The hearing aid telephone is one of the basic aid devices that helps the hearing impaired to communicate well with other poeple and to acquire easily useful information through the phone. We analyze the hearing ability of the hearing impaired, design the new model of the hearing aid telephone and test the telephone in three fields-electrical, word perception, user test. Our new tolephone has lour band pass filter channels and the center frequencies of these filters are 500, 1000, 2000, 3000Hz which are considered psychoacoustic factors and telephone line characteristics. The hearing impaired can adjust the total gain characteristics of receiving sound to his hearing ability by setting four volumes in the telelphone. This procedure is called fitting which is a very important factor for the hearing impaired to take meaning of speech. The total gain of this telephone is over 20dB from 250Hz to 3200Hz range. From the results of the tests we certify that our new model is better for the hearing impaired to understand the meaning or telephone speech than the old general models. The next step of developing the hearing aid telephone is to study about compressing sidetone and noise, dividing frequency bands, selecting hearing aid pattern and compensating psychoacoustic loudness. we expect that the advanced hearing aid telephone can be developed by the research about speech perception characteristics of the hearing impaired in engineering and clinical side.

• Steel and Composite Structures
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• v.34 no.1
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• pp.1-15
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• 2020

This paper presents experimental and numerical studies on effects of local damages on the in-plane elastic-plastic buckling and strength of a fixed parabolic steel tubular arch under a vertical load distributed uniformly over its span, which have not been reported in the literature hitherto. The in-plane structural behaviour and strength of ten specimens with different local damages are investigated experimentally. A finite element (FE) model for damaged steel tubular arches is established and is validated by the test results. The FE model is then used to conduct parametric studies on effects of the damage location, depth and length on the strength of steel arches. The experimental results and FE parametric studies show that effects of damages at the arch end on the strength of the arch are more significant than those of damages at other locations of the arch, and that effects of the damage depth on the strength of arches are most significant among those of the damage length. It is also found that the failure modes of a damaged steel tubular arch are much related to its initial geometric imperfections. The experimental results and extensive FE results show that when the effective cross-section considering local damages is used in calculating the modified slenderness of arches, the column bucking curve b in GB50017 or Eurocode3 can be used for assessing the remaining in-plane strength of locally damaged parabolic steel tubular arches under uniform compression. Furthermore, a useful interaction equation for assessing the remaining in-plane strength of damaged steel tubular arches that are subjected to the combined bending and axial compression is also proposed based on the validated FE models. It is shown that the proposed interaction equation can provide lower bound assessments for the remaining strength of damaged arches under in-plane general loading.

• Journal of the Korean association of regional geographers
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• v.22 no.2
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• pp.307-330
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• 2016

This article aims to explain how Gangnam, as a model and standard of compressed urbanization in South Korea, was created. Gangnam and Gangnam-style urbanization need attention not only because they contrast with Korea's urbanization in the past as well as urbanization in the West but also they provide an important model in contemporary Korea's politics, economy and culture. However, there are little studies of how Gangnam's peculiar urbanism was created. To fill this gap, this article will first capture Gangnam's peculiar urbanism as a material landscape and sociocultural lifestyle. Gangnam-style urbanism is (a) materially characterized by high-rise apartment complexes owned by the middle and upper class for dwelling and asset growth and (b) socio-culturally characterized by political conservatism, public indifference, competition over academic performance, appearance, and fashion, and nightlife. Then it will show Gangnam's archetype was created in a spatially and temporally compressed way in and through the spatial selectivity of Korean anti-communist authoritarian developmental state strategies: (1) anti-communism led to the diffusion and accommodation of the population through apartments in Gangnam in the context of its confrontation with North Korea and the fast-growing population of Seoul; (2) military authoritarianism excluded the low-income class and the urban poor from urban development; and (3) the developmental state adopted selective housing policy which treated construction companies and the middle class preferentially through exceptional zoning and price distortions, promoting the construction of apartment in Gangnam and its resultant uneven development.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.415-422
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• 2001

Objectives: We aimed to identify the dose-dependent inhibitory effects of Yukmijihwang-tang-Hap-Sabaek-san(YMHSB) and Root cortex of Morus alba L.(RCM) on the mRNA expression of Interieukin(IL)-6, IL-S, granulocyte macrophage colony stimulating factor(GM-CSF) involved in the asthma model. Methods: In this study BEAS-2B cell lines, human epithelial cells, were used. These cells were stimulated by tumor necrosis $factor(TNF)-{\alpha},\;IL-1{\beta}$ and histamine for artificial inflammatory expression. ${\beta}-actin$ messenger RNA(mRNA) was used for the internal standard. After each 24 hours of the YMHSB and RCM treatment, total cellular RNAs were collected by treating RNA zol directly on the living cells. Then the transcriptional activities of IL-6, 8 and GM-CSF were measured by RT-PCR with electrophoresis. Results: In the YMHSB study, the mRNA expression of GM-CSF and IL-8 is significantly inhibited compared to that of control group. But the mRNA expression of IL-6 is not significantly inhibited. In the RCM study, the mRNA expression of GM-CSF and IL-S is significantly inhibited compared to that of control group. But the mRNA expression of IL-6 is not significantly inhibited. Conclusions: This study shows that YMHSB and RCM have dose-dependent inhibitory effects on the mRNA expression of IL-S and GMCSF in human epithelial cells. So these herbal medicines may inhibit the inflammatory process of asthma. Advanced studies are required to investigate the mechanisms of inhibition by herbal medicine in the asthma model.

• Journal of Radiation Industry
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• v.5 no.1
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• pp.75-80
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• 2011

This study was to determine the microbiological safety and tensile strength of gammairradiated porcine tendon for the development of safe xenografts. Escherichia coli and Bacillus subtilis were used as model pathogens and inoculated as $10^6{\sim}10^7log$ colonies forming unit $(CFU)g^{-1}$. As model virus from porcine, porcine parvovirus (PPV), bovine viral diarrhoea virus (BVDV) and poliovirus were inoculated as $10^5{\sim}10^6$ tissue culture infectious dose $(TCID)_{50}g^{-1}$ into porcine skin. The $D_{10}$ value of E. coli and B. subtilis was measured as $0.32{\pm}0.082kGy$ and $4.0{\pm}0.312kGy$, respectively. Additionally, the $D_{10}$ values of PPV, BVDV and poliovirus were also shown as $1.75{\pm}0.131kGy$, $3.70{\pm}0.212kGy$ and $6.26{\pm}0.332kGy$, respectively. Gamma irradiation decreased the tensile strength of porcine tendon. Results indicate that microbiological safety of porcine tendon can be improved significantly by gamma irradiation. However, further studies are needed to improve the tensile strength of gamma-irradiated porcine tendon.