The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Inhibitory Effects of Yukmijihwang-tang-Hap-Sabaek-san and Root Cortex of Morus alba L. on the IL-6, IL-8 and GM-CSF mRNA Levels in Human Epithelial Cells

육미지황탕합사백산(六味地黃湯合瀉白散)과 상백피(桑白皮)가 인간 기관지상피세포의 IL-6, IL-8, GM-CSF mRNA level에 미치는 영향

  • Hwang, Woo-Suck (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Heo, Tae-Seok (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Hee-Jae (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Sung-Ki (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Rhee, Hyung-Koo (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ju, Chang-Yeop (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 황우석 (경희대학교 한의과대학 폐계내과학교실) ;
  • 허태석 (경희대학교 한의과대학 폐계내과학교실) ;
  • 정희재 (경희대학교 한의과대학 폐계내과학교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학교실) ;
  • 이형구 (경희대학교 한의과대학 폐계내과학교실) ;
  • 주창엽 (경희대학교 한의과대학 폐계내과학교실)
  • Published : 2001.09.30
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Abstract

Objectives: We aimed to identify the dose-dependent inhibitory effects of Yukmijihwang-tang-Hap-Sabaek-san(YMHSB) and Root cortex of Morus alba L.(RCM) on the mRNA expression of Interieukin(IL)-6, IL-S, granulocyte macrophage colony stimulating factor(GM-CSF) involved in the asthma model. Methods: In this study BEAS-2B cell lines, human epithelial cells, were used. These cells were stimulated by tumor necrosis $factor(TNF)-{\alpha},\;IL-1{\beta}$ and histamine for artificial inflammatory expression. ${\beta}-actin$ messenger RNA(mRNA) was used for the internal standard. After each 24 hours of the YMHSB and RCM treatment, total cellular RNAs were collected by treating RNA zol directly on the living cells. Then the transcriptional activities of IL-6, 8 and GM-CSF were measured by RT-PCR with electrophoresis. Results: In the YMHSB study, the mRNA expression of GM-CSF and IL-8 is significantly inhibited compared to that of control group. But the mRNA expression of IL-6 is not significantly inhibited. In the RCM study, the mRNA expression of GM-CSF and IL-S is significantly inhibited compared to that of control group. But the mRNA expression of IL-6 is not significantly inhibited. Conclusions: This study shows that YMHSB and RCM have dose-dependent inhibitory effects on the mRNA expression of IL-S and GMCSF in human epithelial cells. So these herbal medicines may inhibit the inflammatory process of asthma. Advanced studies are required to investigate the mechanisms of inhibition by herbal medicine in the asthma model.

Keywords

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