• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.5647-5654
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• 2015

Gall bladder cancer (GBC) is a gastro-intestinal cancer with high prevalence among north Indian women. Platelet derived growth factor-B (PDGFB) and human epidermal growth factor receptor-2 (HER2) may play roles in the etiology of GBC through the inflammation-hyperplasia-dysplasia-carcinoma pathway. To study the association of PDGFB and HER2 polymorphisms with risk of GBC, 200 cases and 300 controls were considered. PDGFB +286A>G and +1135A>C polymorphisms were investigated with an amplification refractory mutation system and the HER2 $Ile^{655}Val$ polymorphism by restriction fragment length polymorphism. Significant risk associations for PDGFB +286 GG (OR=5.25) and PDGFB +1135 CC (OR=3.19) genotypes were observed for GBC. Gender wise stratification revealed susceptibility for recessive models of PDGFB +1135A>C (OR=3.00) and HER2 $Ile^{655}Val$ (OR=2.52) polymorphisms among female GBC cases. GBC cases with gall stones were predisposed to homozygous +286 GG and +1135 CC genotypes. Significant risk associations were found for ACIle (OR=1.48), GAVal (OR=1.70), GAIle (OR=2.00) haplotypes with GBC cases and GCIle haplotype with female GBC cases (OR=10.37, P=<0.0001). Pair-wise linkage disequilibrium revealed negative associations among variant alleles. On multi-dimensional reduction analysis, a three factor model revealed significant gene-gene interaction for PDGFB +286A>G, PDGFB +1135A>C and HER2 Ile165Val SNPs with GBC. Protein-protein interaction showed significant association of PDGFB and HER2 with the epidermal growth factor receptor signaling pathway.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.3
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• pp.545-550
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• 2016

The main purpose of this study is to analyze and evaluate the feasibility of ligthweight pavement system with pile foundation on soft soil by laboratory small chamber test. In order to verify the stability of lightweight pavement system, the 1/30 scaled downed model system was tested at lab. The soft soil condition was simulated and group piles for skin friction resistance were used. Within the limited lab test, the settlements of pavement system were 0.86 mm for Case A, 0.70 mm for Case B, and 0.50 mm for Case C. The converted maximum settlement differential settlement were 25.8 mm and 10.8 mm. These values meet the inside of specification of Bridge Design Guide in Korea. The use of lightweight pavement systems on soft soils could be an alternative construction method on soft soils to reduce the challenges of conventional design and constructions.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.7
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• pp.703-708
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• 2013

Conventional total hip joint replacement(THR) surgery requires a long incision and long rehabilitation time. The stem used in THR is inserted into the cancellous bone of the femur where it plays the role of the artificial joint. Minimally invasive surgery(MIS) has been devised to reduce muscle damage to patients. In this study, a mechanical stem was developed on the basis of MISto reduce the incision length through the principle of the gear. The mechanical stem consists of six components. A prototypical model for a mechanical stem was fabricated using an acryl-based polymer, and its workability was confirmed. To actualize the mechanical stem, a three-dimensional Bio-CAD modeling technique was applied. The hip joint area based on computed tomography(CT) was reconstructed. The safety of the mechanical stem by applying more load than the weight of a man under virtual surgery environment conditions was confirmed by finite element analysis.

• The Journal of Korean Medicine
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• v.24 no.2
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• pp.179-192
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• 2003

Objectives : Boyanghwanoh-tang (Buyanhaiwu-tang) has been used as a prescription for stroke, senile and vascular dementia, ischemic brain and heart damage in Oriental traditional medicine. However, there is little known about the mechanism by which the water extracts of Boyanghwanoh-tang (Buyanhaiwu-tang) rescue cells fromthese damages, and little is known about the protective mechanisms of Boyanghwanoh-tang (Buyanhaiwu-tang) on oxidative stress in neuronal cells. Therefore, we have investigated the role of Boyanghwanoh-tang (Buyanhaiwu-tang) on serum and glucose deprived apoptosis in PC12 cells. Methods : PC12 Cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. The viability of cells was measured by MIT assay. We used DNA fragmentation and caspase 1, 2, 3, 6, 9-likeproteases activation assay. Transcriptional activation of NF-kB was assessed by using electrophoretic mobility shift assay. Results : Boyanghwanoh-tang (Buyanhaiwu-tang) rescued PC12 cells from apoptotic death by serum and glucose deprivation in a dose-dependent manner. The nuclear staining of PC12 cells clearly showed that Boyanghwanoh-tang (Buyanhaiwu-tang) attenuated nuclear condensation and fragmentation, which represent typical neuronal apoptotic characteristics. Boyanghwanoh-tang (Buyanhaiwu-tang) also prevents fragmentation of genomic DNA and activation of caspase 3-like protease in serum and glucose deprived PC12 cells. Furthermore, Boyanghwanoh-tang (Buyanhaiwu-tang) reduced the activation of NF-kB by serum and glucose-deprived apoptosis. Conclusions : These findings suggest that serum and glucose deprivation induces reduced glutathione (GSH) depletion, and consequently, apoptosis through endogenously produced reactive oxygen species in PC12 cells. Also, our data indicated that Boyanghwanoh-tang (Buyanhaiwu-tang) has protective effects against the serum and glucose deprived deaths of PC12 cells, which are mediated by the generation of GSH that, in turn, can reduce oxidative stress caused by reactive oxygen species (ROS) such as hydrogen peroxide.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.4
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• pp.221-226
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• 2016

Since arterial disease in the upper extremity is less common than that in the lower extremity, experimental and numerical investigations related to upper extremity have been rarely performed. We created a three-dimensional model of the arteries, larger than approximately 1 mm, in a Korean adult's left hand (from brachial to digital arteries), from 3T magnetic resonance imaging (MRI) data. For the first time, a three-dimensional computational fluid dynamic method was employed to investigate blood flow velocity, blood pressure variation, and wall shear stress (WSS) on this complicated artery system. Investigations were done on physiological blood flows near the branches of radial and deep palmar arch arteries, and ulnar and superficial palmar arch arteries. The flow is assumed to be laminar and the fluid is assumed to be Newtonian, with density and viscosity properties of plasma.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.463-463
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• 2011

농업은 다른 산업과 달리 원천적으로 기후 조건과 변화에 크게 좌우되는 분야로, 기후변화로 인한 영향에 가장 민감한 분야이다. 안정적이고 지속적인 농작물 생산을 위해서는 기후변화가 농업수자원에 미치는 영향에 대하여 정확히 파악하고, 이로 인해 발생할 수 있는 부정적 효과를 최소화하기 위한 연구가 필요하다. 즉 기온 상승, 강수량 및 강우강도 변화 및 일조시간의 변화 등의 기후변화에 따른 농업수자원에 미치는 영향에 대한 평가가 필요하다. 기후변화가 관개용수에 미치는 영향은 기온 상승과 강수량 증가로 크게 나누어 볼 수 있다. 기온상승은 증발산량 증가로 이어져 관개용수 증가의 원인이 된다. 강수량의 증가는 관개용수를 감소의 원인이 될 수 있지만, 계절별 강우 편차 및 강우 강도의 변화는 유효우량의 감소의 원인이 될 수 있다. 이는 관개기의 농업용수의 안정적 공급과 관리의 어려움이 예상되어 결과적으로 농업용수의 안정성을 위협받을 수도 있다. 즉, 전체 수자원에 큰 비중을 차지하고 있는 논벼의 순용수량에 있어서 기후변화가 미치는 영향에 대한 연구가 필요하다. 또한 농업용수개발과 수자원의 효율적인 관리계획 수립을 위해서는 기후변화에 따른 농업용수 수요분석이 이루어져야 한다. 따라서 본 연구에서는 기후변화 시나리오에 따른 순용수량을 산정하고, 이를 빈도분석하여 논벼의 수요량 변화를 분석하고자 하였다. 이를 위하여 이를 위하여 먼저 CGCM3.1 (Coupled Global Climate Model Ver. 3.1) 및 LARS-WG (Long Ashton Research Station Weather Generator)를 이용하여 2011년부터 2100년까지의 A1B, A2 및 B1 시나리오별로 기상자료를 생성하였다. 이를 바탕으로 물수지 방법을 이용하여 논에서의 순용수량을 산정하고, 한발기준 10년빈도 순용수량을 산정하여 논벼의 수요량을 분석하였다. 연구 결과는 향후 기후변화로 인한 농업용수 변화를 파악하고 전망함으로써, 추후 발생할 수 있는 부정적 효과를 최소화하기 위한 대응 전략 및 농업수자원 정책의 기초 자료로 활용될 수 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.1003-1010
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• 2005

A study consisting of 3 trials was designed to assess the relative biological efficacy of DL-methionine (DL-Met) in comparison to liquid methionine hydroxy analogue (MHA-FA) in growing pigs. In trial I a basal diet was supplemented with three graded levels of DL-methionine (0.25, 0.50 and 0.75 g/kg) or liquid MHA-FA (0.0285, 0.0570 and 0.0855 g/kg) on equimolar basis. The basal diet contained 18.3% CP, 0.22% Met and 0.51% Met+Cys, which is below the methionine requirement for weaned pigs between 10 and 20 kg BW according to NRC (1998) but adequate in all other essential nutrients and energy. Using an exponential model, the efficacy of the two methionine sources was estimated from nitrogen retention data obtained in 42 piglets with an initial BW between 11.0 kg (Exp. 1) and 11.7 kg (Exp. 2). In trials II and III, with a total of 192 and 96 pigs, and with an initial BW of 10.6 and 21 kg, respectively, growth response criteria were assessed to determine if in agreement with previous studies in pigs and poultry a biological effectiveness of about 65% on average could be confirmed for liquid MHA-FA in comparison to DL-met. Based on N-retention (trial I) the biological efficacy of liquid MHA-FA on a weight-to-weight basis was calculated to be 62% relative to DL-met. Basically, these results were confirmed using growth response criteria as measures; the results of trial II and III revealed no significant differences in growth performance and feed conversion between treatments indicating that 100 parts of liquid MHA-FA can be replaced by 65 parts of DL-met.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1306-1318
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• 2009

The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes. Two $\alpha$-amylases ScAmy54 and ScAmy43 predicted to play an important role in starch degradation were showed to produce specific oligosaccharides essentially maltotriose that have a considerable commercial interest. Primary structure of the two enzymes was established by N-terminal sequencing, MALDI-TOF masse spectrometry and cDNA cloning. The two proteins have the same N-terminal catalytic domain and ScAmy43 derived from ScAmy54 by truncation of 96 amino acids at the carboxyl-terminal region. Data of genomic analysis suggested that the two enzymes originated from the same $\alpha$-amylase gene and that truncation of ScAmy54 to ScAmy43 occurred probably during S. sclerotiorum cultivation. The structural gene of Scamy54 consisted of 9 exons and 8 introns, containing a single 1,500-bp open reading frame encoding 499 amino acids including a signal peptide of 21 residues. ScAmy54 exhibited high amino acid homology with other liquefying fungal $\alpha$-amylases essentially in the four conserved regions and in the putative catalytic triad. A 3D structure model of ScAmy54 and ScAmy43 was built using the 3-D structure of 2guy from A. niger as template. ScAmy54 is composed by three domains A, B, and C, including the well-known $(\beta/\alpha)_8$ barrel motif in domain A, have a typical structure of $\alpha$-amylase family, whereas ScAmy43 contained only tow domains A and B is the first fungal $\alpha$-amylase described until now with the smallest catalytic domain.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.446-459
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• 2000

This study was carried out in order to find out an effective machinery utilization strategy by conducting a survey on utilization and maintenance of agricultural machinery. The survey showed that the no. of utilization hours for power tiller in a year was 190.2hrs, 208.6hrs for tractor, 59.1hrs for rice transplanter, 74.0 hrs for combine, 44.6 cultivator and 254.4hrs for 4.4hrs for grain dryer. The period covered the time the machine was until it became unserviceable. The results are as follows: 10.0yrs for power tiller, 7.5yrs for tractor, 7.4yrs for rice transplanter and 5.4yrs for combine. This indicate that the actual period of use for power tiller and rice transplanter was longer than the expected period of duration years so there is a need for adjustment. The factors considered by the farmers for purchasing agricultural machine were: farm size(32%), machine operation (26.0%), performance(l4.0%) and post or after sales service(12.6%), according to the survey. It showed that repair cost rate in a year was classified into major agricultural machine; 4.8% for combine; 3.9% for tractor; 3.5% for rice transplanter; 2.0% for power tiller; 1.6% for grain dryer; and 1.2% for cultivator. The reasons for poor maintenance were insufficient after sales service(25%) and difficulty in buying parts(75%) because of the unavailability of parts in local shops(55%), imported models(30%) and outmoded model(15%).

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.9
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• pp.623-629
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• 2017

Flow structure and mixing characteristics in a micro combustor with a multi-hole baffle were numerically studied using the Reynolds stress model. The multi-hole baffle has geometrical features to produce multiple three-dimensional vortices inside combustion chamber. When the thickness of the baffle's geometrical factors changes, variations of vortical structures occur variously. Among these vortices, the vortex generated from the fuel stream exerts a critical influence on the mixing enhancement. The three-dimensional vortical structure, in its development state, was strongly dependent on the baffle thickness. In particular, as the baffle thickness decreases to values less than the diameter of the fuel hole, the jet stream in baffle holes changes from the parabolic to saddleback profile type. The sizes of recirculation zones inside combustion chamber and the mixing state were closely affected by the structure of the jet streams.