The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Protective Effects of Boyanghwanoh-tang on Serum and Glucose Deprivation-induced Apoptosis of PC12 Cells

보양환오탕이 영양혈청결핍에 의한 PC12 세포의 고사에 미치는 영향

  • 김종길 (원광대학교 한의과대학 심계내과학교실) ;
  • 정승원 (원광대학교 한의학전문대학원) ;
  • 임준모 (원광대학교 한의학전문대학원) ;
  • 장호현 (원광대학교 한의과대학 심계내과학교실) ;
  • 윤종민 (원광대학교 한의학전문대학원) ;
  • 이기상 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의학전문대학원)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Boyanghwanoh-tang (Buyanhaiwu-tang) has been used as a prescription for stroke, senile and vascular dementia, ischemic brain and heart damage in Oriental traditional medicine. However, there is little known about the mechanism by which the water extracts of Boyanghwanoh-tang (Buyanhaiwu-tang) rescue cells fromthese damages, and little is known about the protective mechanisms of Boyanghwanoh-tang (Buyanhaiwu-tang) on oxidative stress in neuronal cells. Therefore, we have investigated the role of Boyanghwanoh-tang (Buyanhaiwu-tang) on serum and glucose deprived apoptosis in PC12 cells. Methods : PC12 Cells have been used extensively as a model for studying the cellular and molecular effects of neuronal cells. The viability of cells was measured by MIT assay. We used DNA fragmentation and caspase 1, 2, 3, 6, 9-likeproteases activation assay. Transcriptional activation of NF-kB was assessed by using electrophoretic mobility shift assay. Results : Boyanghwanoh-tang (Buyanhaiwu-tang) rescued PC12 cells from apoptotic death by serum and glucose deprivation in a dose-dependent manner. The nuclear staining of PC12 cells clearly showed that Boyanghwanoh-tang (Buyanhaiwu-tang) attenuated nuclear condensation and fragmentation, which represent typical neuronal apoptotic characteristics. Boyanghwanoh-tang (Buyanhaiwu-tang) also prevents fragmentation of genomic DNA and activation of caspase 3-like protease in serum and glucose deprived PC12 cells. Furthermore, Boyanghwanoh-tang (Buyanhaiwu-tang) reduced the activation of NF-kB by serum and glucose-deprived apoptosis. Conclusions : These findings suggest that serum and glucose deprivation induces reduced glutathione (GSH) depletion, and consequently, apoptosis through endogenously produced reactive oxygen species in PC12 cells. Also, our data indicated that Boyanghwanoh-tang (Buyanhaiwu-tang) has protective effects against the serum and glucose deprived deaths of PC12 cells, which are mediated by the generation of GSH that, in turn, can reduce oxidative stress caused by reactive oxygen species (ROS) such as hydrogen peroxide.

Keywords

References

  1. 淅江中醫雜誌 v.31 no.8 補陽還五湯加減治療血栓性深靜脈炎63例 葉長靑;蔡傳洋
  2. 醫林改錯 王淸任
  3. 中醫處方解說·臨床·應用 鄭津牟
  4. 方劑學 陳偉;路一平
  5. 東醫處方大全 東醫科學院
  6. 新中醫 v.18 no.8 活血化瘀辨治常見心臟病 劉亦選
  7. Progress in Brain Res. v.63 Selective vulnerability of the hippocampus to ischemia reversible and irreversible types of ischemic cell damage Kirino T.;Tamura A.;Sano K
  8. J. Neurosci. v.15 Mitochondria production of reactive oxygen species in cortical neurons following exposure to N-methyl-D-aspartate Dugan L.L.;Sensi S.L.;Canzoniero L.M.T.;Choi D.W.
  9. Neurosci. Lett. v.120 Reduction of delayed neuronal death by inhibition of protein synthesis Shigeno T.;Yamasaki Y.;Kato G.;Kusaka K.;Mima T.;Takakura K.;Graham D.I.; Eurukawa S.
  10. Curr. Opin. in Neurobial. v.1 Neurofibrillary tangles and beta-amyloid deposits in Alzheimer' disease Goedert M.;Sisodia S.S.;Price D.L.
  11. 中國醫學史 洪元植
  12. 臨床本草學 辛民敎
  13. 申氏本草學(各論) 申佶求
  14. 圖解 鄕藥(生藥)大事典 鄭普燮;辛民敎
  15. 中藥大辭典 김창민;신민교;이경순;안덕균(역)
  16. 中藥大辭典 김창민;신민교;이경순;안덕균(역)
  17. 東醫心系內科學 全國 韓醫科大學 心系內科學敎室(編)
  18. 臨床中風學 金永錫
  19. Brain Res. v.406 Brain neurons develop in a serum and glucose free environment: effects of transferrin, insulin, insulin-like growth factor-Ⅰ and thyroid hormone on neuronal survival, growth and differentiation Aizenman Y;de Velliss J.
  20. J. Biol. Chem. v.272 Epidermal growth factor (EGF)-induced generation of hydrogen peroxide Bae Y.S.;Kang S.W.;Seo M.S.;Bains I.C.;Tekle E;Chock P.B.;Rhee S.G.
  21. Immunol. Today v.15 Oxidative stress as a mediator of apoptosis Buttke, T.M.;Sandstrom, P.A.
  22. Science v.262 Oxidative stress, glutamate and neurodegenerative disorders Coyle, J.T.;P. Puttfarecken
  23. J. Neural. Transm. v.29 no.Suppl. Oxidative stress: a role in the pathogenesis of Parkinson's disease Gotz, M.E.;A. Freyberger;P. Riederer
  24. J. Neurosurgery v.42 Apoptosis in neurological disease Savitz S.I.;Rosenbaum D.M.
  25. J. Biol. Chem. v.271 Induction of CPP32-like activity in PC12 cells by withdrawal of trophic support. Dissociation from apoptosis Greene, L.A.
  26. J. Neurosci. v.48 A mechanism for glutamate toxicity in the C6 glioma cells involving inhibition of cystine uptake leading to glutathione depletion Kato, S.;K. Negishi;K. Mawatari;C.H. Kuo
  27. Neuron v.2 Glutamate toxicity in a neuronal cell line involves inhibition of cysteine transport leading to oxidative stress Murphy, T.H.;M. Miyamoto;A. Sastre;R.L. Schnaar;J.T. Coyle
  28. J. Cereb. Blood Flow Metab. v.8 Protective effect of the glutamate antagonist. MK-801 in focal cerebral ischemia in the cat Ozyurt E.;Graham D.I.;Woodruff G.N.;McCulloch J.
  29. Physiol. Review v.70 Ischemic Cell Death in Brain Neurons Peter Lipton
  30. J. Cell Biol. v.141 The regulation of reactive oxygen species production during programmed cell death Shirlee Tan;Yutaka Sagara;Yuanbin Liu
  31. Science v.270 Requirement for Generation of H2O2 for Platelet Derived Growth Factor Signal Transduction Sundaresan M.;Yu Z.Y.;Ferrans V.J.;Irani K.;Finkel T.
  32. 대한한의학회지 v.21 no.4 還少丹이 영양혈청 결핍성 PC12신경세포의 Apoptosis에 미치는 영향 임준식;김명선;소홍섭;이지현;한상혁;허윤;박래길;문병순
  33. 대한한의학회지 v.23 no.1 PC12 세포의 Apoptosis에 대한 星香正氣散의 방어효과 및 작용기전 연구 최철원;이인;이기상;조남수;문병순
  34. 동의생리병리학회지 v.15 no.5 補陽還五湯이 혈압 및 국소뇌혈류량에 미치는 영향 김남용;이인;장호연;임준모;김승모;조한국;차용석;문병순
  35. 대한한방내과학회지 v.21 no.5 補陽還五湯이 血栓生成關與因子에 미치는 影響 박선영;박종형;한양희;전찬용;김동우;박세기;이청정혜;고승희;고재철;최유경