• Mycobiology
• /
• v.39 no.2
• /
• pp.85-91
• /
• 2011

We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of $25^{\circ}C$ was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, $K_3PO_4$ showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and $K_3PO_4$ (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.

• Environmental Engineering Research
• /
• v.19 no.2
• /
• pp.151-155
• /
• 2014

This study compares the effects of flow on oil and suspended solids removal efficiencies in circular enhanced gravity plate separator equipped with coalescence medium. Coalescence medium acts to capture rising oil droplets and settling solid particles and assist in the coalescence of oil and coagulation of solid. The circular separator uses an upflow center-feed perforated-pipe distributor as the inlet. The co-current flow is achieved using 4 increasing sizes of frustum, whereas cross flow uses inclined coalescence plates running along the radius of the separator. The different arrangement gave the cross flow separator a higher coalescence plan area per operational volume, minimal and constant travelling distance for the oil droplets and particles, lower retention time, and higher operational flowrate. The cross flow separator exhibited 6.04% and 13.16% higher oil and total suspended solids removal efficiencies as compared to co-current flow.

• New & Renewable Energy
• /
• v.17 no.3
• /
• pp.8-15
• /
• 2021

In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 ㎍/L were used in the culture experiments at 0.2, 2, 20, and 100 ㎍/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 ㎍/L biotin and folic acid, respectively. Moreover, Thiaminethiamine--HCl at concentrations of 0.5, 5, 50, and 250 ㎍/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 ㎍/L of thiamine--HCl. As a resultThus, the optimized concentrations of biotin, thiamine--HCl, and folic acid were determined at 0.2, 0.5, and 0.2 ㎍/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.7
• /
• pp.949-954
• /
• 2022

The lipolytic yeast Candida aaseri SH14 contains three Acyl-CoA oxidases (ACOXs) which are encoded by the CaAOX2, CaAOX4, and CaAOX5 genes and catalyze the first reaction in the β-oxidation of fatty acids. Here, the respective functions of the three CaAOX isozymes were studied by growth analysis of mutant strains constructed by a combination of three CaAOX mutations in minimal medium containing fatty acid as the sole carbon source. Substrate specificity of the CaAOX isozymes was analyzed using recombinant C. aaseri SH14 strains overexpressing the respective genes. CaAOX2 isozyme showed substrate specificity toward short- and medium-chain fatty acids (C6-C12), while CaAOX5 isozyme preferred long-chain fatty acid longer than C12. CaAOX4 isozyme revealed a preference for a broad substrate spectrum from C6-C16. Although the substrate specificity of CaAOX2 and CaAOX5 covers medium- and long-chain fatty acids, these two isozymes were insufficient for complete β-oxidation of long-chain fatty acids, and therefore CaAOX4 was indispensable.

• Current Optics and Photonics
• /
• v.6 no.5
• /
• pp.479-488
• /
• 2022

To account for the internal thermal effects of solid-state lasers, a method using a back propagation (BP) neural network integrated with a particle swarm optimization (PSO) algorithm is developed, which is a new wavefront distortion correction technique. In particular, by using a slab laser model, a series of fiber pumped sources are employed to form a controlled array to pump the gain medium, allowing the internal temperature field of the gain medium to be designed by altering the power of each pump source. Furthermore, the BP artificial neural network is employed to construct a nonlinear mapping relationship between the power matrix of the pump array and the thermally induced wavefront aberration. Lastly, the suppression of thermally induced wavefront distortion can be achieved by changing the power matrix of the pump array and obtaining the optimal pump light intensity distribution combined using the PSO algorithm. The minimal beam quality β can be obtained by optimally distributing the pumping light. Compared with the method of designing uniform pumping light into the gain medium, the theoretically computed single pass beam quality β value is optimized from 5.34 to 1.28. In this numerical analysis, experiments are conducted to validate the relationship between the thermally generated wavefront and certain pumping light distributions.

• The Journal of Asian Finance, Economics and Business
• /
• v.10 no.1
• /
• pp.157-167
• /
• 2023

The purpose of this research was to determine the degree to which Small-to-Medium Enterprise (SME) owners and operators in Mountain Province were willing to take on financial risk to invest in the capital markets as a potential additional source of income, as well as the extent to which these five indicator variables-particularly their income, expenses, financial goals, liquid cash, and insurance coverage-were influenced by demographic factors. The study used a quantitative approach and employed a descriptive survey research method. The results show that the SME Owners and Operators in Mountain Province have minimal knowledge of capital market investments which makes them moderate investors with a neutral level of financial risk tolerance toward capital market investment. Their marital status, net income, and educational attainment significantly influence their financial risk tolerance level. The respondents also believe that engaging in the capital markets will grow their businesses. Further, the extent of influence of Income, Expenses, Liquid Cash, and Insurance Cover on the financial risk tolerance of the SME owners and operators in Mountain Province a great extent; thus, making them careful in investing in the capital markets, and it is primarily affected by their Net Income. Consequently, the financial goals of SME owners and operators in Mountain Province have a vital role in their financial risk tolerance level.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.100.1-100
• /
• 2003

A dctA gene encoding a protein with identity to a C4-dicarboxylate/H+ was cloned from a beneficial biocontrol bacterium, P. chororaphis O6. Expression of the dctA was induced in minimal medium by several organic acids and was repressed by glucose. Highest expression was observed in early-log cells grown on fumarate and succinate with decline as cells approached late-log phase. The dctA transcript accumulated weakly when cells were grown on malate but strong expression was observed with benzoate. Expression of the dctA transcript was repressed in early-log cells upon addition of glucose to fumarate, but was detected as the cell culture aged. A dctA-deficient mutant of O6, constructed by marker exchange mutagenesis, did not grow on minimal medium containing succinate, benzoate, or fumarate, and growth on malate was delayed. The dctA mutant and wild type grew equally on glucose. The dctA mutant on cucumber roots in sterilized potting soil was colonized at levels comparable to those of the wild type, but induction level of disease resistance by the mutant against target leaf spot disease was decreased. These results may indicate that the dctA is essential for utilization of certain organic acids and its expression is controlled by the availability of sugars. In addition, the dctA is not essenitial for cucumber root colonization, but important for induction of disease resistance.

• KSBB Journal
• /
• v.29 no.6
• /
• pp.399-404
• /
• 2014

Production of biodegradable polymer polyhydroxyalkanoates (PHAs) from industrial wastes exhibits several advantages such as recycle of waste and the production of high valuable products. To this end, this study aimed at isolating from the sewage treatment plant a PHA producing bacterium capable of utilizing wastes generated from biodiesel and food industries. A Bacillus cereus strain capable of producing poly(3-hydroxybutyrate) [P(3HB)] was isolated, which was followed by confirmation of P(3HB) accumulation by gas-chromatographic analyses. Then, the effects of nutrient limitation on P(3HB) production by B. cereus was first examined. Cells cultured in a minimal medium under the limitation of nitrogen, potassium and sulfur suggested that nitrogen limitation allows the highest P(3HB) accumulation. Next, production of P(3HB) was examined from both waste of biodiesel production (crude glycerol) and waste from food industry (spent coffee grounds). Cells cultured in nitrogen-limited minimal medium supplemented crude glycerol and waste spent coffee grounds extract accumulated P(3HB) to the contents of 2.4% and 1.0% of DCW. This is the first report demonstrating the capability of B. cereus to produce P(3HB) from waste raw materials such as crude glycerol and spent coffee grounds.

• Journal of Korea Soil Environment Society
• /
• v.1 no.1
• /
• pp.39-46
• /
• 1996

For the purpose of development of bioremediation technology for soil contaminated by chlorinated phenols, this study was focused on the isolation and characterization of bacteria capable of degrading chlorinated phenols, the establishment of analytical methods for chlorinated phenols, and the investigation of the contaminated sites. One site near the Incheon Industrial Complex was identified as a pentachlorophenol (PCP)-contaminated spot. The soil brought from the PCP-contaminated site contained 10-100$mu\textrm{g}$/g wet soil of PCP. Many bacterial strains capable of growing on a minimal medium containing PCP were isolated from 15 soil samples collected throughout the land, and among them, 10 active isolates were finally selected for the further studies on the biodegradability and for the use in in situ bioremediation of contaminated soil. These isolates showed species-specific pattern in PCP-decrease and cell growth in a minimal medium containing 500-1,000mg/ιPCP. Strain Bul degraded 90% of PCP at 216 hrs after incubation. Expecially, strain Bu34 was capable of degrading 4,000mg/ι PCP and was identified as Pseudomonas putida Bu34. It is seemed that the isolated active bacteria could be effectively used for the bioremediation of PCP-contaminated sites.

• The Plant Pathology Journal
• /
• v.28 no.4
• /
• pp.439-445
• /
• 2012

The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond formation protein B (dsbB), Clp protease (clp), and polyamine synthase (speD). The chitinase production of the SpeD mutant was restored by the addition of exogenous spermidine or spermine to the bacterial cultures. The speD and clp mutants lost in vitro antifungal activities against plant fungal pathogens. However, the gluP and dsbB mutants showed similar antifungal activities to that of the wild-type. The growth of the mutants in nutrient rich conditions containing chitin was similar with that of the wild-type. However, growth of the speD and gluP mutants was defective in chitin minimal medium, but was observed no growth retardation in the clp and dsbB mutant on chitin minimal medium. In this study, we identified the four genes might be involved and play different role in the production of extracellular chitinase and antifungal activity in L. enzymogenes C-3.