• 미생물학회지
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• 제19권2호
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• pp.63-77
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• 1981

The growth rate of yeast population (Saccharomyces uvarum) cultivated in the Knopp's modified medium (plus various carbon sources) appeared the highest value when the Knopp's minimal medium was treated to 1.5% with disaccharide such as maltose and sucrose. Also the treatment of lactose and raffinose resulted in polulation growth as to the population size in case of maltose and sucrose. However, the gorwth of yeast was not occurred at all when a polysaccharide, such as inulin, was added as carbon source. The growth from of yeast population in Knopp's modified medium are characterized by the fact that log phase continued 100hrs after inoculation and that stationary state phase appeared in general 250hrs after inoculation. Applying the various carbon sources to respiration substrate for yeast cell, the respiration rate of yeast showed the highest value in treatment of maltose and followed in order of raffinose, lactose, glucose, and sucrose. Determined the amount of poly-phosphate and turn over pathway of poly-phosphate according to culture phase of yeast, it is revealed that the yeast synthesized 3 types of poly phosphate (poly-P A,B, and C) and postulated that turn over pathway of poly-phosphate as follows ; Inorganic phosphate is converted into each kind of polyphosphates, and then one part of poly-P-C is converted into poly-P-B, the rest poly-p-C and poly-P-B are converted into poly-P-A. The synthesized poly-phosphate is considered to have a role as energy pool utilizing to synthesis of cellular organic materials. Of the 13 carbon sources used in this experiment, the useful carbon sources for biosynthesis of poly-phosphate and cellular organic materials are confirmed as disaccharide (maltose and sucrose) as well as glucose. Protein synthesis in yeast cell showed the two peaks on 6th and 8th day after inoculation ; nucleic acid on 2nd day (48hrs), carbohydrates on 2nd day (48hrs), and phospholipid on 2nd and 8th day after inoculation, respectively.

• 미생물학회지
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• 제42권2호
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• pp.131-134
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• 2006

Clavicorona pyxidata DGUM 29005의 균사 생육을 위한 최적 배양조건 및 효소 활성을 조사하였다. 균사 생육을 위한 최적 온도 및 pH는 각각 $24^{\circ}C$ 및 5.0이었다. 사용된 복합배지 중 malt extract medium (MEM)에서 가장 좋은 균사 생육을 나타내었다. 최소배지로 Czapek-Dox 한천배지를 사용하고 탄소원으로 trehalose, mannitol, sucrose 및 maltose 첨가시 균사생육이 우수하였다. 전반적으로 무기질소원이 유기질소원 보다 균사 생육을 더 촉진하였으며, 무기질소원으로 calcium nitrate를 사용하였을 때 균사 생육이 가장 우수하였다. 인산원으로 $Na_2HPO_4$를 사용했을 때 균사생육이 촉진되었으며, 가장 우수한 비타민원은 p-aminobenzoic acid이었다. MEM 액체배지를 사용하여 $24^{\circ}C$에서 20일간 C. pyxidata DGUM 29005를 배양하여 균사외 분비효소 및 균사내 효소의 활성도를 측정한 결과, 균사외 분비 효소 중 laccase의 활성도가 다른 효소에 비해 높았으며, ${\alpha}$-amylase, chitinase, lipase 및 pretense의 활성도는 낮거나 없었다.

• Journal of Microbiology
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• 제35권4호
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• pp.271-276
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• 1997

We isolated phenotypic suppressors of an absB (antibiotics synthesis suppression) strain. In the absB colonies, all four antibiotics including two pigmented antibiotics were blocked so that no pigmentation could be found. We assumed that in the colonies with the wuppressive(or reversive) mutation, both pigmentation would be restored so that the strains with suppressive mutation could be cisually detected. Harvested absB spores were treated with chemical mutagen along with electric shock, and were spread on specially fromulated minimal medium plates. The pigmented colonies were isolated from the unpigmented majorities. In one candidate strain, the restoration and significant overproduction of actinorhodin and undecylprodigiosin were recognized. In three other candidate strains, the overproduction of actinorhodin and restoraion of undecylprodigiosin were observed. The production of the two unpigmented antibiotics (CDA and methylenomycin) were visualized in the tested candidate strains. The strains with wuppressive mutations would be very useful in dlucidating the regulation network of antiviotics synthesis and overproduction of the antibiotics.

• Journal of Microbiology and Biotechnology
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• 제7권5호
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• pp.360-362
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• 1997

A transposon Tn5 mutant of Rhodobacter sphaeroides 2.4.1 was isolated for its impaired ability of growth on minimal medium containing ${\beta}$-hydroxybutyric acid as a sole carbon source. The mutant, R. sphaeroides S7 showed approximately 6-fold decrease in ${\beta}$-hydroxybutyrate dehydrogenase activity compared with that of wild type. In R. sphaeroides S7 the Tn5 was located in DNA region corresponding to a 4.2-kb EcoRI DNA fragment of R. sphaeroides 2.4.1 chromosome.

• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.173-176
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• 2001

This report concerns the role of indole-3-acetic acid (IAA) in bacterial pustule disease of soybean. Pustule production in soybean leaves caused by Xanthomonas axonopodis pv. glycines was accompanied by a drastic increase in IAA content of host tissues. The phytopathogenic bacterium synthesized IAA in a tryptophan concentration-dependent manner when grown in a defined minimal medium. In complex media, however, the pathogen showed no response to tryptophan feeding, implying that the bacterial biosynthetic machinery of IAA is strictly regulated by nutrient availability of its growth environments. The results may suggest that IAA of bacterial origin and tryptophan of plant origin be involved in the process of pustule symptom development in soybean.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.497-500
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• 2000

유류오염토양에서 분리한 유류분해균주 중 계면장력 저하능이 우수한 균주를 분리하여 동정한 결과 Pseudomonas aeruginosa D2D2로 판명되었다. 탄소원으로 glucose와 olive oil을 각 1.5%씩 첨가하였을 때 표면장력이 27.9 dyne/cm로 표면장력 저하능력이 가장 우수한 것으로 나타냈다. 생산된 biosurfactant로 ethly acetate 추출하여 crude biosurfactant를 얻었으며, TLC 분석한 결과 glycolipid계열의 biosurfactant임을 확인하였다.

• BMB Reports
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• 제31권3호
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• pp.240-244
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• 1998

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a major virulence factor and in a potent immunogen. In order to express the recombinant urease at a higher level, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a high copy-number vector. The recombinant H. pylori urease expressed in an E. coli strain that was grown in a rich medium supplemented with added nickel was purified to near homogeneity by using DEAE-Sepharose, Superdex HR200, and Mono-Q (FPLC) columns and the purified enzyme possessed the specific activity of 1255 U/mg. Polyclonal antibodies raised against the purified recombinant H. pylori urease were shown to be very specific when subjected to Western blot analysis, in which crude extracts from the H. pylori ATCC strain and the recombinant E. coli strains expressing various bacterial ureases were exnmined for cross-reactivity.

• 디지털산업정보학회논문지
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• 제5권1호
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• pp.23-32
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• 2009

Although the web is becoming a widely accepted medium, it provides relatively poor performance and low availability. A cluster consists of a collection of interconnected stand-alone computers working together and provides a high-availability solution in application area such as web services or information systems. Web server clusters require a high-availability service with a proactive and practical fault management. However, as the system complexity grows, it is not easy to meet the requirement. Therefore, web server clusters must have self-fault management capability for meeting high-availability requirement. In this paper, we propose high availability web server clusters using self-healing technique with a minimal human intervention. Our experimental results show that a proposed method can be used to improve the availability of web server clusters.

• 미생물학회지
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• 제22권1호
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• pp.29-34
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• 1984

서울 근교에서 채취한 河川水 및 土壤標品으로부터 238菌株의 탄화수소자화세균을 分離하였다. 分離된 菌株중 68菌株는 Pseudomonas屬으로 同定되었고, 11菌株는 Alcaligenes屬으로, 10菌株는 Acinetobacter屬으로 同定되었다. 68菌株의 Pseudomona중, 35菌株는 P. aeruginosa, 5菌株는 P. fluorescence, 10菌株는 P. putida, 그리고 2菌株는 P. mendocina로 각각 同定되었다.

• 미생물학회지
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• 제29권5호
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• pp.270-277
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• 1991

A mutational alteration in the signal sequence of ribose-binding protein (RBP) of Escherichia coli, rbsB103, completely blocks the export of the protein to the periplasm. Intragenic suppressors for this mutation have been selected on minimal medium with ribose as a sole carbon source. Six suppressor mutations were characterized in detail and were found to have single amino acid wubstitution in the mature portion of RBP, which resulted in the mobility shift of the proteins on SDS polyacrylamide gel. Amino acid changes of these suppressors were localized in several peptides which are packed to form the N terminal domain of typical bilobate conformation of RBP. The involvement of SecB, a molecular chaperone, was investigated in the suppression of signal sequence mutation. Translocation efficency was found to be increased by the presence of SecB for all suppressors. It is likely that the folding characteristics of RBP altered by the suppressor mutations affect the affinity of interaction between SecB and RBP.