• 영어영문학
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• 제56권6호
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• pp.1339-1360
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• 2010

An analogical reading of socio-historical context of Harold Pinter's The Caretaker employs some postcolonial discursive analyses of postimperial British capitalistic interests in their post war reconstruction. It is also concerned with causes of so-called broken society. The Caretaker dramatizes minimal actions: a tramp is invited by the elder brother; a job as caretaker is offered; he is reluctant to accept the first offer by the elder brother, but is willing to the second by the younger; eventually, he is excluded because he makes noises while dreaming. These trivial actions produce serious and critical speech acts with their socio-historical implications. The tramp Davies is socially and thereby existentially excluded from the centre of the cold, banished to even colder peripheries. The audience face to the question. Why is Davies excluded? This study tries to answer the question, uncovering deep-rooted capitalistic racism, and reading its symptoms. Even after 50 years The Caretaker was staged, post-imperial broken society tries to operate the betrayals of disparity between the cause and effect of what has gone wrong. Pinter confirms that the action of the play takes place in a house in west London. With the city of London as its capitalistic centre, British imperialism lavished much of its wealth which has only served sectional interests dividing people against themselves. Pinter dramatizes the root of broken society. On the one hand, Pinter foregrounds the very general conflicts between individuals and forms of power; on the other hand, he underlies the very specific strategies of socio-historical exploitation, domination and exclusion.

• 한국의학물리학회지:의학물리
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• 제18권2호
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• pp.73-80
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• 2007

제주 해안에서 잡은 60여 개의 군소를 대상으로 한 실험 데이터 중에서 5개의 세포에 대한 실험 결과를 분석해 보니 주목할 만한 결과들이 나왔다. 이들 5개의 세포들 중에 3개의 세포에서는 $32^{\circ}C$에서 $10^{\circ}C$까지 온도를 내리는 동안에 이완된 진동 상태, 연속발화상태 사이의 과분극 기간이 짧은 버스팅, 혼돈 양상의 버스팅, 주기 배가 양상, 연속발화 기간은 길고 휴지 기간은 짧은 버스팅, 일정하게 반복되는 비팅 상태이거나 타원형 버스팅, 휴지상태가 차례차례로 나타났으며, $10^{\circ}C$에서 $32^{\circ}C$까지 온도를 올리는 동안에는 그 반대 순서로 발화 형태가 변화하였다. 같은 온도 범위에서 80분 정도의 주기로 계속해서 열변화 자극을 줄 때마다 이러한 발화 형태 변화는 일정하게 나타났다. 그러나 나머지 두 세포의 경우에는 이와는 다른 발화 형태의 온도에 따른 변화를 보였다. 이들 경우에 이러한 열변화 자극을 24시간 이상 지속하였으나 발화 진폭은 크게 줄어들지 않았다. 그리고 평균발화 진동수, 버스팅 신호 사이의 평균 과분극상태 지속시간, 활동전위 진폭과 활동전위의 최저값 등을 $C^{++}$로 짠 프로그램을 실행시켜서 비교 분석 가능하였다. 온도에 따른 발화 진동수의 분포는 저온과 고온에서는 낮으나 중간 영역에서는 높은 종 모양의 형태를 보였는데, 온도를 내리는 동안에는 온도를 올리는 동안 보다 발화 진동수가 높았다.

• 응용통계연구
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• 제26권1호
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• pp.151-162
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• 2013

중고제품 특히 고가인 중고품에 대한 점증하는 수요로 인하여 그러한 제품에 대한 보증과 보전정책이 최근 제품의 신뢰성을 향상시키기 위해 연구되고 있다. 이 논문에서는 사용한 년 수 x에서 고객에 의해서 구입된 중고제품에 대한 주기적인 유지보수모형을 연구하였다. 구입할 때 판매자는 제품의 고장율을 줄이기 위해서 그리고 각각의 유지보수가 수행되고 난 이후에 신뢰성을 향상시키기 위해서 정해진 보증기간을 제공한다. 만일 연속적인 유지보수 사이에서 고장이 일어난다면 단지 최소수리가 행해진다. 보증정책에 대해서 보증기간동안에 주기적인 유지보수 점검과 더불어 각 고장에 관해서는 무상 비재생수리를 한다. 따라서 이러한 보증정책 하에서 보증기간에 일어난 모든 유지보수와 수리비용은 판매자에게 부과된다. 제안된 주기적인 유비보수 계획에 대해서 보증기간 동안에 판매자에게 부과된 기대 총비용을 계산하기 위한 모형과 판매자의 측면에서 총기대보증비용을 최소화하기 위한 각 유지보수에서 고장율의 최적향상수준을 유도한다. 또한 제안된 방법들에 근거해서 최적향상수준에 대한 수치적인 결과를 제시한다.

• 한국동물위생학회지
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• 제44권3호
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• pp.169-174
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• 2021

Mastitis is an inflammatory condition of the mammary gland, most often caused by bacterial infections, resulting in significant economic losses to the dairy industry. Antimicrobial resistance has been of great concern because of the extensive clinical use of antibiotics. For this reason, the development of new compounds as an alternative treatment to bovine mastitis is needed. Bee venom has been widely used as an oriental treatment for several inflammatory diseases and bacterial infections. The aim of the present study was to evaluate the antimicrobial activity of bee venom on bacteria isolated from bovine mastitis. A total of 107 isolates from bovine mastitic milk samples collected in 2019 and 2020 in Jeonbuk province. All bacterial isolates were tested for susceptibility to bee venom of the honey bee (Apis mellifera). In order to obtain comprehensive antibacterial activities of the bee venom, we measured the minimal inhibitory concentration (MIC) of the bee venom against bacterial strains. Bee venom showed significant inhibition of bacterial growth of Gram-negative bacteria Citrobacter spp., Escherchia coli, Klebsiella spp., Pseudomonas spp., Serratia spp. and Raoultella with MIC values of 96, 81, 72, 230, and 85 ㎍/mL, respectively, and Gram-positive bacterial Enterococcus spp., Staphylococcus spp. and Streptococcus spp. with MIC values of 29, 21 and 16 ㎍/mL, respectively. The results indicated that the MIC values were different depending on the bacterial strains, and those of Gram-positive bacteria were lower than those of Gram-negative bacteria for bee venom. These findings suggested that bee venom could be an effective antimicrobial treatment for bovine mastitis; however, further research is necessary to evaluate the mechanism underlying the antimicrobial action, its effectiveness/safety in vivo and effective application for therapeutic use.

• 한방재활의학과학회지
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• 제25권2호
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• pp.1-13
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• 2015

Objectives Methicillin-Resistant Staphylococcus aureus (MRSA) is a human pathogen and a major cause of hospital-acquired infections. New antibacterial agents that have not been compromised by bacterial resistance are needed to treat MRSA-related infections. In this study, we investigated the antimicrobial activity ofethanol extract of Haedokgeumhwa-san (HGH) which prescription is composed of korean medicine against MRSA. Methods The antibacterial activity of HGH extract was evaluated against MRSA strains by using the Disc diffusion method, broth microdilution method (minimal inhibitory concentration; MIC), checkerboard dilution test, and time-kill test; its mechanism of action was investigated by bacteriolysis, detergent or ATPase inhibitors. The checkerboard dilution test was used to examined synergistic effect of ampicillin, oxacillin, ciprofloxacin, vancomycin, gentamicin and norfloxacin in combination with HGH ethanol extract. A time-kill assay was performed a survival curve which was obtained by plotting viable colony counts depending on time on bacterial growth. Results The minimum inhibitory concentration (MIC) of ethanol extract (HGH) ranged from 1,000 to $2,000{\mu}g/mL$ against all the tested bacterial strains, respectively. We are able to confirm that HGH extract has potentially strong antibacterial activity. In the checkerboard dilution test, fractional inhibitory concentration index of HGH in combination with antibiotics indicated synergy or partial synergism against S. aureus. A time-kill study showed that the growth of the tested bacteria was considerably inhibited after 8 hr of treatment with the combination of HGH with selected antibiotics. For measurement of cell membrane permeability, HGH $250{\sim}1,000{\mu}g/mL$ along with concentration of Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (Tris) were used. In the other hand, N,N-dicyclohexylcarbodimide (DCCD) and Sodium azide ($NaN_3$) was used as an inhibitor of ATPase. TX, Tris, DCCD and $NaN_3$ cooperation against S. aureus showed synergistic action. Accordingly, antimicrobial activity of HGH was affected by cell membrane and inhibitor of ATPase. Conclusions These results suggest that Haedokgeumhwa-san extract has antibacterial activity, and that HGH extract offers a potential as a natural antibiotic against MRSA.

• Journal of Veterinary Science
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• 제22권6호
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• pp.41.1-41.14
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• 2021

Background: Our previously prepared ceftiofur (CEF) hydrochloride oily suspension shows potential wide applications for controlling swine Streptococcus suis infections, while the irrational dose has not been formulated. Objectives: The rational dose regimens of CEF oily suspension against S. suis were systematically studied using a pharmacokinetic-pharmacodynamic model method. Methods: The healthy and infected pigs were intramuscularly administered CEF hydrochloride oily suspension at a single dose of 5 mg/kg, and then the plasma and pulmonary epithelial lining fluid (PELF) were collected at different times. The minimum inhibitory concentration (MIC), minimal bactericidal concentration, mutant prevention concentration (MPC), post-antibiotic effect (PAE), and time-killing curves were determined. Subsequently, the area under the curve by the MIC (AUC_0-24h/MIC) values of desfuroylceftiofur (DFC) in the PELF was obtained by integrating in vivo pharmacokinetic data of the infected pigs and ex vivo pharmacodynamic data using the sigmoid E_max (Hill) equation. The dose was calculated based on the AUC_0-24h/MIC values for bacteriostatic action, bactericidal action, and bacterial elimination. Results: The peak concentration, the area under the concentration-time curve, and the time to peak for PELF's DFC were 24.76 ± 0.92 ㎍/mL, 811.99 ± 54.70 ㎍·h/mL, and 8.00 h in healthy pigs, and 33.04 ± 0.99 ㎍/mL, 735.85 ± 26.20 ㎍·h/mL, and 8.00 h in infected pigs, respectively. The MIC of PELF's DFC against S. suis strain was 0.25 ㎍/mL. There was strong concentration-dependent activity as determined by MPC, PAE, and the time-killing curves. The AUC_0-24h/MIC values of PELF's DFC for bacteriostatic activity, bactericidal activity, and virtual eradication of bacteria were 6.54 h, 9.69 h, and 11.49 h, respectively. Thus, a dosage regimen of 1.94 mg/kg every 72 h could be sufficient to reach bactericidal activity. Conclusions: A rational dosage regimen was recommended, and it could assist in increasing the treatment effectiveness of CEF hydrochloride oily suspension against S. Suis infections.

• 생명과학회지
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• 제32권1호
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• pp.56-62
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• 2022

본 연구에서는 천연물유래 구강건강 개선소재로써 노각나무 가지의 이용 가능성을 검토하기 위해 노각나무 가지 추출물이 치주염 원인균인 P. gingivalis KCTC5352의 병원성인자(virulence factor)에 미치는 영향을 조사하였다. 추출물은 P. gingivalis에 대해 정균작용을 나타내었고 P. gingivalis에 대한 추출물의 MIC는 0.6 mg/ml였다. 추출물 처리구(0.2-2.0 mg/ml)에서 P. gingivalis의 병원성 인자인 바이오필름은 추출물의 농도가 증가할수록 농도 의존적으로 억제되는 경향을 나타내었고, 주사전자현미경으로 추출물 처리구의 P. gingivalis를 관찰한 결과에 의하면 추출물 무처리구에서는 세포들의 응집(autoaggregation)과 바이오필름이 관찰되었지만 추출물 처리구에서는 세포들의 응집과 바이오필름이 관찰되지 않았다. qRT-PCR을 이용하여 P. gingivalis의 바이오필름 형성에 필수적인 섬모(fimbriae) 유전자에 대한 mRNA 발현을 조사한 결과에서도 섬모관련 유전자인 fimA와 fimB에 대한 mRNA 발현은 추출물의 농도가 높아질수록 농도의존적으로 감소되는 것을 확인할 수 있었다. 이상의 결과를 종합하면 노각나무 가지 추출물은 치주염 원인균인 P. gingivalis에 대해 정균작용을 나타낼 뿐만 아니라 P. gingivalis의 병원성 인자인 바이오필름의 형성을 효과적으로 억제할 수 있기 때문에 천연물유래 구강건강 개선소재로써 이용 가능성이 높을 것으로 판단된다.

• Journal of Ginseng Research
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• 제37권3호
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• pp.273-282
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• 2013

The present study was designed to investigate the cardioprotective effects of Korean Red Ginseng extract (KRG) on isoproterenol (ISO)-induced cardiac injury in rats, particularly in regards to electrocardiographic changes, hemodynamics, cardiac function, serum cardiac enzymes, components of the myocardial antioxidant defense system, as well as inflammatory markers and histopathological changes in heart tissue. ISO (150 mg/kg, subcutaneous, two doses administered at 24-hour intervals) treatment induced significant decreases in P waves and QRS complexes (p<0.01), as well as a significant increase in ST segments. Moreover, ISO-treated rats exhibited decreases in left-ventricular systolic pressure, maximal rate of developed left ventricular pressure ($+dP/dt_{max}$) and minimal rate of developed left ventricular pressure ($-dP/dt_{max}$), in addition to significant increases in lactate dehydrogenase, aspartate transaminase, alanine transaminase and creatine kinase activity. Heart rate, however, was not significantly altered. And the activities of superoxide dismutase, catalase and glutathione peroxidase were decreased, whereas the activity of malondialdehyde was increased in the ISO-treated group. ISO-treated group also showed increased caspase-3 level, release of inflammatory markers and neutrophil infiltration in heart tissue. KRG pretreatment (250 and 500 mg/kg, respectively) significantly ameliorated almost all of the parameters of heart failure and myocardial injury induced by ISO. The protective effect of KRG on ISO-induced cardiac injury was further confirmed by histopathological study. In this regard, ISO treatment induced fewer morphological changes in rats pretreated with 250 or 500 mg/kg of KRG. Compared with the control group, all indexes in rats administered KRG (500 mg/kg) alone were unaltered (p>0.05). Our results suggest that KRG significantly protects against cardiac injury and ISO-induced cardiac infarction by bolstering antioxidant action in myocardial tissue.

• Steel and Composite Structures
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• 제36권4호
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• pp.369-381
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• 2020

Nowadays, in prefabricated composite construction, composite action between steel beam and concrete slab is often achieved with positioning of shear connectors in envisaged openings of concrete slabs. Prefabricated concrete slabs are used for composite steel-concrete buildings and bridges, both for the construction of new structures and for renovation of existing ones, significantly reducing construction time. Development of different types of shear connectors represent alternative solution to the traditionally used headed studs, considering their shear resistance, stiffness and ductility. New types of shear connectors tend to reduce the construction time and overall construction cost. Mechanically fastened shear connectors represent a viable alternative to headed studs, considering their fast installation process and shear resistance. X-HVB shear connectors are attached to the steel beam with two cartridge fired pins. The first step towards extensive implementation of X-HVB shear connectors in composite construction is to understand their behaviour through experimental investigation. Results of the push-out tests, in accordance to Eurocode 4, with X-HVB 110 shear connectors positioned in envisaged openings of prefabricated concrete slabs are presented in this paper. The experimental investigation comprised three different specimen's layout. Group arrangement of X-HVB shear connectors in envisaged openings included specimens with minimal recommended distances and specimens with reduced distances between connectors in both directions. Influence of different installation procedures on overall behaviour of the connection is presented, as well as the orientation of shear connectors relative to the shear force direction. Influence of variations is characterized in terms of failure mechanisms, shear resistance and ductility.

• Journal of Periodontal and Implant Science
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• 제25권1호
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• pp.1-13
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• 1995

Chlorhexidine and Listerine are widely used in dentistry due to its effectiveness on plaque control and bactericidal action. The effects of these agent on chronic gingivitis and wound healing following surgical periodontal therapy in human has been favorable. Understanding the effects of chlorhexidine and Listerine on human gingival fibroblast will provide the rationale for its use during the healing process of periodontal surgery. The purpose of this study was to compare the effects of chlorhexidine and Listerine on human gingival fibroblast. Human gingival fibroblasts were cultured from the healthy gingiva on the extracted premolar of orthodontic patients. Human gingival fibroblast were trypsinized and cultured in growth medium added range of 0.0012-0.12% chlorhexidine and 1-100% Listerine mouth wash solution. The cell used in this study were between fifth to eighth passage number. The cell morphology were examined by inverted microscope and the cell activity were measured by MIT assay. The Morphology of gingival fibroblast added Chlorhexidine and Listerine at the concentration of all range were became globular and lost their cytoplasmic process. Our results indicate that a 0.0012 concentration of chlorhexidine and 1% concentration of Listerine were shows minimal cytotoxicity, but above these concentraion, there was a significant difference between the cell activity in the experimental group and control group(p