Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Antibacterial effect of bee venom against Gram-positive and negative bacteria isolated from mastitis in dairy cattle

봉독의 젖소 유방염 유래 그람 양성 및 음성 세균별 항균효과 분석

  • Jung, Sukhan (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Sang-Ik (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Han-Gyu (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Young-Hun (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-Young (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration) ;
  • Han, Sangmi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Baek, Kui-Jeong (Jeollabuk-do Institute of Livestock & Veterinary Research) ;
  • Cho, Ara (Division of Animal Diseases and Health, National Institute of Animal Science, Rural Development Administration)
  • 정숙한 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 오상익 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 이한규 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 정영훈 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 허태영 (농촌진흥청 국립축산과학원 가축질병방역과) ;
  • 한상미 (농촌진흥청 국립농업과학원 잠사양봉소재과) ;
  • 백귀정 (전라북도 동물위생시험소) ;
  • 조아라 (농촌진흥청 국립축산과학원 가축질병방역과)
  • Received : 2021.09.19
  • Accepted : 2021.09.29
  • Published : 2021.09.30
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Abstract

Mastitis is an inflammatory condition of the mammary gland, most often caused by bacterial infections, resulting in significant economic losses to the dairy industry. Antimicrobial resistance has been of great concern because of the extensive clinical use of antibiotics. For this reason, the development of new compounds as an alternative treatment to bovine mastitis is needed. Bee venom has been widely used as an oriental treatment for several inflammatory diseases and bacterial infections. The aim of the present study was to evaluate the antimicrobial activity of bee venom on bacteria isolated from bovine mastitis. A total of 107 isolates from bovine mastitic milk samples collected in 2019 and 2020 in Jeonbuk province. All bacterial isolates were tested for susceptibility to bee venom of the honey bee (Apis mellifera). In order to obtain comprehensive antibacterial activities of the bee venom, we measured the minimal inhibitory concentration (MIC) of the bee venom against bacterial strains. Bee venom showed significant inhibition of bacterial growth of Gram-negative bacteria Citrobacter spp., Escherchia coli, Klebsiella spp., Pseudomonas spp., Serratia spp. and Raoultella with MIC values of 96, 81, 72, 230, and 85 ㎍/mL, respectively, and Gram-positive bacterial Enterococcus spp., Staphylococcus spp. and Streptococcus spp. with MIC values of 29, 21 and 16 ㎍/mL, respectively. The results indicated that the MIC values were different depending on the bacterial strains, and those of Gram-positive bacteria were lower than those of Gram-negative bacteria for bee venom. These findings suggested that bee venom could be an effective antimicrobial treatment for bovine mastitis; however, further research is necessary to evaluate the mechanism underlying the antimicrobial action, its effectiveness/safety in vivo and effective application for therapeutic use.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(세부과제명: 가축 유래 세포주에서 봉독의 안전성 및 작용 효과 분석, 세부과제번호: PJ014298022021)의 지원 및 2021년도 농촌진흥청(국립축산과학원) 전문연구원 과정 지원사업에 의해 이루어진 것임.

References

  1. 남향미. 2010. 젖소 유방염에 대한 항생제 사용 지침. 대한수의사회지. 46(7): 638-644.
  2. Eberhart RJ, Natzke RP, Newbould FSH. 1979. Coliform mastitis-a review. J Dairy Sci 62(1): 1-22. https://doi.org/10.3168/jds.S0022-0302(79)83196-3
  3. Erskine RJ, Tyler TW, Riddler MG. 1991. Theory use and realities of efficacy and food safety of antimicrobial treatment of acute coliform mastitis. J Am Vet Med Assoc 198(6): 980-984.
  4. Habermann, E. and Reiz, K. G. 1965. On the biochemistry of bee venom peptides, melittin and apamine. Biochemistry 343(2): 192-203.
  5. Han SM, Hong IP, Woo SO, Kim SG, Jang HR. 2015. Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed preprocessing method. Korean J Vet Serv 38(1): 25-30. https://doi.org/10.7853/kjvs.2015.38.1.25
  6. Han SM, Kim JM, Yeo JH, Hong IP, Woo SO, Lee KG, Kweon HY. 2014. Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients. Korean J Vet Serv 37(2): 123-129. https://doi.org/10.7853/kjvs.2014.37.2.123
  7. Han SM, Kim SG. Hong IP, Woo SO, Jang HR, Lee KW. 2016. Antibacterial effects of purified bee venom against some pathogenic bacteria isolated from dead chickens. Korean J Vet Serv 39(3): 159-166. https://doi.org/10.7853/kjvs.2016.39.3.159
  8. Han SM, Lee KG, Yeo JH, Hwang SJ, Jang CH, Chenoweth PJ, Pak SC. 2009. Effects of bee venom treatment on growth performance of young pigs. Am J Chin Med 37(2): 253-260. https://doi.org/10.1142/S0192415X09006813
  9. Han SM, Lee KG, Yeo JH, Kweon HY, Kim BS, Kim JM, Baek HJ, Kim ST. 2007. Antibacterial activity of the honey bee venom aganist bacterial mastitis pathogens infecting dairy cows. Int J Indust Entomol 14(2): 137-142.
  10. Han SM, Lee KG, Yeo JH, Kweon HY, Woo SO, Oh BY, Lee YG, Kim BS, Baek HJ, Kim ST. 2007. Therapeutic effects of honeybee (Apis Mellifera L.) venom injection on bovine mastitis. Korean J Vet Serv 30(1): 115-123.
  11. Han SM, Woo SO, Kim SG, Jang HR, Lee KW. 2018. Antibacterial effects of purified bee venom against Bacillus cereus, Streptococcus agalactiae, and Pseudomonas aeruginosa. Journal of Apiculture 33(1): 9-16. https://doi.org/10.17519/apiculture.2018.04.33.1.9
  12. Jeong CH, Cheng WN, Bae H, Lee KW, Han SM, Petriello M C, Lee HG, Seo HG, Han SG. 2017. Bee venom decreases LPS-induced inflammatory responses in bovine mammary epithelial cells. J Microbiol Biotechnol 27(10): 1827-1836. https://doi.org/10.4014/jmb.1706.06003
  13. Kang HJ, Kim IC, Kim JH, Son WG, Lee DS. 2001 Identification and antimicrobial susceptibility of microorganisms isolated from bovine mastitic milk. Korean J Vet Serv 41(4): 511-521.
  14. Kim DI, Kim EK, Seong WJ, Ro YH, Ko DS, Kim NH, Kim JH, Kwon HJ. 2017. Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis. Korean J Vet Res 57(2): 117-126. https://doi.org/10.14405/kjvr.2017.57.2.117
  15. Kim J, Ochoa, MT, Krutzik, SR, Takeuchi, O, Uematsu S, Legaspi AJ, Brightbill HD, Holland D, Cunliffe WJ, Akira S, Sieling PA, Godowski PJ, Modlin RL. 2002. Activation of toll-like receptor 2 in acne triggers inflammatory cytokine responses. J. Immunol 169(3):1535-41. https://doi.org/10.4049/jimmunol.169.3.1535
  16. Kim JH, Kim BM, Ham JS, Oh MH. 2017. Detection and Characteristics of Coagulase-Negative Staphylococcus sp. isolated from Dairy Cattle Milk. J Milk Sci Biotechnol 35(3): 162-168. https://doi.org/10.22424/jmsb.2017.35.3.162
  17. Kim SE, Hah DY, Jang EH, Kwon HN, Jo SS, Kwon YT, Park DY, Lee KC, Kim JS. 2011. Survey of mastitis management and incidence of mastitis in high somatic cell count of bulk milk at dairy farms in the Gyeongnam. Korean J Vet Serv 34(4): 379-388. https://doi.org/10.7853/kjvs.2011.34.4.379
  18. Kim ST, Hwang JY, Sung MS, Je SY, Bae DR, Han SM, Lee SH. 2006. The minimum inhibitory concentration (MIC) of bee venom against bacteria isolated from pigs and chickens. Korean J Vet Serv 29(1):19-26.
  19. Kim ST, Kim S, Kim SY, Son JK. 1997. Comparison of fatty acid composition of Staphylococcus sp. isolated from bovine mastitis milk. Korean J Vet Serv 20(1): 37-45.
  20. Lee DG, Shin HH. 2008. Pharmacokinetics and pharmacodynamics of antibiotics: General concepts and recent advances. Infect Chemother 40(3): 140-147. https://doi.org/10.3947/ic.2008.40.3.140
  21. Lee ES, Kang HM, Chung CI, Moon JS. 2007. Antimicrobial susceptibility and prevalence of gram-negative bacteria isolated from bovine mastitis. Korean J Vet Res 47(4): 67-75.
  22. Lee G, Kang HM, Chung CI, Moon JS. 2007. Antimicrobial susceptibility and genetic characteristics of Streptococcus uberis isolated from bovine mastitis milk. Korean J Vet Res 47(1): 33-41.
  23. Lee SJ, Kim KH, Lee WR, Kim JY, An HJ, Park KK. 2015. Anti-bacterial and anti-inflammatory effect of melittin on propionibacterium acnes-induced inflammatory skin disease in vivo. Journal of Apiculture 30(2): 95-101. https://doi.org/10.17519/apiculture.2015.06.30.2.95
  24. Leitner Gabriel, Lubashevsky, Evgenia, Glickman Anita, Winkler Marta, Saran Arthur, Trainin Zeev. 2003. Development of a Staphylococcus aureus vaccine against mastitis in dairy cows. Vet Immunol. Immunopathol 93(1-2): 31-38. https://doi.org/10.1016/S0165-2427(03)00051-5
  25. Nakamura S, Kato AM, Kobayashi K. 1991. New antimicrobial characteristics of lysozyme-dextran conjugate. J Agric Food Chem 39:647-650. https://doi.org/10.1021/jf00004a003
  26. Oh BY, Han SM, Oh YI, Kim ST. 2011. Effects of the blood chemistry of honeybee (Apis mellifera L.) venom on the Hanwoo calves. Korean J Vet Serv 34(1): 87-93. https://doi.org/10.7853/kjvs.2011.34.1.087
  27. Park JK, Shen CZ, Kim CG, Kim IK. 2016. The stability comparison of purified bee venom and bee venom melittin in aqueous solution. Anal. Sci. Technol 29(4): 194-201. https://doi.org/10.5806/AST.2016.29.4.194
  28. Park YJ, Yang DK, Han HY. 2011. Efficacy of a herd specific mastitis vaccine against Staphylococcus aureus in dairy cows. Journal of preventive veterinary medicine 35(2): 81-89.
  29. Rudenko SV, Nipot EE. 1996. Modulation of melittin-induced hemolysis of erythrocytes. Biokhimiia 61(12), 2116-2124.
  30. Steiner, H., D. Hultmark, A. Engstrom, H. Bennich and H.G. Boman. 1981. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 292(5820): 246-248. https://doi.org/10.1038/292246a0
  31. Watts, J. 1988. Etiological agents of bovine mastitis. Vet Microbiol 16(1): 41-66. https://doi.org/10.1016/0378-1135(88)90126-5
  32. Zhao X, Lacasse P. 2008. Mammary tissue damage during bovine mastitis: causes and control. J Anim Sci 86(13): 57-65. https://doi.org/10.2527/jas.2007-0302