• Bulletin of the Korean Chemical Society
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• v.22 no.4
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• pp.337-348
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• 2001

This article gives an overview of a recently developed channel system, frit-inlet asymmetrical flow field-flow fractionation (FI-AFlFFF), which can be applied for the separation of nanoparticles, proteins, and water soluble polymers. A conventiona l asymmetrical flow FFF channel has been modified into a frit-inlet asymmetrical type by introducing a small inlet frit near the injection point and the system operation of the FI-AFlFFF channel can be made with a great convenience. Since sample components injected into the FI-AFlFFF channel are hydrodynamically relaxed, sample injection and separation processes proceed without interruption of the migration flow. Therefore in FI-AFlFFF, there is no requirement for a valve operation to switch the direction of the migration flow that is normally achieved during the focusing/relaxation process in a conventional asymmetrical channel. In this report, principles of the hydrodynamic relaxation in FI-AFlFFF channel are described with equations to predict the retention time and to calculate the complicated flow variations in the developed channel. The retention and resolving power of FI-AFlFFF system are demonstrated with standard nanospheres and protreins. An attempt to elucidate the capability of FI-AFlFFF system for the separation and size characterization of nanoparticles is made with a fumed silica particle sample. In FI-AFlFFF, field programming can be easily applied to improve separation speed and resolution for a highly retaining component (very large MW) by using flow circulation method. Programmed FI-AFlFFF separations are demonstrated with polystyrene sulfonate standards and pululans and the dynamic separation range of molecular weight is successfully expanded.

• Journal of Contemporary Eastern Asia
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• v.19 no.2
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• pp.84-103
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• 2020

Spurred by the ongoing globalization, an increase in mobility has diversified migrant categories and strengthened intercultural rapport. Alongside the "traditional" migrants, "White" (Caucasian) individuals are coming into greater focus of migration studies as "lifestyle migrants". Although White migrations are not a new phenomenon, the deep-seated idea of White supremacy continues to play an important role in contemporary intercultural communication, awarding Whites across communities a "cosmopolitan" status of highly educated cultural elites. As such, the focus of this research is on highly skilled White European migrants, on their subjective experiences of integration in Japan, and whether they perceive Whiteness as an obstacle or an advantage in this process, if integration is desired at all. To discern the connection between race and integration, this research investigates the non-White majority society of Japan as it has established racial hierarchies according to the Western models, consequently influencing the status of its contemporary White immigrants. Privileged, yet singled out as racial and cultural role models, White Europeans' integration seemingly becomes nearly impossible. The data obtained in fifteen semi-structured interviews confirms that Whiteness grants advantages when entering the Japanese job market, but remains an obstacle in everyday community integration. European professionals do not feel accepted and abandon efforts to integrate, if such were made, retreating into "cosmopolitan islets" wherein they renegotiate their White European identities.

• BMB Reports
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• v.55 no.5
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• pp.220-225
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• 2022

Hepatocellular carcinoma (HCC), a primary type of liver cancer, is one of the leading causes of cancer related deaths worldwide. HCC patients have poor prognosis due to intrahepatic and extrahepatic metastasis. Hepatitis B virus (HBV) infection is one of the major causes of various liver diseases including HCC. Among HBV gene products, HBV X protein (HBx) plays an important role in the development and metastasis of HCC. However, the mechanism of HCC metastasis induced by HBx has not been elucidated yet. In this study, for the first time, we report that HBx interacts with the suppressor of cytokine signaling 1 (SOCS1) which negatively controls NF-κB by degrading p65, a subunit of NF-κB. NF-κB activates the transcription of factors associated with epithelial-mesenchymal transition (EMT), a crucial cellular process associated with invasiveness and migration of cancer cells. Here, we report that HBx physically binds to SOCS1, subsequently prevents the ubiquitination of p65, activates the transcription of EMT transcription factors and enhance cell migration and invasiveness, suggesting a new mechanism of HBV-associated HCC metastasis.

• Natural Product Sciences
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• v.28 no.2
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• pp.80-88
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• 2022

Colorectal cancer is one of the most common cancers globally, ranking second for the number of cancer-related deaths. Metastasis has been reported as the main cause of death in patients with colorectal cancer. Peroxisome proliferator-activated receptor gamma (PPAR-γ) is a transcription factor that functions as a tumor suppressor by inhibiting cellular proliferation, migration, and invasion. In our previous efforts to generate natural product-motivated PPAR-γ ligands, the compounds 1 and 2 were obtained. These compounds activated PPAR-γ and inhibited the migration and invasion of HCT116 colorectal cancer cells, and they were also found to inhibit the epithelial-to-mesenchymal transition, which is a key process in cancer metastasis. Compounds 1 and 2 upregulated expression of the epithelial marker (E-cadherin), and downregulated expression of the mesenchymal marker (N-cadherin) and transcriptional factor (Snail). Therefore, the PPAR-γ agonists 1 and 2 could serve as a valuable model for the study on anti-metastatic leads for the treatment of colorectal cancer.

• Journal of Integrative Natural Science
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• v.16 no.2
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• pp.47-51
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• 2023

Ras small GTPases are involved in regulating various cellular signaling pathways including cell migration, proliferation, and differentiation. Ras GTPase subfamily is comprised of 15 proteins; 11 Ras, 3 Rap, and one Rheb related protein. Some Ras proteins, such as RasC and RasG, have been identified for their major functions, but there are proteins whose functions have not been studied yet, such as RasU and RasX. Here, we investigated the roles of RasU in cell motility and development. RasU shows the highest homology with RasX. To investigate the functions of RasU, rasU null cells were used to observe the phenotype. Cells lacking RasU were larger and more spread than wild-type cells. These results indicate that RasU plays a negative role in cell spreading. In addition, we investigated the roles of RasU in cell motility and development of Dictyostelium cells and found that rasU null cells exhibited decreased random migration speed and delayed developmental process. These results suggest that RasU plays an important role in cell motility and development.

• Food Science of Animal Resources
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• v.44 no.6
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• pp.1266-1282
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• 2024

To improve the antimicrobial and antioxidant characteristics of chitosan (CH), a conjugate of gallic acid (GA) and chitosan (GA-g-CH) was synthesized through a radical grafting process. The impact of the addition of GA-g-CH on the quality of chicken patties was investigated during a 15-day period under refrigerated conditions. The microbiological characteristics, encompassing the total viable counts, counts of Pseudomonas spp., and counts of lactic acid bacteria were assessed. Furthermore, the water migration, sensory characteristics, and physicochemical characteristics, including thiobarbituric acid-reactive substances (TBARS), carbonyl content, pH level, water holding capacity (WHC), and color deterioration were also evaluated. The findings suggest that both CH+GA and GA-g-CH addition effectively maintained the quality of chicken patties during cold storage. Nevertheless, GA-g-CH exhibited superior antimicrobial properties and a stronger capacity to inhibit the formation of TBARS and carbonyl compounds. The addition of GA-g-CH also inhibited water migration, maintained a higher WHC, and resulted in superior sensory attributes for a longer duration compared to the other treated samples, thus prolonging the shelf life and retarding the deterioration of fresh chicken patties by 3-6 days during refrigerated storage. The research findings suggest that the incorporation of GA-g-CH exhibits promising potential in maintaining the freshness of ground chicken products during storage.

• Journal of the Korean Home Economics Association
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• v.45 no.1
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• pp.9-21
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• 2007

This research investigated the retired elderly who had moved to rural areas. The propose of the study was to examine the point at which there must be a difference in the patterns of a return to farming and determine the actual condition of their preparations for migration. This study had surveyed 408 seniors who wereare over 50 years old and had moved to rural areas after their retirement and analyzed the data by the SPSS PC 11.0 program. The results were as follows. First, the relevancy of U-turn, J-turn, and I-turn types that were affected by social demography was found to be dependent on their education levels and family patterns. Second, the actual conditions of the process of preparation by the types of a return to the farming were different according to the motivation and preparation fund. Nevertheless, the most important factor was the influence of their spouses. Third, the actual conditions of the process of adaptation by the patterns of a return to the farming showed no difference between the degree of efforts of the social supportand elevation of the friendship among the neighbors. The recognition of rural life problems were more acquainted towards the area of farming life. Among the 6 problem areas, leisure, health care, and economic problems were highly considered.

• Journal of Soil and Groundwater Environment
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• v.9 no.3
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• pp.20-26
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• 2004

In-situ photolysis is one of the most promising ways to clean up a soil contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). This study focuses on the mathematical description and model development of the convective upward transport of an organic solvent driven by evaporation and photodecomposition at the surface as the major transport mechanism in the clean up process. A finite-element-based numerical model was proposed to incorporate effects of multiphase flow on the distribution of each fluid, gravity as a driving force, and the use of van Genutchen equation for more accurate description of k-S-p relations. This paper presents results of extensive numerical calculations conducted to investigate the various parameters that play a role in the solvent migration through a laboratory-scale unsaturated soil column. The numerical results indicate that gravity affects significantly on the fluids distribution and evaporation for highly permeable soils. The soil texture has a profound influence on the fluid saturation profile during evaporation process. The amount of solvent convective motion increases with increasing evaporation rates and decreasing initial water saturation. Simulations conducted in this study have shown that the developed model is very useful in analyzing the effects of various parameters on the convective migration of an organic solvent in the soil environments.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.05a
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• pp.280-283
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• 2014

Cloud Computing virtualizes logical resources like cpu, memory and disks etcs from physical machines. This virtualization technology increases computing resource utilization and supports dynamic resource allocations. Because of these benefits, global cloud computing services like Amazon AWS, Google Cloud and Apple iCloud are prevalent. With this cloud Computing services, there has been a request about cloud migration between different cloud environments. If one service which operates in a cloud computing environment wants to migrate to another cloud environment, there should have been a compitability between two different cloud environment. but even global cloud computing services do not support this compitability. In this paper, we suggest a process and technology to cloud migrations based on open sources.

• Economic and Environmental Geology
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• v.51 no.3
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• pp.223-232
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• 2018

The viscous force of fluids and the capillary force acting on the pore network of the porous media are important factors determining the immiscible displacement during geological $CO_2$ sequestration, these were directly affected by geological formation conditions and injection conditions. This study aimed to observe the migration and distribution of injected fluid and pore water, and quantitatively investigate displacement efficiency on various injection temperatures. This study aimed to perform micromodel experiments by applying n-hexane used as a proxy fluid for supercritical $CO_2$. In this study, immiscible displacement process from beginning of n-hexane injection to equilibrium of the distribution of the n-hexane and pore water was observed. The images from experiment were used to observe the displacement pattern and estimate the areal displacment efficiency of the n-hexane. For investigate the affects of the injection temperatures on the migration in macroscopic, migration of n-hexane in single pore was analyzed. The measurement revealed that the displacement efficiency at equilibrium state decreases as the temperature increases. The result from experiments indicate that the temperatures can affect the displacement pattern by changing the viscous forces and the capillary forces. The experimental results could provide important fundamental information on reservoir conditions and fluid injection conditions during geological $CO_2$ sequestration.