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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Hepatitis B virus X protein promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells by regulating SOCS1

  • Kang, Inho (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Ji Ae (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Jinchul (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Lee, Ju Hyeon (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Mi-jee (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Ahn, Jeong Keun (Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • Received : 2021.11.01
  • Accepted : 2021.12.26
  • Published : 2022.05.31
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Abstract

Hepatocellular carcinoma (HCC), a primary type of liver cancer, is one of the leading causes of cancer related deaths worldwide. HCC patients have poor prognosis due to intrahepatic and extrahepatic metastasis. Hepatitis B virus (HBV) infection is one of the major causes of various liver diseases including HCC. Among HBV gene products, HBV X protein (HBx) plays an important role in the development and metastasis of HCC. However, the mechanism of HCC metastasis induced by HBx has not been elucidated yet. In this study, for the first time, we report that HBx interacts with the suppressor of cytokine signaling 1 (SOCS1) which negatively controls NF-κB by degrading p65, a subunit of NF-κB. NF-κB activates the transcription of factors associated with epithelial-mesenchymal transition (EMT), a crucial cellular process associated with invasiveness and migration of cancer cells. Here, we report that HBx physically binds to SOCS1, subsequently prevents the ubiquitination of p65, activates the transcription of EMT transcription factors and enhance cell migration and invasiveness, suggesting a new mechanism of HBV-associated HCC metastasis.

Keywords

Acknowledgement

This research was supported by the research fund of Chungnam National University.

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