• Title/Summary/Keyword: microscopic characteristics

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국내 식물시료에서 분리한 Bacillus thuringiensis 균주의 다양성

  • Park, Seung-Hwan;Koo, Bon-Tag;Shin, Byung-Sik;Choi, Soo-Keun;Jeong, Young-Mee;Pan, Jae-Gu;Kim, Jeong-Il
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.159-165
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    • 1997
  • We collected 3,237 plant samples, mainly leaves of various trees, from many provinces in Korea and a total of 1,925 Bacillus thuringiensis isolates were obtained and characterized. The isolates were characterized in terms of crystal morphology, PAGE pattern of the toxin proteins, plasmids pattern, biochemical characteristics, and bioassay. The microscopic observation showed that 49.1% of the isolates have bipyramidal shape crystals, 7.1% of spherical shape crystals, 1.4% of rhomboidal shape crystals, and others have small or amorphous inclusions. The insecticidal activities of the spore-crystal mixtures of isolates were tested against Plutella xylostella, Bombyx mori, Culex pipiens, and Agelastica coerulea. Bioassay showed that 51.3% of the isolates were shown to be active; lepidopteran-specific (44.8%), dipteran-specific(4.9%) and coleopteran-specific (1.6%). The remainder(48.8%) did not show any activity against the insects we tested. Interestingly though, some of these non-active isolates were shown to have bipyramidal crystals. By serotyping 22 isolates of our collection, we found that there are various kind of subspecies such as aizawai, amagiens, canadensis, darmstadiensis, galleriae, finitimus, kurstaki, morrisoni and neoleonensis, and three isolates have been classified into a new serotype, H49, and one of them, the type strain, named subsp. muju. From this study it was found that phylloplane is a good source for the isolation of Bacillius thuringiensis, and Bacillus thuringiensis is distributed widely in Korea.

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A Study for the Weldability and Welded Joint Performance on the SAW of HSA800 Steel (HSA800 강재의 SAW 용접성 및 이음성능 연구)

  • Choi, Young Han;Kim, Sang Seup
    • Journal of Korean Society of Steel Construction
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    • v.29 no.1
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    • pp.37-48
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    • 2017
  • In this research, 9 SAW welding specimens with 1 hour post-heat treatment (PH1) and 2 hours (PH2) were experimented to confirm the weldability and the joint performance of 60mm thickness HSA800 steel. SAW tests with PH1 & PH2, including weld impact test, bend test, hardness test, macroscopic test, and microscopic test, had a good test results. However, from the chemical composition test results, P composition was not satisfied with Korean Industrial Standards(KS). According to the weld-zone tensile tests, 2 hours post-heat SAW is recommended because the mechanical characteristics of SAW PH2 were better than those of SAW PH1.

Single crystals growth and properties of $LiNbO_{3}$ doped with MgO or ZnO : (I) Single crystals growth and their defect structure (MgO 또는 ZnO를 첨가한 $LiNbO_{3}$ 단결정 성장 및 특성 : (I) 단결정 성장 및 결함구조)

  • Cho, Hyun;Shim, Kwang-Bo;Auh, Keun-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.368-376
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    • 1996
  • $LiNbO_{3}$ single crystals (undoped, 5 mol% MgO-doped and 5 mol% ZnO-doped) were grown by the floating zone method which has the characteristics of a compositional homogeneity and uniform distribution of the dopants. The optimum growth condition was established experimentally and the defect structures such as domain structure, dislocation structure, slip band, and microtwins were characterized using a microscopic method.

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Effects of Hybrid Coat on shear bond strength of five cements: an in-vitro study

  • Guo, Yue;Zhou, Hou-De;Feng, Yun-Zhi
    • The Journal of Advanced Prosthodontics
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    • v.9 no.6
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    • pp.447-452
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    • 2017
  • PURPOSE. To evaluate the sealing performance of Hybrid Coat and its influence on the shear bond strength of five dentin surface cements. MATERIALS AND METHODS. Six premolars were pretreated to expose the dentin surface prior to the application of Hybrid Coat. The microscopic characteristics of the dentinal surfaces were examined with scanning electron microscopy (SEM). Then, 40 premolars were sectioned longitudinally, and 80 semi-sections were divided into a control group (untreated) and a study group (treated by Hybrid Coat). Alloy restoration was bonded to the teeth specimen using five different cements. Shear bond strength was measured by the universal testing machine. The fracture patterns and the adhesive interface were observed using a stereomicroscope. RESULTS. SEM revealed that the lumens of dentinal tubules were completely occluded by Hybrid Coat. The Hybrid Coat significantly improved the shear bond strength of resin-modified glass ionomer cement (RMGIC) and resin cement (RC) but weakened the performance of zinc phosphate cement (ZPC), zinc polycarboxylate cement (ZPCC) and glass ionomer cement (GIC). CONCLUSION. Hybrid Coat is an effective dentinal tubule sealant, and therefore its combined use with resin or resin-modified glass ionomer cements can be applied for the prostheses attachment purpose.

Analysis of Wear Debris for Machine Condition Diagnosis of the Lubricated Moving Surface (기계윤활 운동면의 작동상태 진단을 위한 마멸분 해석)

  • Seo, Yeong-Baek;Park, Heung-Sik;Jeon, Tae-Ok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.5
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    • pp.835-841
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    • 1997
  • Microscopic examination of the morphology of wear debris is an accepted method for machine condition and fault diagnosis. However wear particle analysis has not been widely accepted in industry because it is dependent on expert interpretation of particle morphology and subjective assessment criteria. This paper was undertaken to analyze the morphology of wear debris for machine condition diagnosis of the lubricated moving surfaces by image processing and analysis. The lubricating wear test was performed under different sliding conditions using a wear test device made in our laboratory and wear testing specimen of the pin-on-disk-type was rubbed in paraffine series base oil. In order to describe characteristics of debris of various shape and size, four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) have been developed and outlined in the paper. A system using such techniques promises to obviate the need for subjective, human interpretation of particle morphology in machine condition monitoring, thus to overcome many of the difficulties in current methods and to facilitate wider use of wear particle analysis in machine condition monitoring.

Wear Behavior of TiN Coatings Deposited on High Speed Steel and Alloy Tool Steel (TiN 코팅된 고속도강과 합금공구강의 마멸거동)

  • 김석삼;서창민;박준목
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.3
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    • pp.705-712
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    • 1995
  • The wear characteristics and wear mechanisms in TiN coating deposited on high speed steel and alloy tool steel by ion plating were investigated. Pin on V-block wear tester was used for a wear test method. The specimen was composed of three kinds of high speed steel and alloy tool steel which had different hardness by changing the heat treating condition. Three kinds of coating thickness were also applied to each specimen. Microscopic observation of worn surfaces was made by SEM. The scratch test of coating surface by the ion plating showed that critical load to break the coating interface was greater than 50N. The critical load increased with both substrate hardness and coating thickness. The wear resistance of TiN coated high speed steel became 10 times greater than that of non-coated ones. SEM observation showed that leading edge of contact was compressive and trailing edge was under maximum tensile stress and then surface cracking broke out perpendicular to sliding direction.

Histological and Cytological Changes Associated with Susceptible and Resistant Responses of Chili Pepper Root and Stem to Phytophthora capsici Infection

  • Kim, Sang-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.25 no.2
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    • pp.113-120
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    • 2009
  • Microscopic study of chili pepper (Capsicum annuum L.) infected with Phytophthora capsici, causing Phytophthora blight of chili pepper, was conducted to compare histological and cytological characteristics in the root and stem of susceptible (C. annuum cv. Bugang) and resistant (C. annuum cv. CM334) pepper cultivars. The susceptible pepper roots and stems were extensively penetrated and invaded by the pathogen initially into epidermal cells and later cortical and vascular cells. Host cell walls adjacent to and invaded by the infecting hyphae were partially dissolved and structurally loosened with fine fibrillar materials probably by cell wall-degrading enzymes of the pathogen. In the resistant pepper, the pathogen remained on root epidermal surface at one day after inoculation, embedded and captured in root exudation materials composed of proteins and polysaccharides. Also the pathogen appeared to be blocked in its progression at the early infection stages by thickened middle lamellae. At 3 days after inoculation, the oomycete hyphae were still confined to epidermal cells of the root and at most outer peripheral cortical cells of the stem, resulting from their invasion blocked by wound periderms formed underneath the infection sites and/or cell wall appositions bounding the hyphal protrusions. All of these aspects suggest that limitation of disease development in the resistant pepper may be due to the inhibition of the pathogen penetration, infection, invasion, and colonization by the defense structures such as root exudation materials, thickened middle lamellae, wound peridems and cell wall appositions.

School Violence Experience and Coping of Students with High Functioning Autism Spectrum Disorders in Inclusive Education Environment (통합교육 현장에서 고기능의 자폐스펙트럼장애 학생의 학교폭력 경험과 대처)

  • Lee, Kowoon;Jung, Suna
    • Journal of The Korean Society of Integrative Medicine
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    • v.4 no.3
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    • pp.69-79
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    • 2016
  • PURPOSE : The purpose of the this study was to build an understanding of school violence experiences among students with high functioning autism spectrum disorders(HF-ASD) in inclusive education environment and provide basic data to prevent and intervene it. METHOD : Sixteen mothers having a son with HF-ASD were interviewed in-depth and shared their son's experiences. The qualitative research method are used in the collection and analysis of data. RESULT : As a result of this research, 4 categories, 11 sub-categories and 80 concepts are identified. 4 categories are as follows: characteristics of school violence experience, results of school violence experience, the context of school violence occurs, and coping to school violence. CONCLUSION : The result of this study should have a microscopic viewpoint of their suffering and help to identify the needs for school violence prevention and support. It is also to be utilized as a successful coping strategies for HF-ASD and their parent education.

Isolation and Identification of Actinomycetes F-97 Producing Tyrosinase Inhibitor (Tyrosinase 저해제를 생성하는 방선균 F-97의 분리 및 동정)

  • Bang, Byung-Ho;Rhee, Moon-Soo;Kim, Gwan-Pil;Kim, Jin-O;Yi, Dong-Heui
    • The Korean Journal of Food And Nutrition
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    • v.22 no.1
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    • pp.97-102
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    • 2009
  • In order to obtain a non-toxic and more active and stable microorganism-produced tyrosinase inhibitor, we isolated actinomycetes F-97, a producer of tyrosinase inhibitor, from soil. The aerial hyphae of this strain were gray in color with tree types. Under the microscopic examination, the isolate formed a spiral aerial spore mass with a smooth surface. The analysis of cell wall acid hydrolysate of the isolate revealed the presence of LL-diaminopimelic acid(LL-DAP). No specific sugar was detected. From these results and the cultural and physiological characteristics described in the Bergey's Manual, actinomycetes F-97 was identificated as, or best-matched to, Streptomyces aburaviensis.

A multiscale method for analysis of heterogeneous thin slabs with irreducible three dimensional microstructures

  • Wang, Dongdong;Fang, Lingming
    • Interaction and multiscale mechanics
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    • v.3 no.3
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    • pp.213-234
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    • 2010
  • A multiscale method is presented for analysis of thin slab structures in which the microstructures can not be reduced to two-dimensional plane stress models and thus three dimensional treatment of microstructures is necessary. This method is based on the classical asymptotic expansion multiscale approach but with consideration of the special geometric characteristics of the slab structures. This is achieved via a special form of multiscale asymptotic expansion of displacement field. The expanded three dimensional displacement field only exhibits in-plane periodicity and the thickness dimension is in the global scale. Consequently by employing the multiscale asymptotic expansion approach the global macroscopic structural problem and the local microscopic unit cell problem are rationally set up. It is noted that the unit cell is subjected to the in-plane periodic boundary conditions as well as the traction free conditions on the out of plane surfaces of the unit cell. The variational formulation and finite element implementation of the unit cell problem are discussed in details. Thereafter the in-plane material response is systematically characterized via homogenization analysis of the proposed special unit cell problem for different microstructures and the reasoning of the present method is justified. Moreover the present multiscale analysis procedure is illustrated through a plane stress beam example.