The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Isolation and Identification of Actinomycetes F-97 Producing Tyrosinase Inhibitor

Tyrosinase 저해제를 생성하는 방선균 F-97의 분리 및 동정

  • Published : 2009.03.31
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Abstract

In order to obtain a non-toxic and more active and stable microorganism-produced tyrosinase inhibitor, we isolated actinomycetes F-97, a producer of tyrosinase inhibitor, from soil. The aerial hyphae of this strain were gray in color with tree types. Under the microscopic examination, the isolate formed a spiral aerial spore mass with a smooth surface. The analysis of cell wall acid hydrolysate of the isolate revealed the presence of LL-diaminopimelic acid(LL-DAP). No specific sugar was detected. From these results and the cultural and physiological characteristics described in the Bergey's Manual, actinomycetes F-97 was identificated as, or best-matched to, Streptomyces aburaviensis.

본 연구에서는 독성이 적고, 활성이 높으면서 안정한 새로운 tyrosinase 저해제를 미생물을 대상으로 얻고자 tyrosinase 저해제를 강하게 분비하는 방선균 F-97을 토양으로부터 분리하였다. 이 균의 기균사는 회색의 나뭇가지형 (tree type)이며, 포자의 끝부분이 유선형으로 회전되어 있고, 포자 표면은 구형의 smooth형으로 분류되었다. 세포벽의 DAP 성분은 LL-DAP이었으며, 세포균체의 당 성분은 검출되지 않았다. 기타 배양학적 및 생리적 특성을 종합한 결과 streptomyces aburaviensis 또는 근연균으로 추정된다.

Keywords

References

  1. Lee, HJ, Lee, MK and Park, IS. Characterization of mushroom tyrosinase inhibitor in sweet potato. J. Life Sci. 16:396-399. 2006 https://doi.org/10.5352/JLS.2006.16.3.396
  2. Jung, SW, Lee, NK, Kim, SJ and Han, DS. Screening of tyrosinase inhibitor from plants. Kor. J Food Sci. Tech. 27:891-896. 1995
  3. Yoneta, AT, Yamashita, HY, Jin, S, Kondo and Jimbow, K. Ectopic expression of tyrosinase increases melanin synthesis and cell death following UVB irradiation in fibroblasts from familial atypical multiple mole and melanoma(FAMMM) patients. Melanoma Res. 14:387-394. 2004 https://doi.org/10.1097/00008390-200410000-00009
  4. Park, SS, Ryu, YB, Lee, YH, Cho, YU, Cho, SJ, Choi, YJ, Park, KH and Gal, SW. Inhibition of melanin synthesis by mycelial culture broth of Paecilomyces japonica in the mulberry leaf extract. J. Life Sci. 17: 816-821. 2007 https://doi.org/10.5352/JLS.2007.17.6.816
  5. Choi, SY, Kang, NJ and Kim, HC. Inhibitory effects of root extracts on melanin biosynthesis in Rodgersia podophylla Gray. Kor. J. Medicinal Crop Sci. 14:27-30. 2006
  6. Han, DS, Jung, SJ, Kim, SH and Ahn, BH. Effect of tyrosinase inhibitors on the melanogenesis. Kor. J. Food Sci. Technol. 28: 1089-1094. 1996
  7. Chen, JS, Wei, C and Marshall, MR. Inhibition mechanism of kojic acid on polyphenol oxidase. J. Agric. Food Chem. 39:1897-1901. 1991 https://doi.org/10.1021/jf00011a001
  8. Lee, JS, Kim, JA, Cho, SH, Son, AR, Jang, TS, So, MS, Chung, SR and Lee, SH. Tyrosinase inhibitors isolated from the roots of Glycyrrhiza glabra. L. Kor. J. Pharmacogn. 34:33-39. 2003
  9. Kwak, JH, Kim, YH, Chang, HR, Park, CW and Han, YH. Inhibitory effect of gardenia fruit extracts on tyrosinase activity and melanogenesis. Kor. J. Biotechnol. Bioeng. 19:437-440. 2004
  10. Kwak, JH, Seo, UK and Han, YH. Inhibitory effect of mugwort extracts on tyrosinase activity. Kor. J. Biotechnol. Bioeng. 16:220-223. 2001
  11. Sung, KC and Kim, KJ. Tyrosinase activated inhibition effect & analysis of pine-needles extract. J. Kor. Oil. Chem. Soc. 22:71-76. 2005
  12. Yang, MJ, Lim, JS, Ahn, HS, Kim, MA and Ahn, RM. Inhibitory effect of chestnut bark extracts on tyrosinase activity and melanin biosynthesis. Kor. J. Env. Health Soc. 25:37-43. 1999
  13. Bang, BH, Rhee, MS, Kim, JO and Yi, DH. A study on the tyrosinase inhibitor produced with Actinomycetes F-97. J. Life Sci. 17:798-804. 2007 https://doi.org/10.5352/JLS.2007.17.6.798
  14. Suzuki, K. Search and discovery of soil microorganism which produce new bioactive substances: Selective isolation of microorganism and their fermentation products. J. Actinomycetol. 7:107-109. 1993 https://doi.org/10.3209/saj.7_107
  15. Kwon, OS, Park, DJ, Lee, CY and Kim, CJ. Distribution pattern of soil Actinomycetes at Cheju. Kor. J. Appl. Microbiol. Biotechnol. 4:399-403. 1996
  16. Shear, MC. Actinomycetes; pennanent preservation. In workshop on preservation of microorganism by freezing and freezedrying. Society for Industrial Microbiology, Arlington, Va. 1967
  17. Lee, CH, Onmg, MC, Lee, HJ, Lee, KH and Kho, YH. MR304-1, A melanin synthesis inhibitor produced by Trichoderma harzianum. Kor. J. Appl. Microbiol. Biotechnol. 6:641-646. 1995
  18. Shirling, EB and Gottlieb, D. Methods for characterization of Streptomyces species. Int. J. System. Bacteriol. 16:313-340. 1996 https://doi.org/10.1099/00207713-16-3-313
  19. Ballows, AH. Truper, G, Dworkin, M, Hander, W and Schlerifer, KS. The procaryotes. Vol. 1, 2nd ed., pp.67-80. SpringerVerlag. 1992
  20. Willims, S, Sharp, TME and Holt, JG. Bergey's manual of systematic bacteriolgy. vol. 4, pp.101-130. Williams and Wilkins, 1989
  21. Gerhardt, P, Murray, RGE, Wood, WA and Krrieg, NR. Methods for general and molecular bacteriology, pp.123-150. American Society for Microbiology, 1994