• 한국생물정보학회:학술대회논문집
• /
• 한국생물정보시스템생물학회 2002년도 제2차워크샵
• /
• pp.143-167
• /
• 2002

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제21권1호
• /
• pp.94-103
• /
• 2016

Landfarming that supplies aerobic biodegradation condition to indigenous microbes in soils is a biological remediation technology. In this research, volatilization and biodegradation rate by indigenous microbes in the soil contaminated with total petroleum hydrocarbons (TPH) were measured. Soils were contaminated with diesel artificially and divided into two parts. One was sterilized by autoclave to remove indigenous microorganism and the other was used as it was. Various moisture contents and number of tillings were applied to the soil to find out proper condition to minimize volatilization and enhance bioremediation. Volatilization of TPH was inhibited and biodegradation was enhanced by increase on moisture content. Tilling was usually used to supply air for microbes, but tillings did not affect the growth of microbes in our study. Enough moisture content and proper aeration are important to control volatilization in landfarming. Also, TPH degradation was a function of the microbe counts (x₁), numbers of tilling (x₂), and moisture content (x₃) from the application of the response surface methodology. Statistical results showed the order of significance of the independent variables to be microbe counts > numbers of tilling > moisture content.

• Journal of Microbiology and Biotechnology
• /
• 제14권1호
• /
• pp.212-215
• /
• 2004

In order to develop a biocontrol agent that can effectively control Fusarium wilt on Cymbidium genus, the effectiveness of antagonistic microbes against the cause pathogen was screened. The selected microbe showed a broad spectrum of antifungal activity, and the culture broth of this microbe had better preventive effect on Fusarium wilt than the commercial chemical agent in the pot assay. This isolated strain, GBA-12, was identified as Streptomyces kasugaensis, and the antifungal substance was purified from a broth culture of GBA-12. This purified substance was identified as a polyene macrolide (YS-822A) that was newly discovered from Streptomyces kasugaensis, and it exhibited antifungal activity against several phytopathogenic fungi.

• 한국미생물·생명공학회지
• /
• 제28권5호
• /
• pp.243-250
• /
• 2000

The study was performed to investigated the excellent microbial anticaries substance which is more effective than the chlorohexidine in the dental caries treatment. For the screening of alkaliphilic microorganism, more than 1200 bacterial strains were isolated from sea soil sample. A typ-ical strain which produced the most excellent antimicrobial substance was selected. The strain was identified novel alkalophilic Bacillus sp. through the results of morphological, biochemical and chemotaxonomical characteristics and 16S rDNA sequencing and designated as Bacillus alkalophilshaggy JY-827.

• 한국잠사곤충학회지
• /
• 제7권
• /
• pp.53-63
• /
• 1967

본 실험은 한국산 잠견에 기생하는 세균을 분리하여 생리학적 형태학적 및 배양적인 특성을 파악하여 그 미생물을 동정하고 저장중인 잠견에 미생물 침식을 방지하는 과학적인 근거를 모색했다. 그 실험결과는 다음과 같다. 1. 잠견으로부터 분리된 12 strain의 Bacteria의 형태학적인 실험결과 colony의 형태는 table II와 같다. 종합적인 형태적 특성은 table III과 같으며 gram stain과 spore stain은 Fig, I, II와 같다. 2. 분리된 12 strain의 배양적인 특성은 table IV, V, Ⅵ와 같다. 3. 분리된 12 strain의 생리적인 특성은 table Ⅶ와 같다. 4. 이상, 형태학적 배양적 생리적인 특성에 의거하여 분리균주의 유연관계를 추정하면 다음과 같다. (l) No 1 No 8; Bacillus subtilis variation (2) No 2 ; Bacillus stearothermophilus (3) No 3 ; Bacillus circulans (4) No 5 No 6; Bacillus thuringiensis (5) No 7 No 11; Bacillus brevis (6) No 12 No l0; Bacillus cereus variation

• Journal of Microbiology and Biotechnology
• /
• 제30권2호
• /
• pp.248-258
• /
• 2020

The vaginal microbiota may be important for pregnancy prognosis because vaginal dysbiosis during pregnancy appears to be related to preterm birth (PTB) or pregnancy loss. Previous reports have indicated that a Lactobacillus-poor microbial flora in the vagina and intrauterine infection by diverse anaerobes ascending from the vagina are associated with undesirable delivery outcomes. However, no research has involved the use of pyrosequencing analysis to examine vaginal microbiota profiles or their potential associations with high-risk pregnancy in Korean women. Vaginal swabs were collected from 500 Korean women for the identification of community state types (CSTs). Of these, 137 samples were further analyzed using a Roche/454 GS Junior pyrosequencer. Three distinct CSTs were identified based on the dominant vaginal microbes: CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), and CST IV (with diverse species of anaerobes). Twelve of the 67 pregnant women had undesirable pregnancy outcomes (four miscarriages and eight PTBs). The dominant microbe in the vaginal microbiota of women who gave birth at full-term was L. crispatus. In contrast, L. iners was the dominant vaginal microbe in women who miscarried. Most (n = 6/8) vaginal microbiota profiles of women who experienced PTB could be classified as CST IV, with diverse bacteria, including anaerobic vaginal species. The present study provides valuable information regarding the characteristics of the vaginal microbiota of Korean women related to high-risk pregnancy. Investigation of the vaginal microbiotic structure in pregnant Korean women is necessary to enable better prediction of adverse pregnancy outcomes.

• 한국컴퓨터정보학회논문지
• /
• 제10권1호
• /
• pp.113-124
• /
• 2005

세균검사실의 검사업무는 균종 및 검사종류가 다양하고 최소시간에 검사결과를 도출해야 하므로 업무내용이 복잡하다. 이러한 문제를 해결하기 위하여 에이전트를 이용하여 세균검사에 스케줄링 시스템을 구현하였고, 스케줄링을 관리하는 워크플로우 관리 시스템을 도입하였다. 스케줄링 체크를 위한 쿼리들은 조회가 가능하도록 궈리 시스템을 구현하였다. 그리고 세균검사의 중간 리포트나 결과리포트를 자동으로 보고할 수 있게 하였다. 구현된 스케줄링 시스템은 자원의 낭비나 부족을 파악하고 효율적인 자원할당 및 관리를 할 수 있었다. 궈리 시스템은 스케줄링 체크와 세균검사의 진척상황을 짧은 응답시간에 조회할 수 있었구 리포트의 자동보고 및 근거리나 원거리에서의 조회가 가능하고, 확인과정으로 인한 검사업무의 지연이나 방해요소를 줄일 수 있었다. 또한 검사기기의 오류나 배지, 시약의 부족 등 예측치 못한 예외사항에 대해 검사의뢰자가 확인할 수 있기 때문에 예외사항으로 발생할 수 있는 인간관계의 갈등 요소를 해결할 수 있었고, 작업진행의 효율화를 기대할 수 있었다.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1683-1684
• /
• 2008

For direct detection of microbes in air, samples have to be collected but environmental particles such as dust are also trapped in such samples. Therefore the isolation of target bacteria from non-biological materials of similar size is of great importance in the identification of such organisms. Dielectrophoresis is an emerging technique that can rapidly separate cells in microfluidics. In this paper we proposed a new method for the separation of airborne microbes using condensation and dielectrophoresis. This system could be used as a continuous flow through separation system for various particles and utilized as a pretreatment technique for microbe detection.

• Journal of Dairy Science and Biotechnology
• /
• 제39권3호
• /
• pp.113-120
• /
• 2021

Although the number of incidences of illness caused by ingestion of the bacterial pathogen Enterobacter sakazakii (Cronobacter spp.) has dramatically declined, there remains a need for a robust isolation method to recover this microbe from powdered infant formula (PIF). The current method described in the FDA's Bacteriological Analytical Manual requires multiple steps, and 3-4+ days for complete analysis of PIF isolated E. sakazakii (Cronobacter spp.). We describe a bacteriological method including a one-step enrichment followed by plating on chromogenic agar for presumptive identification of E. sakazakii (Cronobacter spp.). Suspected colonies are confirmed by either biochemical analyses, or a Real-Time PCR-based assay. Using this method, E. sakazakii (Cronobacter spp.) in PIF can be isolated and identified within one day (24 hours).

• 한국미생물·생명공학회지
• /
• 제45권2호
• /
• pp.124-132
• /
• 2017

오늘날 해양생물로부터 얻어진 미생물유래의 이차대사물질은 구조적, 생물학적으로 새로운 화합물의 주요한 자원이다. 그 중에서 해면동물과 미생물 관계는 생리활성 물질을 탐색하는데 가장 흥미있는 자원 중 하나로서 주목받아 왔다. 본 연구에서는 서해안 조간대에서 채집된 주황해변해면(Hymeniacidon sinapium)으로부터 분리된 세균 균주(Microbulbifer sp., 127CP-12)를 검토하였다. 배양된 세균은 자주색 색소를 생산하였으며, 색소생산의 최적 배양조건을 조사하였다. 최대 색소생산을 위한 미생물 배양조건은 $25^{\circ}C$, pH 6.0, 3% NaCl임을 알 수 있었다. 추출용매는 에탄올과 메탄올에 비해 아세톤이 더 적절한 것으로 나타났다. 추출된 색소의 주요성분은 HLPC, NMR, MS, 그리고 UV 스펙트럼의 구조 분석을 통해 유용한 생리활성물질인 비올라세인으로 밝혀졌다. 본 연구는 해양미생물이 관여한 대사물질로부터 생리활성물질을 조사하는 연구기법을 서술함과 동시에 오늘날 변화하는 해양환경에서 해면동물과 미생물 관계의 생태학적 의의를 제시하고 있다.