• The Korean Journal of Malacology
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• v.30 no.2
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• pp.117-126
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• 2014

To assess the effect of environmental factors on the sustainability of cultured production manila clam (Ruditapes philippinarum), we investigated the habitat characteristics of tidal flat (Hwangdo and Jeongsanpo in Taean). We measured the physiochemical parameters (temperature, salanity, pH, dissolved oxygen and nutrients) and the characteristics (mean size, chemical oxygen demand, ignition loss, C/N ratio and C/S ratio) of surface sediments. The C/N ratio of Hwangdo and Jeongsanpo were 9.0, 5.3 and the C/S ratio was 0.162, 0.159. The concentration of chlorophyll a at Hwangdo was higher than that of Jeongsanpo and species of micro algae were 102 and 100. The enrichment factor (Ef) and index of accumulation rate (Igeo) of the metals showed that those research areas can be classified as heavily polluted, heavily to moderately polluted, or more or less unpolluted, respectively. So two survey area of cultured clams in the tidal flat have been effected by the various environmental conditions, there are needed an improvement methods and continuous research for increasing the production of manila clam (Ruditapes philippinarum).

• Microbiology and Biotechnology Letters
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• v.43 no.3
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• pp.275-279
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• 2015

Micro-algae are unicellular photosynthetic organisms and produce pigments such as chlorophyll and carotenoid. Chlorella contains a lot of protein and functional components like lipids, chlorophyll and carotenoids. In this study we induced mutants of Chlorella vulgaris (C. vulgaris) through ultraviolet radiation (UV-B) and selected two mutants by pigment (chlorophyll and carotenoids) content. We named the mutants ‘UBM1-2’, ‘UBM2-57’ and they were cultivated for 21-days. Cell growth, dry cell weight, protein content, lipid and pigments content were measured. The results indicated that the mutants displayed slower cell growth, lower dry cell weight and protein content than the wild type. However, for UBM1-2 the lipid content was 21% higher than the wild type. In addition, the mutants’ chlorophyll content was 37% and 89% higher than the wild type and the carotenoids content was 27% and 70% higher than the wild type, respectively.

• Journal of the Korean GEO-environmental Society
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• v.15 no.8
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• pp.5-11
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• 2014

In this study, chemical analysis and ecotoxicity tests of leachate from disposal site for foot-and-mouth disease carcasses (FMD leachate) were conducted to collect fundamental data that will be used to develop environmental risk assessment tools for FMD leachate. For chemical analysis, concentration of $Cl^-$, $NH{_4}{^+}-N$, Korea standard method indicators for detection of leachate released from animal carcasses burial site into groundwater and NRN (Ninhydrin-Reactive Nitrogens), a newly suggested screening test indicator to detect groundwater contamination by FMD leachate, were assessed. For ecotoxicity tests, luminescent bacteria (V. fischeri), micro-algae (P. subcapitata) and water flea (D. magna) were selected as test species. Correlation analysis between the concentration of $Cl^-$, $NH{_4}{^+}-N$, NRN and the toxicity to V. fischeri was performed to identify the better indicators to monitor FMD leachate contamination. From regression analysis, the concentration of the indicators in FMD leachate contaminated sample that induced halfmaximal toxic effect to V. fischeri was evaluated. Results obtained from this study can be applied to assess the risk by FMD leachate and to establish the guideline to manage risk in relation to FMD leachate.

• Journal of the Korea Organic Resources Recycling Association
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• v.7 no.2
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• pp.57-64
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• 1999

The purpose of this study is to establish effective conditions for controlling $CH_4$, $N_2O$ emission from organic Waste / wastewater treatment processes. Continuous and batch experiments were conducted to treat the micro algae from polluted and eutrophicated lakes through the thermophilic oxic process. The microalgae used were mainly Microcystis sp.(collected from eutrophic lake) and Chlorella sp. (cultured in laboratory) Wasted cooking oil was added by aid-heating source. Physico-chemical components of sludges and microalgae were analyzed. In batch experiments, air supply was changed from 50ml/min to 150ml/min. The temperature. water content and drained water were affected by the air flow rate at initial stage. However, there was almost no influence of air flow rate on them in middle and last stages. At air flow rate of 100ml/min, the degradation rate of organic material was higher than that at other air flow rates. $CO_2$ concentration in exhaust was proportional to the strength of aeration, especially at initial stage when degradation was active. $CH_4$ with low concentration was detected only at starting stage when air diffusion was not enough. $N_2O$ production was not affected by variation of air supply. In continuous experiments no matter what the dewatering methods (with PAC and without PAC) and media (wood chip and reed chip) were changed, $N_2O$ was almost not affected by variation of injected air. Result showed that the reed chips using for lake purification could be used as media for thermophilic oxic process in lake and marshes area. $CO_2$ concentration was not so much affected by the change of dewatering methods and media types. $CH_4$ was not detected in the experimental period. So it can be shown that the thermophilic oxic process had been well operated in wide handling conditions regardless of media and dewatering methods.

• Membrane Journal
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• v.24 no.1
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• pp.31-38
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• 2014

Rainwater harvesting is a process by which rainwater collected from rooftop or other catchment is purified so that the water can be directly or indirectly used by human beings for beneficial uses. As rainwater is increasingly considered for high quality purposes, membranes have gained an important place in rainwater treatment. It has advantages such as the production of high quality water, small footprint, and affordable energy consumption. Nevertheless, membrane fouling is regarded as a serious problem similar to the cases of water treatment and wastewater reclamation. In this study, we applied microfiltration (MF) membranes for rainwater treatment. In addition, a low pressure ultraviolet (UV) process was also use as a pretreatment to control notonly. To quantify the effect of UV on organic matters, both total organic carbon (TOC) and UV absorbance (UVA) were measured. Moreover, the effect of UV pretreatment on membrane fouling was investigated. Experimental results indicated that the pretreatment of membranes using LPUV was effective to control fouling of MF membranes only when the rainwater was contaminated by algae. This was attributed the reduction and modification of organics after UV treatments. It is likely that the UV/MF process is a promising option for water treatment in decentralized water treatment such as micro water grid systems.

• Food Science and Preservation
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• v.24 no.5
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• pp.673-679
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• 2017

In this study, the antimicrobial, antioxidant activities and ${\alpha}$-glucosidase inhibitory activities of Amphidinium carterae ethanol extract (AE) was evaluated for using as a functional food ingredient. Chlorella ethanol extract (CE) was used to the comparison as a control. Anticancer activities of the AE and CE were analyzed by HepG2 and HT-29 human cancer cell. The AE showed antimicrobial activities for all tested bacterial strains. Whereas, CE showed antimicrobial activities for several tested bacterial strains only. The CE showed higher total phenolics contents, DPPH and ABTS radical-scavenging activities (47.36 mg/g, 22.42% and 28.58%, respectively) than those of AE (8.88 mg/g, 20.16% and 17.69%, respectively). AE showed anti-diabetic effect on ${\alpha}$-glucosidase inhibitory activity with dose-dependantly manner. The cell viability of AE ($125{\mu}g/mL$) on HepG2 and HT-29 human cancer cells were 38.12% and 11.27%, respectively. It was demonstrated that ethanol was efficient solvent for extracting functional components from A. carterae. These results indicated that AE can be described as a good candidate for using as a functional food ingredient.

• Korean Journal of Ecology and Environment
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• v.57 no.2
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• pp.61-74
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• 2024

Daemadeung, located in the estuary of the Nakdong River, is formed by sand dunes and possesses well-developed intertidal flats. This study aimed to investigate the habitat of benthic microalgae, photosynthetic pigments, and photosynthetic efficiency in the intertidal flats of Daemadeung from January to December 2011. The inorganic nitrogen content in the sediment pore water was primarily composed of ammonium, while nitrate + nitrite was dominant in the upper layer water. The concentration of chlorophyll a and fucoxanthin in the sediment surface was significantly higher than the mean of all the sediment layer. The average Fv/Fm of benthic microalgae during the entire survey period was 0.52±0.03, with the highest value (0.61±0.08) observed in February. The rETR_max showed a seasonal trend, being high from spring to early autumn (April to October) and low from winter to early spring (January to March, November, December), with the highest value (153.05±2.30 µmol electrons m^-2 s^-1) in July and the lowest (38.49±5.17 µmol electrons m^-2 s^-1) in January. The average Fv/Fm of diurnal microalgae was 0.48±0.03, with the highest value (0.61±0.08) observed at noon. The rETR_max showed a highest peak at noon (54.24±11.35 µmol electrons m^-2 s^-1) and reached its lowest point at 16:00 (26.17±4.75 µmol electrons m^-2 s^-1). These findings suggest that the productivity of benthic microalgae varies significantly depending on the survey time and sediment depth. Therefore, to quantify the productivity of benthic microalgae using Diving-PAM, surveys should be conducted based on tidal conditions, and simultaneous pigment analysis of sediment layers should also be performed.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.4
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• pp.287-301
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• 2014

In order to understand environment condition and phytoplankton community before and after coastal upwelling, the influences of upwelling events on phytoplankton community were studied at 18 stations located the Southern part of East Sea, Korea from May to August 2013. The surface water masses showed low temperature and high salinity due to upwelling events at coastal stations (A1, B1, C1). Correlation between temperature and nutrients (DIP, r=-0.218, p<0.01; DIN, r=-0.306, p<0.01; silicate, r=-0.274, p<0.01) was significantly negative. This result could be explained that nutrients were supplied to surface water by the upwelling of bottom water. Phytoplankton communities were composed of 186 species. Phytoplankton abundance were relatively high in May (C1, $726{\times}10^3cells\;L^{-1}$) and July (A1, $539{\times}10^3cells\;L^{-1}$). Total chlorophyll a and micro-size fraction ($&gt;20{\mu}m$) increased at coastal stations in July and August, while phytoplankton abundance and total chl. a was much low in June. Dominant species in June was Pseudo-nitzschia spp. of which the cell size was $309{\mu}m^3$. Cell size of Pseudo-nitzschia spp. was smaller than dominant species in other period. Therefore, the increase in total chloro-phyll a and the size of phytoplankton was resulted in the sufficient supply of nutrients. In contrast, these tendencies were not observed at outside stations. These results suggested that coastal upwelling was an important influencing factor to determine the species composition and standing stock of phytoplankton community in the coastal waters of the Southern part of East Sea, Korea.

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.231-240
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• 2016

Techniques were developed for holding and conditioning of Pacific oysters, Crassostrea gigas, in a closed recirculating system. Experimental adults were used 500 oysters ( x two system, total 1,000 oysters) which were collected in $20^{th}$ March 2016 from long-line aquaculture farm at the south coast of Korea. During conditioning periods concentrated live microalgae as Isochrysis sp. $15{\times}10^7cells/mL$, Tetraselmis sp. $2{\times}10^7cells/mL$ and Pheaodactylum sp. $18{\times}10^7cells/mL$ were added 5 L every day, respectively which micro algae were functioned as diets and biological filter. Over all experimental periods total water exchange rate was 21.3% (daily 0.5%). Over 42 days conditioning, female and male oysters were maturated 90.9% and 94.4%, respectively. Survival rate was 98.7%. Mean shell hight (8.3 mm), total wet weight (19.2 g), meat wet weight (5.0 g) and shell wet weight (13.6 g) were significantly increased (P < 0.05). Water quality parameters including the water temperature ($22.1{\pm}0.4^{\circ}C$), salinity ($24.9{\pm}04$), dissolved oxygen (5.1-7.9 mg/L) and pH ($7.93{\pm}0.15$) were kept stable. Concentration of dissolved inorganic nutrient as ammonia (1.96-0.35 mg/L), nitrite (0.03-0.16 mg/L), nitrate (1.34-0.47 mg/L), DIP (0.42-0.03 mg/L) and silicate (3.83-0.00 mg/L) were significantly decreased throughout experiment except nitrite which was increased (P < 0.05), but nitrogenous components stayed below toxic levels (ammonia 0.0-5.5 mg/L, nitrite 0.0-460.0 mg/L) which indicated that closed recirculation system with microalgae based bio-filter could supply sufficiently environment condition to holding and conditioning of oyster.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.21 no.1
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• pp.24-35
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• 2016

To assess the relationship between environmental factors and seasonal phytoplankton community structure, we investigated abiotic and biotic factors in Ulsan Bay, Korea. We divided the bay into two areas based on geographical characteristics and compared the difference in each factor between inner and outer bay with t-test statistics. As a result, temperature in the outer bay was higher than that of the inner bay during winter (t = -5.833, p < 0.01) and autumn (p > 0.05). However, opposite trend was observed during spring (t = 4.247, p < 0.01) and summer (t = 2.876, p < 0.05). Salinity was significantly lower in the inner bay than in the outer bay in winter, spring, and summer (p < 0.01). However, the salinity was not significantly different between the inner and the outer bay in the autumn (p > 0.05). In particular, high nutrient concentration was observed in most stations during winter season due to vertical well mixing. The nutrient concentration was significantly higher in surface layers of inner bay after rainfall, particularly in the summer. The relative contribution (approximately 70%) of < $20{\mu}m$ (nano and pico) size phytoplankton was increased in all seasons with continuously low nutrients from the offshore water due to their adaption to low nutrient without other large competitors. Interestingly, high population of Eutreptiella gymnastica was kept in the inner bay during the spring and summer associated with high DIN (nitrate+nitrite, ammonium) after river discharge following rainfall, suggesting that DIN supply might have triggered the increase of Eutreptiella gymnastica population. In addition, high density of freshwater species Oscillatoria sp. and Microcystis sp. were found in several stations of the inner bay that were provided with large amounts of freshwater from the Tae-wha River. Diatom and cryptophyta species were found to be dominant species in the autumn and winter. Of these, centric diatom Chaetoceros genus was occupied in the outer bay in the autumn. Cryptophyta species known as opportunistic micro-algae were found to have high biomass without competitors in the inner bay. Our results demonstrated that Ulsan Bay was strongly affected by freshwater from Tae-wha River during the rainy season and by the surface warm water current from the offshore of the bay during dry season. These two external factors might play important roles in regulating the seasonal phytoplankton community structures.