• Molecules and Cells
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• v.28 no.6
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• pp.521-527
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• 2009

Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.

• BMB Reports
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• v.48 no.7
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• pp.371-372
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• 2015

MicroRNAs (miRNAs) can regulate the expression of genes that are involved in multiple cellular pathways. However, their targets and mechanism of action associated with the autophagy pathway are not fully investigated yet. EWSR1 (EWS RNA-Binding Protein 1/Ewing Sarcoma Break Point Region 1) gene encodes a RNA/DNA binding protein that is ubiquitously expressed and plays roles in numerous cellular processes. Recently, our group has shown that EWSR1 deficiency leads to developmental failure and accelerated senescence via processing of miRNAs, but its role in the regulation of autophagy remains elusive. In this context, we further investigated and found that EWSR1 deficiency triggers the activation of the DROSHA-mediated microprocessor complex and increases the levels of miR125a and miR351, which directly target Uvrag. Interestingly, the miR125a- and miR351-targeted reduction of Uvrag led to the inhibition of autophagy in both ewsr1 knockout (KO) MEFs and ewsr1 KO mice. In summary, our study demonstrates that EWSR1 is associated with the posttranscriptional regulation of Uvrag via miRNA processing. The regulation of autophagy pathway in miRNAs-Uvrag-dependent manner provides a novel mechanism of EWSR1 deficiency-related cellular dysfunction. [BMB Reports 2015; 48(7): 371-372]

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.372-373
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• 2012

• Journal of Digital Convergence
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• v.16 no.9
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• pp.347-357
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• 2018

The purpose of this research is to investigate the relationship between global citizenship, self leadership and global health competencies in nursing students and utilize the basic data for development of educational program to increase of global health competence. A convenience sample of 207 subjects were invited from three university. Data were analyzed using t-test, ANOVA and Pearson's Correlation with SPSS PC 18.0 program. Global health competencies showed that there were differences according to religion, taking education related to global health competency, exposure to mass media about global health. There were a significant positive correlation of global citizenship(r=.364, p<.01), self leadership(r=.372, p<.01) and the global citizenship of nursing students showed a significantly positive correlation of self leadership(r=.463, p<.01). Therefore, on the result of this study, when we develop the educational programs to increase the global health competencies for nursing students, we have to include global citizenship and self leadership.

• Journal of Industrial Convergence
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• v.20 no.1
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• pp.77-86
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• 2022

This study is a descriptive research study to understand the level of communication awareness between doctors and nurses, who are professional medical professionals, and the detailed areas and satisfaction of communication. Data were collected from 372 doctors and nurses at general hospitals located in C city from March to May 2021. The collected data were analyzed with descriptive statistics, mean and standard deviation, t-test, ANOVA, Scheffe test, and correlation analysis, using the SPSS/WIN 20.0 program. As a result, there was a difference in the awareness level of communication between doctor and nurse groups. Specifically, out of the detailed areas of communication recognized by doctors and nurses, there were statistically significant differences in openness(t=9.91), mutual understanding between occupations(t=5.25), and satisfaction(t=8.13) between the two groups. In addition, a positive correlation was found between the detailed areas and the communication satisfaction in both groups, showing that nurses have higher communication satisfaction with the higher openness(r=.72, p<.001), mutual understanding between occupations(r=.71, p<.001) and similarly, doctors also have higher communication satisfaction with the higher mutual understanding between occupations(r=.79, p<.001), timeliness(r=.73, p<.001). Therefore, these result suggest that it is necessary to develop a communication program that can effectively improve the weak areas such as mutual understanding between occupations and openness in nurses and doctors in order to ensure patient safety and provide quality medical care.

• Journal of Convergence for Information Technology
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• v.8 no.3
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• pp.127-136
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• 2018

This study was to identify factors influencing satisfaction on clinical practice of dental hygienist students located in G, J region. The data was analyzed by t-test, Anova, Pearson correlation coefficient, and multiple regression using SPSS 21.0 program. There were significant in satisfaction of score(F=2.925, p<.05), perceive heath status(F=8.108, p<.001), satisfaction on department(F=8.198, p<.001). The relationship on peers with score(r=.277, p=.01) and perceive health status correlations with relationships on peers(r=.327, p=.01), The satisfaction on clinical practice had positive correlations with score(r=.127, p=.05), perceive health status(r=.226, p=.01), relationship on peers(r=.240, p=.01). The factors influencing satisfaction on clinical practice were satisfaction of relationship on peers, satisfaction of department, perceive health status. The results suggest that development of program to improve satisfaction on clinical practice of dental hygiene students.

• Journal of Korean Academy of Nursing Administration
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• v.12 no.3
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• pp.365-372
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• 2006

Purpose: OSCE, Objective Structured Clinical Examination, is a good way to evaluate clinical skills of nursing students. To do this, we need modules, evaluators, persons to run the examination, as well as models and standardized patients if necessary. Author coached nursing students to develop modules and ran the examination by themselves. Method: 24 third-year and 4 first-year students volunteered; third-year students developed 5 modules, and organized and ran the examination. First-year students played patient role. 60 2nd-year students participated as examinees. Modules were duplicated to finish examination in a given time. The relationship between OSCE score, conventional clinical evaluation score and credit of students was compared. Effect of module duplication on score was tested. And responses of examinees were collected. Results: There was no correlation between OSCE and conventional clinical evaluation score (r=0.07), and credit (r=0.27), And there was no difference of OSCE score between duplicated modules $(53.77{\pm}7.61$ vs $55.33\pm7.74).$ Response of examinees to OSCE was favorable. Examinee did not expressed resistance for the evaluation by OSCE developed and ran by students. Conclusion: Nursing students successfully developed and ran OSCE, which was accepted favorably by examinees. Student-developed OSCE may play a role in evaluation of clinical performance.

• Archives of Plastic Surgery
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• v.36 no.4
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• pp.372-379
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• 2009

Purpose: The purpose of this study was to compare the effect on antibacterial activities and wound healing effect of silver containing dressings with which of Betadine against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: One full thickness skin defects in rats(n=72) were developed on the back and were given rise to infection with S. aureus(n=36) and P. aeruginosa(n=36). The 72 mice were divided into 6 groups : Acticoat$^{(R)}$, Aquacel$^{(R)}$-Ag, Medifoam silver$^{(R)}$, Polymen silver$^{(R)}$, Ilvadon$^{(R)}$ and Betadine(control group) dressing groups. Five silver containing dressings and Betadine were assesed on infected wound. Measurement of wound size change, bacterial colonies count and histologic findings was applied. Antibacterial activity was analyzed with bacterial restricted zone in Mueller Hinton agar. Results: On S. aureus, wound size was more decreased in all treated groups as compared with betadine, however Ilvadon$^{(R)}$ was less decreased on P. aeruginosa. In histologic findings, experimental group showed more effective findings than others on S. aureus, however on P. aeruginosa, which was showed similar. Acticoat$^{(R)}$ was best effective in wound healing against S. aureus and P. aeruginosa. The bacterial colonies count was increased in all treated groups except Acticoat$^{(R)}$ as compared with the control group on S. aureus, which was decreased in Acticoat$^{(R)}$ and Ilvadon$^{(R)}$ group on P. aeruginosa. There were not statistical differences. The restricted zone was shown in Mueller - Hinton agar of all groups except Medifoam silver$^{(R)}$ group on S. aureus, which was shown of all groups on P. aeruginosa. There were statistical differences. Conclusion: This study suggests that silver containing dressings may have not better potential than Betadine in assisting management of wounds at risk of infection on S. aureus and P. aeruginosa. However, which have better antibacterial activity on S. aureus and P. aeruginosa.

• Journal of the Korean Applied Science and Technology
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• v.38 no.6
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• pp.1393-1404
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• 2021

The current study was conducted to understand the status of infection management through a survey on the status of awareness and performance of infection management and to improve efficient infection management and education programs of workers in long-term care institutions. The subjects of the study were 134 workers at 35 long-term care institutions in the C region, and data were collected from July 12 to 30, 2021. They agree to participate in research self reported structured questionnaire by SPSS program ver. 22.0. As a result of the study, the subject's awareness of infection management appeared to be a difference in accordance with occupation (F=3.181, p=.032) and education experience (F=6.372, p=.013). Also, factors influencing the subject's performance of infection management were occupation (F=3.972, p=.010) and education experience (F=4.403, p=.038). The relationship between awareness of infection management and performance resilience fined out a significantly positive correlation (r=.919, p<.001). As a result, for patient safety in long-term care institutions in the COVID-19 pandemic situation, it is very important for workers not only to recognize the importance of infection management but also to directly perform infection management activities. To this end, regardless of the size of the institution, it is necessary to prepare a systematic and continuous curriculum by occupation and educational topic, and to legally strengthen the evaluation system to improve the quality of the infection management system.

• Journal of Digital Convergence
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• v.16 no.5
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• pp.361-372
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• 2018

The purpose of this study was to develop an assessment scale for healthy aging of middle aged women. The data were collected from 385 middle aged women in two large cities in Korea. The development processes of the scale were followed: Development of preliminary items; Verification of content validity and reliability; Factor analysis and criterion validity of final items. Results: Factor analysis resulted in the creation of the final scale, which consisted of 38 items that were grouped into 9 factors: self achievement, physical change adaptation, psychological change adaptation, interpersonal relationships, menopausal symptom adaptation, economic stability, physical health, social support, social change adaptation. The explanatory variance was 64.03%. The reliability of the scale was Cronbach's ${\alpha}=.90$. Correlation of the scale with healthy aging and depression, established its construct and concurrent validity was r=-.69, p<.001. The scale was confirmed to have a relatively high validity and reliability. The findings of the study can be useful as a measurement to evaluate healthy aging of middle aged women. Future researches with the scale will be helpful for basic data for the program development to support healthy aging of middle aged women.