Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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miR-372 Regulates Cell Cycle and Apoptosis of AGS Human Gastric Cancer Cell Line through Direct Regulation of LATS2

  • Cho, Wha Ja (Biomedical Research Center, Ulsan University Hospital, University of Ulsan College of Medicine) ;
  • Shin, Jeong Min (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Kim, Jong Soo (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Lee, Man Ryul (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Hong, Ki Sung (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Lee, Jun-Ho (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Koo, Kyoung Hwa (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine) ;
  • Park, Jeong Woo (Department of Biological Sciences, University of Ulsan) ;
  • Kim, Kye-Seong (Department of Anatomy and Cell Biology, Hanyang University, College of Medicine)
  • Received : 2009.05.21
  • Accepted : 2009.09.22
  • Published : 2009.12.31
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Abstract

Previously, we have reported tissue- and stage-specific expression of miR-372 in human embryonic stem cells and so far, not many reports speculate the function of this microRNA (miRNA). In this study, we screened various human cancer cell lines including gastric cancer cell lines and found first time that miR-372 is expressed only in AGS human gastric adenocarcinoma cell line. Inhibition of miR-372 using antisense miR-372 oligonucleotide (AS-miR-372) suppressed proliferation, arrested the cell cycle at G2/M phase, and increased apoptosis of AGS cells. Furthermore, AS-miR-372 treatment increased expression of LATS2, while over-expression of miR-372 decreased luciferase reporter activity driven by the 3' untranslated region (3' UTR) of LATS2 mRNA. Over-expression of LATS2 induced changes in AGS cells similar to those in AGS cells treated with AS-miR-372. Taken together, these findings demonstrate an oncogenic role for miR-372 in controlling cell growth, cell cycle, and apoptosis through down-regulation of a tumor suppressor gene, LATS2.

Keywords

Acknowledgement

Supported by : Ministry of Health and Welfare, Korea Research Foundation, Stem Cell Research Center

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