• Title/Summary/Keyword: miR-23a

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Genetic and Phenotypic Diversity of (R/S)-Mecoprop [2-(2-Methyl-4- Chlorophenoxy)Propionic Acid]-Degrading Bacteria Isolated from Soils

  • Lim, Jong-Sung;Jung, Mee-Kum;Kim, Mi-Soon;Ahn, Jae-Hyung;Ka, Jong-Ok
    • Journal of Microbiology
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    • v.42 no.2
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    • pp.87-93
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    • 2004
  • Twelve mecoprop-degrading bacteria were isolated from soil samples, and their genetic and phenotypic characteristics were investigated. Analysis of 16S rDNA sequences indicated that the isolates were related to members of the genus Sphingomonas. Ten different chromosomal DNA patterns were obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences from the 12 isolates. The isolates were found to be able to utilize the chiral herbicide meco-prop as a sole source of carbon and energy. While seven of the isolates were able to degrade both (R)-and (S)-mecoprop, four isolates exhibited enantioselective degradation of the (S)-type and one isolate could degrade only the (R)-enantiomer. All of the isolates were observed to possess plasmid DNAs. When certain plasmids were removed from isolates MPll, MP15, and MP23, those strains could no longer degrade mecoprop. This compelling result suggests that plasmid DNAs, in this case, conferred the ability to degrade the herbicide. The isolates MP13, MP15, and MP24 were identified as the same strain; however, they exhibited different plasmid profiles. This indicates that these isolates acquired dif-ferent mecoprop-degradative plasmids in different soils through natural gene transfer.

The study on the state of the elderly oral health and food habits (노인의 식습관과 구강건강상태에 관한 연구)

  • Gwon, Mi-Young;Won, Young-Soon;Kim, Yoon-Shin
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.2
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    • pp.13-24
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    • 2009
  • The purpose of this study was to examine the relationship between the dietary habits and oral health of elderly people in an attempt to pave the way for the development of oral health promotion programs geared toward improving the quality of life of the elderly. The subjects in this study were senior citizens who were selected by convenience sampling from Seoul. The findings of the study were as follows: 1. Regarding subjective oral health state, 54.5% of the elderly people, more than the half, considered their mouth to be in good health. The number of their mean remaining permanent teeth was 13.71. 47.5 percent of the senior citizens investigated had no shaking teeth. 2. Those who found themselves to be in good oral health had meals on a regular basis(p=0.022) and ate detergent food often. The gaps between them and the others was significant(p=0.005). In contrast, the elderly people who were in a bad oral health frequently ate cariogenic food(p=0.044). 3. The elderly people who had 21 teeth or more ate detergent food often(p=0.029), and those who owned no teeth had a sweet teeth(p=0.003), ate more cariogenic food(p=0.001) and had a snack frequently(p=0.026). 4. The subjective oral health status had a positive correlation to detergent food intake(r=0.23) and had a slightly negative correlation to preference for sweets(r=-0.14), cariogenic food intake(r=-0.14) and snack intake(r=-0.06). The number of tooth was positively correlated to detergent food intake(r=0.23) and negatively to preference for sweets(r=0.32), cariogenic food intake(r=-0.30) and snack intake(r=-0.21). The presence or absence of shaking teeth had a positive correlation to snack intake(r=0.14). The above-mentioned findings suggested that the dietary habits of the elderly people had a statistically significant relationship to subjective and objective oral health state, which indicated that there was a close relationship between oral health and dietary habits. Therefore how to improve dietary habits as well as oral health should be taken into account when oral health promotion programs are developed for the sake of the elderly. That would contribute to promoting the oral health of elderly people and eventually boosting their quality of life.

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Design of a Simple PCM Encoder Architecture Based on Programmable ROM (프로그래머블 ROM 기반의 심플 PCM 엔코더 설계)

  • Kim, Geon-Hee;Jin, Mi-Hyun;Kim, Bok-Ki
    • Journal of Advanced Navigation Technology
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    • v.23 no.2
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    • pp.186-193
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    • 2019
  • This paper presents and implements a simple programmable PCM encoder structure uisng the commutation method. In the telemetry system, information is required to assign each data to the channel in order to generate a frame format the data acpuired from the sensor. In this case, when the number of state information is large or the data type is various, there is a necessity to input a large amount of information to each channel. However, the more the number of channels and data, the more probability the error will occur. Therefore, in this paper, the channel information is created using the program. And PCM encoder was implemented to store channel information in ROM. The proposed PCM encoder architecture reduces the likelihood of errors. And it can improve the development speed. The validity of proposed structure is proved by simulation.

Knowledge, attitude, and preventive action on COVID-19 infectious disease of cosmetology major and arts and physical education college students (뷰티전공과 예체능전공 대학생의 COVID-19에 대한 지식, 태도, 예방실천행위)

  • Ahn, Mi Jeung;Park, Sun Ju
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.4
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    • pp.73-80
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    • 2021
  • This study is a descriptive cross-sectional study to confirm the knowledge, attitudes, and preventive practice behaviors of university students concerning COVID-19 and to identify the factors that influence preventive practice behaviors. The number of study subjects was calculated using G*power program 3.19. The data collected from the study were analyzed using SPSS/WIN version 25. The subject's characteristics, knowledge, attitude, and preventive practices against COVID-19 were analyzed with descriptive statistics, and the difference in knowledge, attitude, and prevention practices against COVID-19 according to the subject's characteristics was evaluated using an independent t-test, ANOVA, and post-hoc test was conducted using a Scheffé test. Correlations of knowledge, attitude, and prevention practices against COVID-19 were analyzed using Pearson Correlation coefficients, and factors affecting COVID-19 prevention practices were analyzed using stepwise multiple regression. The number of subjects analyzed in the study was 232. The general characteristics of the study subjects were 78 males (33.7 %) and 154 females (66.3 %). The subject's knowledge score about COVID-19 was 85.13±6.22 out of 100 points, and the attitude score toward COVID-19 was 12.47±1.31 out of 14 points. The prevention practice score for COVID-19 was 29.36±3.42 out of 32 points. As a result of the study, knowledge about COVID-19 was found to have a significant positive correlation with attitude (r=.34, p<.001); COVID-19 prevention practice behavior was found to have a significant positive correlation with knowledge (r=.54, p<.001), and attitude (r=.62, p<.001). The main factors influencing the COVID-19 prevention practice behavior were knowledge, attitude, and major (Cosmetology major).

A Study on Oriental Medicine Diagnostic Application through Analysis of Heart Rate Variability in Polycystic Ovary Syndrome Females (PCOS 여성의 HRV 특성 분석을 통한 한의학적 진단 활용성에 관한 연구)

  • Lee, Mi-Joo;Hwang, Deok-Sang;Lee, Jin-Moo;Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Khung-Sub
    • The Journal of Korean Obstetrics and Gynecology
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    • v.23 no.4
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    • pp.155-163
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    • 2010
  • Purpose: The aim of the study was to compare the characteristics of the autonomic innervation of the heart in polycystic ovary syndrome(PCOS) patients with regulary cycling controls. Methods: We studied 21 patients visiting $\bigcirc\bigcirc$hospital from 25th June 2009 to 25th June 2010. The subjects were categorized in two groups, 11 PCOS patients and 10 healthy regularly cycling controls. We studied the difference of Heart rate variability (HRV) between two groups by Independent samples T-test using SPSS for windows(version 17.0). Results: The Standard deviation of all normal R-R intervals (SDNN), The spuare root of the sum of the spuare of difference between adjacent normal R-R intervals (RMS-SD) of PCOS group was non-significantly lower than non-PCOS group. High frequency power (HF) and Normalized high frequency power (HF norm) of PCOS group was significantly higher than non-PCOS group. Normalized low frequency power (LF norm) of PCOS was signficantly lower than non-PCOS group. The results means increased sympathetic and decreased vagal modulation. Total power (TP), Very low frequency power (VLF) of PCOS group was non-significantly lower than non-PCOS group. Low frequency power (LF), LF/HF ratio of PCOS group was non-significantly higher than non-PCOS group. Conclusion: The results suggest that PCOS can be related to decreased activity of parasympathetic nervous system.

Cardamonin Suppresses TGF-β1-Induced Epithelial Mesenchymal Transition via Restoring Protein Phosphatase 2A Expression

  • Kim, Eun Ji;Kim, Hyun Ji;Park, Mi Kyung;Kang, Gyeung Jin;Byun, Hyun Jung;Lee, Ho;Lee, Chang Hoon
    • Biomolecules & Therapeutics
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    • v.23 no.2
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    • pp.141-148
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    • 2015
  • Epithelial mesenchymal transition (EMT) is the first step in metastasis and implicated in the phenotype of cancer stem cells. Therefore, understanding and controlling EMT, are essential to the prevention and cure of metastasis. In the present study, we examined, by Western blot, reverse transcription polymerase chain reaction (RT-PCR), and confocal microscopy, the effects of cardamonin (CDN) on transforming growth factor-${\beta}1$ (TGF-${\beta}1$)-induced EMT of A549 lung adenocarcinoma cell lines. TGF-${\beta}1$ induced expression of N-cadherin and decreased expression of E-cadherin. CDN suppressed N-cadherin expression and restored E-cadherin expression. Further, TGF-${\beta}1$ induced migration and invasion of A549 cancer cells, which was suppressed by CDN. TGF-${\beta}1$ induced c-Jun N-terminal kinase (JNK) activation during EMT, but CDN blocked it. Protein serine/threonine phosphatase 2A (PP2A) expression in A549 cancer cells was reduced by TGF-${\beta}1$ but CDN restored it. The overall data suggested that CDN suppresses TGF-${\beta}1$-induced EMT via PP2A restoration, making it a potential new drug candidate that controls metastasis.

Influencing Factors on Eating Disorders in College Students (대학생의 섭식장애에 영향을 미치는 요인)

  • Bang, So-Youn;Kim, Soon-Yi;Yang, Kyoung-Mi
    • The Journal of Korean Academic Society of Nursing Education
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    • v.16 no.2
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    • pp.265-271
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    • 2010
  • Purpose: The purpose of this study was to identify the influencing factors on eating disorders in college students. Method: A descriptive correlational study design was used. Eight hundred fifty-nine college students were administered self-report questionnaires to examine eating habits, self-efficacy, depression, and parent-adolescent communication from May to June, 2010. Collected data were analyzed by the SPSS 15.0 program for t-test, ANOVA, Pearson's correlation coefficient, and stepwise multiple regression. Result: The level of an eating disorder in participants was moderate and it differed by gender, major, and type of living. The eating disorder showed a significant correlation with self-efficacy (r=-.28, p=<.000), depression (r=.23, p=.001), and parents-adolescent communication (r=-.13, p=.008). Self-efficacy (8.2%) and depression (2.1%) were significant predictors to explain the eating disorder. Conclusion: This study provides comprehensive understanding of the influencing factors on eating disorders in college students. It suggests that nursing interventions to improve self-efficacy and decrease depression for management of eating disorder are needed.

Mediating Effects of Interpersonal Problems in the Relationship between Social Network Service Use Tendency and Depression among University Students (대학생의 소셜 네트워크 서비스(Social Network Service) 중독경향성과 우울의 관계에서 대인관계 문제의 매개효과)

  • Park, Min-Jeong;Chung, Mi Young
    • Research in Community and Public Health Nursing
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    • v.30 no.1
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    • pp.38-46
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    • 2019
  • Purpose: The purpose of this study isto identify the mediating effects of interpersonal problems in the relationship between Social Network Service (SNS) use tendency and depression among university students. Methods: Data were collected from April 28 to May 7, 2018 and the participants included 222 university students, who responded to the question regarding SNS use tendency, interpersonal problems and depression. The data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients, and multiple regression using the SPSS/WIN 23.0 program. Results: A positive correlation is found between depression and SNS use tendency (r=.24, p<.001), and among interpersonal problems (r=.62, p<.001), SNS use tendency and interpersonal problems (r=.34, p<.001). Interpersonal problems have a full mediating effect on the relationship between SNS use tendency and depression (Sobel test: 5.24, p<.001). Conclusion: These results suggest that it is important to manage interpersonal problems to prevent depression caused by SNS use tendency.

The Effect of CPR Knowledge, Attitude, Teacher Efficacy, and Performance Confidence of Elementary, Middle, and High School Teachers (초·중·고등학교 교사의 심폐소생술 수행 자신감에 미치는 영향)

  • Cho, Gyoo Yeong;Seo, Mi Kyung
    • Journal of Korean Public Health Nursing
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    • v.37 no.2
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    • pp.193-204
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    • 2023
  • Purpose: This study was undertaken to identify the association between CPR knowledge, attitude, and teacher efficacy on the performance confidence of CPR. We further investigated methods to enhance the performance ability of CPR among elementary, middle, and high school teachers. Methods: The study design was a descriptive survey conducted from October 15 to December 31, 2022, enrolling 155 elementary, middle, and high school teachers. The data were analyzed by applying Multiple regression, Pearson's correlation coefficient, one way ANOVA and Scheffé test using the SPSS/WIN 23.0 program. Results: The performance confidence of CPR among elementary, middle, and high school teachers showed a significant positive correlation with CPR knowledge (r=.49, p<.001), CPR attitude (r=.26, p<.001), and teacher efficacy (r=.25, p=.002). The factors affecting performance confidence in CPR were CPR knowledge (β=.49, p<.001), gender (β=-.26, p<.001), CPR attitude (β=.20, p=.003), health status (β=.14, p=.038), and teacher efficacy (β=.14, p=.032); these factors explained 40.5%. Conclusion: Results from the study indicate that performance confidence of CPR can be enhanced by providing frequent education on CPR knowledge and attitudes. Moreover, educational programs will aid in maintaining good health and enhance teacher efficacy.

Flowering and Growth of Dendrobium phalaenopsis as influenced by Photoperiod, Temperature, and Plant Growth Regulators (온도, 일장 및 식물생장조절제에 의한 덴파레의 생육 및 개화 반응)

  • Lee, Young-Ran;Kim, Mi-Seon;Kim, Jae-Yeong
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.247-254
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    • 2008
  • This study was conducted to investigate effect of temperature, photoperiod, and plant growth regulators on the growth and flowering of Dendrobium phalaenopsis. Photoperiod treatments of 10 or 16 day length were from Mar. 24 to May 23. After treatments, all plants were exposed under natural photoperiod. Temperature treatments promoted development of new shoots (leads) of Dendrobium phalaenopsis 'Semi Alba' at $30/25^{\circ}C$(day/night) for 12 weeks, and 16 hr-photoperiod treatment promoted development of new shoots and flowering. The 16 hr photoperiod Dendrobium phalaenopsis 'Candistraipe ${\times}$ Tedtakiguz' no difference in the numbers of flower, flower width, and stalk length compared to the 10 hr photoperiod. Days to first flowering from appearance of new shoot were 241 days under natural photoperiod, 243 days under 10 hr, and 216 days under 16 hr, so that 16 hr-photoperiod treatment was faster about 25 days than that of natural or 10 hr treatment. There was not significant difference in growing and flowering of Dendrobium phalaenopsis among plant growth regulators.