• Title/Summary/Keyword: miR-21

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The Validation of the Child Behavior Checklist (아동행동평가척도(CBCL)의 타당화 연구)

  • Han, Mi Hyun;Yoo, An Jin
    • Korean Journal of Child Studies
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    • v.16 no.2
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    • pp.5-21
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    • 1995
  • The purpose of this study was to validate the Child Behavior Checklist(CBCL) with a population of Korean children. Subjects were 415 mothers of fourth and sixth graders selected from five elementary schools in Seoul. The CBCL(Achenbach, 1991) and the Child Behavior Questionnaire(CBQ; Rutter et als., 1970) were used as a methodological instrument. Statistics used for data analysis were ${\chi}^2$, Cramer's V, factor analysis, Pearson's r, and Cronbach's ${\alpha}$. The major findings were that 102 items of the 107-item scale were found acceptable through the item discriminant method. Nine factors (anxious behavior, hyperactive behavior, immature behavior, delinquent behavior, aggressive behavior, withdrawn behavior, thought problems, somatic complaints, other problems) were extracted by factor analysis. The correlation between the CBCL and the CBQ was examined to test criterion-related validity, and the coefficient was significant (r=.78, p<.001). Finally, the internal consistency of the CBCL appeared to be at an acceptable level (Cronbach's ${\alpha}$=.83).

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PCR-based Specific Detection of Ralstonia solanacearum by Amplification of Cytochrome c1 Signal Peptide Sequences

  • Kang, Man-Jung;Lee, Mi-Hee;Shim, Jae-Kyung;Seo, Sang-Tae;Shrestha, Rosemary;Cho, Min-Seok;Hahn, Jang-Ho;Park, Dong-Suk
    • Journal of Microbiology and Biotechnology
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    • v.17 no.11
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    • pp.1765-1771
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    • 2007
  • A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants.

Factors Influencing Health-Promoting Behaviors in People Living with HIV (HIV 감염인의 건강증진행위에 영향을 미치는 요인)

  • Park, Young Mi;Shin, Gisoo;Kim, Jiyoung
    • Korean Journal of Adult Nursing
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    • v.26 no.2
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    • pp.234-243
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    • 2014
  • Purpose: The purpose of this study was to investigate the factors influencing the health-promoting behaviors of the people living with HIV. Methods: Descriptive cross-sectional study design was used. The study participants included 99 people living with HIV recruited via the Korea HIV/AIDS Network of Solidarity from May 15 to August 15, 2012. Self-report questionnaires were used fo data collection measuring perceived stress, depression, self-efficacy, health-promoting behaviors (HPLP-II), and the perceived health status. The analysis was done using SPSS/Win 18.0 program. Descriptive statistics, pearson correlation, t-test, ANOVA and stepwise multiple regressions were used for data analysis. Results: Perceived stress (r=-.21, p=.040), depression (r=-.37, p<.001), self-efficacy (r=.42, p<.001), perceived health status (r=.29, p=.003), and health-promoting behaviors were significantly correlated with each other. The self-efficacy, religion and perceived health status were significant factors, which explained about 26% of the variance of the health-promoting behaviors. Multiple regression analysis showed that a powerful predictor of health-promoting behaviors among people living with HIV was self-efficacy. Conclusion: This study suggested that a focus of nursing intervention program on self-efficacy is necessary in order to increase health-promoting behaviors among people living with HIV.

Vaccination of Shrimp (Penaeus chinensis) against White Spot Syndrome Virus (WSSV)

  • Ha, Yu-Mi;Gong, Soo-Jung;Nguyen, Thi-Hoai;Ra, Chae-Hun;Kim, Ki-Hong;Nam, Yoon-Kwon;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.964-967
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    • 2008
  • Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.

Perception of COVID-19, and Knowledge, Attitude and Practice of Hand washing in Elementary School Students (초등학생의 코로나19에 대한 인식과 손 씻기 지식, 태도 및 실천)

  • Lee, Young-Hee;You, Mi-Ae
    • Journal of Korean Public Health Nursing
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    • v.36 no.1
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    • pp.20-32
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    • 2022
  • Purpose: This study aimed to investigate factors affecting handwashing practice among elementary school students by assessing the perception of coronavirus disease-19 (COVID-19) and characteristics related to knowledge, attitude, and practice of handwashing. Methods: The cross-sectional study design and an online self-reported survey were used in this study. The subjects were 211 elementary students at a school located in G province, Korea. Data were collected from January 4 to 8, 2021. The data were analyzed using SPSS version 25.0. Results: Mean scores for knowledge, attitude, and practice of handwashing were 17.09 (out of 20), 21.15 (out of 24), and 27.51 (out of 33), respectively. There were significant positive correlations between the perception of COVID-19 and knowledge of handwashing (r=.23, p=.001), between knowledge and attitude toward handwashing (r=.45, p<.001), between knowledge and practice of handwashing (r=.18, p=.010), and between attitude and practice of handwashing (r=.28, p<.001). The factors influencing handwashing practice were handwashing frequency at home (β=.39, p<.001), handwashing frequency at school (β=.18, p=.006), and attitude toward handwashing (β=.15, p=.026), which explained 34.2% of the variance. Conclusion: These results suggest a need to raise awareness of the importance of handwashing and develop an education program for infectious disease prevention.

Synthesis and X-ray Crystallographic Characterization of p-Diacetylcalix[4]arene

  • Young Ja Park;Kwanghyun No;Jung Mi Shin
    • Bulletin of the Korean Chemical Society
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    • v.12 no.5
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    • pp.525-529
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    • 1991
  • A simple route is described for the selective functionalization of calixarene at the para positions of phenyl rings. Calix[4]arene tetraacetate 2, obtained from the treatment of calix[4]arene with acetic anhydride, undergoes Fries rearrangement to yield the diametrically para substituted p-diacetylcalix[4]arene 3 in 80% yield. The crystal and molecular strucutre has been determined by X-ray diffraction method. The crystals are orthorhombic, space group Pna21, with a = 11.121 (3), b = 10.374 (3), c = 21.690 (6) $\AA$ and Z = 4. The structure was solved by direct method and refined by full-matrix least-squares methods to final R of 0.036 for 1795 observed reflections. Each hydroxyl hydrogen atom is disordered over two positions. The macrocycle exists in the cone conformation which is determined by the strong circular intramolecular flip-flop type hydrogen bonds of phenolic OH, while crystal packing effects of the diametrically para-acetyl substituents seem to be responsible for the distortion of the cone conformation.

Cytomegalovirus Infection in Pediatric Renal Transplant Recipients: A Single Center Experience

  • Kim, Mi Jin;You, Ji Hye;Yeh, Hye Ryun;Lee, Jin A;Lee, Joo Hoon;Park, Young Seo
    • Childhood Kidney Diseases
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    • v.21 no.2
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    • pp.75-80
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    • 2017
  • Purpose: To investigate the frequency, presentation, management, and outcome of cytomegalovirus (CMV) infection in pediatric patients who underwent renal transplantation. Methods: We performed a retrospective chart review of 70 patients under the age of 18, who underwent renal transplantation between January 1990 and November 2014. A diagnosis of CMV infection was based on serology, molecular assays, antigenemia assays, and culture. CMV infection was defined as detection of virus and CMV disease was diagnosed when clinical signs and symptoms were present. Results: The number of patients with CMV infection was 18 (25.7% of renal transplant recipients). Twelve were male (66.7%), and the $mean{\pm}standard$ deviation (SD) age at infection was $13.3{\pm}3.9$ years. Median time of infection after renal transplantation was 4 months (range 1.0-31.0 months). Pretransplantation CMV status in the infected group was as follows: donor (D)+/recipient (R)+, 11 (61.1%); D+/R-, 7 (38.9%); D-/R+, 0; and D-/R- 0. Nine patients had CMV disease with fever, leukopenia, thrombocytopenia, or organ involvement such as enteritis, hepatitis, and pneumonitis. The age of disease occurrence was $13.1{\pm}3.9$ years and the median time to disease onset after renal transplantation was 8 months (range 1.0-31.0). Immunosuppressive agents were reduced or discontinued in 14 patients (77.8%), antiviral agents were used in 11 patients (61.1%), and all patients with CMV infection were controlled. Conclusions: A quarter of the patients had CMV infection about 4 months after renal transplantation. CMV infection was successfully treated with reduction of immunosuppressants or with antiviral agents.

Factors Affecting Performance of Hand Hygiene by Parents of Hospitalized Infants (입원 영유아 부모의 손 위생 수행 영향요인)

  • Jin, Su Jin;Song, Young Mi;Han, Hye Young;Seo, Kyung Hwa;Kim, Soon Jeong
    • Child Health Nursing Research
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    • v.21 no.4
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    • pp.285-292
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    • 2015
  • Purpose: Purpose of this study was to identify factors affecting the performance of hand hygiene by parents of hospitalized infants. Methods: The study was conducted with 209 parents of hospitalized infants from one hospital in Changwon. Data were collected from June through August, 2014 and analyzed using SPSS Win 18.0 program. Results: There were positive correlations between performance of hand hygiene and perceptions of sanitary formula bottle (r=.586, p<.001), attitude to hand hygiene (r=.438, p<.001), and knowledge of hand hygiene (r=.405, p<.001) and between parent efficacy and perceptions of sanitary formula bottle (r=.572, p<.001). Awareness of perceptions of sanitary formula bottle (${\beta}$=.308, p<.001), parent efficacy (${\beta}$=.281, p<.001) and hand hygiene attitude (${\beta}$=.207, p<.001) were significant predictors of hand hygiene performance and explained 46.1% of the variance. Conclusion: To increase infection control medical institutions should provide education on hand hygiene and training for parents of hospitalized infants. In order to enhance the performance of hand hygiene, it is necessary to develop and apply nursing programs for hospitalized infants that include hand hygiene attitude, maintenance of the sanitary formula bottle and parent efficacy.

The Influence of Empathy and Leadership on Happiness in Nursing College Students (간호대학생의 공감과 리더십이 행복감에 미치는 영향)

  • Cho, Young-Mi
    • The Journal of the Korea Contents Association
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    • v.21 no.10
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    • pp.622-629
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    • 2021
  • This study was attempted to find out the effect of empathy and leadership of nursing college students on happiness. For this study, from November to December 2020, 142 students were participated from C University in U City, using self reported questionnaire. Descriptive statistics, t-test, ANOVA, Scheffe test, Pearson's correlation analysis, and multiple regression analysis were used for data analysis. As results of this study, it was revealed that there was a significant positive correlation between the happiness of nursing students on empathy and leadership(r=.424, p<.001, r=.635, p<.001). Also, the factors affecting the happiness of nursing students were personality(β=-0.284, p<.001), health status(β=-0.375, p<.001, β=-0.178, p<.001), empathy(β=0.351, p<.001) and leadership(β=0.133, p=.039) with a total explanatory power of 58.1%. As the results of this study, in order to increase the happiness of nursing students, it is necessary to develop their empathy skills and leadership. The specific strategies are to develope and apply special programs to develop the empathy skills and leadership.

The Influence of Biomedical Ethics and Critical Thinking Disposition on Nursing Professionalism in Nursing College Students (간호대학생의 생명의료윤리의식과 비판적 사고성향이 간호전문직관에 미치는 영향)

  • Cho, Young-Mi
    • The Journal of the Korea Contents Association
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    • v.21 no.11
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    • pp.638-645
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    • 2021
  • This study was attempted to find out the effect of biomedical ethics and critical thinking disposition of nursing college students on professionalism. For this study, from May to June 2021, 144 students were participated from S University in A City, using self reported questionnaire. Descriptive statistics, t-test, ANOVA, Scheffe test, Pearson's correlation analysis, and multiple regression analysis were used for data analysis. As results of this study, it was revealed that there was a significant positive correlation between the professionalism of nursing students on biomedical ethics and critical thinking disposition(r=.400, p<.001, r=.431, p<.001). Also, the factors affecting the professionalism of nursing students were grade(𝛽=0.303, p<.001), biomedical ethics(𝛽=0.287, p<.001) and critical thinking disposition(𝛽=0.294, p<.001) with a total explanatory power of 31.2%. As the results of this study, in order to increase the professionalism of nursing students, it is necessary to develop their biomedical ethics and critical thinking disposition. The specific strategies are to make and apply special programs by their grades.